Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,591,674	A→G	K6K (AAA→AAG)	tsr →	methyl‑accepting chemotaxis protein Tsr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,591,674	0	A	G	100.0%	32.5 / NA	12	K6K (AAA→AAG)	tsr	methyl‑accepting chemotaxis protein Tsr
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/7);  total (5/7)

CTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTACTGCTGGTTTTGGCCGTTTTTGGCCTTTTACAACTGACATCAGGCGGTCTGTTCTTTAATGCCTTAAAGAATGACAAAGAAAATTTCACTGTTTTACAAACCATTCGCCAGCAGCAATCCACGCTGAATGGCAGCTGGGTCGCGTTGTTGCAGACGCGTAACACCCTCAACCGCGCGGGTATCCGCTACATGATGGATCAGAATAATATTGGTAGCGGTTCA  >  NC_000913/4591573‑4591911                                                                                                      |                                                                                                                                                                                                                                              ccatggctggcgggttaccccggttCATAAAGTTTTTGCTTTCCAGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAGATCGTGACCAGCGTACTGCTGGTGTTGGCCGTTTTTGGCCTTTTACAACTGACACCAGGCGGTCTGTTCTTTAATGCCTTAAAGAAGGACAAAGAAAATTTCACTGTTTTACAAACCATTCGCCCGCAGCAACCCACTCTCAATGGCa                                                                                           <  2:131808/227‑1 (MQ=255)        tggcgggttacaccttttCATAACGTTTTTGCTTTCCAGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAGATCGTGACCAGCTTACTGCTGGTTTTGGCCGTTTTTGGCCTTTTACAACTGACATCAGGCGGTCTGTTCTTTAATGCCTTACAGAATGACAAAGAAAATTTCACTGTTTTACAAACCATTCGCCAGCAGCAATCCACGCTGAATGGCAGCTGGGTc                                                                                   >  2:172560/17‑251 (MQ=255)                       tttCATAAAGTTTTTGCTTTCCAGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAGATCGTGACCAGCTTACTGCTGGTTTTGGCCGTTTTTGGCCTTTTACAACTGACATCAGGCGGTCTGTTCTTTAATGCCTTAAAGAATGACAAAGAAAATTTCACTGTTTTACAAACCATTCGCCAGCAGCAATCCACGCTGAATGGCAGCTGGGTCGCGTTGTTGCAGAc                                                                     <  1:172560/249‑1 (MQ=255)                        ttCATAAAGTATTTGATTTCCAAGACGAAAATCTTGCATCGGTCCACAGGAAAGCGAAACCATGTTCACACGTATCAAGATCGTGACCAGCTTACTGCTGGTTTTGGCCGTTTTTGGCATTTTACAACTGACATCAGGCGGTCTGTTCTTTAATGCCTTAAAGAATGACAAAGAAAATTTCACTGTTTTACAAACCATTCGCCAGCAGCAATCAACGCTGAATGGCAGCTGGGTCGCGTGGTTGCAGAcgc                                                                   >  2:123198/1‑251 (MQ=255)                             aaaGTTTTTGCTTTCCAGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAGATCGTGACCAGCTTACTGCTGGTTTTGGCCGTTTTTGGCCTTTTACAACTGACATCAGGCGGTCTGTTCTTTAATGCCTTAAAGAATGACAAAGAAAATTTCACTGTTTTACAAACCATTCGCCAGCAGCAATCCACGCTGAATGGCAGCTGGGTCTCGTTGTTGCAGACGTGTAac                                                              <  2:131354/251‑1 (MQ=255)                                      gctttacAGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAGATCGTGACCAGCTTACTGCTGGTTTTGGCCGTTTTTGGCCTTTTACAACTGACATCAGGCGGTCTGTTCTTTAATGCCTTAAAGAATGACAAAGAAAATTTCACTGTTTTACAAACCATTCGCCAGCAGCAATCCACGCTGAATGGCAGCTGGGTCGCGTTGTTGCAGACGCGTAACACCCTCAAc                                                     >  2:184935/2‑251 (MQ=255)                                                          tGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAGATCGTGACCAGCTTACTGCTGGTTTTGGCCGTTTTTGGCCTTTTACAACTGACATCAGGCGGTCTGTTCTTTAATGCCTTAAAGAATGACAAAGAAAATTTCACTGTTTTACAAACCATTCGCCAGCAGCAATCCACGCTGAATGGCAGCTGGGTCGCGTTGTTGCAGACGCGTAACACCCTCAACCGCGCGGGTATCCGCTAc                                   <  1:184935/249‑1 (MQ=255)                                                           gCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAGATCGTGACCAGCTTACTGCTGGTTTTGGCCGTTTTTGGCCTTTTACAACTGACATCAGGCGGTCTGTTCTTTAATGCCTTAAAGAATGACAAAGAAAATTTCACTGTTTTACAAACCATTCGCCAGCAGCAATCCACGCTGAATGGCAGCTGGGTCGCGTTGTTGCAGACGCGTAACACCCTCAACCGCGCGGGTATCCGCTACat                                 <  2:22756/250‑1 (MQ=255)                                                             atcagacaacaggctagagAAACCACGTTAAAAAGTATAAAGATCGTGACCAGCTTACTGCTGGTTTTGGCCGCTTTTGGACTTTTACAACTGACATCAGGCGGTCTGTTCTTTAATGCCTTAAAGAATGACAACGAAAATTTCACTGTTTTACCAACCATTCGCCAGCAGCAATCCACGCTGAATGGCAGCTGGGTAGCGTTGTTGCAAACGCGTAACACCCTCAACCGCGCGGGAATCCGCTACatg                                >  2:126957/16‑249 (MQ=255)                                                                                 aaCCATGTTAAAACGTATCAAGATCGTGACCAGCTTACTGCTGGTTTTGGCCGTTTTTGGCTTTTTACAACTGACATCAGGCGGTCTGTTCTTTAATGCCTTAAAGAATGACAAAGAAAATTTCACTGTTTTACAAACCATTCGCCAGCAGCAATCCACGCTGAATGGCAGCTGGGTCGCGTTGTTGCAGACGCGTAACACCCTCAACCGCGCGGGTATCCGCTACATGATGGATCAGAATAATATTGGTa          <  2:191319/251‑1 (MQ=255)                                                                                     aTGTTAAAACGTATCAAGATCGTGACCAGCTTACTGCTGGTTTTGGCCGTTTTTGGCCTTTTACAACTGACATCAGGCGGTCTGTTCTTTAATGCCTTAAAGAATGACAAAGAAAATTTCACTGTTTTACAAACCATTCGCCAGCAGCAATCCACGCTGAATGGCAGCTGGGTCGCGTTGTTGCAGACGCGTAACACCCTCAACCGCGCGGGTATCCGCTACATGATGGATCAGAATAATATTGGTAGCgg      <  1:74722/251‑1 (MQ=255)                                                                                          aaaaCGTATCAAGATCGTGACCAGCTTACTGCTGGTTTTGGCCGTTTTTGGCATTTTACAACTGACATCAGGCGGTCTGTTCTTTAATGCCTTAAAGAATGACAAAGAAAATTTCACTGTTTTACAAACCATTCGCCAGCAGCAATCAACGCTGAATGGCAGCTGGGTCGCGTTGTTGCAGACGCGAAACACCCTCAACCGCGCGGGTATCCGCTACATGATGGATCAGAATAATATTGGGAGCGGTTCa  >  1:150703/1‑250 (MQ=255)                                                                                                      |                                                                                                                                                                                                                                              CTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTACTGCTGGTTTTGGCCGTTTTTGGCCTTTTACAACTGACATCAGGCGGTCTGTTCTTTAATGCCTTAAAGAATGACAAAGAAAATTTCACTGTTTTACAAACCATTCGCCAGCAGCAATCCACGCTGAATGGCAGCTGGGTCGCGTTGTTGCAGACGCGTAACACCCTCAACCGCGCGGGTATCCGCTACATGATGGATCAGAATAATATTGGTAGCGGTTCA  >  NC_000913/4591573‑4591911

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑