Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,553,609	T→A	L237I (TTA→ATA)	uxuB →	D‑mannonate oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,553,609	0	T	A	98.0%	151.4 / NA	49	L237I (TTA→ATA)	uxuB	D‑mannonate oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (26/22);  minor base C (1/0);  total (27/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTCGGTTACCTCGGCGGCTATGAAACCATTGCCGACACCGTG  >  NC_000913/4553373‑4553854                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                      cGAAGTCCGCGATTGGTTACATCGTCGAAACGTTGCGTCTGCGTAGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGACAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGCAGAAAATGTCACCTTCCAGTGCACCATGGTTGACCGCATCGTTACGGCGGCGACGACAGAAACCATACAGGAAATTGCt                                                                                                                                                                                                                                         >  2:160344/1‑251 (MQ=255)    aGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTGAc                                                                                                                                                                                                                                      <  1:264669/251‑1 (MQ=255)     gTCCGCGATTGGTTACATCGTCGCAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTGCAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCCCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGGTCAGGCGGCGACGCCAGAAACCAAACAGGAAATTGCTGAcc                                                                                                                                                                                                                                     >  2:157847/1‑251 (MQ=255)                    cATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTGACCAGCTGGGTGTTTACg                                                                                                                                                                                                                      <  2:74347/251‑1 (MQ=255)                              gCGTTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTGCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATTCAGGAAATTGCTGACCAGCTGGGGGTTTACGACCCGTgcgc                                                                                                                                                                                                            <  2:108309/251‑1 (MQ=255)                                gTTGCGTCTGCGTCGTGACAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGcc                                                                                                                                                                                                           >  1:103081/1‑250 (MQ=255)                                    cgtctgcgtcGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGc                                                                                                                                                                                                      <  1:69590/251‑1 (MQ=255)                                          cgtcgtGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTGACCAGCTGGGGGTTTACGACCCGTGCGCCATTGCCTGCg                                                                                                                                                                                                 >  1:21548/1‑250 (MQ=255)                                           gtcgtGACAAAGGGTTGATAGCGTTTACGGAGATGTCATGCGATAACGTGCGTGAAAACGGTCTTGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGCCCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGctg                                                                                                                                                                                                >  2:111155/1‑248 (MQ=255)                                             cgtGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTGACCAGCTGGGTGTTTCCGACCCGTGGGCAATTGCCTgaggaac                                                                                                                                                                                             >  2:198154/1‑245 (MQ=255)                                                  aaaaGGGTTGAAAGCGTTTACGGTGATGTCCTGCGCTCACGTGCGTGACAACGCTCATGTGGCGAAGGTCGCGGTACTGGGCCTGGCTCAGACGCGTGACCCGAAGCTGGCGGCATGGACTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTGCCCAGCTGGGGGGTTACGACCCGTGCGCCCTTGCCTGCGAACCGTTcc                                                                                                                                                                                        >  2:141155/1‑251 (MQ=255)                                                   aaaGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTTATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCg                                                                                                                                                                                       <  2:4537/251‑1 (MQ=255)                                                     aGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGCAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTc                                                                                                                                                                                     >  2:140336/1‑251 (MQ=255)                                                          tGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCAGTg                                                                                                                                                                                 <  1:109658/250‑1 (MQ=255)                                                             aaGCGTTTGCGGTGATGTCCTGCGATAACGTGCGTGAAAGCGGTCATGTGGCGAAGGTCGCGGTACTGGGGCGGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCAGTGGGTg                                                                                                                                                                             <  1:124170/251‑1 (MQ=255)                                                                 gTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCAGTGGGTGATTg                                                                                                                                                                         <  1:162970/251‑1 (MQ=255)                                                                                            gcgtgAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCATGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCg                                                                                                                                               <  1:138165/250‑1 (MQ=255)                                                                                                 aaaaCGGTAATGTGGCGATGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGAGGAATGGCTTGAAGAAAATGTCACCTTCACGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTGACCCGCTGGGTGTTTACGACCCGTGAGCCATTGCCTGCGAACCGTTCCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTgg                                                                                                                                         >  2:235733/1‑251 (MQ=255)                                                                                                            gtgtcGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCCCCGTCCCGTGCACCAGGTTTGACCGCAGCGTTCCGGCGGCGACGCCAGAAACCAGACAGTAGATTGCTGACCAGCTGGGTGTGTACGACCCGTGCGCCATTTCCTGCGAGCCGTTCCGTCAGTTGGTGAGTGAAGATATCTTCGTTAATGGGCGCCCGGATTGGGATAAAgggg                                                                                                                               <  2:27692/246‑4 (MQ=255)                                                                                                                     gTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGtt                                                                                                                     <  2:130057/251‑1 (MQ=255)                                                                                                                             aCTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCa                                                                                                              <  2:103081/250‑1 (MQ=255)                                                                                                                                ggggCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGTCGGCGACGCCAGAAACCATACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACg                                                                                                          <  1:267288/251‑1 (MQ=255)                                                                                                                                      ggctCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTtgt                                                                                                      <  2:98108/249‑1 (MQ=255)                                                                                                                                                    aCCCGCAGCTGGCGGCATGGATTGACGAAAATGTCGCCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGACCCCCTACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACAGTTCCGCCAGTGGGTGGTTGACGATAACTTCGTTGATGGTCGCCCGGATTGGGATAAAGTGGGAGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAatgatg                                                                                      >  2:7042/1‑251 (MQ=255)                                                                                                                                                            cTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCg                                                                              >  2:94340/1‑251 (MQ=255)                                                                                                                                                             tGGCGGCTTGGATTACAGAAAAAGTCCCCTTCCCGTGCACCAGGGTTGACCGCCTCGCTCCGGCGGCGACGCCAGGAACCATACAGGAACTTGCTGACCAGCTGGGTGTTTACGACCCGTGAGCCATTGCCTGCGAATCGTTCCGTAAGGGGGTGGTTGAAGATAACTTCGTTACTGGTCGCCCGGATTGGGATAAAGTGGGCGCACCGTTCGTTGCAGACGTTGTGCCGTTCGCAATGATGACGCTGCGt                                                                             >  2:242457/1‑251 (MQ=255)                                                                                                                                                                 ggcATGGCTCGAAGAAAATCTCACCTTCCCGCGCAACATGGTTGAGCACATCGTTCCGGCGCCGACGCACGGAACAATACAGGACATTGCTGAACAGCTGGGTGTCTACGACCCGTGCGCCATTGCCCGCGAACCGTTCCGTCAGTGGGTGAATGAAGATAACTTCGTTAACGGTCGCCCGGATTGGGATAAAGTGGGCGCAACGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGc                                                                         >  2:20405/1‑251 (MQ=255)                                                                                                                                                                        aTTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCTCCATTGCCTGCGAACCGTTCCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCCGAACgg                                                                  <  2:30437/251‑1 (MQ=255)                                                                                                                                                                          tGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGc                                                                 >  1:75075/1‑250 (MQ=255)                                                                                                                                                                                 aaTGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCAGTGGGTGATTGAAGATAACt                                                                                                                                                                <  1:190845/148‑1 (MQ=255)                                                                                                                                                                                 aaTGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCAGTGGGTGATTGAAGATAACt                                                                                                                                                                >  2:190845/1‑148 (MQ=255)                                                                                                                                                                                  aTGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCActct                                                        <  1:140336/251‑1 (MQ=255)                                                                                                                                                                                  aTGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCActct                                                        >  1:33046/1‑251 (MQ=255)                                                                                                                                                                                  aTGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCActct                                                        >  1:151967/1‑251 (MQ=255)                                                                                                                                                                                     tCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCACTCtttt                                                     >  2:43375/1‑251 (MQ=255)                                                                                                                                                                                      cACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCACTCtttt                                                     <  1:41411/250‑1 (MQ=255)                                                                                                                                                                                      cACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGAGAACCGTTCCGTCAGTGGGAGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGGTGTGCCGTTCGAAATAGTAACGCTGCGTATGCTTACCGGAAGCCACTCTTTTc                                                    >  2:222120/1‑251 (MQ=255)                                                                                                                                                                                       cccgtCCCGTGCACCATGGGTGACCGCATCGCTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCTATTGCCTGCGAACCGTTCCGTCAGTGGGTGACTGAAGATAACTTCGTTAAGGGTCGCCCGGATTGGTAGAAAGTGGGCGCACAGTTCGTTGCAGCCGTTGTGCCGTTCGCAATGATGAAGCTGCGTATGCTGAACGGCAGACTCTCTTTTCt                                                   <  2:88155/247‑1 (MQ=255)                                                                                                                                                                                       aCCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGCAGCTGCGTATGCTGAACGGCAGCCACTCTTTTCt                                                   >  2:141585/1‑251 (MQ=255)                                                                                                                                                                                              cGTGCACCATGGTTGACCGAATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCAGTGGGTGATTGAAGATCACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGCATGCTGAACGGCCGCCACTCTTTTCTGGCGTAc                                            >  2:105612/1‑251 (MQ=255)                                                                                                                                                                                                  cACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATAAAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTCg                                        >  1:167658/1‑251 (MQ=255)                                                                                                                                                                                                   aCCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTTCTGGCGTCCCTCgg                                       <  1:71551/251‑1 (MQ=255)                                                                                                                                                                                                      aTGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTGACCAGCTGGGTGTTTACGTCCCGTGCGCCATTGCCTGCGAACCGTTCCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTCGGtt                                     >  1:181783/1‑250 (MQ=255)                                                                                                                                                                                                      aTGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTCGGtt                                     >  2:79252/1‑250 (MQ=255)                                                                                                                                                                                                                     gTTCCGGCGGCGACGCCAGAAACCATACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGTGTATGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTCGGTTACCTCGGCGGCTATg                      >  2:194743/1‑250 (MQ=255)                                                                                                                                                                                                                                   gCCAGAAACCATACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTCGGTTACCTCGGCGGCTATGAAACCATTGCCGaca       <  1:94340/251‑1 (MQ=255)                                                                                                                                                                                                                                     cAGAAACCATACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTCGGTTACCTCGGCGGCTATGAAACCATTGCCGACAcc     <  2:75838/251‑1 (MQ=255)                                                                                                                                                                                                                                       gAAACCATACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTCGGTTACCTCGGCGGCTATGAAACCATTGCCGACACCGt   >  1:17396/1‑251 (MQ=255)                                                                                                                                                                                                                                         aaCCATACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAACGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTCGGTTACCTCGGCGGCTATGAAACCATTGCCGACACCGTg  >  2:92046/1‑250 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                      CGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTCGGTTACCTCGGCGGCTATGAAACCATTGCCGACACCGTG  >  NC_000913/4553373‑4553854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑