Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,513,841	A→G	R156R (CGT→CGC)	fecB ←	ferric citrate ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,513,841	0	A	G	100.0%	134.1 / NA	43	R156R (CGT→CGC)	fecB	ferric citrate ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/21);  total (22/21)

GATAGTGGGCAACCAGCAGCCAGGCAGGATTGACCGCCAGCAGTTGCTCCAGGCCGATGGACGGCATGGACGCACCCGCCATCGCAGCGGGAACGTTCAGCCCCAGAGAGGCCAGCACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAAGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTAAGCAGCAGTACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTG  >  NC_000913/4513597‑4514080                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                gATAGTGGGCAACCAGCAGCCAGGCAGGATTGTCCGCCAGCAGTTGCTCCAGGCCGATGGACGGCATGGACGCACCCGCCATCGCAGCGGGAACGTTCAGCCCCAGAGCGGCCCGCACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAAGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTTTTCCAGGCGTGcc                                                                                                                                                                                                                                           >  2:86834/1‑251 (MQ=255)  aTAGGGGGCAACCAGTAGCAAGGCAGGATTGACCGCCAGCAGTTGCTCCAGGCCGATGGACGGCATGGACGCACCCGCCATCGCAGCGGGAACGTTCAGCCCCAGAGAGGCCAGCACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAAGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGGCGTGCCt                                                                                                                                                                                                                                          <  1:79491/251‑1 (MQ=255)    ggggggCAACCAGCGCCCAGGCAGGTTGGACCGCCAGCAGTTGCTCGAGGCCGATGGACGGCATGGACGCACCCGCCATCGCAGCGGGAACGTTCAGCCCCAGAGAGGCCAGCACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAAGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTGGTTCCAGGCGTGCCTGc                                                                                                                                                                                                                                        <  2:120838/248‑1 (MQ=255)               agcagcCAGGCAGGATTGACCGCCAGCAGTTGCTCCAGGCCGATGGACGGCATGGACGCACCCGCCATCGCAGCGGGAACGTTCAGCCCCAGAGAGGCCAGCACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAAGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGGCGGGCCTGCATCTCTCGCtt                                                                                                                                                                                                                             >  2:73928/1‑251 (MQ=255)                  agcCAGGCAGGATTGACCGCCAGCAGTTGCTCCAGGCCGATGGACGGCATGGACGCACCCGCCATCGCAGCGGGAACGTTCAGCCCCAGAGAGGACAGCACGCTGCCGGTCCCGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAAGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGGCGTGCCTGCATCTCTCGCttttt                                                                                                                                                                                                                          >  1:50714/1‑251 (MQ=255)                             atgtcccGCCAGTAGTTGCCCCAGGCGGAGGGACGGCAGGGCCCCACCCGCCACCGCAGCGGGAACGTTCAGCCCCAGAGAGGCCAGCACGCTGCCGGGCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAAGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGGCGTGCCTGCATCTCTCGCTTTTTACCCACCAttt                                                                                                                                                                                                               <  2:44263/246‑1 (MQ=255)                                   cGCCAGCAGTTGCTCCAGGCCGATGGACGGCATGGACGCACCCGCCATCGCAGCGGGAACGTTCAGCCCCAGAGAGGCCAGCACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAAGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGGCGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCga                                                                                                                                                                                                         <  1:28949/251‑1 (MQ=255)                                      cagcagTTGCTCCAGGCCGATGGACGGCATGGACGCACCCGCCATCGCAGCGGGAACGTTCAGCCCCAGAGAGGCCAGCACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAAGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGGCGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCgatg                                                                                                                                                                                                       >  1:267673/1‑250 (MQ=255)                                             tGCTCCAGGCCGATGGACGGCATGGACGCACCCGCCATCGCAGCGGGAACGTTCAGCCCCAGAGAGGCCAGCACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAAGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGGCGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATAGCCg                                                                                                                                                                                                <  1:209678/250‑1 (MQ=255)                                                   aGGCCGATGGACGGCATGGACGCACCCGCCAGCGCAGCGGGAACGTTCAGCCCCAGAGAGGCCAGCACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAAGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGGCGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATAGCCGCAGATTg                                                                                                                                                                                         <  1:117707/251‑1 (MQ=255)                                                                      aCGCACCCGCCATCGCAGCGGGAACGTTCAGCCCCAGAGAGGCCAGCACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAAGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGGCGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGtt                                                                                                                                                                      >  1:29716/1‑251 (MQ=255)                                                                          ccccGCCATCGCAGCGGGAACGTTCAGCCCCAGAGAGGCCAGCACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAAGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGGCGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGt                                                                                                                                                                  <  1:241261/250‑1 (MQ=255)                                                                                 cccGCAGCGGGAACGGTCAGCCCCAGAGAGGCCAGCACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGACGTGCCAAAGGCCACGCGTGTCCCTGTGGGAAGCTGGCTGGCCCACTGCTCCATCCTCTCATTATGTTGTTCAAGGCGTGCCTGCATCTCTCGCTTTTTACCAACCATTTCGCCGAGGATAGCCGCAGATTGTAAATTTTCAGCGTAGGTTTCGTTGCGGGa                                                                                                                                                           <  2:267673/249‑1 (MQ=255)                                                                                   cGCAGCGGGAACGTTCAGCCCCAGAGAGGCCAGCACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAAGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGGCGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACt                                                                                                                                                         >  2:55140/1‑251 (MQ=255)                                                                                               gTTCAGCCCCAGAGAGGCCAGCCCGCTGCCGGTCCAGGTCTCCTGAGTCTGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAAGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGGCGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTAAGCAGCAGt                                                                                                                                              >  2:13324/1‑250 (MQ=255)                                                                                               gTTCAGCCCCAGAGAGGCCAGCACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAAGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGGCGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTAAGCAGCAGTa                                                                                                                                             <  1:160604/251‑1 (MQ=255)                                                                                                  cAGCCCCAGAGAGGCCAGCACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAAGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCATGCGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTAAGCAGCAGTACCg                                                                                                                                          <  1:139405/251‑1 (MQ=255)                                                                                                  cAGCCCCAGAGAGGCCAGCACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAAGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGGCGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTAAGCAGCAGTACCg                                                                                                                                          <  1:13324/251‑1 (MQ=255)                                                                                                  cAGCCCCAGAGAGGCCAGCACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTGCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAAGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGGCGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGGAGGTTTCGTTGCGGGACTTAAGCAGCAGTAcc                                                                                                                                           <  2:91427/250‑1 (MQ=255)                                                                                                         agagagGCCAGCACGCTGCCGGTCCCGGTCTCCTGCGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAAGCTGGCTGGCCCACTGAGCCATCCTCTCTTTATGTTGTTCCAGGCGTGACTGCATCTCTCGCTTTTTACCCCCCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTCGGTTTCGTTGCGGGACTTAAGCAGCAGTACCGGcgcgtt                                                                                                                                   >  2:94107/1‑249 (MQ=255)                                                                                                          gagagGCCAGCACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAAGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGGCGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTAAGCAGCAGTACCGGCGCGAtt                                                                                                                                  >  1:2983/1‑251 (MQ=255)                                                                                                                          gCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAAGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGGCGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTAAGCAGCAGTACCGGCGCGATTTGCTGCAAGGCGATGt                                                                                                                  >  1:191241/1‑251 (MQ=255)                                                                                                                                        ccTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAAGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGGCGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTAAGCAGCAGTACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCAt                                                                                                      <  1:909/249‑1 (MQ=255)                                                                                                                                                tGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAAGCTGGCTGGCACACTGCGCCATCCTCTCTTTATGTTGTTCCAGGAGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATAGACGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTAAGCAGCAGTACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGActgct                                                                                            >  2:34271/1‑251 (MQ=255)                                                                                                                                                     gTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGACGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGGCGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATAGCCGCAGCTTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTAAGCAGCAGTACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCgg                                                                                       >  1:252576/1‑251 (MQ=255)                                                                                                                                                     gTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAAGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGGCGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGAGAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTAAGCAGCAGTACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCgg                                                                                       <  2:128377/251‑1 (MQ=255)                                                                                                                                                      ttGAATTGCTGTTCGCGTGATGTGCAAAAGGCCACGCGTGTCCCTTTGGGAAGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGGCGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTAAGCAGCAGTACCGGCGCGATTTGATGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGc                                                                                      >  2:259048/1‑251 (MQ=255)                                                                                                                                                         aaTTGCTGTTCGCGTGATGTGCCAAAGGCCCCGCGTGTCCCTGTGGGAAGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGGCGTGCCTGCATCGCTCGCTTTTTACCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTAAGCAGCAGTACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAAt                                                                                   <  2:522/251‑1 (MQ=255)                                                                                                                                                                gTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAAGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGGCGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTAAGCAGCAGTACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAg                                                                             <  1:207902/250‑1 (MQ=255)                                                                                                                                                                gTTCGAGTGATGTGCCAACGGCCACGCGTGTCCCTTTGGGAAGCTGGCTGACCCACTGCGCCATCATCTCTTTATGTTGTTCCAGGCGTGCCTGCATCCCTCGCTTTTTACCCACCATTTCGCCGATGATAGCCGCAGCTTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTAAGCAGCAGTACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAgg                                                                            >  2:181415/1‑251 (MQ=255)                                                                                                                                                                        gATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAAGCTGGCGGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGGCGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTAAGCAGCAGTACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAGGTCTGGttt                                                                    <  1:76170/251‑1 (MQ=255)                                                                                                                                                                            tgCCAAAGGCCACGCGTGTCCCTTTGGGACGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGGCGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTAAGCAGCAGTACCGGCGCGCTTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTc                                                                   >  2:218869/1‑248 (MQ=255)                                                                                                                                                                                           gtgtCCCTTTGGGAAGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGGCGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTAAGCAGCAGTACCGGCGCGATTTGCTGCAAGGCGATGTAAAACCCCGCATGGCGACTGCTGTCGGCAATAATCAGGTCTGGTTTCAGAGCGGCAATGGCTTcc                                                 >  2:132822/1‑251 (MQ=255)                                                                                                                                                                                                     gggAAGCTGGCTGTCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGGCGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTAAGCAGCAGTACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCCATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCt                                       >  2:109868/1‑251 (MQ=255)                                                                                                                                                                                                        aaGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGGCGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTAAGCAGCAGTACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCGCGGCTgcg                                    <  1:101105/251‑1 (MQ=255)                                                                                                                                                                                                         aGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGGCGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTAAGCAGCAGTACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTgcgc                                   >  2:205628/1‑251 (MQ=255)                                                                                                                                                                                                           ctggctggCCCACTGCGCCATCATCTCTTTATGTTGTTCCAGGCGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTAAGCAGCAGTACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTgcgcgc                                 >  2:236099/1‑251 (MQ=255)                                                                                                                                                                                                             ggctggcCCACTGCGACATCCTCTCTTTATGTTGTTCCAGGCGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGCTAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTAAGCAGCCGTACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCGGCACTGGCTTCCAGGCTCGGCTgcgcgcgc                               >  2:160911/1‑251 (MQ=255)                                                                                                                                                                                                                  gcCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGGCGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTAAGCAGCAGTACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAGGTCGGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTcc                          <  1:37692/251‑1 (MQ=255)                                                                                                                                                                                                                      aCTGCGCCATCCTCTCTTTATGTTGTTCCAGGCGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTAAGCAGCAGTACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACg                      >  2:194778/1‑251 (MQ=255)                                                                                                                                                                                                                                ccTCTCTTTATGTTGTTCCAGGCGTGCCGGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTAAGCAGCAGTACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCAc             <  2:187975/250‑1 (MQ=255)                                                                                                                                                                                                                                   ctctTTATGTTGTTCCAGGCGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTAAGCAGCAGTACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACgg           <  1:55140/249‑1 (MQ=255)                                                                                                                                                                                                                                          ggttgttCCAGGCGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTAAGCAGCAGTACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTg  >  2:71827/2‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                GATAGTGGGCAACCAGCAGCCAGGCAGGATTGACCGCCAGCAGTTGCTCCAGGCCGATGGACGGCATGGACGCACCCGCCATCGCAGCGGGAACGTTCAGCCCCAGAGAGGCCAGCACGCTGCCGGTCCAGGTCTCCTGAGTATGCAGGTTGAATTGCTGTTCGCGTGATGTGCCAAAGGCCACGCGTGTCCCTTTGGGAAGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTAAGCAGCAGTACCGGCGCGATTTGCTGCAAGGCGATGTAAACCCCCGCATGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTG  >  NC_000913/4513597‑4514080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑