Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,445,924	C→T	Q605* (CAG→TAG)	tamB →	translocation and assembly module subunit TamB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,445,924	0	C	T	100.0%	121.7 / NA	40	Q605* (CAG→TAG)	tamB	translocation and assembly module subunit TamB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/18);  total (22/18)

AAACAGTGCCGAAGTGAAAGGCGAGCTGGGGGTAAAAGATCTCAATCTTGATGCCACCATCAACGCGCCGGGGCTGGATAACGCGCTGCCGGGGCTTGGCGGTACAGCGAAAGGGCTGGTGAAAGTACGCGGCACGGTGGAAGCGCCACAACTACTGGCAGATATCACCGCGCGCGGCCTGCGCTGGCAGGAACTTTCCGTGGCGCAGGTTCGCGTGGAAGGCGATATCAAATCCACCGATCAGATCGCCGGGAAACTCGACGTACGCGTTGAGCAAATTTCGCAGCCGGATGTAAATATCAACCTCGTCACCCTGAATGCCAAAGGCAGCGAAAAGCAGCACGAGCTACAGTTGCGGATTCAGGGGGAGC‑CTGTCTCCGGGCAGCTTAATCTGGCAGGAAGTTTTGATCGCAAAGAAGAACGCTGGAAGGGAACTCTTAGCAATACCCGCTTCCAGACGCCGGTTGGCCCGTGGTCGCTG  >  NC_000913/4445683‑4446163                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                 aaacaggtccGAAGTGAAAGGCGAGCTGGGGGTAAAAGAACTCAATCTTGATGCCACCATCAACGCGCCGGGGCTGGATAACGCGCTGCCGGGGTGTGGCGGTACAGCGAAAGGGCTGGTGAAAGTACGCGGCACGGTGGAAGCGCCACAACTACTGGCAGATATCACCGCTCGCGGCCTGCGCTGGCAGGAACTTTCCGTGGCACAGGTTCGCGTGGAAGGCGATATCAAATCCACCGATTAGATCGCCg                                                                                                                                                                                                                                         <  1:167841/243‑1 (MQ=255) aaaCAGGGCCGATGTGAAAGGCGAGCTGGGGGTAAAAGATCTAAATCTTGATGCAACCATCAACGCGCAGGGGCTGGATAACGCGCTGCCGGGGCTTGGAGGTACAGCGAAAGGGCTGGAGAAAGTACGCGGCACGGTGGAAGCGCCACAACTACTGGCAGATATCACCGCGCGCGGCCTGCGCTGGCAGGCACTTTCCGTGGCGCAGGTTCGCGTGGAAGGCGATATCAAATCCCCCGATTAGATCGCCg                                                                                                                                                                                                                                         >  2:230818/1‑251 (MQ=255)  aaCAGTGCCGAAGTGAAAGGCGAGCGGGGGGTAAAAGATCTCAATCGTGATGCCACCATCAACGCGCCGGGGCGGGATAACGCGCTGCCGGGGCTGGGCGGTACAGCGAAAGGGCTGGTGAAAGTACGCGGCACGGTGGAAGCGCCACAACGAGTGGCAGATATCGCCGCGCGCGGCCTGCGCTGGCAGGAACGTTCCGTGGCGCAGGTTCGCGTGGAAGGCGATAGCAAATCCACCGATTAGATCGCCgg                                                                                                                                                                                                                                        <  2:261342/251‑1 (MQ=255)         ccGACGTGAAAGGAGAGCTGGGGGTAAAAGATCTCAATCTTGATGCCACCATCAACGCGCCGGGGCTGGATAACGCGCTGCCGGGGCTTGGCGGTACAGCGAAAGGGCTGGTGAAAGTACGCGGCACGGTGGAAGCGCCACAACTACTGGCAGATATCACCGCGCGCGGCCTGCGCTGGCAGGAACTTTCCGTGGCGCAGGGTCGCGCGGCAGGCGATATAAAATCCACCGATTAGATCGCCgggcaaatc                                                                                                                                                                                                                                 >  2:193985/1‑245 (MQ=255)         ccGAAGTGAAAGGCGAGCTGGGGGTAAAAGATCTCAATCTTGATGCCACCATCAACGCGCCGGGGCTGGATAACGCGCTGCCGGGGCTTGGCGGTACAGCGAAAGGGCTGGTGAAAGTACGCGGCACGGTGGAAGCGCCACAACTACTGGCAGATATCACCGCGCGCGGCCTGCGCTGGCAGGAACTTTCCGTGGCGCAGGTTCGCGTGGAAGGCGATATCAAATCCACCGATTAGATCGCCGGGAAACTc                                                                                                                                                                                                                                 <  2:68711/251‑1 (MQ=255)           gAAGTGAAAGGCGAGCTGGGGGTAAAAGATCTCACTCTTGCTGCCACCATCAACGCGCCGGGGCTGGATAACGCGCTGCCGGGGATTGGCGGTACAGCGAAAGGGCTGGTGAAAGTACGCGGCACGGTGGAAGCGCCACAACTACTGGCAGATATCACCGCGCGCGGCCTGCGCTGGCAGGAACTTTCCGTGGCGCCGGTTCGCGTGGAAGGCGATATCCAATCCACCGATTAGATCGCCAGGAAACTCg                                                                                                                                                                                                                                >  2:148521/1‑250 (MQ=255)                         gCTGGGGGTAAAAGATCTCAATCTTGATGCCACCATCAACGCGCCGGGGCTGGATAACGCGCTGCCGGGGCTTGGCGGTACAGCGAAAGGGCTGGTGAAAGTACGCGGCACGGTGGAAGCGCCACAACTACTGGCAGATATCACCGCGCGCGGCCTGCGCTGGCAGGAACTTTCCGTGGCGCAGGTTCGCGTGGAAGGCGATATCAAATCCACCGATTAGATCGCCGGGACACTCGACGTACGCGGTGAGc                                                                                                                                                                                                                 >  2:257608/1‑251 (MQ=255)                         gCTGGGGGTAAAAGATCTCAATCTTGATGCCACCATCAACGCGCCGGGGCTGGATAACGCGCTGCCGGGGCTTGGCGGTACAGCGAAAGGGCTGGTGAAAGTACGCGGCACGGTGGAAGCGCCACAACTACTGGCAGATATCACCGCGCGCGGCCTGCGCTGGCAGGAACTTTCCGTGGCGCAGGTTCGCGTGGAAGGCGATATCAAATCCACCGATTAGATCGCCGGGAAACTCGACGTACGCGTTGAGc                                                                                                                                                                                                                 >  1:240818/1‑251 (MQ=255)                           tGGGGGTAAAAGATCTCAATCTTGATGCCACCATCAACGCGCCGGGGCTGGATAACGCGCTGCCGGGGCTTGGCGGTACAGCGAAAGGGCTGGTGAAAGTACGCGGCACGGTGGAAGCGCCACAACTACTGGCAGATATCACCGCGCGCGGCCTGCGCTGGCAGGAACTTTCCGTGGCGCCGGTTCGCGTGGAAGGCGATATCAAATCCACCGATTAGATCGCCGGGCAACTCGAc                                                                                                                                                                                                                              >  2:99815/1‑236 (MQ=255)                           tGGGGGTAAAAGATCTCAATCTTGATGCCACCATCAACGCGCCGGGGCTGGATAACGCGCTGCCGGGGCTTGGCGGTACAGCGAAAGGGCTGGTGAAAGTACGCGGCACGGTGGAAGCGCCACAACTACTGGCAGATATCACCGCGCGCGGCCTGCGCTGGCAGGAACTTTCCGTGGCGCAGGTTCGCGTGGAAGGCGATATCAAATCCACCGATTAGATCGCCGGGAAACTCGAc                                                                                                                                                                                                                              <  1:99815/236‑1 (MQ=255)                            gggggTAAAAGATCTCAATCTTGATGCCACCATCAACGCGCCGGGGCTGGATAACGCGCTGCCGGGGCTTGGCGGTACAGCGAAAGGGCTGGTGAAAGTACGCGGCACGGTGGAAGCGCCACAACTACTGGCAGATATCACCGCGCGCGGCCTGCGCTGGCAGGAACTTTCCGTGGCGCAGGTTCGCGTGGAAGGCGATATCAAATCCACCGATTAGATCGCCGGGAAACTCGACGTACGCGTTGAGCaa                                                                                                                                                                                                               <  2:194476/250‑1 (MQ=255)                                     aGATCTCAATCTTGATGCCACCATCCACGCGCCGGGGCTGGATAACGCGCTGCCGGGGCTTGGCGGTACAGCGAAAGGGCTGGTGAAAGTACGCGGCACGGTGGAAGCGCCACAACTACTGGCAGATATCACCGCGCGCGGCCTGCGCCGGCAGGAACTTTCCGTGGCGCAGGTTCGCGTGGAAGGGGATATCAAATCCACCGATTAGATCTCCGGGAGACTCGACGTACGCGGTGAGCAATTTTagcagc                                                                                                                                                                                                     >  2:18219/1‑251 (MQ=255)                                           cAATCTTGATGCCACCATCAACGCGCCGGGGCTGGATAACGCGCTGCCGGGGCTTGGCGGTACAGCGAAAGGGCTGGTGAAAGTACGCGGCACGGTGTAAGCGCCACAACTACTGGCAGATATCACCGCGCGCGGCCTGCGCTGGCAGGAACTTTCCGTGGCGCAGGTTCGCGTGGAAGGCGATATCAAATCCACCGATTAGATCGCCGGGAAACTCGACGTACGCGTTGAGCAAATTTCGCAGCCg                                                                                                                                                                                                   <  1:62716/247‑1 (MQ=255)                                               cTTGATGCCACCATCAACGCGCCGGGGCTGGATAACGCGCTGCCGGGGCTTGACGGTACAGCGCAAGGGCTGGTGAAAGTACGCGGCACGGTGGAAGCGCCACAACTACTGGCAGATATCACCGCGCGCGGCCTGCGCTGGCAGGAACTTTCCGTGGCGCAGGCTCGCGTGAACGGCCATATCACATCCACCGATTAGATCGCCGGGAACCTCGACGACCGCGTTGAGCAAATTTCGCCGCCGGATgaca                                                                                                                                                                                            >  1:263235/1‑247 (MQ=255)                                                          caTCAACGCGCCGGGGCTGGATAACGCGCTGCCGGGGCTTGGCGGTACAGCGAAAGGGCTGGTGAAAGTACGCGGCACGGTGGAAGCGCCACAACTACTGGCAGATATCACCGCGCGCGGCCTGCGCTGGCAGGAACTTTCCGTGGCGCAGGTTCGCGTGGAAGGCGATATCAAATCCACCGATTAGATCGCCGGGAAACTCGACGTACGCGTTGAGCAAATTTCGCAGCCGGATGTAAATATCAACCTCg                                                                                                                                                                                <  2:90725/251‑1 (MQ=255)                                                                 gcgcCGGGGCTGGATAACGCGCTGCCGGGGCTTGGCGGTACAGCGAAAGGGCTGGTGAAAGTACGCGGCACGGTGGAAGCGCCACAACTACTGGCAGATATCACCGCGCGCGGCCTGCGCTGGCAGGAACTTTCCGTGGCGCAGGTTCGCGTGGAAGGCGATATCAAATCCACCGATTAGATCGCCGGGAAACTCGACTTACGCGTTGAGCAAATTTCGCAGCCGGATGTAAATATCAACCTCGTCACCCt                                                                                                                                                                         <  1:259560/251‑1 (MQ=255)                                                                      ggggCTGGATAACGCGCTGCCGGGGCGTGGCGGTACAGCGAAAGGGCTGGTGAAAGTACGCGGCACGGTGGAAGCGCCACAACTACTGGCAGATATCACCGCGCGCGGCCTGCGCTGGCAGGAACTTTCCGTGGCGCAGGTTCGCGTGGAAGGCGATATCAAATCCACCGATTAGATCGCCGGGAAACTCGACGTACGCGTTGAGCAAATTTCGCAGCCGGATGTAAATATCAACCTCGTCACCCTGAATg                                                                                                                                                                    <  2:196643/251‑1 (MQ=255)                                                                           tGGATAACGCGCTGCCGGGGCTTGGCGGTACAGCGAAAGGGCTGGTGAAAGTACGCGGCACGGTGGAAGCGCCACAACTCCTGGCAGATATCACCGCGCGCGGCCTGCGCTGGCAGGAACTTTCCGTGGCGCAGGTTCGCGTGGAAGGCGATATCAAATCCACCGATTAGATCGCCGGGAAACTCGACGTACGCGTTGAGCAAATTTCGCAGCCGGATGTCAATATCAACATCGTCACCCTGAATGCCaa                                                                                                                                                                >  2:139113/1‑250 (MQ=255)                                                                                           ggggCTTGGCGGTACAGCGAAAGGGCTGGTGAAAGTACGCGGCACGGTGGAAGCGCCACAACTACTGGCAGATATCACCGCGCGCGGCCTGCGCTGGCAGGAACTTTCCGTGGCGCAGGTTCGAGTGGCAGGCGATATCAAATCCACCGATTAGATCGCCGGGAAACTCGACGTCCGCGTTGAGCAAATTTCGCAGCCGGATGTAAATATCAACCTCGTCACCCTGAATGCCAAAGGCAGCGAAAagcagc                                                                                                                                               >  1:127680/1‑251 (MQ=255)                                                                                                 tGGCGGTACAGCGAAAGGGCTGGTGAAAGTACGCGGCACGGTGGAAGCGCCACAACTACTGGCAGATATCACCGCGCGCGGCCTGCGCTGGCAGGAACTTTCCGTGGCGCAGGTTCGCGTGGAAGGCGATATCAAATCCACCGATTAGATCGCCGGGAAACTCGACGTACGCGTTGAGCAAATTTCGCAGCCGGATGTAAATATCAACCTCGTCACCCTGAATGCCAAAGGCAGCGAAAAGCAGCACGAGc                                                                                                                                         >  1:184569/1‑251 (MQ=255)                                                                                                       tACAGCGAAAGGGCTGGTGAAAGTACGCGGCACGGTGGAAGCGCCACAACTACTGGCAGATATCACCGCGCGCGGCCTGCGCTGGCAGGAACTTTCCGTGGCGCAGGTTCGCGTGGAAGGCGATATCAAATCCACCGATTAGATCGCCGGGAAACTCGACGTACGCGTTGAGCAAATTTCGCAGCCGGATGTAAATATCAACCTCGTCACCCTGAATGCCAAAGGCAGCGAAAAGCAGCACGAGCTACAGt                                                                                                                                   >  1:40542/1‑251 (MQ=255)                                                                                                                     tGGTGAAAGTACGCGGCACGGTGGAAGCGCCACAACTACTGGCAGATATCACCGCGCGCGGCCTGCGCTGGCAGGAACTTTCCGTGGCGCAGGTTCGCGTGGAAGGCGATATCAAATCCACCGATTAGATCGCCGGGAAACTCGACGTACGCGTTGAGCAAATTTCGCAGCCGGATGTAAATATCAACCTCGTCACCCTGAATGCCAAAGGCAGCGAAAAGCAGCACGAGCTACAGTTGCGGATTCAgggg                                                                                                                     >  1:210986/1‑251 (MQ=255)                                                                                                                                  cgGCACGGTGGAAGCGCCACAACTACTGGCAGATATCACCGCGCGCGGCCTGCGCTGGCAGGAACTTTCCGTGGCGCAGGTTCGCGTGGAAGGCGATATCAAATCCACCGATTAGATCGCCGGGAAACTCGACGTACGCGTTGAGCAAATTTCGCAGCCGGATGTAAATATCAACCTCGTCACCCTGAATGCCAAAGGCAGCGAAAAGCAGCACGAGCTACAGTTGCGGATGCAGGGGGAGC‑CTGTCTCCg                                                                                                       >  1:78020/1‑251 (MQ=255)                                                                                                                                   ggCACGGTGGAAGCGCCACAACTACTGGCAGATATCACCGCGCGCGGCCTGCGCTGGCAGGAACTTTCCGTGGCGCAGGTTCGCGTGGAAGGCGATATCAAATCCACCGATTAGATCGCCGGGAAACTCGACGTACGCGTTGAGCAAATTTCGCAGCCGGATGTAAATATCAACCTCGTCACCCTGAATGCCAAAGGCAGCGAAAAGCAGCACGAGCTACAGTTGCGGATTCAGGGGGAGC‑CTGTCTCCgg                                                                                                      >  2:116846/1‑251 (MQ=255)                                                                                                                                      aCGGTGGAAGCGCCACAACTACTGGCAGATATCACCGCGCGCGGCCTGCGCTGGCAGGAACTTTCCGTGGCGCAGGTTCGCGTGGAAGGCGATATCAAATCCACCGATTAGATCGCCGGGAAACTCGACGGACGCGTTGAGCAAATTTCGCAGCCGGATGTAAATATCAACCTCGTCACCCTGAATGCCAAAGGCAGCGAAAAGCAGCACGAGCTACAGTGGCGGAGTCAGGGGGAGC‑CTGTCTCCGGGCa                                                                                                   <  2:184569/251‑1 (MQ=255)                                                                                                                                             aaGCGCCACAACTACTGGCAGATATCACCGCGCGCGGCCTGCGCTGGCAGGAACTTTCCGTGGCGCAGGTTCGCGTGGAAGGCGATATCAAATCCACCGATTAGATCGCCGGGAAACTCGACGTACGCGTTGAGCAAATTTCGCAGCCGGATGTAAATATCAACCTCGTCACCCTGAATGCCAAAGGCAGCGAAAAGCAGCACGAGCTACAGTTGCGGATTCAGGGGGAGC‑CTGTCTCCGGGCAGCTTAAt                                                                                            <  1:116846/251‑1 (MQ=255)                                                                                                                                                  ccACAACTACTGGCAGATATCACCGCGCGCGGCCTGCGCTGGCAGGAACTTTCCGGTGCGCAGTTTCGCGTGGAAGGCGATATCAAATCCACCGATTAGATCGCCGGGAAACTCGACGTACGCGTTGAGCAAATTTCGCAGCCGGATGTAAATATCAACCTCGTCACCCTGAATGCCAAAGGCAGCGAAAAGCAGCACGAGCTACAGTGGCGGATTCAGTGGGATC‑CTGTCTCCGTGCATCTTAATCTg                                                                                         <  2:158933/249‑1 (MQ=255)                                                                                                                                                                    atCACCGCGCGCGGCCTGCGCTGGCAGGAACTTTCCGTGGCGCAGGTTCGCGTGGAAGGCGATATCAAATCCACCGATTAGATCGCCGGGAAACTCGACGTACGCGTTGAGCAAATTTCGCAGCCGGATGTAAATATCa                                                                                                                                                                                      <  1:227543/139‑1 (MQ=255)                                                                                                                                                                    atCACCGCGCGCGGCCTGCGCTGGCAGGAACTTTCCGTGGCGCAGGTTCGCGTGGAAGGCGATATCAAATCCACCGATTAGATCGCCGGGAAACTCGACGTACGCGTTGAGCAAATTTCGCAGCCGGATGTAAATATCa                                                                                                                                                                                      >  2:227543/1‑139 (MQ=255)                                                                                                                                                                                ggCCTGCGCTGGCAGGAACTTTCCGTGGCGCAGGTGCGCGTGGAAGGCGATATCAAATCCACCGATTAGATCGCCGGGAAACTCGACGTACGCGTTGAGCAAATTTCGCAGCCGGATGTAAATATCAACCTCGTCACCCTGAATGCCAAAGGCAGCGAAAAGCAGCACGAGCTACAGTTGCGGATTCAGGGGGAGC‑CTGTCTCCGGGCAGCTTAATCTGGCAGGAAGTTTTGATCGCAAAGAAGAACGCTg                                                         <  2:210986/251‑1 (MQ=255)                                                                                                                                                                                     ggggtGGCAGGAACATCCCGGGGCGCAGGTGCGCGTGGAAGGCGATATCAAATCCACCGATTAGATCGCCGGGAAACGCGACGTACGCGTTGAGCAAATTACGCAGCCGGATGGAAATATCAACCTCGGCACCCCGAATGCCAAAGGCAGCGAAAAGCAGCACGAGCTACAGTTGCGGATTCAGGGGG‑GCGCTGGCTCCGGGCATCTTAATCTGGCAGGAAGGTTTGAGCGCAAAGAAGAGCGCTGGAAg                                                     <  2:263235/246‑1 (MQ=255)                                                                                                                                                                                      cgcTTGCATGAACTTTCCGTGGCGCAGGTTCGCGTTGAAGGCGATATCAAATCCACCGATTAGATCGCCGGGAAACTCGACGTACGCGTTGAGCAAATTTCGCAGCCGGATGTAAATATCAACCTCGTCACCCTGAACGCCAAAGGCAGCGAAAAGCAGCACGAGCTGCAGTTGCGGATTCAGGGGAAGC‑CTGGCTCCGGGCAGCGTAAGCGTTCAGAAAGTTGTGAGGTCAAAGAAGAACTCTGGAAggg                                                   <  2:239217/251‑1 (MQ=255)                                                                                                                                                                                           gCAGGAACTTTCCGTGGCGCAGGTTCGCGTGGAAGGCGATATCACATCCACCGATTAGATCGCCGGGCAACTCGACGTACGCGTTGAGCAAATTTCGCAGCCGGATGTAAATATCAACCTCGTCACCCTGAATGCCAAAGGCAGCGAAAAGCAGCACGAGCTACAGTTGCGGATTCAGGGGGAGC‑CTGTCTCCGGGCAGCTTAATATGGCAGGAAGTTTTGATCGCAAAGAAGAACGCTGGAAGGGAActc                                              >  2:105560/1‑251 (MQ=255)                                                                                                                                                                                                      ccGTGGCGCAGGGTCGCGTGGAAGGAGATATCACATCCACCGATTAGATCGCCGGGAAACTCGACGTACGCGTTGAGCAAATTTCGCAGCCGGATGTAAATATCAACCTCGTCACCCTGAATGCCAAAGGCAGCGAAAAGCAGCACGAGCTACAGTTGCGGATTCAGGGGGAGA‑CTGTCCCCGGGCAGCTTAATCTGGCAGGAAGTTGTGATCGCAAAGAAGAACGCTGGAAGGGAGCTCTTAGCAATAcc                                   >  2:199960/1‑251 (MQ=255)                                                                                                                                                                                                        gTGGCGCAGGTTCGCGTGGAAGGCGATATCAAATCCACCGATTAGATCGCCGGGAAACTCGACGTACGCGTTGAGCAAATTTCGCAGCCGGATGTAAATATCAACCTCGTCACCCTGAATGCCAAAGGCAGCGAAAAGCAGCACGAGCTACAGTTGCGGATTCAGGGGGAGC‑CTGTCTCCGGGCAGCTTAATCTGGCAGGAAGTTTTGATCGCAAAGAAGAACGCTGGAAGGGAACTCTTAGCAATACCCg                                 >  2:232221/1‑251 (MQ=255)                                                                                                                                                                                                             gcAGGTTCGCGTGGAAGGCGATATCAAATCCACCGATTAGATCGCCGGGAAACTCGACGTACGCGTTGAGCAAATTTCGCAGCCGGATGTAAATATCAACCTCGTCACCCTGAATGCCAAAGGCAGCGAAAAGCAGCACGAGCTACAGTTGCGGATTCAGGGGGAGC‑CTGTCTCCGGGCAGCTTAATCTGGCAGGAAGTTTTGATCGCAAAGAAGAACGCTGGCAGGGAACTCTTAGCAATACCCgagctc                            >  2:64519/1‑246 (MQ=255)                                                                                                                                                                                                             gcAGGGTCGCGTGGAAGGCGATATCAAATCCACCGATTAGATCGCCGGGAAACTCGACGTACGCGTTGAGCAAATTTCGCAGCCGGATGTAAATATCAACCTCGTCACCCTGAATGCCAAAGGCAGCGAAAAGCAGCACGAGCTACAGTTGCGGATTCAGGGGGAGC‑CTGTCTCCGGGCAGCTTAATCTGGCAGGAAGTTTTGATCGCAAAGAAGAACGCTGGAAGGGAACTCTTAGCAATACCCg                                 <  1:64519/246‑1 (MQ=255)                                                                                                                                                                                                                                 atatCAAATCCACCGATTAGATCGCCGGGAAACTCGACGTACGCGTTGAGCAAATTTCGCAGCCGGATGTAAATATCAACCTCGTCACCCTGAATGCCAAAGGCAGCGAAAAGCAGCACGAGCTACAGTTGCGGATTCAGGGGGAGC‑CTGTCTCCGGGCAGCTTAATCTGGCAGGAAGTTTTGATCGCAAAGAAGAACGCTGGAAGGGAACTCTTAGCAATACCCGCTTCCAGACGCCGGTTGGCCCGTgg        >  2:137174/1‑251 (MQ=255)                                                                                                                                                                                                                                   atCAAATCCACCGATTAGATCGCCGGGAAACTCGACGTACTCGTTGAGCAAAATTCGCAGCCGGAGGTAAATATCAACCTCGTCACCCTGAATGCCAAAGGCAGCGAAAAGCAGCACGAGCTACAGTTGCGGATTCAGGGGGAGC‑CTGTCTCCGGGCAGCTTAATCTGGCAGGAAGTTTTGATCGCAAAGAAGAACGCTGGAAGGGAACTCTTAGCAATACCCGCTTCCAGACGCCGGTTGGCCCGTGGTc      <  1:148521/251‑1 (MQ=255)                                                                                                                                                                                                                                       aaTCCACCGATTAGATCGCCGGGAAACTCGACGTACGCGTTGAGCAAATTTCGCAGCCGGATGTAAATATCAACCTCGTCACCCTGAATGCTAAAGGCAGCGAAAAGCAGCACGAGCTACAGTTGCGGATTCAGGGGGAGC‑CTGTCTCCGGGCAGCTTAATCTGGCAGGAAGTTTTGATCGCAAAGAAGAACGCTGGAAGGGAACTCTTAGCAATACCCGCTTCCAGACGCCGGTTGGCCCGTGGTCGCTg  >  2:132724/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                 AAACAGTGCCGAAGTGAAAGGCGAGCTGGGGGTAAAAGATCTCAATCTTGATGCCACCATCAACGCGCCGGGGCTGGATAACGCGCTGCCGGGGCTTGGCGGTACAGCGAAAGGGCTGGTGAAAGTACGCGGCACGGTGGAAGCGCCACAACTACTGGCAGATATCACCGCGCGCGGCCTGCGCTGGCAGGAACTTTCCGTGGCGCAGGTTCGCGTGGAAGGCGATATCAAATCCACCGATCAGATCGCCGGGAAACTCGACGTACGCGTTGAGCAAATTTCGCAGCCGGATGTAAATATCAACCTCGTCACCCTGAATGCCAAAGGCAGCGAAAAGCAGCACGAGCTACAGTTGCGGATTCAGGGGGAGC‑CTGTCTCCGGGCAGCTTAATCTGGCAGGAAGTTTTGATCGCAAAGAAGAACGCTGGAAGGGAACTCTTAGCAATACCCGCTTCCAGACGCCGGTTGGCCCGTGGTCGCTG  >  NC_000913/4445683‑4446163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑