Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,532,063	C→T	intergenic (‑412/+374)	sgcX ← / ← yjhP	putative endoglucanase with Zn‑dependent exopeptidase domain/putative methyltransferase YjhP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,532,063	0	C	T	100.0%	91.5 / NA	30	intergenic (‑412/+374)	sgcX/yjhP	putative endoglucanase with Zn‑dependent exopeptidase domain/putative methyltransferase YjhP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/14);  total (16/14)

GCTGGAGAGACCAGTGATTATGGTTGAAATAATGAGGATGAATAAAACGCGGTAACTGTTATAGCACCACTTTGTTGCAGGACGCTCCCTCTTCATTACGTCCTGCACGTGGATACCCCAGCATCTCCATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCACTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTG  >  NC_000913/4531817‑4532306                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    gCTGGAGAGACCAGTGATTATGGTTGAAATAATGAGGATGAATAAAACGCGGTAACTGTTATAGCACCACTTTGTTGCAGGACGCTCCCTCTTCATTACGTCCTGCACGTGGATACCTCAGCATCTCCATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATtctc                                                                                                                                                                                                                                                 <  1:210840/251‑1 (MQ=255)      agagaCCAGTGATTATGGTTGAAATAATGAGGATGAATAAAACGCGGTAACTGTTATAGCACCACTTTGTTGCAGGACGCTCCCTCTTCATTACGTCCTGCACGTGGATACCCCAGCATCTCCATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTAAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCtt                                                                                                                                                                                                                                            >  1:227147/1‑251 (MQ=255)      agagaCCAGTGATTATGGTTGAAATAATGAGGATGAATAAAACGCGGTAACTGTTATAGCACCACTTTGTTGCAGGACGCGCCCTCTTCATTACGTCCTGCACGTGGATACCCCAGCATCTCCATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTACCCAGCATAATGTAAGAGAACATCGGATCTCCTGAAGAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCtt                                                                                                                                                                                                                                            <  1:114588/251‑1 (MQ=255)                   tATGGGTGAAATAATGAGGATGAATAAAACGCGGTAACTGTTGTAGCACCACTTTGTTGCAGGACGCTCCCTCTTCATTACGTCCTGCACGTGGATACCCCAGCATCTCCATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCGCTGCTCTATGAGCCCAgg                                                                                                                                                                                                                                <  2:3797/250‑1 (MQ=255)                                  gAGGATGAATAAAACGCGGTAACTGTTATAGCACCCCTTTGTTGCAGGACGCTCCCTCTTCATTACGTCCTGCACGTGGATACCCCAGCATCTCCATCAGCGGCTCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCTGTc                                                                                                                                                                                                                  >  2:225208/1‑249 (MQ=255)                                                   cgTAACCGTTATAGCGCCTCTTTGTTGCAGGCCGCGCCCTCTTCATTACGTCCTGCACGTGGATGCCCCAGCATCGCCATCAGCGGATCGTAGAATGTAATTGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGAGCTCCTGAATAGATTCAGAGGTAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCt                                                                                                                                                                                               <  2:88776/250‑1 (MQ=255)                                                      aaCTGTTATAGCACCACTTTGTTGCAGGACGCTCCCTCTTCATTACGTCCTGCACGTGGATACCCCAGCATCTCCATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTTAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTAt                                                                                                                                                                                            >  2:45665/1‑251 (MQ=255)                                                                   ccacTTTGTTGCAGGACGCTCCCTCTTCAGTACGTCCTGCACGTGGATACCCCAGCATCTCCATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAGCGTTTTGCACGCTTATGTGCTTCCTCCgg                                                                                                                                                                               <  2:152542/251‑1 (MQ=255)                                                                        ttgttgCAGGACGCTCCCTCTTCATTACGTCCTGCACGTGGATACCCCAGCATCGCCATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGcc                                                                                                                                                                            <  1:142985/249‑1 (MQ=255)                                                                              cagaaCGCTCCCTCTTCATTACGTCCTGCACGTGGATACCCCAGCATATCCATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGACAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGATGGCATTGCCGTCAGATAACGTTTTTCACGCTTATGTGCTTCCTCCGGGCCGACttttt                                                                                                                                                                    >  2:162122/5‑251 (MQ=255)                                                                                   cGCTCCCTCGTCATTACGTCCTGCACGTGGATACCCCAGCATCTCCATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTTAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAg                                                                                                                                                               <  1:45665/251‑1 (MQ=255)                                                                                         ctctTCATTACGTCCTGCACGTGGATACCCCAGCATCTCCATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATc                                                                                                                                                          >  1:188392/1‑250 (MQ=255)                                                                                             tCATTACGTCCTGCACGTGGATACCCCAGCATCTCCATCAGCGTATCTTAGAATGTAATGGCGCGTGGCAGGTCTTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCGTTATGAGCCCAGGCTGTCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAgg                                                                                                                                                      <  1:240912/250‑1 (MQ=255)                                                                                                                      ccAGCATCTCCATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTACGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATAATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGcttc                                                                                                                            >  1:167317/1‑251 (MQ=255)                                                                                                                                        gATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGa                                                                                                                                                     >  1:11685/1‑208 (MQ=255)                                                                                                                                        gATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGa                                                                                                                                                     <  2:11685/208‑1 (MQ=255)                                                                                                                                                                  aGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTGAGATCGCCGTTGCTGTCGCTGCATTACGcac                                                                                >  1:225581/1‑251 (MQ=255)                                                                                                                                                                                 aGCATAATGTAAGAAAACAACGGATCTCCTGAATAGAATCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCTCAATATTGATCACTgg                                                                 <  2:163336/251‑1 (MQ=255)                                                                                                                                                                                                  cATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTACTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTTAGATTGCCGTTGCTGTCGCTGCATTACGCGCAATATTGAGCACTGGCACTGTGTGCATCTGGt                                                <  2:132044/251‑1 (MQ=255)                                                                                                                                                                                                     cGGATCTCCTGAATAGAGTCAGAGGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCGGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGGGTGCATCTGGTGc                                              <  2:188392/250‑1 (MQ=255)                                                                                                                                                                                                                   aGATTCAGATGGAGTATAGATAATCAGCTCAAATCATTCTCCTCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGCTGCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTc                                <  2:201637/250‑1 (MQ=255)                                                                                                                                                                                                                         agagGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGc                         >  2:170025/1‑251 (MQ=255)                                                                                                                                                                                                                         agagGGAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGc                         >  2:215517/1‑251 (MQ=255)                                                                                                                                                                                                                            gggAGTATAGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTg                      >  1:197423/1‑251 (MQ=255)                                                                                                                                                                                                                                  ataGATAATCACCTCACATCATTCTACGATTTATGAGCCCCGGCTGGCAATGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTACGGGACGCCTTTTTACCAGGAATCAAGGAAGCTGGCTAAACCTGATACAGCTCCTTCCCAGGGTTTCAGATTGCCGTTGCTGTCGCTGCATTACGCACACTATTGATCACTGGCCCTGTGTGCATATGGTGCCGACATCGGCATTCCGCAAGCCTGAccga                 >  2:259285/1‑247 (MQ=255)                                                                                                                                                                                                                                    aGATAATCAGCTCAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACt              >  2:144046/1‑251 (MQ=255)                                                                                                                                                                                                                                         aTCAGATCAACTCATTCTACGCTTTATGAGCCCACGCTGGCATTGCCGTCAGATAACGTTTTGCACTCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGCTACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCCAGCCTGACAGAACTGTTc          >  2:46798/1‑250 (MQ=255)                                                                                                                                                                                                                                                cAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTgg                                                 >  1:80818/1‑204 (MQ=255)                                                                                                                                                                                                                                                cAAATCATTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTgg                                                 <  2:80818/204‑1 (MQ=255)                                                                                                                                                                                                                                                cAAATCATTCTCCGCTTTATGAGACCAGGCTGGAATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTtgtg  >  1:76005/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    GCTGGAGAGACCAGTGATTATGGTTGAAATAATGAGGATGAATAAAACGCGGTAACTGTTATAGCACCACTTTGTTGCAGGACGCTCCCTCTTCATTACGTCCTGCACGTGGATACCCCAGCATCTCCATCAGCGGATCTTAGAATGTAATGGCGCGTGGCAGGTCGTTGGTTCCCAGCATAATGTAAGAAAACATCGGATCTCCTGAATAGATTCAGAGGGAGTATAGATAATCAGCTCAAATCACTCTCCGCTTTATGAGCCCAGGCTGGCATTGCCGTCAGATAACGTTTTGCACGCTTATGTGCTTCCTCCGGGCCGACTTTTTGCCAGGAATCAAGGAAGCTGGCTAAACCTGATACCGCTTCTTCCCAGGGTTTGAGATTGCCGTTGCTGTCGCTGCATTACGCACAATATTGATCACTGGCACTGTGTGCATCTGGTGCCGACATCGGCATTCCGCAAGCCTGACAGAACTGTTCATGTTGTG  >  NC_000913/4531817‑4532306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑