| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,617,409 | G→A | V29V (GTG→GTA) | deoC → | deoxyribose‑phosphate aldolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,617,409 | 0 | G | A | 96.0% | 67.8 / ‑3.1 | 25 | coding (87/780 nt) | deoC | deoxyribose‑phosphate aldolase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (15/9); minor base . (0/1); total (15/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.63e-01 | |||||||||||
AATTATTTGAACCAGATCGCATTACAGTGATGCAAACTTGTAAGTAGATTTCCTTAATTGTGATGTGTATCGAAGTGTGTTGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTGATCGCC‑CTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCTGATGAAGTTGACGT > NC_000913/4617172‑4617630 | aaTTATTTGAACCAGATCGCATTACAGTGATGCAAACTTGTAAGTAGATTTCCTTAATTGTGATGTATATCGAAGTGTGTTGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCT‑CTGTGtc < 2:26497/251‑1 (MQ=255) cATTACAGTGATGCAAACTTGTAAGTAGATTTCCTTAATTGTGATGTATATCGAAGTGTGTTGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACCCCGACGAGAAAGTAATTGCT‑CTGTGTCATCAGGCCAAAACCCCGGt > 1:136003/1‑251 (MQ=255) ttGTACGTAGATATCCTTCATTGTGCCGTATCTCGCAGTGTGTTGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGCATTTTATTGCCGACAAGCCAGGAGAATGAAACGCCTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATCGATGGACCTGACCACCCTGAATGACGCCGACACCGACGAGAAAGTAATTGCT‑CTATGTCATCACGCCCAAACCCCGGTCGGCACTACCGCCGCTAt > 2:109881/1‑251 (MQ=255) tGTAAGTAGATTTCCTTAATTGTGATGTATATCGAAGTGTGTTGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCT‑CTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATc > 2:23897/1‑251 (MQ=255) tAAGTAGATTTCCTTAATTGTGATGTATATCGAAGTGTGTTGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCT‑CTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTg > 2:103105/1‑251 (MQ=255) gaaggtaaaTCGAAGTGGGTTGCGGTGAAGAATTGAGAAAAATAAAAAACTCGAAAGGTGATTTTGATGGGCGAAAAGCCAGGAAAATGAAATTACTGATCTAAAAGCAAACAGCCTGCGTGCACTGAAATTGATGGACCTGACCCCCCTAAAGAACGACGACACCGACGAGAAAGTAATTGCT‑CTGTGTCATCAGGCCAAAACCCCGGCCGGAAATACCGCCGCTATCTGTATCTATCCTCGCTTTATcc < 1:185664/243‑1 (MQ=255) aTGTATATCGAAGTGTGTTGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCT‑CTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCg > 1:176271/1‑251 (MQ=255) ggtgtgtTGCGGAGTAGATGTTAAAAAACTAACAAACTCGCAAGTTAAATTTTATTGGCGACAAGCCAGGAGAATAAAATGACTAATCTGAAAGCAAGCAGCCGGCGTGCATTGAAATTGAGGGACCGGCCCACCCTAAATGACGCCGACACCGACGAGAAAGT‑ATTTCCTCTGTTTCATCAGGCCAAACCCCCGTTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCaa < 1:114402/250‑1 (MQ=255) gATGTTAGAATACTAACAAACTCGCAAGGTGATTTTTATTGGCGACAAGCCAGGAGCATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCT‑CTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGc > 2:185852/1‑249 (MQ=255) aaaaCTCGAAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATTACTGATCTGAAAGCAATAAGCCTGCGTGCACTGAAATTGATGGACCTGACCCCCCTGAATGACGACGACCCCGACGAGAAAGTAATTGTT‑TTGTGTCATCGGGCCAAACCCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATcc < 1:21014/250‑1 (MQ=255) aGGTGAATTTTCTTGGCGACACGCCAGGAGAATGAAATGACGGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGCCACCGACGAGAATGTAATTGAT‑CTGTGTCATCAGGCACAAACCCCGGTCGACAATACCGCCGCTATCTGTATCAATACTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGCCAATCCGTAttgatac > 2:197886/1‑245 (MQ=255) aGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTCAAAGAAAGCAGCCTGCGTGCACTGCAATTGATGGACCTGACCAACCTGAATGACGACGACACCGACGAGAAAGTAATTGCT‑CTGTGTCATAAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAACGAGCAGGGCACCCCGGAAATCCGTATTGCTAc > 1:172401/1‑251 (MQ=255) aaGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCT‑CTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACg > 2:134937/1‑251 (MQ=255) cAGGAGAATGAAATGACTGATCTAAAAGCAAGCAGCCGGCGTGCACTAAAATTGATGGACCTGACCCCCCTAAATGACGACGACACCGACGAAAAAGTAATTCCT‑CTGTGTCATCAGGCCAAACCCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTaa < 1:23897/251‑1 (MQ=255) atgaaatgaCTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCT‑CTGTGTCATCAGACCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCCCCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACgatgac > 1:130476/1‑251 (MQ=255) aatcggaaagcaaaaggcatGCGGGCACTGAAATTGATGGACCTGCCCCCCCTAAAAGACGCCACCCCCGACAAGAAAGTAATGGCT‑CTGTGTCAACAGGCAAAACCCCCGGCCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACATCCCACACGGTAACGATgacatcgaaatc < 2:136003/230‑1 (MQ=255) aaaGCAACAAGCCTGCGTGCCGGGAAATTGATGGACCTGCCCCCCCTGAATGACAACGACACCGCCGAGAAAGTAATTGCT‑CTGTTTCACCAGCCCAAACCCCCGGCCGGAAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGc < 2:15109/251‑1 (MQ=255) gcagcCTGCGTGCACTGAAATTGCTGGACCTGACCCCCCTGAATGACGACGACACCGACGAGAAAGTAATTGCT‑CTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTCTCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCACAACACTGAAAGCGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGCCATCGCCATCGCGCTTGCAGAAAcc > 1:12427/1‑251 (MQ=255) tGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCT‑CTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGCACCGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCg > 1:226463/1‑251 (MQ=255) cACTGAAAAGGATGGACCGGCCAACCCGGAATGCCGACGACACCGACTAGAAAGTAATTGCT‑CGGTGCCATCAGGCCAACTCCCGGGGCGCCAAAACCGCCGCTATCTGTATCTATCCGCGCGTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACAGCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATc < 2:12427/251‑1 (MQ=255) gACCTGACCCCCCTGAATGACGACGACACCGACGAGAAAGTAATTGCT‑CTGTGTCATCAGGCCAAACCCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGaaa < 1:223821/223‑1 (MQ=255) gACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCT‑CTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGaaa > 2:223821/1‑223 (MQ=255) gACCTGACAACCCTGAATGACGACGACACCGAAGAGAAAGTAATTGCT‑CTGTGTCATCAGGCCAAAACCCCGGCCGGCAATCCCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGACAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAAACAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGccgg > 2:163162/1‑248 (MQ=255) gACCACCCTGAATGACGACGACACCGACGAGAAAGTAATTGCT‑CTGTGTCATCAGGCCAAAACCCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATACGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCcgatga > 1:41477/1‑249 (MQ=255) cgaaaTGACGACAACACCGACGAAAAAGTAATTGCT‑CTGGGTCATCAGGCAAAACCCCCGGTCGGCAAAACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACACTGAAAGAGCAGGGCACCCCGGAAATCCGTATTGCTACGGTAACCAACTTCCCACACGGTAACGATGACATCGAAATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCCGATGAAGTTGACGt < 1:130736/248‑1 (MQ=255) | AATTATTTGAACCAGATCGCATTACAGTGATGCAAACTTGTAAGTAGATTTCCTTAATTGTGATGTGTATCGAAGTGTGTTGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTGATCGCC‑CTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTAACGACGACATCGACATCGCGCTGGCAGAAACCCGTGCGGCAATCGCCTACGGTGCTGATGAAGTTGACGT > NC_000913/4617172‑4617630 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |