Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,514,287	G→C	L8V (CTT→GTT)	fecB ←	ferric citrate ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,514,287	0	G	C	100.0%	94.3 / NA	30	L8V (CTT→GTT)	fecB	ferric citrate ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (12/18);  total (12/18)

GCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGATGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCTGGTCGAAGATGTTTTTCACACCGAATGCCAGGTTCAGATCTGCCATCTGCGGGCCAAAGTCATACGCCACGCGTGCGC  >  NC_000913/4514050‑4514525                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                               gcgTTCCGACGTACTGCCACGGTGTCAGGTGCGCACGCTGTGCGGGCAGGATGCGTTTTTCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAAGCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCTTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCATGCCTGCAAAAACAAAACAGATAAAt                                                                                                                                                                                                                                   <  2:89684/251‑1 (MQ=255)                gcctcGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTAGCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGTCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGAGCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATcaca                                                                                                                                                                                                                    <  1:136856/247‑1 (MQ=255)                 ccACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACAt                                                                                                                                                                                                                   <  1:121226/251‑1 (MQ=255)                    cGGTTTCAGGTGCGCACGCACTGCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTGCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTcc                                                                                                                                                                                                                <  1:94107/251‑1 (MQ=255)                          cAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTTGCGTCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTa                                                                                                                                                                                                           <  1:181415/250‑1 (MQ=255)                          cAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGTATAAATGCCAACATAATCACATTCCAGCTaa                                                                                                                                                                                                          <  2:177788/251‑1 (MQ=255)                                                aGGATGCGGTTTGCATCGGTATCGTCGGCAATACCGATCGGGCTGACGGCCACGGCGGTCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAAGCACATTCCATCTAAAAGCCCGGCAAGCCGGTCGTTa                                                                                                                                                                                    <  2:191241/251‑1 (MQ=255)                                                aGGATGAGTTTTGCATCGTTATCGTCGGCAATACTGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAGCAAAACGGATAAATGCCAACATAAACACATTCCAGCCAAAAGCCCGGCCAGCCGGGCGtt                                                                                                                                                                                     >  2:5652/1‑250 (MQ=255)                                                        ttttGCATCGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATc                                                                                                                                                                             >  2:232000/1‑250 (MQ=255)                                                              aTCGTTCTCGTCGGCACTACAGCTCGGGCTGCCGTCCACGGCGGCCAGCGCCTCGGAGCACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAACCGAGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCGCCAGCAACAGGCCTGCAAACACAACACGGATAAATGCCAACCTAATAACATTCCAGCTCAAAACCAGGCACGCCGGGCGATAACACATAAGCACtt                                                                                                                                                                      >  2:156751/1‑251 (MQ=255)                                                                cGTTATCGTCGGCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACAGTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCa                                                                                                                                                                    <  1:160911/251‑1 (MQ=255)                                                                          ggCAATACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGATGTGGCGTTTTTTCGAGTGTACACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGGTAACACATCAGAACTTCAACggaccctg                                                                                                                                                          >  1:120146/1‑244 (MQ=255)                                                                              aTACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCata                                                                                                                                                      <  1:232000/251‑1 (MQ=255)                                                                               tACCGATCGGGCTGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCatat                                                                                                                                                     >  1:137727/1‑251 (MQ=255)                                                                                           tGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGAGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGcgcg                                                                                                                                         >  1:261261/1‑251 (MQ=255)                                                                                             aCGTCAACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACCTCAGAACTTCAACGACCCCTGCCTATACAGCGTGCGCGGc                                                                                                                                       >  2:245488/1‑251 (MQ=255)                                                                                                   aCGGCGGCCAGCGCAGCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCt                                                                                                                                 <  1:132822/251‑1 (MQ=255)                                                                                                            aGCGCATAGGCGAACGAGAGCTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGCGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATCAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATg                                                                                                                        >  2:168956/1‑251 (MQ=255)                                                                                                                        aaCGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGACCTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTttgttgttg                                                                                                            >  1:134781/1‑251 (MQ=255)                                                                                                                        aaCGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATAAAGCGTGCGCGGCTGGCCTGCATAGATGCCTttgttgttg                                                                                                            >  1:222600/1‑251 (MQ=255)                                                                                                                                                              ttCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCTGGTCGAAGa                                                                       <  1:42424/250‑1 (MQ=255)                                                                                                                                                                   gtgtAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCTGGTCGAAGATGtttt                                                                 <  1:71827/251‑1 (MQ=255)                                                                                                                                                                      gaaaCGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCTGGTCGAAGATGTTTTTca                                                              <  2:121442/250‑1 (MQ=255)                                                                                                                                                                       aaaCGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCTGGTCGAAGATGTTTTTcac                                                             >  1:244806/1‑251 (MQ=255)                                                                                                                                                                                      gTCCTGACCCGGGGCGGCAAAGGCGTGGCTGATCCCCAGCAGCATGCCTGCAAAAACAAAACGTAGCAATGCCAACATAAGCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACAACATAACTTCAACGACCCCTGCATATACTGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATAAGTGCGGATGAAGTAGTCCTGGTCGAATATGTATTTCACACCGAATGCCAGGtt                                              <  2:50714/251‑1 (MQ=255)                                                                                                                                                                                         cggAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCTTGCGCGTCTGGCCTGCATAGATGCCGTTGTTGTTGTCGTCATAAGATCGTATGAAGTAGGCCTGGTCGAAGATGGTTTTCACACCGAATGCCAGTTTCAg                                           <  2:252576/249‑1 (MQ=255)                                                                                                                                                                                                          aGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCTGGTCGAAGATGGTTTTCACACCGAATGCCAGGTTCAGATCTGCCATCTGCGGGc                          <  1:236099/251‑1 (MQ=255)                                                                                                                                                                                                                     aTCACCAGCAGCAGTCCTGCAAAAACAAAACGGATAAATGCCAAAATAATCACATTCCAGCTAAAAGCCCGGAAATCCGGGCGTTAACACATCAGAACGGCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCGTTTGTTGTTGTCGTCATAAGAGCTGATGAAGTAGTCCTGGTCGAAGATGTTTTTCACCCCGAATGCAAGGTTCAGATCAGCCATCTGCTGCCCAAAGTAATAc               <  2:138969/251‑1 (MQ=255)                                                                                                                                                                                                                             cagcagGCCTGCAAAAACAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCTGGTCGAAGATGTTTTTCACACCGAATGCCAGGTTCAGATCTGCCATCTGCGGGCCAAAGTCATACGCCAcgcg       <  1:188890/251‑1 (MQ=255)                                                                                                                                                                                                                                  ggCCTGCAAAAACAAAAAGGATAAATGCCAACATAATCACATTCCCGCTAAAACCCAGGCAAGCCGGGCGTTACCCCATCAGAAACTCACCCACCCCTGCAAATACATCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCCTAAGAGCGGATGAAGTAGTCATGGTAGAAGATGTTTTTCACCCCGCATGACAGGTTCAGATCTGCCATCTGCGGGCCAAAGTCATACGCAACCCGTgcgc  >  1:23527/1‑251 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                               GCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGATGACGTCCACGGCGGCCAGCGCATCGGCGAACGAGAGTTCCAGCACCACAATCCGTTGTGGCGTTTTTTCGAGTGTAAACGTGCCGTGTTCGTCCTGAACCGTGGCGGCAAAGGCGTGGCTGATCACCAGCAGCAGGCCTGCAAAAAGAAAACGGATAAATGCCAACATAATCACATTCCAGCTAAAAGCCCGGCAAGCCGGGCGTTAACACATCAGAACTTCAACGACCCCTGCATATACAGCGTGCGCGGCTGGCCTGCATAGATGCCTTTGTTGTTGTCGTCATAAGAGCGGATGAAGTAGTCCTGGTCGAAGATGTTTTTCACACCGAATGCCAGGTTCAGATCTGCCATCTGCGGGCCAAAGTCATACGCCACGCGTGCGC  >  NC_000913/4514050‑4514525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑