Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,617,616	T→C	A98A (GCT→GCC)	deoC →	deoxyribose‑phosphate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,617,616	0	T	C	100.0%	83.9 / NA	26	A98A (GCT→GCC)	deoC	deoxyribose‑phosphate aldolase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/9);  total (17/9)

GACCACCCTGAATGACGACGACACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCTGATGAAGTTGACGTTGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTGAAAGTGATCATCGAAACCGGCGAACTGAAAGACGAAGCGCTGATCCGTAAAGCGTCTGAAATCTCCATCAAAGCGGGTGCGGACTTCATCAAAACCTCTACCGGTAAAGTGGCTGTGAACGCGA  >  NC_000913/4617373‑4617854                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                               gACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATACGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCcgatga                                                                                                                                                                                                                                           >  1:41477/1‑249 (MQ=255)        cgaaaTGACGACAACACCGACGAAAAAGTAATTGCTCTGGGTCATCAGGCAAAACCCCCGGTCGGCAAAACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGt                                                                                                                                                                                                                                  <  1:130736/248‑1 (MQ=255)                                 aagtaattgctcTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATg                                                                                                                                                                                                         >  1:28562/12‑251 (MQ=255)                                      attgctcTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGCAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCggg                                                                                                                                                                                                    >  2:201688/7‑251 (MQ=255)                                                 tcatcaGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGGAACGAGCAgg                                                                                                                                                                                         >  2:267571/1‑251 (MQ=255)                                                               ccccGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTg                                                                                                                                                                           <  1:134937/249‑1 (MQ=255)                                                                                          gTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTg                                                                                                                                                    <  1:103105/247‑1 (MQ=255)                                                                                                   cTCGCTTTATCCCGATTGCTCGCAAAACACTGACAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGGAAAGAAAAGGGTGGTTTTGAACTGGTAAAAGCCTGTTAAGAGGCTTTCGGGGCaacgga                                                                                                                                        >  2:244635/1‑245 (MQ=255)                                                                                                    tCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAAAAGCTTGCGCGGCcgcggagg                                                                                                                                      >  2:269486/1‑247 (MQ=255)                                                                                                      gCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGCATGt                                                                                                                                     >  1:124166/1‑250 (MQ=255)                                                                                                             cccGATTGCTCGCAAAACACTGAAAGAGCAGGGCCCCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTActgctg                                                                                                                              <  1:235736/250‑1 (MQ=255)                                                                                                                gATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACCTCCCACACGGTAACGATGACATCGAAATCGCTCTGGCAGAAACCCGTGCGGCAATCGCCTACGGGGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTTATTGCGGGTAACGAGCAGGTTGGTTTTGACCTGGGGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTGAAAg                                                                                                                          <  1:109881/251‑1 (MQ=255)                                                                                                                       cGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTGAAAGTGatc                                                                                                                     <  1:11693/249‑1 (MQ=255)                                                                                                                             acactGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTGAAAGTGATCATCGAAAc                                                                                                             <  1:85341/248‑1 (MQ=255)                                                                                                                              cactGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTGAAAGTGATCATCGAAAcc                                                                                                            <  1:185852/249‑1 (MQ=255)                                                                                                                                                        aTCCGTATTGCTACGGTAACCAACTTCCCAAACGGTAACGATGACCTAGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGACTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGACACGAGCAGGTTGGTCTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCCGCGGATGTACTGCTGAAAGGGCTCATCGAAACCGGCGAACTGAAAAACGAAGCGCTGAt                                                                                  >  1:237643/1‑251 (MQ=255)                                                                                                                                                          ccGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGGTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTAATGGTGAAAGTGATCATAGAAAACGGCGAACTTAAAGACGAAGCGCGGATcc                                                                                >  2:222595/1‑251 (MQ=255)                                                                                                                                                            gTATTGCTACGGTAACCAAATTCCCACGCGGTAACGATGGCATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGATCAGGTTGGTTTTTACCTGGGGAAAGCCTGGAAAGGGGATTGGGCGGCCGCGAAGGTACTGATGGAAGTGTTCATAGAAAACGGGGAACTGAAAAAAGAAGCGCTGagtcgt                                                                              >  2:96969/1‑246 (MQ=255)                                                                                                                                                                       gTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGACACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTGAAAGTGGTCATCGAAACCGGCGAAATGAAAGGAGAAGCGCTGATCCGTAAAGCGTCTg                                                                    >  1:170452/1‑250 (MQ=255)                                                                                                                                                                                   ccACACGGTAACGCTGACATCGAAATCGCGCTGGCAGAAACCAGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGAAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTGAAAGTGATCATCCAAACCGGCCAACTGAAAGACGAAGCGCTGATCCGTAAAGCGTCTGAAATATACATCa                                                        >  1:55223/1‑250 (MQ=255)                                                                                                                                                                                         ggTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAAAGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTGAAAGTGAACATCGAAACCGGCGAACTGAAAGAAGAAGGGCTGATCCGTAAAGCGTCTGAAATCTCCAACAAAGCggg                                                 >  1:176121/1‑251 (MQ=255)                                                                                                                                                                                               gatgacatcgaaatcGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTGAAAGTGATCATCGAAACCGGCGAACTGAAAGACGAAGCGCCGATCCGTAAAGCGTCTGAAATCTCCATCAAAGCGGGGGCggg                                           >  1:148676/4‑250 (MQ=255)                                                                                                                                                                                                         aaatcgCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTGAAAGGGATCATCGAAACCGGCGGACTGAAAGACGAAGCGCTGATCCGTAAAGCGTCTGAAATCTCCATCAAAGCGGGGGCGGACTTCATCaa                                  >  1:3138/3‑250 (MQ=255)                                                                                                                                                                                                           atcgCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTGAAAGTGATCATCGCAACCGGCGAACCGAAAGAAGAAGCGCTGATCCGTAAAGCGTCTGAAATCTCCATCAAAGCGGGGGCGGACTTCCTCAAAAc                               >  1:257429/1‑251 (MQ=255)                                                                                                                                                                                                                gcTGGCAGCAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGGAACGAGCAGGTTGGTTTTGACCTGGTGGAAGCCTGGAAAGAGGCTTGCGCGGCCGCGAATGTACGGCTGAAAAGGATCAACGGAACCGGCGACCGGGAAGACGAAACGCTGATCCGTAAAGCGTCTGAAATCTCCATCAAACCGGGGGCGGTCTTCCTCAAAAcccct                           >  2:11707/1‑247 (MQ=255)                                                                                                                                                                                                                                        cGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTGAAAGTGATCATCGAAACCGGCGAACTGAAAGACGAAGCGCTGATCCGTAAAGCGTCTGAAATCTCCATCAAAGCGGGTGCGGACTTCATCAAAACCTCTACCGGTAAAGTGGCTGTGAACGCGa  <  1:267571/251‑1 (MQ=255)                                                                                                                                                                                                                                        cGCCTACGGTGACGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGGCCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTGGAAGTGATCCTCGAAAACGGCGAACTGAAAGACGAAGCGCTGATCCGTAAACCGTCTGAAAACTCCATCCAAGCGGGTGCGGACTTCATCAAAACCTCTACCGGTAAAGAGGCTGGGAAcgcg   >  1:64804/1‑250 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                               GACCACCCTGAATGACGACGACACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCTGATGAAGTTGACGTTGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTGAAAGTGATCATCGAAACCGGCGAACTGAAAGACGAAGCGCTGATCCGTAAAGCGTCTGAAATCTCCATCAAAGCGGGTGCGGACTTCATCAAAACCTCTACCGGTAAAGTGGCTGTGAACGCGA  >  NC_000913/4617373‑4617854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑