Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,619,338	C→T	R370R (CGC→CGT)	deoA →	thymidine phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,619,338	0	C	T	100.0%	70.3 / NA	26	R370R (CGC→CGT)	deoA	thymidine phosphorylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/17);  total (9/17)

CGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAGGCGGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCGACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGG‑GGATGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCA  >  NC_000913/4619097‑4619570                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                         cGAAAGATGACGCCGACGCGCGCGCGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGACATGGATACCCGCGCGCTGG‑GGATGGCAGTGGTTGCAATGGGAAGCGGTCGTCGTCAGGc                                                                                                                                                                                                                                  >  1:51015/1‑250 (MQ=255)             ccGAAGTGCGCGACAAATTGCAAGCAGTGTTGGGCAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGCAGCGGCTAAAAAAGGCCCATCCGACCTCGCGGAGAACTGCGCGAAGTATCTGCCGCCCGCGATGCTGACGAAAGACGTCGACGCTGATACCGAAGGTTTTGGCAGTGAAATGGATACCCGCGC‑CTGGTGGATGGCGGTGGTTGCAAGGGGCGGCGGTCGTCGACAGGCacatgacatgagt                                                                                                                                                                                                                     <  2:106300/251‑13 (MQ=255)                  gcgcgcgcGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCGGATACCGAAGGTTTTGTCAGGGAAATGGATACCCGCGCGCTGG‑GGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTa                                                                                                                                                                                                                <  2:17319/251‑1 (MQ=255)                       cgcgAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGG‑GGAGGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCg                                                                                                                                                                                                           >  1:152487/1‑251 (MQ=255)                          gAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGG‑GGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCg                                                                                                                                                                                                        <  2:92664/251‑1 (MQ=255)                            aaTTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGG‑GGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGc                                                                                                                                                                                                      <  1:93857/251‑1 (MQ=255)                             aTTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGGCGTATGGTAGCGGCACAAAAAGGCCCAACCGACGTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTGTTGTCAGTGAAATGGATACCCGCGCGCTGG‑GGAGGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCt                                                                                                                                                                                                     <  2:13513/251‑1 (MQ=255)                                                 aCGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGG‑GGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGtttg                                                                                                                                                                                 <  1:230908/251‑4 (MQ=255)                                                 aCGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGG‑GGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGtttg                                                                                                                                                                                 <  2:208980/251‑4 (MQ=255)                                                                aaGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTGCGGTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGAGACCCGCGCGCTGG‑GGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGAc                                                                                                                                                                  <  2:237023/251‑1 (MQ=255)                                                                      atGGTCGTATGGTAGCGGCACAAAAAGGCCCAGCCGTCGTCGTTGAGAACTACGCGAAGGATCGGCCGACAGCGATGCTGACTAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGG‑GGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCAGCTGACACCAGTGATGACAGCGTCGGCTTGTCTGATAAGGCGCGTTTGGGCGACCAGGTAGACGGTc                                                                                                                                                              <  2:249418/248‑1 (MQ=255)                                                                       tGGTCGTAAGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGCACTACGCGAAGTATCTGCCGCCAGCGATGCTGACGAACGCAGTCTATGCTGCAACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGG‑GGATGGCAGTGGTTGAAATAGGCGGCGGTCGTCGTCCGACATCTGACACCATTGATTACAGCGTCGGCTTTACTGCTATGGCCCGTTTGGGCGACCAGATAGACggac                                                                                                                                                              >  1:172634/1‑246 (MQ=255)                                                                                                ggCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGG‑GGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGaa                                                                                                                                  <  1:133100/251‑1 (MQ=255)                                                                                                              cGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGG‑GGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGGAaacagct                                                                                                                    >  1:69913/1‑248 (MQ=255)                                                                                                                                               gATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGG‑GGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCCTTGATTACAGCGTCGGCTTTACTGATATGGCGCGGTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAATGCGAAAACAGCCGGCAGGAAGGGGCGaaaaggggggaagcggggc                                                                                   >  2:135155/1‑235 (MQ=255)                                                                                                                                                       cGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGG‑GGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGGCGAAAACAGCTGGCAGGAAGCGGCGAAAACGGTGAAAGCGGGAATTaaaatt                                                                           >  2:70286/1‑248 (MQ=255)                                                                                                                                                                       tGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGG‑GGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGAAGAAAAAAGCTGGCAGGAAGCGGGGAAAGCGGTGGAAGCGGCAATTAAACTTGCCGATAAAGAACCgg                                                           >  1:177147/1‑251 (MQ=255)                                                                                                                                                                                             gAAATGGATTCCCGCGCGCTGG‑GGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCg                                     <  2:83814/251‑1 (MQ=255)                                                                                                                                                                                                      ccccGCGCGCTGG‑GGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAg                            <  1:1020/250‑1 (MQ=255)                                                                                                                                                                                                           cgggcTGG‑GGATGGCAGTGGTTGCAATGGGCAGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGTCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGATGGCGGTTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCTGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGGCTAGCGCCGTATCAGCGAAt                       <  2:51015/248‑1 (MQ=255)                                                                                                                                                                                                            gcgcTGG‑GGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGGCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATa                      <  1:132595/251‑1 (MQ=255)                                                                                                                                                                                                                tGG‑GGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATCTGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGAGAAAGACGAAAACAGCTGGCAGGGAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACgg                  >  1:157100/1‑251 (MQ=255)                                                                                                                                                                                                                 gg‑ggATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGGTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACgg                  <  1:182724/250‑1 (MQ=255)                                                                                                                                                                                                                          cAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCCCGCGAAAGACg                                                                                                                             >  1:81677/1‑135 (MQ=255)                                                                                                                                                                                                                          cAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACg                                                                                                                             <  2:81677/135‑1 (MQ=255)                                                                                                                                                                                                                                  gCAATGGGCGGCTGTCGTCGTCAGGCATCTGACACCATTGAGTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGGCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCa  <  2:146923/250‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                         CGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAGGCGGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCGACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGG‑GGATGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCA  >  NC_000913/4619097‑4619570

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑