Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,617,499	T→A	T59T (ACT→ACA)	deoC →	deoxyribose‑phosphate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,617,499	0	T	A	100.0%	94.9 / NA	31	T59T (ACT→ACA)	deoC	deoxyribose‑phosphate aldolase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (18/13);  total (18/13)

GATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCTGATGAAGTTGACGTTGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTGAAAGTGATC  >  NC_000913/4617261‑4617739                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                 gATGTTAGAATACTAACAAACTCGCAAGGTGATTTTTATTGGCGACAAGCCAGGAGCATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGc                                                                                                                                                                                                                                        >  2:185852/1‑249 (MQ=255)                 aaaaCTCGAAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATTACTGATCTGAAAGCAATAAGCCTGCGTGCACTGAAATTGATGGACCTGACCCCCCTGAATGACGACGACCCCGACGAGAAAGTAATTGTTTTGTGTCATCGGGCCAAACCCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATcc                                                                                                                                                                                                                      <  1:21014/250‑1 (MQ=255)                           aGGTGAATTTTCTTGGCGACACGCCAGGAGAATGAAATGACGGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGCCACCGACGAGAATGTAATTGATCTGTGTCATCAGGCACAAACCCCGGTCGACAATACCGCCGCTATCTGTATCAATACTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGCCAATCCGTAttgatac                                                                                                                                                                                                            >  2:197886/1‑245 (MQ=255)                           aGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTCAAAGAAAGCAGCCTGCGTGCACTGCAATTGATGGACCTGACCAACCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTGTCATAAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAACGAGCAGGGCACCCCGGAAATCCGTATTGCTAc                                                                                                                                                                                                            >  1:172401/1‑251 (MQ=255)                                               aaGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACg                                                                                                                                                                                        >  2:134937/1‑251 (MQ=255)                                                   cAGGAGAATGAAATGACTGATCTAAAAGCAAGCAGCCGGCGTGCACTAAAATTGATGGACCTGACCCCCCTAAATGACGACGACACCGACGAAAAAGTAATTCCTCTGTGTCATCAGGCCAAACCCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTaa                                                                                                                                                                                    <  1:23897/251‑1 (MQ=255)                                                          atgaaatgaCTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTGTCATCAGACCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCCCCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACgatgac                                                                                                                                                                             >  1:130476/1‑251 (MQ=255)                                                                     aatcggaaagcaaaaggcatGCGGGCACTGAAATTGATGGACCTGCCCCCCCTAAAAGACGCCACCCCCGACAAGAAAGTAATGGCTCTGTGTCAACAGGCAAAACCCCCGGCCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACATCCCACACGGTAACGATgacatcgaaatc                                                                                                                                                                    <  2:136003/230‑1 (MQ=255)                                                                           aaaGCAACAAGCCTGCGTGCCGGGAAATTGATGGACCTGCCCCCCCTGAATGACAACGACACCGCCGAGAAAGTAATTGCTCTGTTTCACCAGCCCAAACCCCCGGCCGGAAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGc                                                                                                                                                            <  2:15109/251‑1 (MQ=255)                                                                                  gcagcCTGCGTGCACTGAAATTGCTGGACCTGACCCCCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTCTCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCACAACACTGAAAGCGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGCCATCGCCATCGCGCTTGCAGAAAcc                                                                                                                                                     >  1:12427/1‑251 (MQ=255)                                                                                        tGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGCACCGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCg                                                                                                                                               >  1:226463/1‑251 (MQ=255)                                                                                              cACTGAAAAGGATGGACCGGCCAACCCGGAATGCCGACGACACCGACTAGAAAGTAATTGCTCGGTGCCATCAGGCCAACTCCCGGGGCGCCAAAACCGCCGCTATCTGTATCTATCCGCGCGTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACAGCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATc                                                                                                                                         <  2:12427/251‑1 (MQ=255)                                                                                                            gACCTGACCCCCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTGTCATCAGGCCAAACCCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGaaa                                                                                                                                                       <  1:223821/223‑1 (MQ=255)                                                                                                            gACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGaaa                                                                                                                                                       >  2:223821/1‑223 (MQ=255)                                                                                                            gACCTGACAACCCTGAATGACGACGACACCGAAGAGAAAGTAATTGCTCTGTGTCATCAGGCCAAAACCCCGGCCGGCAATCCCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGACAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAAACAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGccgg                                                                                                                           >  2:163162/1‑248 (MQ=255)                                                                                                                 gACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCTCTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATACGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCcgatga                                                                                                                        >  1:41477/1‑249 (MQ=255)                                                                                                                        cgaaaTGACGACAACACCGACGAAAAAGTAATTGCTCTGGGTCATCAGGCAAAACCCCCGGTCGGCAAAACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGt                                                                                                               <  1:130736/248‑1 (MQ=255)                                                                                                                                                 aagtaattgctcTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATg                                                                                      >  1:28562/12‑251 (MQ=255)                                                                                                                                                      attgctcTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGCAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCggg                                                                                 >  2:201688/7‑251 (MQ=255)                                                                                                                                                              gtgtCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGcc                                                                                                                             >  1:65456/1‑198 (MQ=255)                                                                                                                                                                 tcatcaGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGGAACGAGCAgg                                                                      >  2:267571/1‑251 (MQ=255)                                                                                                                                                                               ccccGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTg                                                        <  1:134937/249‑1 (MQ=255)                                                                                                                                                                                                          gTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTg                                 <  1:103105/247‑1 (MQ=255)                                                                                                                                                                                                                   cTCGCTTTATCCCGATTGCTCGCAAAACACTGACAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGGAAAGAAAAGGGTGGTTTTGAACTGGTAAAAGCCTGTTAAGAGGCTTTCGGGGCaacgga                     >  2:244635/1‑245 (MQ=255)                                                                                                                                                                                                                    tCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAAAAGCTTGCGCGGCcgcggagg                   >  2:269486/1‑247 (MQ=255)                                                                                                                                                                                                                      gCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGCATGt                  >  1:124166/1‑250 (MQ=255)                                                                                                                                                                                                                             cccGATTGCTCGCAAAACACTGAAAGAGCAGGGCCCCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTActgctg           <  1:235736/250‑1 (MQ=255)                                                                                                                                                                                                                                gATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACCTCCCACACGGTAACGATGACATCGAAATCGCTCTGGCAGAAACCCGTGCGGCAATCGCCTACGGGGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTTATTGCGGGTAACGAGCAGGTTGGTTTTGACCTGGGGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTGAAAg       <  1:109881/251‑1 (MQ=255)                                                                                                                                                                                                                                       cGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGTGGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTGAAAGTGatc  <  1:11693/249‑1 (MQ=255)                                                                                                                                                                                                                                           aaaCACTGAAAGAGCAGGGCACCCCGGAAATCCGTCTTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGc                                                                                                                              >  2:29344/1‑121 (MQ=255)                                                                                                                                                                                                                                           aaaCACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGc                                                                                                                              <  1:29344/121‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                 GATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCTGATGAAGTTGACGTTGTGTTCCCGTACCGCGCGCTGATGGCGGGTAACGAGCAGGTTGGTTTTGACCTGGTGAAAGCCTGTAAAGAGGCTTGCGCGGCAGCGAATGTACTGCTGAAAGTGATC  >  NC_000913/4617261‑4617739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑