| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,516,378 | G→A | N100N (AAC→AAT) | fecA ← | ferric citrate outer membrane transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,516,378 | 0 | G | A | 96.9% | 176.5 / NA | 64 | N100N (AAC→AAT) | fecA | ferric citrate outer membrane transporter |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (30/32); minor base C (2/0); total (32/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTG‑GTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCT > NC_000913/4516137‑4516620 | cAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGGACCTCACGCATGGTG‑GTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTccc < 2:126135/251‑1 (MQ=255) gTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGGTGTCCGGCGCGCTGACGCCAGGGATGCGGTCAAGTACCTCACGCAGGGTG‑GTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGGTCAAATACGTCGGTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCGTTTGGTGCGGGCGCGGGCTCCAGCTTCCAGCTATTATTTCCCAGCg < 2:81875/251‑1 (MQ=255) cATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTG‑GTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCATTCGCCTACCACGTTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGt < 2:182077/250‑1 (MQ=255) gCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTG‑GTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTAc > 1:135848/1‑251 (MQ=255) ccAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTG‑GTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTAcc > 1:7281/1‑251 (MQ=255) cAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTG‑GTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCATCGGTTTTACCt > 1:97646/1‑251 (MQ=255) cAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTG‑GTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCt > 1:50879/1‑251 (MQ=255) ggTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTG‑GTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCCCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGGCCAGCTATTATTTCCCAGCGGTTTTACCTGc > 2:196688/1‑251 (MQ=255) gTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTG‑GTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCCGCGGTTTTACCTGc > 1:237399/1‑250 (MQ=255) gCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTG‑GTTGCGCCGGTTTTGGCGAAATCCTCCCGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCt < 2:59491/249‑1 (MQ=255) gCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTG‑GTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCCGCATGTTCCAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCCGGCTCCAGCGTCCAGCTCTTATTTCCCCGCGGTTTTACCTGCAGGCCGCTGc > 2:218622/1‑251 (MQ=255) ccgctgttTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTTCCTCACGCATG‑TGTGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCCCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGTTCAGGCCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTCTTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCcag < 2:52172/247‑1 (MQ=255) gttgttTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTG‑GTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCcagc > 1:67238/1‑250 (MQ=255) tgttTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTG‑GTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATAAGTCGTTTTCACGCGCATCACCCCCCCAGCCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTACGGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCCGCAgt > 1:45812/1‑251 (MQ=255) tttCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTG‑GTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAgttgt > 1:196731/1‑251 (MQ=255) ttCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTG‑GTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCCGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAgttgtt > 1:175091/1‑251 (MQ=255) tCCGGCGCGCTGACGCCAGGGGTGAGATTAAGTACCTCCCGAATGGTG‑GTTGCGCCGGCTTTGGCGAACTCCTCCCGGCGGCTCCCGTCACGCGCGACAGGATGTTCAAATACGTCGTTTTCACGCGCATCACCCCACCAGCCGCCCACCACGGTCAGGGCATCTTTTTTTGGTGCAGGCGCCGGCACCCGCGTCCAGCTATTATTTCCCAGTGGTTTTACCTGCAGTCCGCTGCCGTCCCGCAGttgttg > 2:249917/1‑251 (MQ=255) ggcgcTGACGATAGGGATGCGGTTAAGTCCCTCACGCATGGTG‑GTTGCGCCGGTGTTGGCGAAATCCTCACGGCGGATCTCGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTGCACGCGCATCACCCAGCCAGTCGCCGACCTCGGTCAGGGCATCGTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGc < 1:267874/250‑1 (MQ=255) gCCAGGGATGCGGTTAAGTACCTCACGCATGGTG‑GTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCgc > 2:121257/1‑250 (MQ=255) aGGGATGAGGTTAAGTACCTCACGCATGGTG‑GTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTTACGCGCGCCAGCCTGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTCTTATTTCCCAGCGGTTTTTCCTGCAGTCCGATGCCGTCCAGCTGTTGTTGCAGGCCGCTCTCGAcgc > 2:36619/1‑250 (MQ=255) gggATGCGGTTAAGTACCTCACGCATGGTG‑GTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGAcgtc > 1:112456/1‑251 (MQ=255) gggATGCGGTTAAGTACCTCACGCATGGTG‑GTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGGGCGGGCGCGGGCTCCAGCGTCCAGCTATTAGTTCCCAGCGGTTTTACCTGCAGTCCGCTTCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGAcgtc < 2:230315/251‑1 (MQ=255) gATGCGGTTAAGTACCTCACGCATGGTG‑GTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGCTCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCCTCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCACCCGTTGTTGCAGGCCGCTATCGAcgtcgc > 2:224069/1‑250 (MQ=255) gCGGTTAAGTCCCTCACGCATGGTG‑GTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAAt < 2:119413/251‑1 (MQ=255) gCGGTTAAGTACCTCACGCATGGTG‑GTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCGCGTCACGCGCGCCAGCATGTTCAAATACGGCGGTTTCACGCGCATCACCCAGCCAGGCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCTGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCTGTTTTACCTGCAGTCCTCTGCCGTCCATCAGGTTTTGCAGTCCGCTCTCGACGTCGTAAt < 2:269202/251‑1 (MQ=255) aaggcccTCACGCATGGTG‑GTCGCGCCGGTTTTGGCGAAATCCGCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATTCGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGt < 2:150218/246‑1 (MQ=255) aCCTCACGCTGGGTG‑GTTGGGCCGGGTGTGGGGAGATGCGTACGGCGGATCTCGTCACGCGCGCCAGCATGGTAAAATGGGTCGGTTTCCCGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCGTCTTTTGGTGCGGGGGCGGTCTCCAGCGTCCAGCTATTATTTCCCAGCGGGTTTACCTGCAGTCCGCTGCCGTCGAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAg < 2:19317/251‑1 (MQ=255) ccGCATGGTG‑GTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGt < 2:225998/250‑1 (MQ=255) gCATGGTG‑GTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTAGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCAAGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGACGTGCAGGCCGTTg > 1:155715/1‑251 (MQ=255) cATGGTG‑GTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCTCCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGc < 1:128303/251‑1 (MQ=255) tGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTg < 1:26555/250‑1 (MQ=255) cgcACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCGCTGCTTGCCGCGCGTCAGGCTGGCGTCaa < 2:228038/249‑1 (MQ=255) cgcggcggATCACGTCACGCGCGCAAGCATGTGCAAATACGTCGTTTTCACGCGCCTCCCCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCGGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCGCTCGACGTCGGAATCGCCGTGCAGGCCGTTGCGCTGCTTGCCGCGCGTCAGGCTGGCGTCaa < 2:175091/247‑1 (MQ=255) cACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGGTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCCGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAAcc > 2:225392/1‑251 (MQ=255) cacgtcacgCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTa > 2:163532/1‑250 (MQ=255) cgtcacgCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCCGGCTGGCGTCAACCGCGAGGGTAAAt > 1:122454/1‑251 (MQ=255) gtcacgCGCGCCAGCATGTTCAAATGCGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCTACCACGGTCAGGGCATCTTCTTTTTGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCTGTTGTTGCGGGCCGCTCTCGACTTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATc < 1:127192/251‑1 (MQ=255) cgcCAGCATGTTCAAATACGTCGTTTTCACGAGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCCGAGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCCGGCTGGCGTCACCCGCGAGGGTAAATCCGCtgtgt > 2:239175/1‑251 (MQ=255) tGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTAGCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTACTCGCCGCGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCAt > 1:107237/1‑250 (MQ=255) ttCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGAGCGGCATACt > 1:59181/1‑251 (MQ=255) ttCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACAACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCCGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCAGCTCTAGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGCCTGGCGGCACACGAGAGGGTAAATCTGTTGTGTGCGGCATACt > 1:156807/1‑251 (MQ=255) tCAAATACGTCGTTTTCCCGCGCCTCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGGGCGGGCGCGGGCTTCAGCGTCCAGCTAGTATTTCCCAGCGGTGTTACCTGCAGTCCGCGGCCGGCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAAGCGCCGTGCAGGCCGTTGCTCTGCTTTCCGCGCGTCAGGCTGGCGTTTACCGAGAGGGTAAATCCGCTGTTTGCGGCATACt < 2:175828/250‑1 (MQ=255) aaaTACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGAt < 1:225926/251‑1 (MQ=255) ttCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTAt > 2:200877/1‑251 (MQ=255) tCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATc > 1:33141/1‑251 (MQ=255) cccgcgCAGCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCTTTGCTCTGCTTGCCGCGCGGCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCGGTGTGCGGCAGACTGATTGAGCGCTTTATc < 2:29168/248‑1 (MQ=255) cgaccATCACCCAGCCAGGCGCCTACCACGGTCAGGGCATCGTCTGTTGGTGCGGGCGCGGGCTCCAGGGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTGGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAg < 1:12499/247‑1 (MQ=255) ccgcATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGc < 2:8485/250‑1 (MQ=255) aTCACCCAGCCAGTCGCAGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTTCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATTCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATc < 1:84358/251‑1 (MQ=255) ccagccagTCGCCGACCACGGTCAGGGCATCTTATTTTGGTGCGGGAGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTCCCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGCGCGATCCCGGt > 1:255139/1‑251 (MQ=255) agccagTCGCCGACCACGGGCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGGCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGc < 1:248898/251‑1 (MQ=255) gccagTCGCCGTCCACGGTCAGGGCATCTTCTTTTGGTGCGGGAGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCACGGTGCg < 1:148858/251‑1 (MQ=255) cGCCGACCACGGTCATGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCGGGCGTCATCCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTa < 1:238624/251‑1 (MQ=255) cACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCGTTATCGAGCGATCCCGGTGCGATATTAACCtgtg < 1:83798/251‑1 (MQ=255) gTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGGAAATCCTCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGGGCGATATTAACCTGTgcagc > 2:221259/1‑251 (MQ=255) cttTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCTCGCGATCCCGGTGAGAGATTAACCTGTGCAGCAAAAGCGGaaaa > 2:78578/1‑250 (MQ=255) tttGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCAGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGCGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACg > 2:46894/1‑250 (MQ=255) gcgggcgcgggcCCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGAcc < 1:147226/251‑1 (MQ=255) gggcgcgggcTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGCCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCgg < 2:173825/251‑1 (MQ=255) cgcgggcTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCa < 2:196731/251‑1 (MQ=255) cgcgggaTCCAGAGACCGGCTATTCTTTCCCAGCGGTTTTCCCTGCCGTCAGCAGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCACCGTGCCGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACAGCGAGGGTAAATCCGCTGTGGTCGGAGTACTGATTGCGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGGGAAGCAAAAGCGGAAAAAGAGAGACCGGccc > 2:236885/1‑250 (MQ=255) ggcTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCgg < 1:95311/251‑1 (MQ=255) tCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGgc > 1:5289/1‑249 (MQ=255) gTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCt < 1:189398/251‑1 (MQ=255) | CAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTG‑GTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCT > NC_000913/4516137‑4516620 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |