Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	5,642	A→C	intergenic (+112/+41)	yaaX → / ← yaaA	DUF2502 domain‑containing protein YaaX/DNA binding and peroxide stress response protein YaaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	5,642	0	A	C	100.0%	56.2 / NA	22	intergenic (+112/+41)	yaaX/yaaA	DUF2502 domain‑containing protein YaaX/DNA binding and peroxide stress response protein YaaA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (11/11);  total (11/11)

GAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAATCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCG  >  NC_000913/5404‑5839                                                                                                                                                                                                                                               |                                                                                                                                                                                                      gAAACAACATTATGAATGGCGAGGCCATCGCTGGCACCCACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCCTGGTCCAAGAAAACATCCCCCCTAAATGACAAATGCCGGGTAACAATCCGGCATTTACCGCCTGGTGCGACGCTGGCGCGTCTTATCAAGCCTACGTGACTTCTGCAATATATTGAATCTGCATGCTTTTTTAGGCCGGATAAGGGGtt                                                                                                                                                                                           >  2:161415/1‑251 (MQ=255)                    cGAGGCAATCGCTGGCACCCACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGGCCAGGCAAACATCACCGCTAAATGACAAATTCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTGAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTg                                                                                                                                                                        >  1:134359/1‑251 (MQ=255)                     gAGGCAATCGCTGGCACCCACCCGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTGAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTGa                                                                                                                                                                       <  1:147697/251‑1 (MQ=255)                                tGGCACCCACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGGCATGGTCCCGGCAAAAATTACCGCTAAATGACAAATGACGGGTAAAAAACAGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCCTATCAGGCCTACGTGGATTCTGAAATATATTGAATATGCATGCTTTTGTAGGGCGGGTAAGGCGTTCAAGCCGCATCCGGCATTGACTGCCAACtt                                                                                                                                                             >  1:259537/1‑250 (MQ=255)                                   cccccacacGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTGAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACg                                                                                                                                                         <  1:256991/246‑1 (MQ=255)                                         cccGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTGAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTc                                                                                                                                                   <  1:81501/249‑1 (MQ=255)                                            ggcccgccgCCACCGCCGCGCCACCATAAGAAAGCTCCACATGAACATCACGGCGGTCATGGTCCAGGCAAACTTCACCGCTAAATGACAAATGCCGGGAAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTGAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGTAAACTTAACGCTGCTCGTa                                                                                                                                                <  2:76910/248‑1 (MQ=38)                                             gACCGCCGCAACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGGCATGGTCCAGGCCAACATCACCGCCAAAAGACCAATGCCGGGGAACAATCCGGCATTAAACGCCTGATGCGACGCTGGCGCGTCTTATCAGGTCTAAGTGGATTCTGCAATATATTGAATCTGCATTCTTTTGTAGGCCGGATAAGGCGGTCACGCCGCCTCCGGCCTTGACTGCAAACTTAACGCTGATCGt                                                                                                                                                 >  1:80655/1‑249 (MQ=255)                                                      cACCGCCGCGCCACCATAAGCAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACCTCACCGCTAAATGACAAATGCCGGGGAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAAGCCTACGTGAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGGCGGGTAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAac                                                                                                                                      >  1:59419/1‑251 (MQ=255)                                                                   ccaTAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACCTCACCGCTAAATGAACAATGCCCGGCAACAATCCGGCATTCAGCGCCCGATGCGACGCTGGCGCGTCTTATCAAGCCTAAGTGAATTCTGCAATATATTGAATCTGCATGCTTTTTTAGGCCGGATAAGGCGTTCCCGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCAt                                                                                                                         >  1:128001/1‑251 (MQ=255)                                                                          aacgCTCATCATGATCAGCACGGCGGTCATGGTCCAGGCAAACAGCACCGCTAAATGACAAATGACGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTGAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCCGGATAAGGCGTTCCCGCCGCATACGGCATTGACTGCAAAATTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTgga                                                                                                                  <  2:3536/248‑1 (MQ=255)                                                                                            caCGGCGGTCATGGTGCAGGGCACCATCACCGCTAAATGACAAATGCCCGGTAACAATCCGGCATTTAGCGCCCGCTGCGACGCTGGCGCGTCTTATCAGGCCTACGAGAATTCTGCCATATATTGAATCTGCCTGCTTTTGTCGGCCGGGTAAAGCGTTCACGCCGCATCCCGCATTGACTGCAAACTTTAAGCTGCTCGGAGCGTTTAAACACCAGTTCGCCCTTCCTGGAGGGAGCTTCCTCAaaga                                                                                                 >  2:94992/1‑250 (MQ=255)                                                                                                   gTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTGAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACgct                                                                                                                                                       >  1:7601/1‑189 (MQ=255)                                                                                                   gTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTGAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACgct                                                                                                                                                       <  2:7601/189‑1 (MQ=255)                                                                                                         gTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTACCAGGCCTACGTGAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAAGCTTCATCAAAGAAGTAACCTTCGCTa                                                                                   <  2:59419/251‑1 (MQ=255)                                                                                                                         ccGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTGAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAAGCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGtt                                                                   >  1:1740/1‑251 (MQ=255)                                                                                                                              aaaTGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTGAATTCTGCAATATATTGTATCTGCATGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAAGCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGctc                                                               <  2:1473/250‑1 (MQ=255)                                                                                                                               aaTGACAAATGCCGGGTAACAATCCGGCAGTCAGCGCTGGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTGAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAATCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTg                                                             <  1:6087/251‑1 (MQ=255)                                                                                                                                                        ggCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTGAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAAGCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCaataat                                    >  2:90933/1‑251 (MQ=255)                                                                                                                                                              cAGCGCCTGATGCGACGCGGGCGCGTCTTATCAGGCCTACGTGAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAAGCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATAAAAc                               <  1:126377/250‑1 (MQ=255)                                                                                                                                                                                      gTCTTATCAGGCCTACGTGAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAAGCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATAAAACGACTCATCAGACCGCGTGCTTTCtt      >  2:142485/1‑251 (MQ=255)                                                                                                                                                                                           aTCAGGCCTACGTGAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAAGCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATAAAACGACTCATCAGACCGCGTGCTTTCTTAGCg  <  1:46379/250‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                      GAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAATCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCG  >  NC_000913/5404‑5839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑