| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,567,272 | C→A | intergenic (‑26/+15) | yjiN ← / ← mdtM | DUF445 domain‑containing protein YjiN/multidrug efflux pump/bile salt:H(+) antiporter/Na(+):H(+) antiporter/K(+):H(+) antiporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,567,272 | 0 | C | A | 94.7% | 46.9 / ‑4.2 | 21 | intergenic (‑26/+15) | yjiN/mdtM | DUF445 domain‑containing protein YjiN/multidrug efflux pump/bile salt:H(+) antiporter/Na(+):H(+) antiporter/K(+):H(+) antiporter |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base A (10/8); total (11/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.31e-01 | |||||||||||
CGTATGGCGAGAAATGATCGGAATCGGCACGCGGCGAAACAGCGCCACCACCGCAAACCAGTCCGCCAGCGCGCCGACCATCGCCGCTTCAGCAATCGCCTTCACGCCGCTCACCCAAAAATTGGGCGGC‑AAAAACA‑GCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAA‑GAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCCGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCACGCGATTGAGCAATCCCGCCAGGGTGAAAACGACGATAACGCCCGCCACAACGGCTAACAGATGAAACGGCAAGCGACCGCCGTTAAACCATAGCCAGCGGCCGATTTCGACCGAGACCGACATCACCATCAGGATCACCATATT > NC_000913/4567025‑4567472 | cGTATGGCGGGAAATGATCGGAACCGGCACGCGGCGAAACAGCGCCACCACCGCAAACCAGTCCGCCAGCGCGCCGACCATCGCCGCTTCAGCAATCGCCTGCACGCCGCTCACCCAAAAATTGGGCGGC‑AAAAACA‑GCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAA‑GAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGa < 2:169605/251‑1 (MQ=255) gTATGGCGAGAAATGATCGGAATCGGCACGCGGCGAAACAGCGCCACCACCGCAAACCAGTCCGCCAGCGCGCCGACCATCGCCGCTTCAGCAATCGCCTTCACGCCGCTCACCCAAAAATTGGGCGGC‑AAAAACA‑GCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAA‑GAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGAt < 1:219569/251‑1 (MQ=255) aTCGGAATCGGCACGCGGCGAAACAGCGCCACCACCGCAAACCAGTCCGCCAGCGCGCCGACCATCGCCGCTTCAGCAATCGCCTTCACGCCGCTCACCCAAAAATTGGGCGGC‑AAAAACA‑GCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAA‑GAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACt < 1:259118/251‑1 (MQ=255) cACGCGGCGAAACAGCGCCACCACCGCAAACCAGTCCGCCAGCGCGCCGACCATCGCCGCTTCAGCAATCGCCTTCACGCCGCTCACCCAAAAAGTGGGCGGC‑AAAAACA‑GCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAA‑GAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACt < 1:236763/251‑1 (MQ=255) aGCGCCACCACCGCAAACCAGCCCGCCAGCGCGCCGACCATCGCCGCTTCAGCAATCGCCTTCACGCCGCTCACCCAAAAATTGGGCGGC‑AAAAACA‑GCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAA‑GAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCGAAAATCACTGCTCCTCCACTGGCGCGGCTGCCt < 1:86117/251‑1 (MQ=255) cccAGTCCGCCATCGCGCCAACCACCGCCGCTTCAGCAATCGCCTTCACGCCGCTCACCCAAAAATTGGGCGGC‑AAAAACA‑GCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAA‑GAGAGCGCCATCCTTTGGGCGCGTCTGAGTTCGAGGAGTTTATTCAGAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTATCTCGGCTGCATGATGCTGGCGTAcgcg < 2:247842/250‑1 (MQ=255) cAGTCCGCCAGCGCGCCGACCATCGCCGCTTCAGCAAGCGCCTTCACGCCGCTCACCCAAAAATTGGGCGGC‑AAAAACA‑GCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAA‑GAGAGCGCCAACCTTTGGGCGCGTCTGAGTTCGATGAGGTTATTCATAACGTTAAGAATAGAGCCTGACGGCTGGATGGCGCAAAAATCTCTGCTCCTCCACTATCTCGGCTGCCTGATGCTGGCGCACGCGAt < 2:263397/251‑1 (MQ=255) gcgcCGACCATCGCCGCTTCAGCAATCGCCTTCACGCCGCTCACCCAAAAATTGGGCGGC‑AAAAACA‑GAGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAA‑GAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCACAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCACGCGATTGAGCAATCCCg > 2:140064/1‑251 (MQ=255) gcctaCCATCGCCGCTTCAGCAATCGCCTTCACGCCGCTCACCCAAAAATTGGGCGGC‑AAAAACA‑GCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAA‑GAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGACCGCGCAAAAATCACTGCTCCTCCACTAGCTCTGCTGCCTGATGCTGGCGCACGCGCTTGAGCAATCCCGcc > 2:80187/5‑251 (MQ=255) cGACCATCGCCGCTCCAGCAATCGCCTTCACGCCGCTCACCCAAAAATTGGGCGGC‑AAAAACA‑GCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAA‑GAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCACGCGATTGAGCAATCCCGCCAg < 1:191792/251‑1 (MQ=255) cGCCTTCACGCCGCTCACCCAAAAATTGGGCGGC‑AAAAACA‑GCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAA‑GAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCACGCGATTGAGCAATCCCGCCAGGGTGAAAACGACGATAAcgccc > 1:248076/1‑251 (MQ=255) ccTTCACGCCGCTCACACAAAAATTGGGCGGCTAAAAAC‑‑GCGTAACGACACAGGTATCAGCGGCGCTAAGCAGTAAA‑GATAGCTCCACCTTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTGACTCGGCTGTCTGACGCTGCCGCCCGCCATTGCGCACTCTCGCCAGGGTGATCACGACAGTAAcgccccc > 2:198904/1‑249 (MQ=255) tCACCCAAAAATTGGGCGGC‑AAAAACA‑GCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAA‑GAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCACGCGATTGAGCAATCCCGACAGGGTGAAAACGACGATAACGCCCGCCCCAACGGCTa > 1:92641/1‑250 (MQ=255) gggcgc‑AAAAACAACCGTACCGACAAAGGTAGCAGCGGC‑ATAAATAATAAAAGAGAGCGCAACCCTTTGGGCGCTTCTGATTCCGATGTGTTTATTAATAAGTTTAAAAATAGGGCCGGCCGGCGGAATCGCACAAAAATCCCTGCTCCGCCACAAGCTCGTCTGCCTAATGCTGGCGCCCGTGATTGAGCAATCCCGCGAGGGTAAAAACGACGAAACCGCCCGCCACAACGGCTAACAGATGAAACGGc < 1:252036/247‑1 (MQ=255) ggc‑AAAAACA‑GCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAA‑GAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCACGCGATTGAGCAATCCCGCCAGGGTGAAAACGACGATAACGCCCGCCACAACGGCTAACAGATGAAACGGCAAGc > 1:11575/1‑251 (MQ=255) c‑AAAAACA‑GCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAA‑GAGAGCGCCAACCATTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCACGCGATTGAGCAATCCCGCCAGGGTGAAAACGACGATAACGCCCGCCACAACGGCTAACAGATGAAACGGCAAGCg > 1:112364/1‑250 (MQ=255) aaaCA‑GCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAA‑GAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCCGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCACGCGATTGAGCGATCCCGCCAGGGTGAAAACGACGATAACGCCCGCCACAACGGCTAACAGATGAAACGGCAAGCGAccg > 2:151886/1‑251 (MQ=255) cAAAGGTAGCAGCGGCGATAAGCAGTAAA‑GAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCACGCGATTGAGCAATCCCGCCAgg < 1:209856/180‑1 (MQ=255) cAAAGGTAGCAGCGGCGATAAGCAGTAAA‑GAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCACGCGATTGAGCAATCCCGCCAgg > 2:209856/1‑180 (MQ=255) cgTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCACGCGATTGAGCAATCCCGCCAGGGTGAAAACGACGATAACGCCAGCCACAACGGCGAACAGATGAAACGGCAAGCGACCGCCGTTAAACCATAGCCAGAGGCCGATTTCGACCGAGACCGTCATCAACCTCAGCAACACCatat > 1:150651/1‑251 (MQ=255) gTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCAGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCACGCGATTGAGCAATCCCGCCAGGGTGAAAACGACGATAACGCCCGCCACAACGGCTAACAGATGAAACGGCAAGCGACCGCCGTTAAACCATAGCCAGCGGCCGATTTCGACCGAGACCGACATCACCATCAGGATCACCATAtt > 1:147570/1‑251 (MQ=255) | CGTATGGCGAGAAATGATCGGAATCGGCACGCGGCGAAACAGCGCCACCACCGCAAACCAGTCCGCCAGCGCGCCGACCATCGCCGCTTCAGCAATCGCCTTCACGCCGCTCACCCAAAAATTGGGCGGC‑AAAAACA‑GCGTAACGACAAAGGTAGCAGCGGCGATAAGCAGTAAA‑GAGAGCGCCAACCTTTTGGCGCGTCTGAGTTCGATGAGTTTATTCATAACGTTAAGAATAGAGCCTGACGGCCGGATCGCGCAAAAATCACTGCTCCTCCACTAGCTCGGCTGCCTGATGCTGGCGCACGCGATTGAGCAATCCCGCCAGGGTGAAAACGACGATAACGCCCGCCACAACGGCTAACAGATGAAACGGCAAGCGACCGCCGTTAAACCATAGCCAGCGGCCGATTTCGACCGAGACCGACATCACCATCAGGATCACCATATT > NC_000913/4567025‑4567472 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |