Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,535,073	A→G	intergenic (‑1020/‑1524)	topAI ← / → ythB	toxin of the TopAI‑YjhQ toxin‑antitoxin system, TopA inhibitor/protein YthB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,535,073	0	A	G	100.0%	94.5 / NA	30	intergenic (‑1020/‑1524)	topAI/ythB	toxin of the TopAI‑YjhQ toxin‑antitoxin system, TopA inhibitor/protein YthB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/12);  total (18/12)

GGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAACATCATCGACTACTGTAACTCACTTTGCTATCACGGTAAGTTGCAGCCTAAAAGAGGGATGGAAAAAGGACGATTTTCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAACCCTCTTGAAGCTGAAACAATAGCAGCATGGCTGGTGGCACATAAGGATGATATAGAGCGCCATTACGGGGAACCACTGTACAAAGTTGTTGGTGTTGTGACGCCATTTTCGGCGCAGGTTAATGCTATCAAAATGTCATTGCGTAAGCTGGAAATTAATGGCAAAGACGAGCAGGGTTTACTGACAGTGGGGACTGTGCACTCCTTGCAGGGGGCGGAAAGGGCGATTG  >  NC_000913/4534830‑4535303                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                       ggCAGCGTAATGCAGGTAGCCCTGTTCAACTCTCATTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTAGCTGTATGAGCATCGTCGCTGTGTCAATAACATCATCGACTACTGTAACTCGCTTTGCTATCACGGTAAGTTGCAGCCTAAAAGAGGGATGGAAAAAGGACGATTTTCCCCGCAATGGGATATTTACATATTGATGATAGAGTCATGAATTCAAATGGAGGGAGCCGTCATAGcccc                                                                                                                                                                                                                                    <  2:171924/248‑2 (MQ=255)      cGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAACATCATCGACTACTGTAACTCACTTTGCTATCACGGTAAGTTGCAGCCTAAAAGAGGGATGGAAAAAGGACGATTTTCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAGCCCTCTTGAAGc                                                                                                                                                                                                                            >  1:82255/1‑251 (MQ=255)       gTAATGCAGATAGCCCTGTTCAACTCTCACTACCAGTATGATCCCAAGGTAGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAACATCATCGACTACTGTAACTCACTTTGCTAGCACGGTAAGTTGCAGCCTAAAAGAGGGATGGAAAAAGGACGATTTTCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAGCCATCTTGAAGCt                                                                                                                                                                                                                           >  2:194140/1‑251 (MQ=255)                  aGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAACATCATCGACTACTGTAACTCACTTTGCTATCACGGTAAGTTGCAGCCTAAAAGAGGGATGGAAAAAGGACGATTTTCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAGCCCTCTTGAAGCTGAAACAATagc                                                                                                                                                                                                                >  2:68471/1‑251 (MQ=255)                                      aCCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAACATCATCGACTACTGTAACTCACTTTGCTATCACGGTAAGTTGCAGCCTAAAAGAGGGATGGAAAAAGGACGATTTTCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGCCAAATGGAGGGGGCCGTCATAGCCCTCTTGAAGCTGAAACATTAGCAGCATGGCTGGTGGCACAt                                                                                                                                                                                             <  2:263818/250‑1 (MQ=255)                                            aTGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAACATCATCGACTACTGTAACTCACTTTGCTATCACGGTAAGTTGCAGCCTAAAAGAGGGATGGAAAAAGGACGATTTTCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAGCCCTCTTGAAGATGAAACAATAGCAGCATGGCTGGTGGCACATAAGgatg                                                                                                                                                                                      >  2:197206/1‑251 (MQ=255)                                              gATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAAGACCATCATCGACTACTGTAACTCACTTTGCTATCACGGTAAGCTGCAGCCTAAAAGAGGGTTGGAAAAAGGACGATTTTCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAGCCCTCTTGAAGCTGAAATCATAACAGCATGGCTGGTGGCACATAAGgatgat                                                                                                                                                                                    >  2:1722/1‑251 (MQ=255)                                                      gTTCGCGCGCGGAATGTATATGTATGAGCATCGTCGCTGCTTCAATAACCTCATCGACTACAGAAACTCACTTTGCTATCACGGTACGTTGCAGACTAAAAGAGGGATGGAAAACCGCCGATTTTCCCCGCAATGGGATATTTACATATTGATGGCAGAGGCATGAAGCCACATGGAACGAGCCGTCATAGACCTCTTGAAGCTGAACCAATAGCAGCATGGCTGGTGGCACATAAGGCTGATATagag                                                                                                                                                                              >  2:109908/1‑249 (MQ=255)                                                                              tGAGCATCGTCGCTGTGTCAATAACATCATCGACTACTGTAACTCACTTTGCTATCACGGTAAGTTGCAGCCTAAAAGAGGGATGGAAAAAGGACGATTTTCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAGCCCTCTTGAAGCTGAAACAATAGCAGCATGGCTGGTGGCACATAAGGATGATATAGAGCGCCATTACGGGGAATCACTGTACa                                                                                                                                                     <  2:258859/250‑1 (MQ=255)                                                                               gAGCATCGTCGCTGTTTCAATAACATCATCGACTACTGTAACTCACTTTGCTATCACGGTAAGTTGCAGCCTAAAAGAGGGATGGAAAAAGGACGATTTTCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAGCCCTCTTGAAGCTGAAACAATAGCAGCATGGCTGGTGGCACATAAGGATGATATAGAGCGCCATTACGGGGAACCACTGTACaa                                                                                                                                                    <  2:53311/250‑1 (MQ=255)                                                                                aGCATCGTCGCTGTTTCAATAACATCATCGACTACTGTAACTCACTTTGCTATCACGGTAAGTTGCAGCCTAAAAGAGGGATGGAAAAAGGACGATTTTCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAGCCCTCTTGAAGCTGAAACAATAGCAGCATGGCTGGTGGCACATAAGGATGATATAGAGCGCCATTACGGGGAACCACTGTACAAAg                                                                                                                                                  <  1:137108/251‑1 (MQ=255)                                                                                 gCATCGTCGCTGTTTCAATAACATCATCGACTACTGTAACTCACTTTGCTATCACGGTAAGTTGCAGCCTAAAAGAGGGATGGAAAAAGGACGATTTTCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAGCCCTCTTGAAGCTGAAACAATAGCAGCATGGCTGGTGGCACATAAGGATGATATAGAGCGCCATTACGGGGAACCACTGTACAAAgt                                                                                                                                                 >  1:60444/1‑251 (MQ=255)                                                                                         gCTGTTTCAATAACCTCATCGCCTACTGTAACTCACTTTGCTATCACGGTAAGTTGCAGCCTAAACGAGAAATGGAAACAGGACGATTTTCACCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGACAAATGGAGGGAGCCGTCATAGCCCTCTTGAAGCTGAAACAATAGCAGCATGGCTGGTGGCACATAAGGATGATATAGAGCGCCCTTACGGGGAACCACTGTACAAAGTTGTTGgtg                                                                                                                                         >  2:43301/1‑251 (MQ=255)                                                                                            gTTTCAATAACATCATCGACTACTGTAACTCACTTTGCTATCACGGTAAGTTGCAGCCTAAAAGAGGGATGGAAAAAGGACGATTTTCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAGCCCTCTTGAAGCTGAAACAATAGCAGCATGGCTGGTGGCACATAAGGATGATATAGAGCGCCATTACGGGGAACCACTGTACAAAGTTGTTGgtgt                                                                                                                                        <  2:82255/249‑1 (MQ=255)                                                                                                 aaTAACATCATCGACTACTGTAACTCACTTTGCTATCACGGTAAGTTGCAGCCTAAAAGAGGGATGGAAAAAGGACGATTTTCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAGCCCTCTTGAAGCTGAAACAATAGCAGCATGGCTGGTGGCACATAAGGATGATATAGAGCGCCATTACGGGGAACCACTGTACAAAGTTGTTGGTGTTGTGACg                                                                                                                                 >  1:147757/1‑251 (MQ=255)                                                                                                        tcatcGACTACTGTAACTCACTTTGCTATCACGGTAAGTTGCAGCCTAAAAGAGGGATGGAAAAAGGACGATTTTCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAGCCCTCTTGaa                                                                                                                                                                                                                              >  2:11782/1‑151 (MQ=255)                                                                                                        tcatcGACTACTGTAACTCACTTTGCTATCACGGTAAGTTGCAGCCTAAAAGAGGGATGGAAAAAGGACGATTTTCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAGCCCTCTTGaa                                                                                                                                                                                                                              <  1:11782/151‑1 (MQ=255)                                                                                                         catcGACTACTGTAACTCACTTTGCTATCACGGTAAGTTGCAGCCTAAAAGAGGGATGGAAAAAGGACGATTTTCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAGCCCTCTTGAAGCTGAAACAATAGCAGCATGGCTGGTGGCACATAAGGATGATATAGAGCGCCATTACGGGGAACCACTGTACAAAGTTGTTGGTGTTGTGACGCCATTTTc                                                                                                                         <  2:152216/251‑1 (MQ=255)                                                                                                          atcGACTACTGTAACTCACTTTGCTATCACGGTAAGTTGCAGCCTAAAAGAGGGATGGAAAAAGGACGATTTTCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAGCCCTCTTGAAGCTGAAACAATAGCAGCATGGCTGGTGGCACATAAGGATGATATAGAGCGCCATTACGGGGAACCACTGTACAAAGTTGTTGGTGTTGTGACGCCATTTTCg                                                                                                                        >  2:93731/1‑251 (MQ=255)                                                                                                            cGACTACTGTAACTCACTTTGCTATCACGGTAAGTTGCAGCCTAAAAGAGGGATGGAAAAAGGACGATTTTCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAGCCCTCTTGAAGCTGAAACAATAGCAGCATGGCTGGTGGCACATAAGGATGATATAGAGCGCCATTACGGGGAACCACTGTACAAAGTTGTTGGTGTTGTGACGCCATTTTCGgc                                                                                                                      <  2:72662/251‑1 (MQ=255)                                                                                                            cGACTACTGTAACTCAATTTGCTATCACGGTAAGTTGCAGCCTAAAAGAGGGATGGCAAAAGGACGCTTTTCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAGCCCTCTTGAAGCTGAAACAATAGCAGCATGGCTGGTGGCACATAAGGATGATATAGAGCGCCATTACGGGGAACCACTGTACAAAGTTGTTGGTGTTGGGACGCCATTTTCGgc                                                                                                                      >  2:125394/1‑251 (MQ=255)                                                                                                             gACTACTGTAACTCACTTTGCTATCACGGTAAGTTGCAGCCTAAAAGAGGGATGGAAAAAGGACGATTTTCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAGCCCTCTTGAAGCTGAAACAATAGCAGCATGGCTGGTGGCACATAAGGATGATATAGAGCGCCATTACGGGGAACCACTGTACAAAGTTGTTGGTGTTGTGACGCCATTTTCGgcg                                                                                                                     <  1:93731/251‑1 (MQ=255)                                                                                                             gACTACTGTAACTCACTTTGATATCCCGGTAAGTTGCAGCCTAAAAGAGGGATGGAAAAAGGACGATTTTCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAGCCCTCTTGAAGCTGAAACAATAGCAGCATGGCTGGTGGCACATAAGGATGATATAGAGCGCCATTACGGGGAACCACTGTACAAAGTTGTTGGTGTTGTGACGCCATTTTCGgcg                                                                                                                     >  2:266926/1‑251 (MQ=255)                                                                                                                                       cGGTAAGATGCAGCCTTAAAGAGGGATGGAAAAAGGACGATTTTCCCCGTAATGGGATATTTATATATTGATGGTAGAGGCATTAAGCCAAAAGGAGGGAGTCGTCATAGCCCTCTTGAAGCTGAAACAATAGCAGCATGTCTGGTGGCACATAAGGATGATATAGAGCGCCATTACGTTGAACCACTGTACAAAGTTGTTGGTGTTGTGACCCCCTTTTCGGCGTAGGTTAATTCTATCAAACTGTCa                                                                                             <  1:129627/249‑1 (MQ=255)                                                                                                                                                       aaaaGAGGGATGGAAAAAGGACGATTTTCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAGCCCTCTTGAAGCTGAAACAATAGCAGCATGGCTGGTGGCACATAAGGATGATATAGAGCGCCATTACggg                                                                                                                                                                  <  1:91404/164‑1 (MQ=255)                                                                                                                                                       aaaaGAGGGATGGAAAAAGGACGATTTTCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAGCCCTCTTGAAGCTGAAACAATAGCAGCATGGCTGGTGGCACATAAGGATGATATAGAGCGCCATTACggg                                                                                                                                                                  >  2:91404/1‑164 (MQ=255)                                                                                                                                                         aaGAGGGATGGAAAAAGGACGATTTTCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAGCCCTCTTGAAGCTGAAACAATAGCAGCATGGCTGGTGGCACATAAGGATGATATAGAGCGCCATTACGGGGAACCACTGTACAAAGTTGTTGGTGTTGTGACGCCATTTTCGGCGTAGGTTAATGCTATCAAAATGTCATTGCGTAAGCTGGAAGTTa                                                                          >  1:150258/1‑250 (MQ=255)                                                                                                                                                          agagGGATGGAAAAAGGACGATTTTCCCCGCAATGGGATATTTACATATTGATGGTAGAGGAATGAAGCCAAATGGAGGGAGCCGTCATAGCCCTCTTGAAGCTGCAACAATAGCAGCATGGCTGGTGGCACATAAGGATGATATAGAGCGCCATTACGGGGAACCACTGTACAAAGTTGTTGGTGTTGTGACGCCATTTTCGGCGTCGGTTAATGCTATCAATATGTCATTGCGTAAGCTGGAAATTAAt                                                                        >  2:138143/1‑251 (MQ=255)                                                                                                                                                                        aGGACGATTTTCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAGCCCTCTTGAAGCTGAAACAATAGCAGCATGGCTGGTGGCACATAAGGATGATATAGAGCGCCATTACGGGGAACCACTGTACAAAGTTGTTGGTGTTGTGACGCCATTTTCGGCGTAGGTTAATGCTATCAAAATGTCATTGCGTAAGCTGGAAATTAATGGCAAAGACGAGc                                                           >  2:113373/1‑250 (MQ=255)                                                                                                                                                                                                                                aaaTGGAGGGAGCCGTCATAGCCCTCTTGAAGCTGAAACAATAGCAGCATGGCTGGTGGCACATAAGGATGATATAGAGCGCCATTACGGGGAACCACTGTACAAAGTTGTTGGTGTTGTGACGCCATTTTCGGCGTAGGTTAATGCTATCAAAATGTCATTGCGTAAGCTGGAAATTAATGGCAAAGACGAGCAGGGTTTACTGACAGTGGGGACTGTGCACTCCTTGCAGGGGGCGGAAAGGGCGATTg  >  1:33389/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                       GGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAACATCATCGACTACTGTAACTCACTTTGCTATCACGGTAAGTTGCAGCCTAAAAGAGGGATGGAAAAAGGACGATTTTCCCCGCAATGGGATATTTACATATTGATGGTAGAGGCATGAAGCCAAATGGAGGGAGCCGTCATAACCCTCTTGAAGCTGAAACAATAGCAGCATGGCTGGTGGCACATAAGGATGATATAGAGCGCCATTACGGGGAACCACTGTACAAAGTTGTTGGTGTTGTGACGCCATTTTCGGCGCAGGTTAATGCTATCAAAATGTCATTGCGTAAGCTGGAAATTAATGGCAAAGACGAGCAGGGTTTACTGACAGTGGGGACTGTGCACTCCTTGCAGGGGGCGGAAAGGGCGATTG  >  NC_000913/4534830‑4535303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑