Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	5,099	T→C	intergenic (+79/‑135)	thrC → / → yaaX	threonine synthase/DUF2502 domain‑containing protein YaaX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	5,099	0	T	C	100.0%	61.3 / NA	20	intergenic (+79/‑135)	thrC/yaaX	threonine synthase/DUF2502 domain‑containing protein YaaX
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/13);  total (7/13)

GCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCC  >  NC_000913/4864‑5321                                                                                                                                                                                                                                            |                                                                                                                                                                                                                               gcATCCGGCGAAATTTAAAGAGGGCGTGGAAGCGATTCTCGGTGAAACGTGGGATCTGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGgag                                                                                                                                                                                                                <  1:230053/251‑1 (MQ=255)  cATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGGTGGATCTGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGgaga                                                                                                                                                                                                               <  1:59013/251‑1 (MQ=255)             aTTTAAAGAGATCTGGGAAGCGATTCTCGGTGAAACGTTGGAGCTGCCAAAAGAGCGGGCAGAACGTGCTGATTTCCCCTTGCTTTCCCAAAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATa                                                                                                                                                                                                    <  1:8182/251‑1 (MQ=255)                       ggCGTGGAAGCGATTACCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCTTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATACTCAATAAATGCGGTaa                                                                                                                                                                                          <  1:214634/250‑1 (MQ=255)                                   aTTCTCGGTGAAAGGTTGGATCGGCCAAAAGAGCGGGCAGAACGTGCTGATTTACCCTTGCTTTCAAAAAATCGCCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAg                                                                                                                                                                              <  2:249985/251‑1 (MQ=255)                                      ctcGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGAt                                                                                                                                                                           >  1:266837/1‑251 (MQ=255)                                             aaagtttagATCTGCCAAAAGAGGTGGCGGAACGTGTTGATTTCCCCGTGATTTCACATAATGGGCCCGCCGATTTTGCTGCGTTGCGTAAGTTGATGATGAATCGTCAGTAAAATCTAATCATTATCTCAATCAGGCCGGGTTTGCTTTGATGCAGAC‑GGCTTTTTTAGTAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGGGAAAGTCTCAATAAATGCGGTAACGGATAGATTAGGATTGCGga                                                                                                                                                                     <  2:99283/242‑1 (MQ=255)                                                                      ggacgaaggtgttatgtttcccTTGTTTTAAGATAATCTGCTCGGCGATTTTGCTGCGTGGCGTAAATTGATGATGAATCATCTGTAAAATCTATTCAATATCACAATCAGGCCGGGTTGGCTTTGATGCAGCC‑GGCTTTTTTATGAATAAATTAGGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGAATGCGGAGAATAACAACCGACGTTATCATCgcc                                                                                                                                           <  2:193923/232‑3 (MQ=255)                                                                          gAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAAt                                                                                                                                       >  1:76910/1‑251 (MQ=255)                                                                                   gATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAACTTGATGATGACTCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGgag                                                                                                                                                                                                                >  2:254627/1‑169 (MQ=255)                                                                                   gATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGgag                                                                                                                                                                                                                <  1:254627/169‑1 (MQ=255)                                                                                                                                        aTTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTATGAAAAAGATGCAATc                                                                         <  1:197738/251‑1 (MQ=255)                                                                                                                                              gatgaATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTATGAAAAAGATGCAATCTATCg                                                                    <  2:266837/250‑1 (MQ=255)                                                                                                                                                     tagcaGTAAAATCTATTCATTATCTCAATCAGGCGGTGTTTGCTTTTAGGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGGCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTATGAAAAAGATGCAATCTATCGTACTCGc                                                             <  2:60718/247‑1 (MQ=255)                                                                                                                                                       tcaGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAACTTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTATGAAAAAGATGCAATCTATCGTACTCGCACt                                                          >  2:108174/1‑251 (MQ=255)                                                                                                                                                                                   aGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGgc                               >  2:104985/1‑250 (MQ=255)                                                                                                                                                                                             tGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCt                     <  1:149804/250‑1 (MQ=255)                                                                                                                                                                                              gCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGc                   >  1:186907/1‑251 (MQ=255)                                                                                                                                                                                                 tttATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGCAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCgg                 >  2:81501/1‑250 (MQ=255)                                                                                                                                                                                                                 tttttATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCGGCGGAAATTACGTTAGTcc  <  1:104985/250‑1 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                               GCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCC  >  NC_000913/4864‑5321

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑