Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,540,111	A→G	intergenic (‑610/‑846)	nanC ← / → fimB	N‑acetylneuraminate outer membrane channel/Type 1 fimbriae regulatory protein FimB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,540,111	0	A	G	100.0%	46.1 / NA	15	intergenic (‑610/‑846)	nanC/fimB	N‑acetylneuraminate outer membrane channel/Type 1 fimbriae regulatory protein FimB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/5);  total (10/5)

TTTATACCTGTTATACCAGATCAAAAATCACGCAATCCATACAACAAAACCAGATTTGCAATTCGTGTCACAAAATATGTCGATCTTTTTCTAAGAGGAAGATGCCATGTGAAGCCAGACGAACACTTGCGGTGGTCTTCAAAAACTAAAGATCTTAGTTTAACTATTTGTTTTATAAATAATTTATTAAGAGTCTAAACAAGGGGAGCTTTGCAAGCTAACTCAGTGAGCTTGGTGAAAATCAGTGTTTACCCGCCATCAGGCTGAGCATAATTCTCATCATGAAATATGTTTCCTGGTTTGTGGCTTGTAACTGGTCACTTCTGAAGTCGATCTGGAGAGGCTTGTTGATGTTGGTGTTTTCAGGATGATGTTTCACTTAGTTTGTTTGCCGTATCGCCCGGCGAATGGCTGTGATTGAGGAAGGTTTA  >  NC_000913/4539926‑4540356                                                                                                                                                                                          |                                                                                                                                                                                                                                                      tttATACCTGTTATACCAGATCAAAAATCACGCAATCCATACAACAAAACCAGATTTGCAATTCGTGTCACAAAATATGTCGATCTTTTTCTAAGAGGAAGATGCCATGTGAAGCCAGACGAACACTTGCGGTGGTCTTCAAAAACTAAAGATCTTAGTTTAACTATTTGTTTTATAAATAATTTGTTTAGAGTCTAAACAAGGGGAGCTTTGCAAGCTAACTCAGTGAGCTTGGTGAAAATCAGTGttt                                                                                                                                                                                       >  2:146748/1‑250 (MQ=255)                                                     gATTTGCAATTCGTGTCACAAAATATGTCGATCTTTTTCTAAGAGGAAGATGCCATGTGAAGCCAGACGAACACTTGCGGTGGTCTTCAAAAACTAAAGATCTTAGTTTAACTATTTGTTTTATAAATAATTTGTTTAGAGTCTAAACAAGGGGAGCTTTGCAAGCTAACTCAGTGa                                                                                                                                                                                                            >  1:58630/1‑177 (MQ=255)                                                     gATTTGCAATTCGTGTCACAAAATATGTCGATCTTTTTCTAAGAGGAAGATGCCATGTGAAGCCAGACGAACACTTGCGGTGGTCTTCAAAAACTAAAGATCTTAGTTTAACTATTTGTTTTATAAATAATTTGTTTAGAGTCTAAACAAGGGGAGCTTTGCAAGCTAACTCAGTGa                                                                                                                                                                                                            <  2:58630/177‑1 (MQ=255)                                                                                                               gAAGCCAGACGAACACTTGCGGTGGTCTTCAAAAACTAAAGATCTTAGTTTAACTATTTGTTTTATAAATAATTTGTTTAGAGTCTAAACAAGGGGAGCTTTGCAAGCTAACTCAGTGAGCTTGGTGAAAATCAGTGTTTACCCGCCATCAGGCTGAGCATAATTCTCATCATGAAATATGTTTCCTGGTTTGTGGCTTGTAACTGGTCACTTCTGAAGTCGATCTGGAGAGGCTTGTTGATGTTGGTGtt                                                                        <  1:71370/251‑1 (MQ=255)                                                                                                                            catttGCGGTGTTCTTCAAAAACTAAAGTTCTTAGTTTAACTATTTGTTTTATAAATAATTTGTTTAGAGTCTAAACAAGGGGAGCTTTGCAAGCTAACTCAGTGAGCTTGGGGAAAATCATTGTTTCCCCGCCATCAGGCGGAGCATAATTCTCATCATGAAATATGTTTCCTGGTTTGTGGCTTGTAACTGGTCACTTCTGAAGTCGATCTGGAGAGGCTTGTTGATGTTGGTGTTTTCAGGATGATGt                                                           <  1:123839/248‑1 (MQ=255)                                                                                                                               ttGCGGTGGTCTTCAAAAACTAAAGATCTTAGTTTAACTATTTGTTTTATAAATAATTTGTTTAGAGTCTAAACAAGGGGAGCTTTGCAAGCTAACTCAGTGAGCTTGGTGAAAATCAGTGTTTACCCGCCATCAGGCTGAGCATAATtc                                                                                                                                                             >  1:197394/1‑150 (MQ=255)                                                                                                                               ttGCGGTGGTCTTCAAAAACTAAAGATCTTAGTTTAACTATTTGTTTTATAAATAATTTGTTTAGAGTCTAAACAAGGGGAGCTTTGCAAGCTAACTCAGTGAGCTTGGTGAAAATCAGTGTTTACCCGCCATCAGGCTGAGCATAATtc                                                                                                                                                             <  2:197394/150‑1 (MQ=255)                                                                                                                                                   tAAAGATCTTAGTTTAACTATTTGTTTTATAAATAATTTGTTTAGAGTCTAAACAAGGGCAGCTTTGCAAGCTAAATCAGTGAGCTTGCTGAAAATAAGTGTTTACCCGCCATCAGGCTGAGCATAATTCTCATCATGAAATATGTTTCCTGCTTTGTGGCTTGTAACCGGTCACTTCTGAAGTCGATCTGGAGAGGCTTGTTGATGTTGGTGTTTTCAGGATGATGTTTCTCTTAGTTTGATTGCCGTAt                                    >  1:15816/1‑251 (MQ=255)                                                                                                                                                      aGATCTTAGTTTCACTATTTGTTTTATAAATAATTTGTTTAGAGTCTAAACAAGGGGAGCTTTGCAAGCTAACTCAGTGAGCTTGGTGAAAATCAGTGTTTACCCGCCATCAGGCTGAGCATAATTCTCATCATGAAATATGTTTCCTGGTTTGTGGCTTGTAACTGGTCACTTCTGAAGTCGATCTGGAGAGGCTTGTTGATGTTGGTGTTTTCAGGATGATGTTTCACTTAGTTTGTTTGCCGTATCGc                                 >  1:81837/1‑251 (MQ=255)                                                                                                                                                                ttAACTATTTGTTTTATAAATAATTTGTTTAGAGTCTAAACAAGGGGAGCTTTGCAATCTAACTCAGTTAGCTTGGTGAAAATCAGTGTTTACCCGCCATCAGGCTGAGCATAATTCTCATCATGAAATATGTTTCCTGGTTTGTGGCTTGTAACTGGTCACTTCTGAATTCGATCTGGAGAGGCTTGTTGATGTTGGTGTTTTTAGGATGATGTTTCACTTAGCTTGTTTGTCGTATCGCCCGGCGagtg                       >  2:142135/1‑248 (MQ=255)                                                                                                                                                                      aTTTGTTTTATAAATAATTTGTTTAGAGTCTAAACAAGGGGATCTTTGCCAGCTAACTCAGTGAGCTTGGTGAAAATCAGTGTTTCCCCGCCATCAGGCTGCGCATAATTCTCATCATGAAATATGTTTCCTGGTTTGTGGCTTGTCACTGGTAACTTCTGAAGTCGATCTGGAGAGGCTTGTTGATGTTGGTGTTTTCAGGATGATGTTTCACTTAGTTTGTTTGCCGTATCGCCCGGCGAATGGCtgtg                 >  1:181231/1‑251 (MQ=255)                                                                                                                                                                            tttATAAATAATTTGTTTAGAGTCTAAACAAGGGGAGCTTTGCAAGCTAACTCAGTGAGCTTGGTGAAAATCAGTGTTTACCCGCCATCAGGCTGAGCATAATTCTCATCATGAAATATGTTTCCTGGTTTGTGGCTTGTAACTGGTCACTTCTGAAGTCGATCTGGAGAGGCTTGTTGATGTTGGTGGTTTCAGGATGATGTTTCACTTAGGTTGTTTGCCGTATCGCCCGGCGAAAGGCTGTGGTTggg           >  2:206394/1‑249 (MQ=255)                                                                                                                                                                                 aaataaTTTGTTTAGAGTCTAAACAAGGGGAGCTTTGCAAGCTAACTCAGTGAGCTTGGTGAAAATCAGTGTTTACCCGCCATCAGGCTGAGCATAATTCTCATCATGAAATATGTTTCCTGGTTTGTGGCTTGTAACTGGTCACTTCTGACGTCGATCTGGAGAGGCTTGTTGATGTTGgt                                                                           >  1:12815/1‑182 (MQ=255)                                                                                                                                                                                 aaataaTTGGTTTAGAGTCTAAACAAGGGGAGCTTGGCAAGCTAACTCAGTGATCTGGGTGAAAAGCAGTGTTTACCCGCCATCAGGCTGAGCATAATTCTCATCATGAAATATGTTTCCTGGTTTGTGGCTTGTAACTGGTCACTTCTGAAGTCGATCTGGAGAGGCTTGTTGATGTTGgt                                                                           <  2:12815/182‑1 (MQ=255)                                                                                                                                                                                     aatTTGTTTAGAGTCTAACCAAGGGGAGCTTTGCAAGCTAACTCAGTGAGCTTGGTGAAAATCAGTGTTTACCCGCCATCAGGCTGAGCATAATTCTCATCATGAAATATGTTTCCTGGTTTGTGGCTTGTAACTGGTCACTTCTGAAGTCGATCTGGAGAGGCTTGTTGATGTTGGGGTTTTCAGGATGATGTTTCCCTTAGTTTGTTTGCCGTATCGCCCGGCGAATTGCTGTGATTGAGGAAGGtttt  >  2:109004/1‑250 (MQ=255)                                                                                                                                                                                          |                                                                                                                                                                                                                                                      TTTATACCTGTTATACCAGATCAAAAATCACGCAATCCATACAACAAAACCAGATTTGCAATTCGTGTCACAAAATATGTCGATCTTTTTCTAAGAGGAAGATGCCATGTGAAGCCAGACGAACACTTGCGGTGGTCTTCAAAAACTAAAGATCTTAGTTTAACTATTTGTTTTATAAATAATTTATTAAGAGTCTAAACAAGGGGAGCTTTGCAAGCTAACTCAGTGAGCTTGGTGAAAATCAGTGTTTACCCGCCATCAGGCTGAGCATAATTCTCATCATGAAATATGTTTCCTGGTTTGTGGCTTGTAACTGGTCACTTCTGAAGTCGATCTGGAGAGGCTTGTTGATGTTGGTGTTTTCAGGATGATGTTTCACTTAGTTTGTTTGCCGTATCGCCCGGCGAATGGCTGTGATTGAGGAAGGTTTA  >  NC_000913/4539926‑4540356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑