Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,618,030	C→T	G236G (GGC→GGT)	deoC →	deoxyribose‑phosphate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,618,030	0	C	T	96.7%	81.9 / NA	30	G236G (GGC→GGT)	deoC	deoxyribose‑phosphate aldolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (14/15);  minor base A (1/0);  total (15/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

AAATCTCCATCAAAGCGGGTGCGGACTTCATCAAAACCTCTACCGGTAAAGTGGCTGTGAACGCGACGCCGGAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCGGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATTGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTG‑AAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGATGGTCATG  >  NC_000913/4617789‑4618274                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      aaaTCTCCATCAAAGCGGGTGCGGACTTCATCAAATCCTCTTCCGGTAAAGTGGCTGTTATCGCGAGGCCGGAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACGGCGGAAGATGCGCATAAATATCTCGCCATCGCAGAGGAGCTGTTCGGTGCTGACTGGGCAGATGCGCGGCTCTACCGCTTTGGTGCTTCCAGc                                                                                                                                                                                                                                              <  2:130476/251‑1 (MQ=255)                                                          tgAACGCGACGCCGGAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTG‑AAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCagcagc                                                                                                                                                                                    >  2:205030/1‑251 (MQ=255)                                                                       ggAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGACAAAACCGTTGGTTTCAAACAAGCGGGCGGCGTGCGTCCTGCGGAAGATGCGCAGAAATACCTCGCCATCGCAGCTGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCCAGCCTGTTG‑AAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACtaagta                                                                                                                                                                           >  1:129171/1‑247 (MQ=255)                                                                        gAAAGCGCGCGCATCATGATGGTAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGGCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTG‑AAAGCGCTGGGCCACGTTGACGGTAAGAGCGCCAGCAGATACTAAGTAAGATg                                                                                                                                                                      <  2:55223/251‑1 (MQ=255)                                                                         aaaGCGCGCGCATCATGATGGAAGCGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCTCTACCGCTTGGGTGCTGCCAGCCTGCGGGCAAGCCT‑TTGTAAAGCGCAGGGCCACTGTGACGGTGAGAGCGCCAGCAGCTACTAAGTAAGATg                                                                                                                                                                      <  2:257429/250‑1 (MQ=255)                                                                                      catGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTG‑AAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGAtg                                                                                                                                                        <  1:201688/251‑1 (MQ=255)                                                                                          atgtaaGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTG‑AAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGAtgcgct                                                                                                                                                    <  1:244635/247‑1 (MQ=255)                                                                                            ggAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTG‑AAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGAtgcgctgc                                                                                                                                                  <  1:56678/251‑1 (MQ=255)                                                                                            ggAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTG‑AAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGAtgcgctg                                                                                                                                                   <  1:11707/250‑1 (MQ=255)                                                                                                gTGATCCGGGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTG‑AAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCtt                                                                                                                                              <  2:148676/251‑1 (MQ=255)                                                                                                             tGGGCGGAGGAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTAATGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTG‑AAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACg                                                                                                                                  >  2:24086/1‑250 (MQ=255)                                                                                                                   tAGAAAAAACCGTTGGTTTCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGAGCTTCCAGCCTGCTGGCAAGCCTGTTG‑ACAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGt                                                                                                                            >  1:24665/1‑250 (MQ=255)                                                                                                                              gTTGGTTTCAACCCAGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTG‑AAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCGTATCAGGCCTACGAGACGTATCTACCCGTAg                                                                                                                <  1:96969/251‑1 (MQ=255)                                                                                                                                tgggttCAAACCAGCGGGCGGCGTGCGTACTGCGGAAGAGGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGTCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGTCAAGCCTGTTG‑AAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGTGCGTATCAGGCCTACGAGACGTAGCTACCCGTAgg                                                                                                               <  2:28562/246‑1 (MQ=255)                                                                                                                                                     cgtgcgtACTGCGGAAGAGGCGCAGAAAGATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTTCTTCCAGCCTGCTGGCAAGCCTGTTG‑AAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATc                                                                                         <  2:64804/251‑1 (MQ=255)                                                                                                                                                          gtACTGCGGAAGATGCGCAGAAATATCTCGCCATCGGAAATGAACTGTTCCGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTG‑AAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACTCCTGATTCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCTTAGGCCGGATAAGGCGTAGACGCATCCGGCa                                                                                    <  1:24663/251‑1 (MQ=255)                                                                                                                                                          gtACTGCGGAAGATGCGCAGAAATATCTCGCCATCGCAGATCAAATGTACGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTAGGTGCTTCCAGCCTGATGGCAAGCCTGTTG‑CAAGCGCTGGGCCACGGAGACGGTAAGAGCGCCAGCCGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCCGGCCTACGAGACGTATCTACCCGTCGGCCGGATAAGGCGTAGACGCATCCGGc                                                                                     >  1:160517/1‑250 (MQ=255)                                                                                                                                                                  gAAGATGCGCAGAAATATCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTG‑AAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGCCGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAgccgc                                                                            >  1:118536/1‑251 (MQ=255)                                                                                                                                                                                atatCTCGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTG‑AAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGACGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTc                                                              >  1:227305/1‑251 (MQ=255)                                                                                                                                                                                 tatCTCGCCATCGCAGATGACCTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTG‑ACAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCAGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCCAAAGCCGCCTCATACTCTTTTcc                                                             >  2:192610/1‑251 (MQ=255)                                                                                                                                                                                    cgcgccttcgctgatgatCTGTTTGGCGCTGACGGGGCAGATGCGCGTCACTACCGCTTTGGTGCATCCAGCCTGCTGGCAATCCTTTTG‑AAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCATCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGGTGCGCTAATCAGGCCTAGGAGACGTACCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAGAAGCCGCCGTATACTCGTTTCCTc                                                           <  2:235873/238‑1 (MQ=255)                                                                                                                                                                                      cGCCATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTG‑AAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCggg                                                        >  1:5179/1‑251 (MQ=255)                                                                                                                                                                                        ccATCGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTG‑AAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCggg                                                        >  1:172686/1‑249 (MQ=255)                                                                                                                                                                                                 aTGAACGGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTG‑AAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTg                                             <  2:3138/251‑1 (MQ=255)                                                                                                                                                                                                        gTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTG‑AAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTtctc                                       <  2:88928/250‑1 (MQ=255)                                                                                                                                                                                                                 tGACTGGGCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTG‑AAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGaa                              >  2:4242/1‑250 (MQ=255)                                                                                                                                                                                                                       ggCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTG‑AAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTAc                                                                                                                                   >  2:213740/1‑143 (MQ=255)                                                                                                                                                                                                                       ggCAGATGCGCGTCACTACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTG‑AAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTc                       >  1:103298/1‑251 (MQ=255)                                                                                                                                                                                                                                       tACCGCTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTG‑AAAGCGCTGGGCCACGGTGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGATgg       >  1:254859/1‑251 (MQ=255)                                                                                                                                                                                                                                            cTTTGGTGCTTCCAGCCTGCTGGCAAGCCTGTTG‑AAAGCGCTGGGCCACGGTGACGGTAAGCGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGATGGTCATg  >  2:156781/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      AAATCTCCATCAAAGCGGGTGCGGACTTCATCAAAACCTCTACCGGTAAAGTGGCTGTGAACGCGACGCCGGAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCGGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATTGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTG‑AAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCCTCATACTCTTTTCCTCGGGAGGTTACCTTGTTTCTCGCACAAGAAATTATTCGTAAAAAACGTGATGGTCATG  >  NC_000913/4617789‑4618274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑