Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	5,603	T→G	intergenic (+73/+80)	yaaX → / ← yaaA	DUF2502 domain‑containing protein YaaX/DNA binding and peroxide stress response protein YaaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	5,603	0	T	G	100.0%	69.7 / NA	27	intergenic (+73/+80)	yaaX/yaaA	DUF2502 domain‑containing protein YaaX/DNA binding and peroxide stress response protein YaaA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (13/14);  total (13/14)

ATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCA‑TGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAATCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCG  >  NC_000913/5364‑5839                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                             aTTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAAGGGCGAGGCAATCGCTGGCCCCCACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCA‑TGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTGAATTCTGCa                                                                                                                                                                                                                                     <  1:227113/249‑1 (MQ=255)   accggggagggaGTTCACGGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCCCCCACCCGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCA‑TGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTGAATTCTGCAata                                                                                                                                                                                                                                  <  2:95566/246‑1 (MQ=255)    aCTGGGATGGAGGTCACGGGCGCGTCCACGGCTGGGGGAAACAACATTATGAAGGGCGAGGCAATCGCTGGCACCCACACGGACCCCCGCCCCCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCA‑TGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTGAATTCTGCAatat                                                                                                                                                                                                                                 <  2:186907/250‑1 (MQ=255)                           gACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCAGGCACCCACACGGACCGCCGACACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCAACGAACCGGGCAA‑CCTCCACGCTAAATAACAAATGCCGGGTAACAAACCGGCCATCACAGCCTGATGCGCCGCTGGCGCGCCTTAAAAAGCCTAAGTGAGTTCTGCAATATCTTGCATCCGCATGCTTTTGTAgg                                                                                                                                                                                                         >  1:240717/1‑251 (MQ=255)                                         gAAACAACATTATGAATGGCGAGGCCATCGCTGGCACCCACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCC‑TGGTCCAAGAAAACATCCCCCCTAAATGACAAATGCCGGGTAACAATCCGGCATTTACCGCCTGGTGCGACGCTGGCGCGTCTTATCAAGCCTACGTGACTTCTGCAATATATTGAATCTGCATGCTTTTTTAGGCCGGATAAGGGGtt                                                                                                                                                                                           >  2:161415/1‑251 (MQ=255)                                                            cGAGGCAATCGCTGGCACCCACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCA‑TGGGCCAGGCAAACATCACCGCTAAATGACAAATTCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTGAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTg                                                                                                                                                                        >  1:134359/1‑251 (MQ=255)                                                             gAGGCAATCGCTGGCACCCACCCGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCA‑TGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTGAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTGa                                                                                                                                                                       <  1:147697/251‑1 (MQ=255)                                                                gCAATCGCTGGCACCCACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCA‑TGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTGAATTCTGCAATATAtt                                                                                                                                                                                                                              >  1:48208/1‑193 (MQ=255)                                                                        tGGCACCCACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGGCA‑TGGTCCCGGCAAAAATTACCGCTAAATGACAAATGACGGGTAAAAAACAGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCCTATCAGGCCTACGTGGATTCTGAAATATATTGAATATGCATGCTTTTGTAGGGCGGGTAAGGCGTTCAAGCCGCATCCGGCATTGACTGCCAACtt                                                                                                                                                             >  1:259537/1‑250 (MQ=255)                                                                           cccccacacGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCA‑TGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTGAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACg                                                                                                                                                         <  1:256991/246‑1 (MQ=255)                                                                                 cccGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCA‑TGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTGAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTc                                                                                                                                                   <  1:81501/249‑1 (MQ=255)                                                                                    ggcccgccgCCACCGCCGCGCCACCATAAGAAAGCTCCACATGAACATCACGGCGGTCA‑TGGTCCAGGCAAACTTCACCGCTAAATGACAAATGCCGGGAAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTGAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGTAAACTTAACGCTGCTCGTa                                                                                                                                                <  2:76910/248‑1 (MQ=38)                                                                                     gACCGCCGCAACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGGCA‑TGGTCCAGGCCAACATCACCGCCAAAAGACCAATGCCGGGGAACAATCCGGCATTAAACGCCTGATGCGACGCTGGCGCGTCTTATCAGGTCTAAGTGGATTCTGCAATATATTGAATCTGCATTCTTTTGTAGGCCGGATAAGGCGGTCACGCCGCCTCCGGCCTTGACTGCAAACTTAACGCTGATCGt                                                                                                                                                 >  1:80655/1‑249 (MQ=255)                                                                                              cACCGCCGCGCCACCATAAGCAAGCTCCTCATGATCATCACGGCGGTCA‑TGGTCCAGGCAAACCTCACCGCTAAATGACAAATGCCGGGGAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAAGCCTACGTGAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGGCGGGTAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAac                                                                                                                                      >  1:59419/1‑251 (MQ=255)                                                                                                           ccaTAAGAAAGCTCCTCATGATCATCACGGCGGTCA‑TGGTCCAGGCAAACCTCACCGCTAAATGAACAATGCCCGGCAACAATCCGGCATTCAGCGCCCGATGCGACGCTGGCGCGTCTTATCAAGCCTAAGTGAATTCTGCAATATATTGAATCTGCATGCTTTTTTAGGCCGGATAAGGCGTTCCCGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCAt                                                                                                                         >  1:128001/1‑251 (MQ=255)                                                                                                                  aacgCTCATCATGATCAGCACGGCGGTCA‑TGGTCCAGGCAAACAGCACCGCTAAATGACAAATGACGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTGAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCCGGATAAGGCGTTCCCGCCGCATACGGCATTGACTGCAAAATTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTgga                                                                                                                  <  2:3536/248‑1 (MQ=255)                                                                                                                                    caCGGCGGTCA‑TGGTGCAGGGCACCATCACCGCTAAATGACAAATGCCCGGTAACAATCCGGCATTTAGCGCCCGCTGCGACGCTGGCGCGTCTTATCAGGCCTACGAGAATTCTGCCATATATTGAATCTGCCTGCTTTTGTCGGCCGGGTAAAGCGTTCACGCCGCATCCCGCATTGACTGCAAACTTTAAGCTGCTCGGAGCGTTTAAACACCAGTTCGCCCTTCCTGGAGGGAGCTTCCTCAaaga                                                                                                 >  2:94992/1‑250 (MQ=255)                                                                                                                                           gTCA‑TGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTGAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACgct                                                                                                                                                       >  1:7601/1‑189 (MQ=255)                                                                                                                                           gTCA‑TGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTGAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACgct                                                                                                                                                       <  2:7601/189‑1 (MQ=255)                                                                                                                                                  gTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTACCAGGCCTACGTGAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAAGCTTCATCAAAGAAGTAACCTTCGCTa                                                                                   <  2:59419/251‑1 (MQ=255)                                                                                                                                                                  ccGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTGAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAAGCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGtt                                                                   >  1:1740/1‑251 (MQ=255)                                                                                                                                                                       aaaTGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTGAATTCTGCAATATATTGTATCTGCATGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAAGCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGctc                                                               <  2:1473/250‑1 (MQ=255)                                                                                                                                                                        aaTGACAAATGCCGGGTAACAATCCGGCAGTCAGCGCTGGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTGAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAATCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTg                                                             <  1:6087/251‑1 (MQ=255)                                                                                                                                                                                                 ggCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTGAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAAGCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCaataat                                    >  2:90933/1‑251 (MQ=255)                                                                                                                                                                                                       cAGCGCCTGATGCGACGCGGGCGCGTCTTATCAGGCCTACGTGAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAAGCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATAAAAc                               <  1:126377/250‑1 (MQ=255)                                                                                                                                                                                                                               gTCTTATCAGGCCTACGTGAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAAGCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATAAAACGACTCATCAGACCGCGTGCTTTCtt      >  2:142485/1‑251 (MQ=255)                                                                                                                                                                                                                                    aTCAGGCCTACGTGAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAAGCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATAAAACGACTCATCAGACCGCGTGCTTTCTTAGCg  <  1:46379/250‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                             ATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCA‑TGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTTAACGCTGCTCGTAGCGTTTAAACACCAGTTCGCCATTGCTGGAGGAATCTTCATCAAAGAAGTAACCTTCGCTATTAAAACCAGTCAGTTGCTCTGGTTTGGTCAGCCGATTTTCAATAATGAAACGACTCATCAGACCGCGTGCTTTCTTAGCG  >  NC_000913/5364‑5839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑