Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,618,055	C→T	L245L (CTG→TTG)	deoC →	deoxyribose‑phosphate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,618,055	0	C	T	100.0%	98.1 / NA	32	L245L (CTG→TTG)	deoC	deoxyribose‑phosphate aldolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/17);  total (15/17)

TGAACGCGACGCCGGAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCGGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATTGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTG‑AAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGATGGTCATGCGCTGAGCGATGAAGA  >  NC_000913/4617846‑4618290                                                                                                                                                                                                                  |                                                                                                                                                                                                                                             tgAACGCGACGCCGGAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTG‑AAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCagcagc                                                                                                                                                                                                    >  2:205030/1‑251 (MQ=255)              ggAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGACAAAACCGTTGGTTTCAAACAAGCGGGCGGCGTGCGTCCTGCGGAAGATGCGCAGAAATACCTCGCCATCGCAGCTGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCCAGCCTGTTG‑AAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACtaagta                                                                                                                                                                                           >  1:129171/1‑247 (MQ=255)               gAAAGCGCGCGCATCATGATGGTAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGGCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTG‑AAAGCGCTGGGCCACGTTGACGGTAAGAGCGCCAGCAGATACTAAGTAAGATg                                                                                                                                                                                      <  2:55223/251‑1 (MQ=255)                aaaGCGCGCGCATCATGATGGAAGCGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCTCTACCGCTTGGGTGCTGCCAGCCTGCGGGCAAGCCT‑TTGTAAAGCGCAGGGCCACTGTGACGGTGAGAGCGCCAGCAGCTACTAAGTAAGATg                                                                                                                                                                                      <  2:257429/250‑1 (MQ=255)                             catGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTG‑AAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGAtg                                                                                                                                                                        <  1:201688/251‑1 (MQ=255)                                 atgtaaGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTG‑AAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGAtgcgct                                                                                                                                                                    <  1:244635/247‑1 (MQ=255)                                   ggAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTG‑AAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGAtgcgctgc                                                                                                                                                                  <  1:56678/251‑1 (MQ=255)                                   ggAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTG‑AAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGAtgcgctg                                                                                                                                                                   <  1:11707/250‑1 (MQ=255)                                       gTGATCCGGGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTG‑AAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCtt                                                                                                                                                              <  2:148676/251‑1 (MQ=255)                                                    tGGGCGGAGGAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTAATGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTG‑AAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACg                                                                                                                                                  >  2:24086/1‑250 (MQ=255)                                                          tAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGAGCTTCCAGCCTGCTGGCAAGCCTGTTG‑ACAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGt                                                                                                                                            >  1:24665/1‑250 (MQ=255)                                                                     gTTGGTTTCAACCCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTG‑AAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCGTATCAGGCCTACGAGACGTATCTACCCGTAg                                                                                                                                <  1:96969/251‑1 (MQ=255)                                                                       tgggttCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGAGGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGTCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGTCAAGCCTGTTG‑AAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGTGCGTATCAGGCCTACGAGACGTAGCTACCCGTAgg                                                                                                                               <  2:28562/246‑1 (MQ=255)                                                                                            cgtgcgtACTGCGGAAGAGGCGCAGAAAGATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTTCTTCCAGCCTGCTGGCAAGCCTGTTG‑AAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATc                                                                                                         <  2:64804/251‑1 (MQ=255)                                                                                                 gtACTGCGGAAGATGCGCAGAAATATCTCGCCATCGGAAATGAACTGTTCCGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTG‑AAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACTCCTGATTCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCTTAGGCCGGATAAGGCGTAGACGCATCCGGCa                                                                                                    <  1:24663/251‑1 (MQ=255)                                                                                                 gtACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATCAAATGTACGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTAGGTGCTTCCAGCCTGATGGCAAGCCTGTTG‑CAAGCGCTGGGCCACGGAGACGGTAAGAGCGCCAGCCGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCCGGCCTACGAGACGTATCTACCCGTCGGCCGGATAAGGCGTAGACGCATCCGGc                                                                                                     >  1:160517/1‑250 (MQ=255)                                                                                                         gAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTG‑AAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGCCGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAgccgc                                                                                            >  1:118536/1‑251 (MQ=255)                                                                                                                       atatCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTG‑AAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGACGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTc                                                                              >  1:227305/1‑251 (MQ=255)                                                                                                                        tatCTCGCCATCGCAGATGACCTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTG‑ACAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCAGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCCAAAGCCGCCTCATACTCTTTTcc                                                                             >  2:192610/1‑251 (MQ=255)                                                                                                                           cgcgccttcgctgatgatCTGTTTGGCGCTGACGGGGCAGATGCGCGTCACTACCGCTTTGGTGCATCCAGCCTGCTGGCAATCCTTTTG‑AAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCATCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGGTGCGCTAATCAGGCCTAGGAGACGTACCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAGAAGCCGCCGTATACTCGTTTCCTc                                                                           <  2:235873/238‑1 (MQ=255)                                                                                                                             cGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTG‑AAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCggg                                                                        >  1:5179/1‑251 (MQ=255)                                                                                                                               ccATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTG‑AAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCggg                                                                        >  1:172686/1‑249 (MQ=255)                                                                                                                                        aTGAACGGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTG‑AAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTg                                                             <  2:3138/251‑1 (MQ=255)                                                                                                                                               gTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTG‑AAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTtctc                                                       <  2:88928/250‑1 (MQ=255)                                                                                                                                                        tGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTG‑AAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGaa                                              >  2:4242/1‑250 (MQ=255)                                                                                                                                                              ggCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTG‑AAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTAc                                                                                                                                                   >  2:213740/1‑143 (MQ=255)                                                                                                                                                              ggCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTG‑AAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTc                                       >  1:103298/1‑251 (MQ=255)                                                                                                                                                                              tACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTG‑AAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGATgg                       >  1:254859/1‑251 (MQ=255)                                                                                                                                                                                   cTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTG‑AAAGCGCTGGGCCACGGTGACGGTAAGCGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGATGGTCATg                  >  2:156781/1‑251 (MQ=255)                                                                                                                                                                                             tCCAGCCTGCTGGCAAGCCTGTTG‑AAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACGAAGTAATGTGCTGTACGCCTGATGCGCTGCGCTTATCAGTCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCACGGGAGGTTACCTTGTTTCTCGCACAATAAATTATTCGTAAAAAACGTGATGGTCATGCACTGAGCg         <  2:237643/250‑1 (MQ=255)                                                                                                                                                                                               cAGCCTGCTGGCAAGCCTGTTG‑AAAGCGCTGGGCCAAGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGATGGTCATGCACTGAGCGATg      <  1:197886/251‑1 (MQ=255)                                                                                                                                                                                                   cggctgGCAAGCCTGTTG‑AAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTAGCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGATGGTCAAGCACTGAGCGATgaaga  <  2:114473/249‑1 (MQ=255)                                                                                                                                                                                                                  |                                                                                                                                                                                                                                             TGAACGCGACGCCGGAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCGGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATTGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTG‑AAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGATGGTCATGCGCTGAGCGATGAAGA  >  NC_000913/4617846‑4618290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑