| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,591,930 | G→C | E92Q (GAG→CAG) | tsr → | methyl‑accepting chemotaxis protein Tsr |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,591,930 | 0 | G | C | 95.2% | 56.6 / ‑6.2 | 21 | E92Q (GAG→CAG) | tsr | methyl‑accepting chemotaxis protein Tsr |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base C (10/10); minor base A (0/1); total (10/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCTGGTTTTGGCCGTTTTTGGCCTTTTACAACTGACATCAGGCGGTCTGTTCTTTAATGCCTTAAAGAATGACAAAGAAAATTTCACTGTTTTACAAACCATTCGCCAGCAGCAATCCACGCTGAATGGCAGCTGGGTCGCGTTGTTGCAGACGCGTAACACCCTCAACCGCGCGGGTATCCGCTACATGATGGATCAGAATAATATTGGTAGCGGTTCAACCGTTGCTGAGCTGATGGAGAGTGCCAGTATTTCGCTGAAACAGGCGGAAAAAAACTGGGCGGATTACGAAGCGTTGCCGCGTGACCCGCGTCAGAGCACCGCCGCAGCGGCAGAGATCAAACGTAATTACGATATTTATCACAATGCGCTGGCGGAGCTGATCCAACTGTTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATG > NC_000913/4591691‑4592158 | ggctgGTTGTGGCCTTTTTTGGTCTTTTACAACCGACATCAGGCGGTCTGTTCTTTAATGCCTTAAAGAATGACAAAGAAAATTTCTCTGTTTTACAATCCAATCGCCAGCAGCGATCCACTCTGAATGGCAGCTGGGGCGCGTTGTTGCAGTGGCGTAACACCCTCAACCGGGCGGGTTTCCGCTACATGAGAAATAATAATAATATTGGTAGCGGGTCAACCGTTGCTGAGCTGATGCAGAGtacaagt < 2:248928/250‑7 (MQ=255) gctgGTTTTGGCCGTTTTTGGCCTTTTACAACTGACATCAGGCGGTCTGTTCTTTAATGCCTTAAAGAATGACAAAGAAAATTTCACTGTTTTACAAACCATTCGCCAGCAGCAATCCACGCTGAATGGCAGCTGGGTCGCGTTGTTGCAGACGCGTAACACCCTCAACCGCGCGGGTATCCGCTACATGATGGATCAGAAGAATATTGGTAGCGGTTCAACCGTTGCTGAGCTGATGCAGAGTGCCAGt < 2:190242/250‑1 (MQ=255) ggCCGTTTTTGGCCTTTTACAACTGACATCAGGCGGTCTGTTCTTTAATGCCTTAAAGAATGACAAAGAAAATTTCACTGTTTTACAAACCATTCGCCAGCAGCAATCCACGCTGAATGGCAGCTGGGTCGCGTTGTTGCAGACGCGTAACACCCTCAACCGCGCGGGTATCCGCTACATGATGGATCAGAATAATAGTGGTAGCGGTTCAACCGTTGCTGAGCTGAGGCAGAGTGCCAGTATTTCGCTg < 2:26649/250‑1 (MQ=255) aaCTGACAGCAGGCGGTCTGTTCTTTAATGCCTTAAAGAATGACAAATAAAATTTCACTGTTTTACAAACCATGCGCCAGCAGCAATCCACGCTGAATGGCAGCTGGGTCGCGTTGTTGCAGACGCGTAACACCCTCAACCGCGCGGGTATCCGCTACATGATGTATCATAATAATATGTGTATCGGTTCAACCGTTGCTGAGCTGATGCAGAGTGCCAGTATTTCGCTGAAACAGGCGGAAAAAAACTgg < 1:161866/251‑1 (MQ=255) aCATCAGGCGGTCTGTTCTTTAATGCCTTAAAGAATGACAAAGAAAATTTCACTGTTTTACAAACCATTCGCCAGCAGCAATCCACGCTGAATGGCAGCTGGGTCGCGTTGTTGCAGACGCGTAACACCCTCAACCGCGCGGGTATCCGCTACATGATGGATCAGAATAATATTGGTAGCGGTTCAACCGTTGCTGAGCTGATGCAGAGTGCCAGTATTTCGCTGAAACAGGCGGAAAAAAACTGGGc > 2:9848/1‑248 (MQ=255) ggCGGTCTGTGCTTTAATGCCGTAAAGAATGACAAAGAAAATGTCACTGTTTTACAAACCAGTCGCCAGCAGCAATCCACGCTGAATGGCAGCTGGGTCGCGTTGATGCAGACGCGTAACACCCTCAACCGCGCGGGTATCCGCTACATGATGGATCAGAATAATATTGGTAGCGGTTCAACCGTTGCGGAGCTGATGCAGAGTGCCAGTATGTCGCTGAAACAGGCGGAAAAAAACTGGGCAGATTACGa < 2:72650/251‑1 (MQ=255) aaTGCCTTAAAGAATGACAAAGAAAATTTCACTGTTTTACAAACCATTCGCCAGCAGCAATCCACGCTGAATGGCAGCTGGGTCGCGTTGTTGCAGACGCGTAACACCCTCAACCGCGCGGGTATCCGCTACATGATGGATCAGAATAATATTGGTAGCGGTTCAACCGTTGCTGAGCTGATGCAGAGTGCCAGTATTTCGCTGAAACAGGCGGAAAAAAACTGGGCAGATTACGAAGCGTTGCCGCGTg > 1:121145/1‑250 (MQ=255) gttgttccaacccATTCGCCAGCAGCAATCCCCGCTGAATGGCAGCTGGGTCGCGTTGTTGCAGACGCGTAACACCCTCAACCGCGCGGGTATCCGCTACATGATGGATCAGAATAATATGGGTAGCGGTTCAACCGTTGCTGAGCTGATGCAGAGTGCCAGTATTTCGCTGAAACAGGCGGAAAAAAACTGGGCAGATGACGAAGCGTTGCCTCGTGACCCGCGTCAGAGCACCGCCGCAGCGGCagag < 2:70799/239‑1 (MQ=255) cGCCAGCAGCAATCCACGCTGAATGGCAGCTGGGTCGCGTTGTTGCAGACGCGTAACACCCTCAACCGCGCGGGTATCCGCTACATGATGGATCAGAATAATATTGGTAGCGGTTCAACCGTTGCTGAGCTGATGCAGAGTGCCAGTATTTCGCTGAAACAGGCGGAAAAAAACTGGTCAGATTACGAAGCGTTGCCGCGTGACCCGCGTCAGAGCACCGCCGCAGCGGCAGAGATCAAACGTAATTACg > 1:174832/1‑250 (MQ=255) gCCAGCAGCAATCCACGCTGAATGGCAGCTGGGTCGCGTTGTTGCAGACGCGTAACACCCTCAACCGCGCGGGTATCCGCTACATGATGGATCAGAATAATATTGGTAGCGGTTCAACCGTTGCTGAGCTGATGCAGAGTGCCAGTATTTCGCTGAAACAGGCGGAAAAAAACTGGGCAGATTACGAAGCGTTGCCGCGTGACCCGCGTCAGAGCACCGCCGCAGCGGCAGAGATCAAACGTAATTACGa > 2:60450/1‑250 (MQ=255) tCCACGCTGAATGGCAGCTGGGTCGCGTTGTTGCAGACGCGTAACACCCTCAACCGCGCGGGTATCCGCTACATGATGGATCAGAATAATATTGGTAGCGGTTCAACCGTTGCTGAGCTGATGCAGAGTGCCAGTATTTCGCTGAAACAGGCGGAAAAAAACTGGGCAGATTACGAAGCGTTGCCGCGTGGCCCGCGTCAGAGAACCGCCGCAGCGGCAGAGATAAAACGCAATACCGATATTTATcaa > 2:231897/1‑248 (MQ=255) gAATGGCAGCGGGGTCGCGTTGTTGCAGACGCGTAACACCCTCAACCGCGCGGGTATCCGCTACATGATGGATCAGAATAATATTGGTAGCGGTTCAACCGTTGCTGAGCTGATGCAGAGTGCCAGTATTTCGCTGAAACAGGCGGAAAAAAACTGGGCAGATTACGAAGCGTTGCCGCGTGACCCGCGTCAGAGCACCGCCGCAGCGTCAGAGATCAAACGTAATTACGATATTTATCACAATGCGCTg < 1:79590/250‑1 (MQ=255) tGGGTCGCGTTGTTGCAGACGCGTAACACCCTCAACCGCGCGGGTATCCGCTACATGATGGATCAGCATAATATTGGTAGCGGTTCAACCGTTGCTGAGCTGATGCAGAGTGCCAGTATTTCGCTGAAACAGGCGGCAAAAAACTGGGCAGATTACGAAGCGTTGCCGCGTGACCCGCGTCAGAGCACCGCCGCAGCGGCAGAGATCAAACGTAAtt > 1:146777/1‑217 (MQ=255) tGGGTCGCGTTGTGGCAGACGCGTAACCCCCTCAACCGCGCGGGTATCCGCTACATGATGGATAAGAATAATATTGGTAGCGGTTCAACCGTGGCTGAGCTGATGCAGAGTGCCAGTATGTCGCTGAAACAGGCGGAAAAAAACGGGGCAGATTACGAGGCGTTGCCGCGTGACCCGCGTCAGAGCACCGCCGCAGCGGCAGAGATCAAACGTAAtt < 2:146777/217‑1 (MQ=255) gttgCAGACGTGTAACACCCTAAACCGCGCGGGTATCCGCTACCTGATGGCTCAGAATAATATTGGCAGCGGTTCCACCGTTGCTGAGCTGCTGCAGCGTGCCAGTATTTCGCTGAAACAGGCGGAAAAAAACTGGGCAGATTACGAAGCGTTGCCGCGTGAccc > 2:41312/1‑165 (MQ=255) gttgCAGACGCGTAACACCCTCAACCGCGCGGGTATCCGCTACATGATGGATCAGAATAATATTGGTAGCGGTTCAACCGTTGCTGAGCGGATGCAGAGTGCCAGTATTTCGCTGAAACAGGCGGAAAAAAACTGGGCAGATTACGAAGCGTTGCCGCGTGAccc < 1:41312/165‑1 (MQ=255) tCCGCTACATGATGGATCAGAATAATATTGGTAGCGGTTCAACCGTTGCTGAGCTGATGCAGAGTGCCAGTATTTCGCTGAAACAGGCGGAAAAAAACTGGGCAGATTACGAAGCGTTGCCGCGTGACCCGCGTCAGAGCACCGCCGCAGCGGCAGAGATCAAACGTAATTACGATATTTATCACAATGCGCTGGCGGAGCTGATCCAACTATTAGGTGCCGGCAAAATCAACGAGTTCTTTGATCAGCCg > 2:75145/1‑251 (MQ=255) cGCTACATGATGGATCAGAATAATATTGGTAGCGGTGCAACCGTTGCTGAGCTGATGCAGAGTGCCAGTATTTCGCTGAAACAGGCGGAAAAAAACTGGGCAGATTACGAAGCGTTGCCGCGTGACCCGCGTCAGAGCACCGCCGCAGCGGCAGAGATCAAACGTAATTACGATATTTATCACAATGCGCTGGCGGAGCTGATCCAACTATTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGAc < 1:9848/251‑1 (MQ=255) aGAATAATATTGGGAGCGGTCCAACCGTTGATGGGCAGAGTAAGAGTGCCAGTATTTCGCGGAAACAGGCGGAAAAAAACGGGGCAGATTACGAAGCGTGGCCGCGTGACCCGGGTCAGAGCACCGCCGCAGCGGCAGAGATCAAACGTAATTACGATATTTATCACAATGCGCTGGCGGATCTGATCCAACTATTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGAACCAGGGATAGCAGGAc < 2:174832/251‑1 (MQ=255) tatTGGTAGCGGTTCAACCGTTGCTGAGCTGATGCAGCGTGCCAGTATTTCGCTGAAACAGGCGGACAAAAACTGGGCAGATTACGAAGCGTTGCCGCGTGACCCGCGTCAGAGCACCGCCGCAGCGGCAGAGATCAAACGTAATTACGATATTTATCACAATGCGCTGGCGGAGCTGATCCAACTATTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGACCCAGGGATATCAGGACGGTTTCg > 1:236951/1‑251 (MQ=255) ttCAACCGTTGCTGAGCTGATGCAGAGTGCCAGTATTTCGCTGAACCAGGCGGAAAAAAACTGGGCAGATTACGACGCGTTGCCGCGTGACCCGCGTCAGAGCACCGCCGCAGCGGCAGAGATCAAACGTAATTACGATATTTATCACAATGCGCTGGCGGAGCTGATCAAACTATTAGGTGCAGACAAAATCAACGAGTTTTTTGATCAGCCGACGCAGGGATATCAGGACGGTTTCGCGAAGCAGtctg > 1:130615/1‑248 (MQ=255) | TGCTGGTTTTGGCCGTTTTTGGCCTTTTACAACTGACATCAGGCGGTCTGTTCTTTAATGCCTTAAAGAATGACAAAGAAAATTTCACTGTTTTACAAACCATTCGCCAGCAGCAATCCACGCTGAATGGCAGCTGGGTCGCGTTGTTGCAGACGCGTAACACCCTCAACCGCGCGGGTATCCGCTACATGATGGATCAGAATAATATTGGTAGCGGTTCAACCGTTGCTGAGCTGATGGAGAGTGCCAGTATTTCGCTGAAACAGGCGGAAAAAAACTGGGCGGATTACGAAGCGTTGCCGCGTGACCCGCGTCAGAGCACCGCCGCAGCGGCAGAGATCAAACGTAATTACGATATTTATCACAATGCGCTGGCGGAGCTGATCCAACTGTTAGGTGCAGGCAAAATCAACGAGTTCTTTGATCAGCCGACCCAGGGATATCAGGACGGTTTCGAGAAGCAGTATG > NC_000913/4591691‑4592158 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |