Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	5,442	T→C	L70P (CTA→CCA)	yaaX →	DUF2502 domain‑containing protein YaaX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	5,442	0	T	C	100.0%	41.5 / NA	19	L70P (CTA→CCA)	yaaX	DUF2502 domain‑containing protein YaaX
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/6);  total (13/6)

ATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCA‑TGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAA‑CAATCCGGCATTCAGCGCCTGATGCGACG‑CTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTT  >  NC_000913/5205‑5684                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                      aTAACGGGCAATGATAAAAGGAGTAACCTATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCCACACGGAccgccg                                                                                                                                                                                                                                          >  1:260674/1‑251 (MQ=255)      gggCAATGATAAAAGGAGTAACCTATGAAAAATATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCGGGTGGAAACAACATTATGAATGGCGAGGGAATCGCTGGCACCCACACGGACcgccgcaaac                                                                                                                                                                                                                                     >  2:46379/1‑247 (MQ=255)                                           aaTCTATCGTACTCGCACTTTCCCTGGCTCTGGACGCTCCCATGGCAGCACAGGCTGCGGAATTTACGTTAGACCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGCGGCAATCGCTGGCACCCACACGGACCGCCGCCACCGCCGCGCCCCCATAAGAAAGATCCTCATGACCATCAc                                                                                                                                                                                                >  1:11812/1‑251 (MQ=255)                                                                            ccGCTCCCATGGCGTCCAAGGCTGCGGAAATTACGTGAGTCCCGTCGGTAAAATTACAGATAGGCGATCGTGATAATCTTGGTTATTACGGGGATGGAGGTCACTGGCGGGACCCCGGCTGGTGGAAACAACATTGTAAATGGCGAGGCAATCGCTGGCACCCACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCA‑TGGTCCAGGCAAACATCACCGCTaa                                                                                                                                                              <  2:61310/250‑1 (MQ=255)                                                                                tCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCCACACGGAACGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCA‑TGGTCCAGGCaaaacaccacactaaata                                                                                                                                                           >  2:227113/1‑235 (MQ=255)                                                                                                        aaTTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCCACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCA‑TGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAA‑CAATCCGGCa                                                                                                                                 <  1:5199/251‑1 (MQ=255)                                                                                                                    cccGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCCACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCC‑TGGTCCACGCAAAACTCACCCCTAAAAGACAAACTCCGGGGAA‑CAAACCGGCATTCacctcccgc                                                                                                                     >  1:3536/1‑243 (MQ=255)                                                                                                                       gTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCCACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCA‑TGGTACAGGGAAACATCACCGCTAAATTACAAAAGCCGGGGAA‑CAATCCGGCATTCAACGCCTgctgg                                                                                                                  >  1:65094/1‑247 (MQ=255)                                                                                                                                                cGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCCACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCA‑TGGGCCCGGCAAAAATCACCGCTAAATGACAAATACCGGGAAAACAA‑CCGGCATTCCACGCCTGGTGCGACGACTG‑CGCGTCTTATCAAGccc                                                                                        >  1:211458/1‑250 (MQ=255)                                                                                                                                                                aTTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAAGGGCGAGGCAATCGCTGGCCCCCACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCA‑TGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAA‑CAATCCGGCATTCAGCGCCTGATGCGACG‑CTGGCGCGTCTTATCAGGCCTACGTGAATTCTGCa                                                                          <  1:227113/249‑1 (MQ=255)                                                                                                                                                                  accggggagggaGTTCACGGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCCCCCACCCGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCA‑TGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAA‑CAATCCGGCATTCAGCGCCTGATGCGACG‑CTGGCGCGTCTTATCAGGCCTACGTGAATTCTGCAata                                                                       <  2:95566/246‑1 (MQ=255)                                                                                                                                                                   aCTGGGATGGAGGTCACGGGCGCGTCCACGGCTGGGGGAAACAACATTATGAAGGGCGAGGCAATCGCTGGCACCCACACGGACCCCCGCCCCCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCA‑TGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAA‑CAATCCGGCATTCAGCGCCTGATGCGACG‑CTGGCGCGTCTTATCAGGCCTACGTGAATTCTGCAatat                                                                      <  2:186907/250‑1 (MQ=255)                                                                                                                                                                       ggATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCCACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGGCA‑TGGTCCAGGCCAACCTCCCCGCTAAATGACAAATGCCGGGGAA‑CACTCCGGCATTCACCGCCTGATGCCACC‑CTGGCGCGTCTTATCAGGCCTACGtggattttgccatattttgt                                                                 >  2:126377/1‑232 (MQ=255)                                                                                                                                                                                          gACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCAGGCACCCACACGGACCGCCGACACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCAACGAACCGGGCAA‑CCTCCACGCTAAATAACAAATGCCGGGTAA‑CAAACCGGCCATCACAGCCTGATGCGCCG‑CTGGCGCGCCTTAAAAAGCCTAAGTGAGTTCTGCAATATCTTGCATCCGCATGCTTTTGTAgg                                              >  1:240717/1‑251 (MQ=255)                                                                                                                                                                                                        gAAACAACATTATGAATGGCGAGGCCATCGCTGGCACCCACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCC‑TGGTCCAAGAAAACATCCCCCCTAAATGACAAATGCCGGGTAA‑CAATCCGGCATTTACCGCCTGGTGCGACG‑CTGGCGCGTCTTATCAAGCCTACGTGACTTCTGCAATATATTGAATCTGCATGCTTTTTTAGGCCGGATAAGGGGtt                                >  2:161415/1‑251 (MQ=255)                                                                                                                                                                                                                           cGAGGCAATCGCTGGCACCCACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCA‑TGGGCCAGGCAAACATCACCGCTAAATGACAAATTCCGGGTAA‑CAATCCGGCATTCAGCGCCTGATGCGACG‑CTGGCGCGTCTTATCAGGCCTACGTGAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTg             >  1:134359/1‑251 (MQ=255)                                                                                                                                                                                                                            gAGGCAATCGCTGGCACCCACCCGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCA‑TGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAA‑CAATCCGGCATTCAGCGCCTGATGCGACG‑CTGGCGCGTCTTATCAGGCCTACGTGAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTGa            <  1:147697/251‑1 (MQ=255)                                                                                                                                                                                                                               gCAATCGCTGGCACCCACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCA‑TGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAA‑CAATCCGGCATTCAGCGCCTGATGCGACG‑CTGGCGCGTCTTATCAGGCCTACGTGAATTCTGCAATATAtt                                                                   >  1:48208/1‑193 (MQ=255)                                                                                                                                                                                                                                       tGGCACCCACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGGCA‑TGGTCCCGGCAAAAATTACCGCTAAATGACAAATGACGGGTAA‑AAAACAGGCATTCAGCGCCTGATGCGACG‑CTGGCGCGTCCTATCAGGCCTACGTGGATTCTGAAATATATTGAATATGCATGCTTTTGTAGGGCGGGTAAGGCGTTCAAGCCGCATCCGGCATTGACTGCCAACtt  >  1:259537/1‑250 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                      ATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAATTACAGATAGGCGATCGTGATAATCGTGGCTATTACTGGGATGGAGGTCACTGGCGCGACCACGGCTGGTGGAAACAACATTATGAATGGCGAGGCAATCGCTGGCACCTACACGGACCGCCGCCACCGCCGCGCCACCATAAGAAAGCTCCTCATGATCATCACGGCGGTCA‑TGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAA‑CAATCCGGCATTCAGCGCCTGATGCGACG‑CTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATTGACTGCAAACTT  >  NC_000913/5205‑5684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑