Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,618,489	C→T	T87T (ACC→ACT)	deoA →	thymidine phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,618,489	0	C	T	96.4%	80.9 / NA	28	T87T (ACC→ACT)	deoA	thymidine phosphorylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (17/10);  minor base G (0/1);  total (17/11)
Fisher's exact test for biased strand distribution p-value = 3.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

AGAAATTATTCGTAAAAAACGTGATGGTCATGCGCTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGTATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACACTCCACCGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACAAACTGGAATCCATCCCTGGCTTCGACATTTTCCCGGATGACAACC‑GTTTCCGCGAAATTATT‑AAAGACGTCGGCGTGGCGATTATCGGTCAGACCAGTTCACTGGCTCCGGCTGATAAACGT  >  NC_000913/4618243‑4618726                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                aGAAATTATTCGTAAAAAACGTGATGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTg                                                                                                                                                                                                                                             >  2:51356/1‑251 (MQ=255)   aaaTTAGTCTTAAAAAACGTGATTGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATTACCATTTGCTTCCGCGATAGGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTGTCGACTGTAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAGCATTCCACGGGTGGc                                                                                                                                                                                                                                           <  2:118536/251‑1 (MQ=255)                        aTGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTACATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTg                                                                                                                                                                                                                      >  1:56203/1‑251 (MQ=255)                                     gAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGcc                                                                                                                                                                                                           <  2:5179/249‑1 (MQ=255)                                        cGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATgg                                                                                                                                                                                                      <  1:255272/251‑1 (MQ=255)                                          aTGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGCTGGTc                                                                                                                                                                                                    >  2:4776/1‑251 (MQ=255)                                             aagaaATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCACTAGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAACGCCTGCATCTGACTGGCCCGATTGTTGATAAACATTCCCCTGGTGGAGTCGGCGATGTAACTTCGCTGCTACTGGGGCCGATGGTCGc                                                                                                                                                                                                  >  2:106495/1‑250 (MQ=255)                                                                cAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTc                                                                                                                                                                              >  1:54890/1‑251 (MQ=255)                                                                                      aTCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGc                                                                                                                                                          <  2:54890/249‑1 (MQ=255)                                                                                          ccGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTc                                                                                                                                                       <  1:24086/248‑1 (MQ=255)                                                                                             aaGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATTTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGGGGCCTCGGtaat                                                                                                                                                 >  1:149722/1‑248 (MQ=255)                                                                                                ggCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCTATGATCTCTGGTCGCGGCCGTGGTCATACt                                                                                                                                              <  2:254859/251‑1 (MQ=255)                                                                                                  cAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAGCCGTTCTCGCCTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAGCATTCCACTGGTGGCGTCGGCGATGTGCCTTCGCTGATGTTGGGGACGATGGTCGCAGCCTGCGGCGGCGATATTTCGATGTTCTCTGGGCGCGGCCTCGGTCCTACTgg                                                                                                                                            >  2:120889/1‑251 (MQ=255)                                                                                                        gccgccCTCGCTTGGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGAGGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAACCATTCCACTGGTGGCGTCGGCGATGTTACTTCGCTGATGTTGTGGCCGATGGTCGCAGCCTGCTGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCTGTCATACTGGCGGTAc                                                                                                                                      <  2:227305/251‑1 (MQ=255)                                                                                                                            tttCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACCGGAGGTTCGCTCGACCAAATGGAatccc                                                                                                                  >  2:76317/1‑250 (MQ=255)                                                                                                                               cttcCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGCTTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCCTACTGGCGGTAAGCTCGGCAAACTGGGatccatcc                                                                                                               >  1:193913/1‑251 (MQ=255)                                                                                                                                ttcCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGGTGGTTTCTGGTCGGGGCCCTGGTCATACTGGTGGTAccgccgacaaaataggatcacttcc                                                                                                              >  1:68810/1‑227 (MQ=255)                                                                                                                                  ccACGATATGACACTGCCTGCGCGTGTCTCGCTGCCCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGGACgtccgctccaatggactccctccccg                                                                                                            >  2:126544/1‑226 (MQ=255)                                                                                                                                                          tgtCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTTTATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGGGGGAGGACCGACAAACTGGAATCCATCCCTGGCTTCGACATTTTTCCGGATGAc                                                                                     >  1:31577/1‑250 (MQ=255)                                                                                                                                                                gCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCAGACTGTCGGTACGCTCGACAAACTGGAATCCACCCCTGGCTTCGACATTTTCCCGGATGACAACC‑Gtt                                                                             <  2:238868/251‑1 (MQ=255)                                                                                                                                                                                 gagaTTCAGGCACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGCCAACCTGGAATCCCTACCTGGCTTCCACATTTTCCCGGATGACAAAC‑GCTTCCGCGCAATTATT‑aa                                                            >  2:235125/1‑250 (MQ=255)                                                                                                                                                                                        aGGAACCTTTCTCGACTGGAAAAGCCTTCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACAAACTGGAATCCATCCCTGGCTTCGACATTTTCCCGGATGACAACC‑GTTTCCGCGAAATTATG‑AAAGACGTCg                                                    <  2:172686/251‑1 (MQ=255)                                                                                                                                                                                               gTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACCAACAGGGATCCACCCCTGGCTTTGCCATTTTTCCGGAAGACAACC‑GTTTTCGCGAAATTTTT‑TAAGACGTCggactggg                                             >  2:209326/1‑245 (MQ=255)                                                                                                                                                                                               gTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACCCTCCAACAACCGGAATCCCACCCCCGCTTCGACATTTTCCCGGCTGAAAACCCGTTTC‑GCCAAAATTTT‑AAAGAccgtgggggggg                                             >  2:191348/1‑240 (MQ=255)                                                                                                                                                                                                        tGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGGTCGACAAACTGGAATCCCTCCCTGGCTTTGACATTTTTCCGGATGACAACC‑GGTTTCGCCGAATTATT‑TAAAACGTCgggggggggattttccg                                    >  2:268154/1‑236 (MQ=255)                                                                                                                                                                                                             aaGCCTGCATATGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACAAACTGGAATCCATCCCTGGCTTCGGCATTTTCCCGGATTACAAAC‑GTTTCCGCGAAATTATTTAAAG‑CGTCGGCggtgcggatatcggttca                                >  1:68695/1‑233 (MQ=255)                                                                                                                                                                                                                                        tgATAAACATTCCACTGGTGGCGTCGGCGATGTGCCGTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACAAACTGGAATCCATCCCTGGCTTCGACATTTTCCCGGATGACAACC‑GTTTCCGCGAAATTATT‑AAAGACGTCGGCGTGGCGATTATCGGTCAGACCAGCTCACTGGCTCCGGCGGATaaa     <  1:106495/250‑1 (MQ=255)                                                                                                                                                                                                                                          aTAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACAAACTGGAATCCATCCCTGGCTTCGACATTTTCCCGGATGACAACC‑GTTTCCGCGAAATTATT‑AAAGACGTCGGCGTGGCGATTATCGGTCAGACCAGCTCACTGGCTCCGGCGGATAAACGt  <  1:206594/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                AGAAATTATTCGTAAAAAACGTGATGGTCATGCGCTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGTATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACACTCCACCGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACAAACTGGAATCCATCCCTGGCTTCGACATTTTCCCGGATGACAACC‑GTTTCCGCGAAATTATT‑AAAGACGTCGGCGTGGCGATTATCGGTCAGACCAGTTCACTGGCTCCGGCTGATAAACGT  >  NC_000913/4618243‑4618726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑