Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,602,089	C→T	V3V (GTG→GTA)  *257* (TGA→TAA)	yjjB ← yjjP ←	putative succinate exporter YjjB putative succinate exporter YjjP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,602,089	0	C	T	100.0%	131.5 / NA	42	V3V (GTG→GTA)  *257* (TGA→TAA)	yjjB yjjP	putative succinate exporter YjjB putative succinate exporter YjjP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/17);  total (25/17)

GGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCGACCAGCATAGAAGCCATAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGACCGCAGGAATGGCGGCGAGGATCATATCCTGCGCCAACGCTAACAGAAATTCGATCACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGCCGACGCAGGTAGCCAGTGTCAGCAGACTGGCGATCGCCCAGCGTGCCAGTCCGGTATTGATGTGGCCTTTAAACATATCGGCGACGGCATTAATCAACGGAAAGCCCGGCACTAGCAGCAGAACGCTGGCGGCCATCGCAATGGTGGGGGTATTGCTGAAAGTGGGGAGTTGCAAAAGCAATCCGGAAATGGTGGTGG  >  NC_000913/4601844‑4602332                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    ggATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCGACCAGCATAGAAGCCATAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGACCGCAGGAATGGCGGCGAGGATCATATCCTGCGCCAACGCTAACAGAAATTCGATTACAcc                                                                                                                                                                                                                                                >  1:161686/1‑251 (MQ=255)              aCCAGCGCGACCATTGACTACCAATGGTACCGAACAGCATAGAAGCCATAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACCCCAGCGTAAAGCCCGCACGGGAACGTTGAACAACATCGCAAAGCCGACCGCCGGAAGGGCGGCGAGGATCATATCCTGCGCCACCGCTAACAGAAATTCGATTACACCCATCCGCGCAGcc                                                                                                                                                                                                                                   >  2:243722/1‑251 (MQ=255)                       aCCATTGAATACCAATGGTACCGACCAGCATAGAAGCCATAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGACCGCAGGAATGGCGGCGAGGATCATATCCTGCGCCAACGCTAACAGAAATTCGATTACACCCATCCGCGTAGCCCCCAAATCg                                                                                                                                                                                                                          >  1:57958/1‑251 (MQ=255)                                       ggTACCGACCAGCATAGAAGCCATAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGACCGCAGGAATGGCGGCGAGGATCATATCCTGCGCCCACGCTAACAGAAATTCGATTACAACCATCCGCGTAGCCCCCAAATCGTCCATGCCATCACtcc                                                                                                                                                                                                          >  1:228139/1‑249 (MQ=255)                                          aCCGACCAGCATAGAAGCCATAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCTAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGACCGCAGGAATGGCGGCGAGGATCATATCCTGCGCCAACGCTAACAGAAATTCGATTACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGcc                                                                                                                                                                                                       <  2:237707/251‑1 (MQ=255)                                                          gCCATAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGACCGCAGGAATGGCGGCGAGGATCATATCCTGCGCCAACGCTAACAGAAATTCGATTACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGCCGACGCAGGTAGCCAgt                                                                                                                                                                                       <  1:250676/251‑1 (MQ=255)                                                               aaaGGTGGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGACCGCAGGAATGGCGGCGAGGATCATATCCTGCGCCAACGCTAACAGAAATTCGATTACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGCCGACGCAGGTAGCCAGTGTca                                                                                                                                                                                   <  1:66397/250‑1 (MQ=255)                                                                aaGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGACCGCAGGAATGGCGGCGAGGATCATATCCTGCGCCAACGCTAACAGAAATTCGATTACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGCCGACGCAGGTAGCCAGTGTcagc                                                                                                                                                                                 >  1:15020/1‑251 (MQ=255)                                                                                    cAACCCGCTGGTCATCCAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGACCGCAGGAATGGCGGCGAGGATCATAACCTGCGCCAACGCTAACAGAAATTCGATTACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGCCGACGCAGGTAGCCAGAGTCAGCAGACTGGCGATCGCCCAGCg                                                                                                                                                             >  2:233076/1‑251 (MQ=255)                                                                                       cccGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAAAACCATCGCAAAGCCGACCGCAGGAATGGCGGCGAGGATAATATCCTGCGCCAACGCTAACAGAAATTAGATTACACCCCTCCGCGTAGCCCCCAAATCGTCAGTGCCATCACACCGCCGACGCCGGTAGCCAGCGTCCGCAGACAGGCGCTCGACCAGCGTGc                                                                                                                                                          >  2:64379/1‑251 (MQ=255)                                                                                         cGCTGGTCATCAAGAGCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGACCGCAGGAATGGCGGCGAGGATCATATCCTGCGCCAACGCTAACAGAAATTCGATTACACC‑ATCCGCGGAGCCCCCAAATCGTCAGTGCCATCACTACGCCGACGCAGGTAGCCAGTGTCAGCAGACTGGCGATCGCCCAGCGTGCCAg                                                                                                                                                       <  2:161686/251‑1 (MQ=255)                                                                                              gTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGACCGCAGGAATGGCGGCGAGGATCATATCCTGCGCCAACGCTAACAGAAATTCGATTACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCAGCACTACGCCGACGCAGGTa                                                                                                                                                                                             <  1:261518/209‑1 (MQ=255)                                                                                              gTCATCAAGATCATTCGGGAACCATGACATATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGACCGCAGGCATGGCGGCGAGGCGCATATCCTGCGCCCACGCTAACAGAAATTCGATTACACCCATCCGCGTAGCCCCCAAATCGACAGTGCCATCACTACGCCGACGCAGGTa                                                                                                                                                                                             >  2:261518/1‑209 (MQ=255)                                                                                               tcatcaAGATCATTCGGGAACCCTGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGACCGCAGGAATGGCGGCGAGGATCATATCCTGCGCCAACGCTAACAGAAATTCGATTACACCCATCCGCGTAGCCCCCAAATCGTCCGTGCCATCACTACGCCGAc                                                                                                                                                                                                    >  2:72694/1‑201 (MQ=255)                                                                                               tcatcaAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGACCGCAGGAATGGCGGCGAGGATCATATCCTGCGCCAACGCTAACAGAAATTCGATTACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGCCGAc                                                                                                                                                                                                    <  1:72694/201‑1 (MQ=255)                                                                                                         cATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCACGCACGGGAACGTTGAACACCATCGCAAAGCCGACCGCAGGAATGGCGGCGAGGATCATATCCTGCGCCAACGCTACCAGAAATTCGATTACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGCCGACGCAGGTAGCCAGTGTCAGCAGACTGGCGATCGCCCAGAGGGCCAGTCCGGCATTGAtgtg                                                                                                                                        >  1:158816/1‑251 (MQ=255)                                                                                                          aTTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGCCCGCAGGAATGGCGGCGAGGATCATATCCTGCGCCAACGCTAACAGAAATTCGATTACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGCCGACGCAGGTAGCCAGTGTCAGCAGACTGGCGATCGCCCAGCGCGCCAGTCCgggatggctgtgg                                                                                                                                       >  2:191711/1‑240 (MQ=255)                                                                                                          aTTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGACCGCAGGAATGGCGGCGAGGATCATATCCTGCGCCAACGCTAACAGAAATTCGATTACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGCCGACGCAGGTAGCCAGTGTCAGCAGACTGGCGATCGCCCAGCGTGCCAGTCCGGTATTGATGTgg                                                                                                                                       <  1:8988/251‑1 (MQ=255)                                                                                                               ggAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGACCGCAGGAATGGCGGCGAGGATCATATCCTGCGCCAACGCTAACAGAAATTCGATTACACCCATCCGCGTAGCCCCCAAATCGTCAGTTCCATCACTACGCCGACGCAGGTAGCCAGTGTCAGCAGACTGGCGATCGCCCAGCATGCCAGTCCGGTATTGGTGTGGCCttt                                                                                                                                  >  2:216626/1‑251 (MQ=255)                                                                                                                      tGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGACCGCAGGAATGGCGGCGAGGATCATATCCTGCGCCAACGCTAACAGAAATTCGATTACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGCCGACGCAGGTAGCCAGTGTCAGCAGACTGGCGATCGCCCAGCGTGCCAGTCCGGTATTGATGTGGCCTTTAAACat                                                                                                                            >  1:119754/1‑250 (MQ=255)                                                                                                                      tGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGACCGCAGGAATGGCGGCGAGGATCATATCCTGCGCCAACGCTAACAGAAATTCGATTACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGCCGACGCAGGTAGCCAGTGTCAGCAGACTGGCGATCGCCCAGCGTGCCAGTCCGGTATTGATGTGGCCTTTAAACat                                                                                                                            >  1:265158/1‑250 (MQ=255)                                                                                                                          cTATCGAGCCAAGCAGCGCCAACCAGAGTAACGCCCGCACGGGAACGTTGAACACCATAGCAAAGCCGAACGCAGGAATGGCGGCGAGGATCATATCCTGCGCCAAAGCTAACAGAAATTCGATTAAACCCATCCGCGTAGCCCCCGAATCGTCAGTGCCATTACTACGCCGACGCAGGTAACCAGGGTCAGCAGACTGGCGATAGCCACGCCTGCCAGTCCGGTATTGATGTGCACTTTAAACATATCgg                                                                                                                       >  2:117206/1‑251 (MQ=255)                                                                                                                              cGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGACCGCAGGAATGGCGGCGAGGATCATATCCTGCGCCAACGCTAACAGAAATTCGATTACACCCATCCGCGTAGCCCACAAATCGTCAGTGCCATCACTACGCCGACGCAGGTCGCCAGTGTCAGCAGACTGGCGCTCGCCCAGCGTGCCCGTCCGGTATTGCTGTGGCCTTTAAACATATCGGCGAc                                                                                                                   >  1:149009/1‑251 (MQ=255)                                                                                                                                    aaGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGACCGCAGGAATGGCGGCGAGGATCATATCCTGCGCCAACGCTAACAGAAATTCGATTACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGCCGACGCAGGTAGCCAGTGTCAGCAGACTGGCGATCGCCCAGCGTGCCAGTCCGGTATTGATGTGGCCTTTAAACATATCGGCGACGGCAtt                                                                                                             <  1:91200/251‑1 (MQ=255)                                                                                                                                     agcagcGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGACCTCAGGAATGGCGGCGAGGATCATATCCTGCGCCAACGCTAACAGAAATTCGATTACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGCCGACGCAGGTAGCCAGTGTCAGCAGACTGGCGATCGCCCAGCGTGCCAGTCCGGTATTGATGTGGCCTTTAAACATATCGGCGACGGCATTa                                                                                                            <  2:229678/251‑1 (MQ=255)                                                                                                                                     agcagcGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGACCGCAGGAATGGCGGCGAGGATCATATCCTGCGCCAACGCTAACAGAAATTCGATTACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGCCGACGCAGGTAGCCAGTGTCAGCAGACTGGCGATCGCCCAGCGTGCCAGTCCGGTATTGATGTGGCCTTTAAACATATCGGCGACGGCAtt                                                                                                             >  1:183130/1‑250 (MQ=255)                                                                                                                                            cacaCCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGACCGCAGGAATGGCGGCGAGGATCATATCCTGCGCCAACGCTAACAGAAACTCGATTACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGCCGACGCAGGTAGCCAGTGTCAGCAGACTGGCGATCGCCCAGCGTGCCAGTCCGGTATTGATGTGGCCTTTAAACATATCGGCGACGGCATTAATCAACg                                                                                                     >  1:184466/1‑251 (MQ=255)                                                                                                                                              caccacagTAACGACCGCACGGGAACGTTGAACACCAACGCAAAGCCGACCGCAGGAATGGCGGCGAGGATCATATCCTGCGCCAACGCTAACAGCAATTCGATTACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGCCGACGCAGGTAGCCAGTGTCAGCAGCCTGGCGATCGCCCAGCGTGCCAGTCCGGGATTGATGTGGCCTTTAACCATATCGGCGACGGCATTAATCCACggg                                                                                                   >  1:213137/8‑250 (MQ=255)                                                                                                                                                  aGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGACCGCAGGAATGGCGGCGAGGATCATATCCTGCGCCAACGCTAACAGAAATTCGATTACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGCCGACGCAGGTAGCCAGTGTCAGCAGACTGGCGATCGCCCCGCGTGCCAGTCCGGTATTGATGTGGCCTTTAAACCTATCGGCCACGGCATTAATCAACGGAAAGc                                                                                               >  2:135789/1‑251 (MQ=255)                                                                                                                                                     gTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGACCGCAGGAATGGCGGCGAGGATCATATCCTGCGCCAACGCTAACAGAAATTCGATTACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGCCGACGCAGGTAGCCAGTGTCAGCAGACTGGCGATCGCCCAGCGTGCCAGTCCGGGATTGATGTGGCCTTTAAACATATCGGCGACGGCATTAATCAACGGAAAGCCCg                                                                                            >  2:153132/1‑251 (MQ=255)                                                                                                                                                                        gTTGAACACCATCGCAAAGCCGACCGCAGGAATGGCGGCGAGGATCATATCCTGCGCCAACGCTAACAGAAATTCGATTACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGCCGACGCAGGTAGCCAGTGTCAGCAGACTGGCGATCGCCCAGCGTGCCAGTCCGGGATTGATGTGGCCTTTAAACATATCGGCGACGGCATTAATCAACGGAAAGCCCGGCACTAGCAGCAGCACGcg                                                                         >  2:78612/1‑250 (MQ=255)                                                                                                                                                                                        aaGCCGACCGCAGGAATGGCGGCGAGGATCATATCCTGCGCCAACGCTAACAGAAATTCGATTACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGCCGACGCAGGTAGCCAGTGTCAGCAGACTGGCGATCGCCCAGCGTGCCAGTCCGGTATTGATGTGGCCTTTAAACATATCGGCGACGGCATTAATCAACGGAAAGCCCGGCACTAGCAGCAGAACGCTGGCGGCCATCGCAAtg                                                         <  1:222867/251‑1 (MQ=255)                                                                                                                                                                                             gACCGCAGGAATGGCGGCGAGGATCATATCCTGCGCCAACGCTAACAGAAATTCGAGTACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGCCGACGCAGGTAGCCAGTGTCAGCAGACTGGCGATCGCCCAGCGTGCCAGTCCGGTATTGATGTGGCCTTTAAACATATCGGCGACGGCATTAATCAACGGAAAGCCCGGCACTAGCAGCAGAACGCTGGCGGCCATCGCAATGGTggg                                                    <  2:228139/251‑1 (MQ=255)                                                                                                                                                                                               ccGCAGGAATGGCGGCGAGGATCATAACCTGCGCCAACGCTAACAGAAATTCGATTACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGCCGACGCAGGTAGCCAGTGTCAGCAGACTGGCGATCGCCCAGCGTGCCAGTCCGGTATTGATGTGGCCTTTAAACATATCGGCGACGGCATTAATCAACGGAAAGCCCGGCACTAGCAGCAGAACGCTGGCGGCCATCGCAATGGTggggg                                                  <  1:233076/251‑1 (MQ=255)                                                                                                                                                                                                         ggcggcGAGGATCATATCCTGCGCCAACGCTAACAGAAATTCGATTACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGCCGACGCAGGTAGCCAGTGTCAGCAGACTGGCGATCGCCCAGCGTGCCAGTCCGGTATTGATGTGGCCTTTAAACATATCGGCGACGGCATTAATCAACGGAAAGCCCGGCACTAGCAGCAGAACGCTGGCGGCCATCGCAATGGTGGGGGTATTGCTgg                                         >  2:75446/1‑249 (MQ=255)                                                                                                                                                                                                             gcgatgaccATATCCTGCGTCGACGCTAACAGAAATGCGATTCCTCCCCTCCGCGTTGCTCCCAAATCGTCTGTGCCAGCACTACGCCGACGCAGGTAGCCAGTGTCAGCAGACTGTTGCTCGCCCAGCGTTACAGTTCGGTATGGATGTGGGCTTTAAACTTATCGGCAACGGCATTAATCAGCGGAAAGCCCGGCATTAGCATCAGAACGCTGGCGGCCATCGCAATGGTGGGCGTATTGCTGAAAGTg                                    <  1:114691/243‑1 (MQ=255)                                                                                                                                                                                                                      atatCCTGCGCCAACGCTAACAGAAATTCGATTACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGCCGACGCAGGTAGCCAGTGTCAGCAGACTGGCGATCGCCCAGCGTGCCAGTCCGGTATTGATGTGGCCTTTAAACATATCGGCGACGGCATTAATCAACGGAAAGCCCGGCACTAGCAGCAGAACGCTGGCGGCCATCGCAATGGTGGGGGGATTGCTGAAAGTGGGGAGtggc                           >  2:166343/1‑248 (MQ=255)                                                                                                                                                                                                                            tgctacgaagCTAACAGAAATTCGCTTACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGCCGACGCAGGTAGCCGGTGTCAGCAGACTTGCGATAGCCCAGCGTGACAGTCCGGTATTGATGTGGACTTTAAACATATCGGCGACGGCATTAATCAACGGAACGCCCGGCACTAGCAGCAGAACGCTGGCGGCCATCGCAGTGGTGGGGGTATTGCTGAAAGAGGGGAGTTGCAAAAGc                     >  2:48341/10‑251 (MQ=255)                                                                                                                                                                                                                                     gCTAACAGAAATTCGATTACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGCCGACGCAGGTAGCCAGTGTCAGCAGACTGGCGATCGCCCAGCGTGCCAGTCCGGTATTGATGTGGCCTTTAAACATATCGGCGACGGCATTAATCAACGGAAAGCCCGGCACTAGCAGCAGAACGCTGGCGGCCATCGCAATGGTGGGGGTATTGCTGAAAGTGGGGAGTTGCAAAAGCAATCCGGaa            <  1:27697/251‑1 (MQ=255)                                                                                                                                                                                                                                           aGAAATTCGATTACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGCCGCCGCAGGTAGCCAGTGTCAGCAGACTGGCGATCGCCCAGCGTGCCAGTCCGGTATTGATGTGGCCTTTAAACATATCGGCGACGGCATTAATCAACGGAAAGCCCGGCACTAGCAGCAGAACGCTGGCGGCCATCGCAATGGTGGGGGTATTGCTGAAAGTGGGGAGTTGCAAAAGCAATCCGGAAAtggtg      <  1:259199/251‑1 (MQ=255)                                                                                                                                                                                                                                             aaaTTCGATTACACCCAGCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGCCGACGCAGGTAGCCAGTGTCAGCAGACTGGCGATCGCCCAGCGTGCCAGTCCGGTATTGATGTGGCCTTTAAACATATCGGCGACGGCATTAATCAACGGAAAGCCCGGCACTAGCAGCAGAACGCTGGCGGCCAGCGCAATGGTGGGGGTATTGCTGAAATTGGGGAGTTGCAAAAGCAATCCGTAAAtggtggt    <  2:119754/251‑1 (MQ=255)                                                                                                                                                                                                                                               aTTCGATTACCCCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCTCTACGCCGACGCAGGTAGCCAGTGTCAGCAGACTGGCTATCGCCCAGCGTGCCAGTCCGGTATTGATGTGGCCTTTAAACATAGCGGCGCCGGCATTAATCAACGGAAAGCCCGGCACTAGCAGCAGAACGCTGGCGGCCATCGCAATGGTGGGGGTATTGCTGAAAGTGGGGAGTTGCAAAAGCAATCCGGAAAtggtggtgg  <  2:11448/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    GGATGCGCCAGATACCAGCGCGACCATTGAATACCAATGGTACCGACCAGCATAGAAGCCATAAAGGTTGACCACTCAATATTCAACCCGCTGGTCATCAAGATCATTCGGGAACCATGACCTATCGAGCCAAGCAGCGCACACCAGCGTAACGCCCGCACGGGAACGTTGAACACCATCGCAAAGCCGACCGCAGGAATGGCGGCGAGGATCATATCCTGCGCCAACGCTAACAGAAATTCGATCACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGCCGACGCAGGTAGCCAGTGTCAGCAGACTGGCGATCGCCCAGCGTGCCAGTCCGGTATTGATGTGGCCTTTAAACATATCGGCGACGGCATTAATCAACGGAAAGCCCGGCACTAGCAGCAGAACGCTGGCGGCCATCGCAATGGTGGGGGTATTGCTGAAAGTGGGGAGTTGCAAAAGCAATCCGGAAATGGTGGTGG  >  NC_000913/4601844‑4602332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑