Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,560,309	T→A	P124P (CCA→CCT)	yjiH ←	uncharacterized protein YjiH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,560,309	0	T	A	100.0%	72.1 / NA	24	P124P (CCA→CCT)	yjiH	uncharacterized protein YjiH
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (13/11);  total (13/11)

GCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCAAACACCGCGACGCCGGAAGAGAAGTAGTTGGTGATGATGGCGCTACCGCTGGTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCC‑ACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATC  >  NC_000913/4560064‑4560542                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                           gCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCAAACACCGCGACGCCGGAAGAGAAGTAGTTGGTGATGATGGCGCTACCGCTGGTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAAt                                                                                                                                                                                                                                       <  1:214909/251‑1 (MQ=255)  cTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCAAACACCGCGACGCCGGAAGAGAAGTAGTTGGTGATGATGGCGCTACCGCTGGTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCCTCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATg                                                                                                                                                                                                                                      >  2:212255/1‑251 (MQ=255)           aTTTAAAAACCAGGATGACGGCTAAAGGTACGATTACGGACGGGCCCTGAAAGGCAAACACCGCGACGCCGGAAGAGAAGTAGTTGGGGCTGATGGCGCTACCGCTGGGTTGATATGCTGCGAAAATCACTTAATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGAATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGAGCGCAGATCCCAGGAATGCCcaacacc                                                                                                                                                                                                                             >  1:149968/1‑249 (MQ=255)               aaaCACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCAAACACCGCGACGCCGGAAGAGAAGTAGTTGGTGATGATGGCGCTACCGCTGGTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGtt                                                                                                                                                                                                                         >  1:55416/1‑251 (MQ=255)                                                                  aaaCACCGCTACGCCGGAAGAGAAGTAGTTGGGGATGATGGCTCTACCGCTGGTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTTCGCGGCGCGTAATCC‑ACCAAGACCAt                                                                                                                                                                     <  2:255208/251‑1 (MQ=255)                                                                          cgACGCCGGAAGAGAAGTAGTTGGTGATGATGGCGCTACCGCTGGTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATATCGGCGGCGGCATAGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCAAAGGTGACATTAACTGCTGCGCGGCGCGTACTCC‑ACCAAGACCATCGGTAAtt                                                                                                                                                             >  1:257856/1‑251 (MQ=255)                                                                                  gAAGAGAAGTAGTTGGTGATGATGGCGCTACCGCTGGTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACACCGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCC‑ACCAAGACCATCGGCAATTGAaataat                                                                                                                                                     >  2:253705/1‑251 (MQ=255)                                                                                      agaAGTAGTTGGTGATGATGGCGCTACCGCTGGTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCC‑ACCAAGACCATCGGTAATTGAAATAATcccc                                                                                                                                                 <  1:148019/251‑1 (MQ=255)                                                                                       gaAGTAGTTGGTGATGATGGCGCTCCCGCTGGTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCC‑ACCAAGACCATCGGTAATTGAAATAATCCCCa                                                                                                                                                <  1:80620/251‑1 (MQ=255)                                                                                                         ggCGCTACCGCTGGTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAAt                                                                                                                                                                                   <  1:203165/199‑1 (MQ=255)                                                                                                         ggCGCTACCGCTGGTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAAt                                                                                                                                                                                   >  2:203165/1‑199 (MQ=255)                                                                                                                               gcggcgAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCC‑ACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAaga                                                                                                         <  1:30518/250‑1 (MQ=255)                                                                                                                                   cgAAAATCACTTTATCGCGGTCGGTAATCTCACCCTCCGGCGCCAGCCCTTGTGTCATGCCGCTGGCGTCATCGGTGGGTTCCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAGTTCCCAGCAACTGTTTTATGACAGGGGTCATTACCTGCTGCTCGGCGCGTAATCC‑TCGAAGACGAGCGGTAATTGAAAGAAACCCCAGAGAGAGAATGACTTACGGTGCCAGCTCCAGCGCAAAGAGAAAg                                                                                                    <  1:259742/251‑1 (MQ=255)                                                                                                                                                           tAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCC‑ACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCAccc                                                                            >  1:144906/1‑251 (MQ=255)                                                                                                                                                              tttCAACTTCCTGAGCCAGCTCTTTGGCAATACCGGCGGCGGCCTAGGTGTTTTGAAGGTTAGCGATCAACGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCC‑AACAAGAACATCGGTAAATGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGCAAGCCATATTTAGCCCCCGCGCCAccaacg                                                                         >  2:163313/1‑247 (MQ=255)                                                                                                                                                                    cTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCC‑ACCAAGACCCTCGGTACTTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCCGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACccgcgccgcg                                                                    >  1:187756/1‑250 (MQ=255)                                                                                                                                                                             ccAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCC‑ACCACGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTAACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGACCCACCCGCGCCGCGAAATGAggtg                                                          >  1:114166/1‑251 (MQ=255)                                                                                                                                                                                    tttGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGAGCGTACTCCCACCA‑GACCATCGGTAATTGAAATAATCCCCAGCGAGAGAATGACTGACGGGGCCAGCTCCATCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGACGAGAAAAGAgggagtggcc                                                    >  2:196063/1‑242 (MQ=255)                                                                                                                                                                                                    gcggcATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCC‑ACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCat                                   <  2:144906/251‑2 (MQ=255)                                                                                                                                                                                                     cggcATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCC‑ACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGCGGCTCCGTTCGCACCAGGCAGt                                  >  1:103235/1‑251 (MQ=255)                                                                                                                                                                                                               gtgttGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCC‑ACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACg                        <  1:253705/248‑1 (MQ=255)                                                                                                                                                                                                                        gTTAGCGATCAGCGCCAGCGCGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGTCGCGTAATCC‑ACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGCGGTGGTGGCTCCGTTCGCACCAGGCAGTGGCCCAAACGTTCCGTTCa               <  2:236793/251‑1 (MQ=255)                                                                                                                                                                                                                               aTCAGCGCCAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCC‑ACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGa        <  1:212255/251‑1 (MQ=255)                                                                                                                                                                                                                                      ccAGCGAGCAGATCCCAGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCC‑ACCAAGACCATCTGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATc  >  1:224710/1‑250 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                           GCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCAAACACCGCGACGCCGGAAGAGAAGTAGTTGGTGATGATGGCGCTACCGCTGGTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCC‑ACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATC  >  NC_000913/4560064‑4560542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑