| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,507,424 | G→C | pseudogene (228/255 nt) | insO → | IS911B regulator fragment |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,507,424 | 0 | G | C | 93.5% | 83.0 / ‑4.8 | 31 | pseudogene (228/255 nt) | insO | IS911B regulator fragment |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base C (14/15); minor base A (0/2); total (14/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
CCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAGATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAACCAGATCTAGTTCATGTTGAGTATATTGGGCAAGACATGTCTTTTTAGGAAAGTACTGCCGAATTAGCCCATTTGTGTTCTCATTTGTTCCCCGCTGCCAAGGACTCTGAGGA > NC_000913/4507194‑4507670 | ccAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGaa < 1:84181/249‑1 (MQ=255) aaTGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAatat > 2:87414/1‑251 (MQ=255) aaaTTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTAc > 2:80774/1‑251 (MQ=255) agagTTTAAACGCGACTACGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCCGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATATGTAAGCTGAGGAAAAAGCTAAAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgat < 2:89523‑M2/251‑15 (MQ=255) aCGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGAATTGACATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactc < 1:171125‑M2/251‑24 (MQ=255) cgcgAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactcc < 1:208541‑M2/251‑25 (MQ=255) aCTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcg > 2:129864‑M2/1‑213 (MQ=255) tgACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacaggtagcgataatcgg > 2:1896‑M2/1‑204 (MQ=255) tgACAAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACACTGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACAACAGAACAAATCGAAATACGGAAGCTGAGGAAAAAGCTACAACGAATTGAAATGGAGAATGCAATATTAACAAGGCTaccgcgctattgatgacagactccctgaacagttatcgataatcggg < 2:105581‑M2/251‑48 (MQ=255) aCCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGACATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcggga < 2:260631‑M2/250‑49 (MQ=255) aCCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGACTGAAATATTAAAAAGGCTaccgcgatcttgatgtcagactccctgaacagttctcgataatcggga < 2:7244‑M2/250‑49 (MQ=255) cGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTTCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgca > 2:18001‑M2/1‑190 (MQ=255) cAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGAAAGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgaattatcc < 1:135522‑M2/251‑68 (MQ=255) aaGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggttcatcgcagcagc > 1:80774‑M2/1‑141 (MQ=255) ggTCAAACAACTGCGTGATGAGAGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGCAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactcactgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggatcatcgcagcagctac < 1:45779‑M2/248‑114 (MQ=255) aacTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAAACGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggttcatcgcagcagctacagatactggaa > 2:208541‑M2/1‑127 (MQ=255) atgaGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggttcatcgcagcagctacagatactggaaaaaccgtcc > 1:260631‑M2/1‑118 (MQ=255) gTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGACATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggttcatcgcagcagctacagatactggaaaaaccgtcctgaaa < 1:113516‑M2/250‑139 (MQ=255) gTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggttcatcgcagcagctacagatactggaaaaaccgtcctgaaa < 1:41181‑M2/250‑139 (MQ=255) gggCAAAACACCAAAAGCCTCTCCGATAAAACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGATACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgatactcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggttcatcgcagaagctacagatactggaaaaaccgtcctgaagaacc < 2:109730‑M2/250‑143 (MQ=255) tctcCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGCAAAAGCTACAGCGCATTGAAGTAGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactcactgaacagttctcgataatcgggcaactcagggcgcattatcctgtggtaacactctgccgtgtgatcggagtgcatcgcagcagctacagatactggaaaagccgtcctgaaaagccagacggcagacgggctgtat < 1:64543‑M2/251‑163 (MQ=255) gATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAACGCTACCACGCATTGAAATGGAGAATGACATATTAACAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggttcatcgcagcagctacagatactggaaaaaccgtcctgaaaaaccagacggcagacgggctgtattacgc < 1:239261‑M2/251‑168 (MQ=255) aGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggttcatcgcagcagctacagatactggaaaaaccgtcctgaaaaaccagacggcagacgggctgtattacgcagtcaggta > 1:87414‑M2/1‑75 (MQ=255) aGAACAAATCGAAATACGGAAGCAGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggttcatcgcagcagctacagatactgtaaaaaccgccctgaaaaaccagacggcagacgggctgtattacgcagtcaggta > 2:122177‑M2/1‑75 (MQ=255) gAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggttcatcgcagcagct < 1:46444‑M2/185‑112 (MQ=255) gAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggttcagcgcagcagct > 2:46444‑M2/1‑74 (MQ=255) aaTACGTAAGCGGAGGAAAAAGCGACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgctctcttgatgtcagactccctgaacagttctctataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggttcatcgcagcagctacagatacttgaaaaaccgtcctgaaaagccatacggcagacgggccgtagtacgcagtctgttacttgagctacat > 2:171125‑M2/1‑63 (MQ=255) aaaGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgttggcacactctgccatgtgttcggggttcatcgcagcagctacagatgctggaaaaaccgtcctgaaaaaccagacggcagacgggctgtattacgcagtcaggtacttgagctacatggcatcagccacggttcg > 1:89523‑M2/1‑45 (MQ=255) aaaGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggttcatcgcagcagctacagatactggaaaaaccgtcctgaaaaaccagacggcagacgggctgtattacgcagtcaggtacttgagctacatggcatcagccacggttcg > 2:2174‑M2/1‑45 (MQ=255) aaaGCTACAACGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggttcatcgcagcagctacagatactggaaaaaccgtcctgaaaaaccagacggcagacgggctgtattacgcagtcaggtacttgagctacatggcatcagccacggttcg < 1:2174‑M2/251‑207 (MQ=255) aCGCATTGAAATGGAGAATGAAATATTAAAAAGGCTaccgcgctcttgatgtcagactccctgaacagttctcgataatcgggaaactcagagcgcattatcctgtggtcacactctgccatgtgttcggggttcatcgcagcagctacagatactggaaaaaccgtcctgaaaaaccagacggcagacgggctgtattacgcagtcaggtacttgagctacatggcatcagccacggttcggccggagc < 1:59693‑M2/250‑215 (MQ=255) | CCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAGATTCAATCTGTCAATGCAACACCCCTTTCAATTATCTCTTTCGGTGTTTTGAACTTCAGTGTCTTTCTCGGTCTGTTGTTTAGCTGAGCAGCAACCAGATCTAGTTCATGTTGAGTATATTGGGCAAGACATGTCTTTTTAGGAAAGTACTGCCGAATTAGCCCATTTGTGTTCTCATTTGTTCCCCGCTGCCAAGGACTCTGAGGA > NC_000913/4507194‑4507670 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |