Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,516,632	T→C	T16A (ACC→GCC)	fecA ←	ferric citrate outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,516,632	0	T	C	100.0%	193.2 / NA	62	T16A (ACC→GCC)	fecA	ferric citrate outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/40);  total (22/40)

AGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGTGTTAACCAAAGGTGTTGTTTTACGAAAAACGCGTAACGGCGTCATACCTTCCCCATCATCATTTTTGTTGTGTTCAGCTATGAGTCGAACGAGAAGGGTAAAAAGGACAATCGAAATAAGAATTATTTTCCTTACAGTGGTGAAATGTTTATCCAGTACCGCGTAATAGACTGAATTTTAACGGGAAGCGTTTGCGCGATAACGTTCAGGATCGCATCGGTATTTTT  >  NC_000913/4516388‑4516859                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                    aGCGGTTTTCCCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAAc                                                                                                                                                                                                                               <  1:76545/251‑1 (MQ=255)    ggTTTTCCCTGCAGTCCTCTGCCGTCCAGCAGTTGTGGCATGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCTCGCGTCAGGCTTGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCCAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCaa                                                                                                                                                                                                                            <  1:43062/251‑1 (MQ=255)           ccTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCAAAGGtgtt                                                                                                                                                                                                                     >  2:8083/1‑251 (MQ=255)              gCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTTACCAAAGGTgttgtt                                                                                                                                                                                                                  <  1:196688/251‑1 (MQ=255)              gCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCAAAGGtgttgt                                                                                                                                                                                                                   <  1:66273/250‑1 (MQ=255)               cggtccgtggccgaccagcgtttgttgCAGGCCGCCCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGGCAACCGAGAGGGTAAATCCGCTGGGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCTAATGGCGTTAACCAAAGGGGTTGttt                                                                                                                                                                                                                 <  2:120677/230‑1 (MQ=255)                 gTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCAAAGGTGTTGtttt                                                                                                                                                                                                                >  1:94471/1‑250 (MQ=255)                           gTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCAAAGGTGTTGTTTTACGAAAAAcgc                                                                                                                                                                                                     >  1:207790/1‑251 (MQ=255)                           gTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCAAAGGTGTTGTTTTACGAAAAAcgc                                                                                                                                                                                                     <  1:225392/251‑1 (MQ=255)                                 cagTTGTTGCAGGCCGCTCTCGACTCCGTAGTCGCCGTGCTGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCTAGCTGCAGCAGGCTCAGTCGAATGGCGTTAACCAAAGGTGTTGTTTTACGACATACGCGTAACg                                                                                                                                                                                               <  2:255139/251‑1 (MQ=255)                                        tgCAGGCAGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGCCGTTAACCAAAGGTGTTGTTTTACGAAAAACGCGGAAGGGCGTCAt                                                                                                                                                                                        >  1:31394/1‑251 (MQ=255)                                          cAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCAAAGGTGTTGTTTTACGAAAAACGCGTAACGGCGTCATAc                                                                                                                                                                                      >  1:233446/1‑251 (MQ=255)                                          cAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGGGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCAAAGGTGTTGTTTTACGAAAAACGCGTAACGGCGTCATAc                                                                                                                                                                                      >  2:131591/1‑251 (MQ=255)                                            ggCCGCTCTCGACGTCGTAAGCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCAAAGGTGTTGTTTTACGAAAAACGCGTAACGGCGTCATACCt                                                                                                                                                                                    <  2:67238/251‑1 (MQ=255)                                              ccGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCAAAGGTGTTGTTTTACGAAAAACGCGTAACGGCGTCATACATTc                                                                                                                                                                                  >  1:58434/1‑251 (MQ=255)                                                  tctcGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATAGAGCGATCCCGGTGCGATATTAACCTGTGAAGCAAAAGAGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCACAGGCGAATGGCGTTAACCAAAGGTGTTGTTTTCCGAAAAACGCGTCACGGCGTCATACCTTcccc                                                                                                                                                                               >  1:179352/1‑250 (MQ=255)                                                   cccGACGTCGTAATCGCCGTGCAGGCCGTTGCGCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATAGTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCAAAGGTGTTGGTTTACGAAAAACGCGTAAGGGCGTCATACCTGCCCcat                                                                                                                                                                             <  2:45812/249‑1 (MQ=255)                                                     cGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCAAAGGTGTTGTTTTACGAAAAACGCGTAACGGCGTCATACCTTCCCcatca                                                                                                                                                                           <  2:7281/251‑1 (MQ=255)                                                      gACGTCGTAATCGCCGTGTAGGACGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGCAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCAAAGGGGTTGTTTTACGAAAAACGCGTAACGGCGTCATACCTTCCCcatcat                                                                                                                                                                          >  1:128009/1‑251 (MQ=255)                                                        cgtcgtAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGCGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATCTTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCAAAGGTGTTGTTTTACGAAAAACGCGTAACGGCGTCATACCTTCCCcatcatc                                                                                                                                                                         >  1:266486/1‑250 (MQ=255)                                                         gtcgtAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCAAAGGTGTTGTTTTACGAAAAACGCGTAACGGCGTCATACCTTCCCcatcatcat                                                                                                                                                                       >  2:38541/1‑251 (MQ=255)                                                           cgtAATCGCTGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCAAAGGTGTTGTTTTACGAAAAACGCGTAACGGCGTCATACCTTCCCCAGCATCAtt                                                                                                                                                                      <  1:222283/250‑1 (MQ=255)                                                                     gttcAGGGCGTGGCTCTGCTTGCCGTGCTGCAGGCTGGCGTCAACCGAGAGGGTAATTCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGATACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCAAAGGTGTTGTTTTACGAAAAACGCGTAACGGCGTCATACCTTCCCCATCATCAGTTTTGTtgtg                                                                                                                                                             <  1:220374/246‑1 (MQ=255)                                                                           gCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCAGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCAACGGTGTTGTTTTACGAAAAACGCGTAACGGCGTCATACCTTCCCCATCATCATTTTTGTTGTGTTCAGCt                                                                                                                                                      >  2:41386/1‑250 (MQ=255)                                                                                 gCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCAAAGGTGTTGTTTTACGAAAAACGCGTAACGGCGTCATACCTTCCCCATCATCATTTTTGTTGTGTTCAGCTATGAGTc                                                                                                                                               <  1:121257/251‑1 (MQ=255)                                                                                    ctGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCAAAGGTGTTGTTTTACGAAAAACGCGTAACGGCGTCATACCTTCCCCATCATCATTTTTGTTGTGTTCAGCTATGAGTCg                                                                                                                                              <  1:46894/249‑1 (MQ=255)                                                                                    ctGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCAAAGGTGTTGTTTTACGAAAAACGCGTAACGGCGTCATACCTTCCCCATCATCATTTTTGTTGTGTTCAGCTATGAGTCg                                                                                                                                              <  2:70008/249‑1 (MQ=255)                                                                                             gcgccTCAGGCGTGCGTCAACCGAGAGGGTAAAGCCGCTGTGTGCGGCATTCTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCGGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATTGCGTTAACCAAAGGTGTTGTTTTACGAAAAACGCGTAACGGCGTCATACCTTCCCCATCATCAATTTTGTTGTGTTCAGCTATGAGTCGATCGAGAAggg                                                                                                                                   <  2:82684/251‑1 (MQ=255)                                                                                                  gcAGGCTGTGGTCAACCTGGAGGGTAAACCCTCCGTTTGCGGCATACTGATTGAGCTCTGTAGCGAGCGAGTCCGGTGCGATATTAAACTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCAAAGGTGTTGTTTTACGAAAAACGCGTAACGGCGTCATACCTTCCCCATCATCATTTTTGTTGTTTTCAGCTATGAGTCGAACGTGAAGGTTaaaa                                                                                                                              <  2:280/250‑1 (MQ=255)                                                                                                   cAGGCTTGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCAAAGGTGTTGTTTTACGAAAAACGCGTAACGGCGTCATACCTTCCCCATCAGCATTTTTGTTGGGTTCAGCTATGAGTCGAACGAGGAGGGTaaaaa                                                                                                                             <  2:122454/251‑1 (MQ=255)                                                                                                   cAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGGGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGGAGCAGTCTCAGGCGAATGGCGTTAACCAAAGGTGTTGTTTTACGAGATACGCGTAACGGCGTCATACCTTCCCCATCATCATTTTTGTTGTGTTCAGCTATGAGTCGAACGAGAAGGGTaaaa                                                                                                                              <  1:158826/250‑1 (MQ=255)                                                                                                    aggctatcgtaaacagagaGGGAAAATCCGCTGTGTGCGGCATACTGCTTGAGCGCTTTATCGAGCGATCCCGCTGCGATATTAACCTGTGCAGCCAAAGCGGAAAACGCGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGGTAACCAACGGTGTTGTTTTACGGAAAACGCGTACCGGAGTCATACATTCCCCATCCTCATTTTTGTTGTGTTCAGCTATGAGTCGACCGAGGAGGGAaaaa                                                                                                                              >  2:183061/16‑249 (MQ=255)                                                                                                    aGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCAAAGGTGTTGTTTTACGAAAAACGCGTAACGGCGTCATACCTTCCCCATCATCATTTTTGTTGTGTTCAGCTATGAGTCGAACGAGAAGGGTAAAAAg                                                                                                                            <  2:50879/251‑1 (MQ=255)                                                                                                        tGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACATGTGCAGCCAACGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCAAAGGTGTTGTTTTACGAAAAACGCGTAACGGCGTCATACCTTCCCCATCATCATTTTTGTTGTGTTCAGCTATGGGTCGAACGAGAAGGGCAAAAAGGAc                                                                                                                         >  2:35257/1‑250 (MQ=255)                                                                                                           cGTCAACCGGGAGGTGAAATCCGCTTTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAAGGGCGTTAACCAAAGGTGTTGTTTTACGAAAAACGCGTAACGGCGTCATACCTTCCCCATCATCATTTTGGTTGTGTTCAGCTATGAGTCGAACGAGAAGGGTAAAAAGGACAATc                                                                                                                     <  1:236885/251‑1 (MQ=255)                                                                                                           cGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCAAAGGTGTTGTTTTACGAAAAACGCGTAACGGCGTCATACCTTCCCCATCATCATTTTTGTTGTGTTCAGCTATGAGTCGAACGAGAAGGGTAAAAAGGACAATc                                                                                                                     <  1:131591/251‑1 (MQ=255)                                                                                                            gTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCAAAGGTGTTGTTTTACGAAAAACGCGTAACGGCGTCATACCTTCCCCATCATCATTTTTGTTGTGTTCAGCTATGAGTCGAACGAGAAGGGTAAAAAGGACAATCg                                                                                                                    <  2:97646/251‑1 (MQ=255)                                                                                                            gTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCAAAGGTGTTGTTTTACGAAAAACGCGTAACGGCGTCATACCTTCCCCATCATCATTTTTGTTGTGTTCAGCTATGAGTCGAACGAGAAGGGTAAAAAGGACAATCg                                                                                                                    >  1:168668/1‑251 (MQ=255)                                                                                                            gTCAACCGAGAGGGTAAAGCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCAAAGGTGTTGTTTTACGAAAAACGCGTAACGGCGTCATACCTTCCCCATCATCATTGTTGGTGTGTTCAGCTATGAGTCGAACGAGAAGGGTAAAAAGGACAATCg                                                                                                                    <  2:90859/251‑1 (MQ=255)                                                                                                                   gagcggggaaaTCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGCGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCAAAGGTGTTGTTTTACGAAAAAAGCGTAACGGCGTCATACCTTCCCCATCATCATTTTTGTTGTGTTCAGCTATGCGTCGAACGAGCAGGGTAAAAAGGACAATCGAAATa                                                                                                               >  2:47154/9‑249 (MQ=255)                                                                                                                     gagGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCATGCTCAGGCGAATGGCGTTAACCAAAGGTGTTGTTTTACGAAAAACGCGTAACGGCGTCATACCTTCCCCATCATCATTTTTGTTGTGTTCAGCTATGAGTCGAACGAGAAGGGTAAAAAGGACAATCGAAATAAGaa                                                                                                           <  1:163532/251‑1 (MQ=255)                                                                                                                              gccGCTGTGTGCGGCAGACTGAGTGAGCGCTTTATCGAGCGGTCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCAAAGGTGTTGTTTTACGAAAAACGCGTAACGGCGTCATACCTTCCCCAGCATCATTTTTGTTTTGTTCAGCTATGAGTCGAACGAGAAGGGTAAAAAGGACAATCGAAATAAGAATTATTTTcc                                                                                                  <  2:22862/250‑1 (MQ=255)                                                                                                                                      gtgCGGCATACTGATTGAGCGCTTTAGCGAGCGAGCCAGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCAAAGGTGTTGTTTTACGAAAAACGCGTAACGGCGTCATACCTTCCCCATCATCATTTTTGTTGTGTTCAGCTATGAGTCGAACGAGAAGGGTAAAAAGGACAATCGAAATAAGAATTATTTTCCTTACAgt                                                                                           <  2:155715/250‑1 (MQ=255)                                                                                                                                                      gAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCAAAGGTGTTGTTTTACGAAAAACGCGTAACGGCGTCATACCTTCCCCATCATCATTTTTGTTGTGTTCAGCTATGAGTCGAACGAGAAGGGTAAAAAGGACAATCGAAATAAGAATTATTTTCCTTACAGTGGTGAAATGTTTATcc                                                                           <  2:135848/250‑1 (MQ=255)                                                                                                                                                      gAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCGCAGGCGAATGGCGTTAACCAAAGGTGTTGTTTTACGAAAAACGCGTAACGGCGTCATACCTTCCCCATCATCATTTTTGTTGTGTTCAGCTATGAGTCGAACGAGAAGGGTAAAAAGGACAATCGAAATAAGAATTATTTTCCTTACAGTGGTGAAATGTTTATcc                                                                           <  2:237399/250‑1 (MQ=255)                                                                                                                                                       ggcgcTTGATCGAGCGATCCCGGTGCGATACTAACCGGTGCAGCAAAAGCGGAAAACGAGAGGCCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCAAAGGTGTTGTTGTACGAAAAACGCGTAACGGCGTCATACCTGCCCCAACATCATTTGTGTTGTGTGCAGCTATGATGCGAACGAGAAGGGTAAAAAGGACAATCGAAATAAGAATTATTTATCGTACAGTGATGAAATGTTTATCCAg                                                                         <  2:31849/250‑1 (MQ=255)                                                                                                                                                           cTTTATCGAGCGATCCCGAAGCGATATTAACCTGTGCAGCAAAAGCGGACAACGAGAGCCCGGCCATCGGCAGCAGGCTCAGGCGACTGGCGTTAACCAAAGGTGTTGTTTTACGCAACACGCGTAACGGCGTCATACCTTCCCCATCATCATTTTTGTTGTGTTCAGCTATGAGTCGAACGGGAAGGGTACAAAGGCCAATCGAAATAAGGATTATTTTCCTTACAGTGGTGAAATGTTTATCCAGTAcc                                                                     >  2:107414/1‑251 (MQ=255)                                                                                                                                                             ttATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCAAAGGTGTTGTTTTACGAAAAACGCGTAACGGCGTCATACCTTCCCCATCATCATTTTTGTTGTGTTCAGCTATGAGTCGAACGAGAAGGGTAAAAAGGACAATCGAAATAAGAATTAGTTTCCTGACAGTGGTGAAATGTTTATCCAGTAcc                                                                     <  2:112456/249‑1 (MQ=255)                                                                                                                                                              tATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCAAAGGTGTTGTTTTACGAAAAACGCATAACGGCGGCATACCTGCCCCATCATCATTTTTGTTGTGGTCAGCTATGAGTCTAACTAGAAGGGTAAAAAGGACAATCGAAATAAGAATTATGTTCCTGACAGTGGTGAAATGTGTATCCTGTACcgc                                                                   <  2:266486/250‑1 (MQ=255)                                                                                                                                                                             gTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCAAAGGTGTTGTTTTACGAAAAACGCGTAACGGCGTCATACCTTCCCCATCATCATTTTTGTTGTGTTCAGCTATGAGTCGAACGAGAAGGGTAAAAAGGACAATCGAAATAAGAATTATTTTCCTTACAGTGGTGAAATGTTTATCCAGTACCGCGTAATAGACTGAAttt                                                   >  1:266096/1‑251 (MQ=255)                                                                                                                                                                               gCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCAAAGGTGTTGTTTTACGAAAAACGCGTAACGGCGTCATACCTTCCCCATCATCATTTTTGTTGTGTTCAGCTATGAGTCGAACGAGAAGGGTAAAAAGGACAATCGAAATAAGAATTATTTTCCTTACAGTGGTGAAATGTTTATCCAGTACCGCGTAATAGACTGAAtttt                                                  <  1:223535/250‑1 (MQ=255)                                                                                                                                                                                  atatTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCAAAGGTGTTGTTTTACGAAAAACGCGTAACGGCGTCATACCTTCCCCATCATCATTTTTGTTGTGTTCAGCTATGAGTCGAACGAGAAGGGTAAAAAGGACAATCGAAATAAGAATTATTTTCCTTACAGTGGTGAAATGTTTATCCAGTACCGCGTAATAGACTGAATTTTAACg                                              <  1:36619/251‑1 (MQ=255)                                                                                                                                                                                    atTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCAAAGGTGTTGTTTTACGAAAAACGCGTAACGGCGTCATACCTTCCCCATCATCATTTTTGTTGTGTTCAGCTATGAGTCGAACGAGAAGGGTAAAAAGGACAATCGAAATAAGAATTATTTTCCTTACAGTGGTGAAATGTTTATCCAGTACCGCGTAATAGACTGAATTTTAACggg                                            <  1:200877/251‑1 (MQ=255)                                                                                                                                                                                     ttAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCAAAGGTGTTGTTTTACGAAAAACGCGTAACGGCGTCATACCTTCCCCATCATCATTTTTGTTGTGTTCAGCTATGAGTCGAACGAGAAGGGTAAAAAGGACAATCGAAATAAGAATTATTTTCCTTACAGTGGTGAAATGTTTATCCAGTACCGCGTAATAGACTGAATTTTAACggg                                            >  1:136418/1‑250 (MQ=255)                                                                                                                                                                                                  caAAAGCGGAAAACGAGAGACCCGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCAAAGGTGTTGTTTTACGAAAAACGCGTAACGGCGTCATACCTTCCCCATCATCATTTTTGTTGTGTTCAGCTATGAGTCGAACGAGAAGGGTAAAAAGGACAATCGAAATAAGAATTATTTTCCTTACAGTGGTGAAATGTTTATCCAGTACCGCGTAATAGACTGAATTTTAACGGGAAGCGTTTGcgcg                               >  2:176523/1‑250 (MQ=255)                                                                                                                                                                                                             aaCGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCAAAGGTGTTGTTTTACGAAAAACGCGTAACGGCGTCCTACCTTCCCCATCATCATTTTTGTTGTGTTCAGCTATGAGTCGAACGAGAAGGGTAAAAAGGACAATCGAAATAAGAATTATTTTCCTTACAGTGGTGAAATGTTTATCCAGTACCGCGTAATAGACTGAATTTTAAAGGGAAGCGTTTGCGCGATAACGTTCAgg                   >  1:72020/1‑251 (MQ=255)                                                                                                                                                                                                              aCGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCAAAGGTGTTGTTTTACGAAAAACGCGTAACGGCGTCATACCTTCCCCATCATCATTTTTGTTGTGTTCAGCTATGAGTCGAACGAGAAGGGTAAAAAGGACAATCGAAATAAGAATTATTTTCCTTACAGTGGTGAAATGTTTATCCAGTACCGCGTAATAGACTGAATTTTAACGGGAAGCGTTTGCGCGATAACGTTCAGGa                  <  1:8083/251‑1 (MQ=255)                                                                                                                                                                                                               cGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCAAAGGTGTTGTTTTACGAAAAACGCGTAACGGCGTCATACCTTCCCCATCATCATTTTTGTTGTGTTCAGCTATGAGTCGAACGAGAAGGGTAAAAAGGACAATCGAAATAAGAATTATTTTCCTTACAGTGGTGAAATGTTTATCCAGTACCGCGTAATAGACTGAATTTTAACGGGAAGCGTTTGCGCGATAACGTTCAGGAt                 <  1:224069/251‑1 (MQ=255)                                                                                                                                                                                                                  gagaCCGGACAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCCAAGGCGTTGTTTTACGAAAAACGCGTAACGGCGTCATACCTTCCCCATCATCATTTTTGTTGTGTTCAGCTATGAGTCGAACGAGAAGGGTAAAAAGGACAATCGAAATAAGAATTATTTTCCTTACAGTGGAGAAATGTTTATCCAGTACCGCGTAATAGACTGAATTTTAACGGGAAGCGTTTGCGCGATAAAGTTCAGGATCGc              >  2:33279/1‑251 (MQ=255)                                                                                                                                                                                                                   agaCCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCAAAGGTGTTGTTTTACGAAAAACGCGTAACGGCGTCATACCTTCCCCATCATCATTTTTGTTGTGTTCAGCTATGAGTCGAACGAGAAGGGTAAAAAGGACAATCGAAATAAGAATTATTGTCCTTACAGTGGTGAAATGTTTATCCAGTACCGCGTAATAGACTGAATGGTAACGGGAAGCGTTTGCGCGATAACGTTCAGGATCGCa             <  2:33141/251‑1 (MQ=255)                                                                                                                                                                                                                          ccAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCAAAGGTGTTGTTTTACGAAAAACGCGTAACGGCGTCATACCTTCCCCATCATCATTTTTGTTGTGTTCAGCTATGAGTCGAACGAGAAGGGTAAAAAGGACAATCGAAATAAGAATTATTTTCCTTACAGTGGTGAAATGTTTATCCAGTACCGCGTAATAGACTGAATTTTAACGGGAAGCGTTTGCGCGATAACGTTCAGGATCGCATCGGTAt      <  2:59181/251‑1 (MQ=255)                                                                                                                                                                                                                              cGGCAGCAGGCTCAGGCGAATGGCGTTAACCAAAGGTGTTGTTTTACGAAAAACGCGTAACGGCGTCATACCTTCCCCATCATCATTTTTGTTGTGTTCAGCTATGAGTCGAACGAGAAGGGTAAAAAGGACAATCGAAATAAGAATTATTTTCCTTACAGTGGTGAAATGTTTATCCAGTACCGCGTAATAGACTGAATTTTAACGGGAAGCGTTTGCGCGATAACGTTCAGGATCGCATCGGTAttttt  <  1:218622/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                    AGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGTGTTAACCAAAGGTGTTGTTTTACGAAAAACGCGTAACGGCGTCATACCTTCCCCATCATCATTTTTGTTGTGTTCAGCTATGAGTCGAACGAGAAGGGTAAAAAGGACAATCGAAATAAGAATTATTTTCCTTACAGTGGTGAAATGTTTATCCAGTACCGCGTAATAGACTGAATTTTAACGGGAAGCGTTTGCGCGATAACGTTCAGGATCGCATCGGTATTTTT  >  NC_000913/4516388‑4516859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑