Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,618,483	C→T	H85H (CAC→CAT)	deoA →	thymidine phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,618,483	0	C	T	96.7%	87.4 / NA	30	H85H (CAC→CAT)	deoA	thymidine phosphorylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (17/12);  minor base G (0/1);  total (17/13)
Fisher's exact test for biased strand distribution p-value = 4.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

CGCACAAGAAATTATTCGTAAAAAACGTGATGGTCATGCGCTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGTATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACACTCCACCGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACAAACTGGAATCCATCCCTGGCTTCGACATTTTCCCGGATGACAACC‑GTTTCCGCGAAATTATT‑AAAGACGTCGGCGTGGCGATTATCGGTCAGACCAGTTCACTGGCTCCGGCTGATAAACGT  >  NC_000913/4618237‑4618726                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      cGCACAAGAAATTATTCGTAAAAAACGTGATGGTCATGCACTGAGCGATTAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACTCTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTcc                                                                                                                                                                                                                                                    <  1:192610/250‑1 (MQ=255)       aGAAATTATTCGTAAAAAACGTGATGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTg                                                                                                                                                                                                                                             >  2:51356/1‑251 (MQ=255)       aGAAAGTATTCTTAAAAAACGTGATGGTCTTGCACTTAGCGATGAATAAATCCGTTTCTTTATCATCGGCATTCGCGACAACTCTATCTCCGAATGGCAGATTGCCGCCCTCGCGATTACCATTTTCTTCCACGATATGACAATGCCTGATCGGGTGTCGCTGACCATGGCGAGGCTGGATTGAGGATCCGTTCTCGACTGGAAAATCCTGCATCTGAATGTTCCGATGTTTGATAAACAGTCCActagtg                                                                                                                                                                                                                                             <  2:129171/251‑6 (MQ=255)         aaaTTAGTCTTAAAAAACGTGATTGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATTACCATTTGCTTCCGCGATAGGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTGTCGACTGTAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAGCATTCCACGGGTGGc                                                                                                                                                                                                                                           <  2:118536/251‑1 (MQ=255)                              aTGGTCATGCACTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTACATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTg                                                                                                                                                                                                                      >  1:56203/1‑251 (MQ=255)                                           gAGCGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGcc                                                                                                                                                                                                           <  2:5179/249‑1 (MQ=255)                                              cGATGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATgg                                                                                                                                                                                                      <  1:255272/251‑1 (MQ=255)                                                aTGAAGAAATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGCTGGTc                                                                                                                                                                                                    >  2:4776/1‑251 (MQ=255)                                                   aagaaATTCGTTTCTTTATCAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCACTAGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAACGCCTGCATCTGACTGGCCCGATTGTTGATAAACATTCCCCTGGTGGAGTCGGCGATGTAACTTCGCTGCTACTGGGGCCGATGGTCGc                                                                                                                                                                                                  >  2:106495/1‑250 (MQ=255)                                                                      cAACGGCATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTc                                                                                                                                                                              >  1:54890/1‑251 (MQ=255)                                                                                            aTCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGc                                                                                                                                                          <  2:54890/249‑1 (MQ=255)                                                                                                ccGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTc                                                                                                                                                       <  1:24086/248‑1 (MQ=255)                                                                                                   aaGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATTTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGGGGCCTCGGtaat                                                                                                                                                 >  1:149722/1‑248 (MQ=255)                                                                                                      ggCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCTATGATCTCTGGTCGCGGCCGTGGTCATACt                                                                                                                                              <  2:254859/251‑1 (MQ=255)                                                                                                        cAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAGCCGTTCTCGCCTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAGCATTCCACTGGTGGCGTCGGCGATGTGCCTTCGCTGATGTTGGGGACGATGGTCGCAGCCTGCGGCGGCGATATTTCGATGTTCTCTGGGCGCGGCCTCGGTCCTACTgg                                                                                                                                            >  2:120889/1‑251 (MQ=255)                                                                                                              gccgccCTCGCTTGGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGAGGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAACCATTCCACTGGTGGCGTCGGCGATGTTACTTCGCTGATGTTGTGGCCGATGGTCGCAGCCTGCTGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCTGTCATACTGGCGGTAc                                                                                                                                      <  2:227305/251‑1 (MQ=255)                                                                                                                                  tttCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACCGGAGGTTCGCTCGACCAAATGGAatccc                                                                                                                  >  2:76317/1‑250 (MQ=255)                                                                                                                                     cttcCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGCTTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCCTACTGGCGGTAAGCTCGGCAAACTGGGatccatcc                                                                                                               >  1:193913/1‑251 (MQ=255)                                                                                                                                      ttcCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGGTGGTTTCTGGTCGGGGCCCTGGTCATACTGGTGGTAccgccgacaaaataggatcacttcc                                                                                                              >  1:68810/1‑227 (MQ=255)                                                                                                                                        ccACGATATGACACTGCCTGCGCGTGTCTCGCTGCCCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGGACgtccgctccaatggactccctccccg                                                                                                            >  2:126544/1‑226 (MQ=255)                                                                                                                                                                tgtCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTTTATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGGGGGAGGACCGACAAACTGGAATCCATCCCTGGCTTCGACATTTTTCCGGATGAc                                                                                     >  1:31577/1‑250 (MQ=255)                                                                                                                                                                      gCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCAGACTGTCGGTACGCTCGACAAACTGGAATCCACCCCTGGCTTCGACATTTTCCCGGATGACAACC‑Gtt                                                                             <  2:238868/251‑1 (MQ=255)                                                                                                                                                                                       gagaTTCAGGCACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGCCAACCTGGAATCCCTACCTGGCTTCCACATTTTCCCGGATGACAAAC‑GCTTCCGCGCAATTATT‑aa                                                            >  2:235125/1‑250 (MQ=255)                                                                                                                                                                                              aGGAACCTTTCTCGACTGGAAAAGCCTTCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACAAACTGGAATCCATCCCTGGCTTCGACATTTTCCCGGATGACAACC‑GTTTCCGCGAAATTATG‑AAAGACGTCg                                                    <  2:172686/251‑1 (MQ=255)                                                                                                                                                                                                     gTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACCAACAGGGATCCACCCCTGGCTTTGCCATTTTTCCGGAAGACAACC‑GTTTTCGCGAAATTTTT‑TAAGACGTCggactggg                                             >  2:209326/1‑245 (MQ=255)                                                                                                                                                                                                     gTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACCCTCCAACAACCGGAATCCCACCCCCGCTTCGACATTTTCCCGGCTGAAAACCCGTTTC‑GCCAAAATTTT‑AAAGAccgtgggggggg                                             >  2:191348/1‑240 (MQ=255)                                                                                                                                                                                                              tGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGGTCGACAAACTGGAATCCCTCCCTGGCTTTGACATTTTTCCGGATGACAACC‑GGTTTCGCCGAATTATT‑TAAAACGTCgggggggggattttccg                                    >  2:268154/1‑236 (MQ=255)                                                                                                                                                                                                                   aaGCCTGCATATGAATGGCCCGATTGTTGATAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACAAACTGGAATCCATCCCTGGCTTCGGCATTTTCCCGGATTACAAAC‑GTTTCCGCGAAATTATTTAAAG‑CGTCGGCggtgcggatatcggttca                                >  1:68695/1‑233 (MQ=255)                                                                                                                                                                                                                                              tgATAAACATTCCACTGGTGGCGTCGGCGATGTGCCGTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACAAACTGGAATCCATCCCTGGCTTCGACATTTTCCCGGATGACAACC‑GTTTCCGCGAAATTATT‑AAAGACGTCGGCGTGGCGATTATCGGTCAGACCAGCTCACTGGCTCCGGCGGATaaa     <  1:106495/250‑1 (MQ=255)                                                                                                                                                                                                                                                aTAAACATTCCACTGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACAAACTGGAATCCATCCCTGGCTTCGACATTTTCCCGGATGACAACC‑GTTTCCGCGAAATTATT‑AAAGACGTCGGCGTGGCGATTATCGGTCAGACCAGCTCACTGGCTCCGGCGGATAAACGt  <  1:206594/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      CGCACAAGAAATTATTCGTAAAAAACGTGATGGTCATGCGCTGAGCGATGAAGAAATTCGTTTCTTTATCAACGGTATTCGCGACAACACTATCTCCGAAGGGCAGATTGCCGCCCTCGCGATGACCATTTTCTTCCACGATATGACAATGCCTGAGCGTGTCTCGCTGACCATGGCGATGCGAGATTCAGGAACCGTTCTCGACTGGAAAAGCCTGCATCTGAATGGCCCGATTGTTGATAAACACTCCACCGGTGGCGTCGGCGATGTGACTTCGCTGATGTTGGGGCCGATGGTCGCAGCCTGCGGCGGCTATATTCCGATGATCTCTGGTCGCGGCCTCGGTCATACTGGCGGTACGCTCGACAAACTGGAATCCATCCCTGGCTTCGACATTTTCCCGGATGACAACC‑GTTTCCGCGAAATTATT‑AAAGACGTCGGCGTGGCGATTATCGGTCAGACCAGTTCACTGGCTCCGGCTGATAAACGT  >  NC_000913/4618237‑4618726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑