Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,473,900	C→T	G52R (GGA→AGA)	bdcA ←	c‑di‑GMP‑binding biofilm dispersal mediator protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,473,900	0	C	T	100.0%	66.3 / NA	23	G52R (GGA→AGA)	bdcA	c‑di‑GMP‑binding biofilm dispersal mediator protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/13);  total (10/13)

GGAGCCGATGATTAAGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACA  >  NC_000913/4473658‑4474140                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 ggAGCCGATGATTAAGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTTTGAATACTGCTGTCGCTCTAGTCTCtt                                                                                                                                                                                                                                          <  1:31157/251‑1 (MQ=255)  gAGCCGATGATTAAGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTtg                                                                                                                                                                                                                                         <  2:149169/251‑1 (MQ=255)   ggCCGATGATTAAGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTtgt                                                                                                                                                                                                                                        <  2:244213/250‑1 (MQ=255)                             tCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCAGTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTc                                                                                                                                                                                                                <  1:154087/249‑1 (MQ=255)                                   aTCTGCCGGGCGGATTCAACAGAGGCATGATAAGGAGCATGACTCTTGGTTTTGAAAAGGCGATCCATCTCGTCGGAATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTCCCATCAGGATACCCAATGCGCCGGTCTTACGCACGACATCATTGACCGCGTCTCTGTCAGCACTATCTGTGAATCCTGCTGTAGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCCACCCCGc                                                                                                                                                                                                        >  2:219881/1‑251 (MQ=255)                                                             aTGATAAGGAGCATGAAGATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCATCAGGATATCCAATGCGTCGCTCTTACGAACGACATCGATGACAGCGTCTCTGTCTGCACTATCTGTGAATGCTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTGAGCGGCATCTTTCGTCCCCGCATAGGTGAATCGTACATTGGCCCCAt                                                                                                                                                                              <  1:134266/251‑1 (MQ=255)                                                                          tGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAacg                                                                                                                                                                 >  1:183517/1‑251 (MQ=255)                                                                                      ttGAAAAGGCGATCAATATCGTCGGCATTTCATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCCTCGGTGACGAAACGACGTACGATAGc                                                                                                                                                     >  2:220687/1‑251 (MQ=255)                                                                                      ttGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGc                                                                                                                                                     >  2:71784/1‑251 (MQ=255)                                                                                           aaggcgcgcaatgccgtcgGCACTTATTTTCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCACTGAGCCGCTCTTACGAACGACCTCAATGACAGCGTCTATGTCAGCACTATCTGCGAATACTGCTGTCGCTCTAGTCTCTTGTGCCTGGCGTTTAGCGGAATCTTCCGACCCCGCATAGGTGAATCGTACATTGGCCACATCGGAGACGAAAGGACGCACGATAGTGGCa                                                                                                                                                 >  2:145472/15‑250 (MQ=255)                                                                                                          gtcgGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCCATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGc                                                                                                                                 >  1:97697/1‑251 (MQ=255)                                                                                                                            ggCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTg                                                                                                               <  1:213991/251‑1 (MQ=255)                                                                                                                                            ccAATACCAGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAgc                                                                                               >  2:185087/1‑251 (MQ=255)                                                                                                                                                             caccaGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCtt                                                                              >  1:148593/1‑251 (MQ=255)                                                                                                                                                              cccaGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTGTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTg                                                                             <  1:137854/250‑1 (MQ=255)                                                                                                                                                                          aaTGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTa                                                                 <  1:204793/251‑1 (MQ=255)                                                                                                                                                                                ccgcTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCTGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGATCTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGc                                                           <  2:97697/251‑1 (MQ=255)                                                                                                                                                                                 cgcTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCAGCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCa                                                          <  1:156716/251‑1 (MQ=255)                                                                                                                                                                                          gaacgaCATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAAc                                                 >  1:21908/1‑251 (MQ=255)                                                                                                                                                                                                       gACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACttt                                     <  2:148593/250‑1 (MQ=255)                                                                                                                                                                                                                 ctGTCAACACTATCTGTGAGTACTGCTGTCGCTCTAGTCTCTGGTGCCCTGCGTATAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCTCATCGGTGACGAAACGACGTACGATAGCGGCAACGATACCACGACTGTCACCGAGGATGAGAACTTTCTTGCCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAAGATGAATTACAAATCTGTTTTATCTTTCTGTCAAc                             <  2:185399/248‑1 (MQ=255)                                                                                                                                                                                                                      aGCACTATCTGTGAATACTGCTGTCGCTCTAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTAGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTg                     <  1:245280/251‑1 (MQ=255)                                                                                                                                                                                                                                          ctgTCGCTCTAGTCTCTGGTGCCAGGCGTTTCGCGGCATCTTTCGACCCAGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGAAGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGCTGAGAACTGTCTTACCTGTATAAGCGCCCATAAGGAATCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCACCAGGTTTTGTAATGGTCACTAAAAAACa  >  1:225290/1‑250 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 GGAGCCGATGATTAAGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACA  >  NC_000913/4473658‑4474140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑