Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,440,768	G→A	L150L (CTG→TTG)	paeA ←	putative cadaverine/putrescine exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,440,768	0	G	A	100.0%	102.0 / NA	33	L150L (CTG→TTG)	paeA	putative cadaverine/putrescine exporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/15);  total (18/15)

ATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAACACGCCCGCCAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTAT‑GTTCGCCAGGCCGTTGAAGAACCACACCAGCGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGCAAAGCCACAGCTTCTGGCGCAATCATACCGATGCGTTTCGGGGTTAAATCCGCAAACAGGATAAACATGCCAGTCACTAACGAGAAAGAGAGAATAAAGCTCAGTTGCTCAGAGAGCTCTGCCGACATATAGCGGGAGAACAGGCTGTGAAAAGCTGGAGAAAATGCCGCATCACCGACGATACCGCCG  >  NC_000913/4440526‑4441003                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                            aTCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAACACGCCCGCCAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTAT‑GTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGc                                                                                                                                                                                                                                     >  1:123318/1‑251 (MQ=255)         ggAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAACACGCCCGCCAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTAT‑GTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTAc                                                                                                                                                                                                                             >  1:123034/1‑251 (MQ=255)               cAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAACACGCCCGCCAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTAT‑GTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGc                                                                                                                                                                                                                         >  1:155598/1‑249 (MQ=255)               cAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAAAGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAACACGCCCGCCAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGGAAATACGGAAGATTAT‑TTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCCAACGTACAGGc                                                                                                                                                                                                                         >  2:47677/1‑249 (MQ=255)                                 ccggcgtggtctttGAGGACGGAACGGTCCGGGATTCCAGCTCAAAGCCGTTCTCAATCAGTGCGGTTTCCTTTTTACGTAACACGCCCGCCAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTGTGAAAATACGTAAGATTAT‑GTTCGCCAGGCCGTTGAAGAACCACTCCAACGGTGTGCAAACGGACAGTCAGAAGCGCATCGGGTttatg                                                                                                                                                                                                     <  2:47535/239‑1 (MQ=255)                                              ggAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAACACGCCCGCCAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTAT‑GTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGCAAAGCca                                                                                                                                                                                        <  2:48817/250‑1 (MQ=255)                                                                     ccAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACTTAACACGCCCGCCAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTAT‑GTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGCAAAGCCACAGCTTCTGGCGCAATCATAcc                                                                                                                                                                  <  1:261969/250‑1 (MQ=255)                                                                       aGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAACACGCCCGCCAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTAT‑GTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGCAAAGCCACAGCTTCTGgc                                                                                                                                                                             <  1:92048/237‑1 (MQ=255)                                                                       aGCTCAAAGACGTTTTAAATCAGCTCGTGTTCCTGTTTACGTAACACGCCCGCCAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTAT‑GTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGCAAAGCCACAGCTTCTGgc                                                                                                                                                                             >  2:92048/1‑237 (MQ=255)                                                                              agattttttCAATCAGCTCGTGTTCCTGTTTACGTAACCCGCCCGCCAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTAT‑GTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGCAAAGCCACAGCTTCTGGCGCAATCATACCGATGCGTTTc                                                                                                                                                        <  2:81450/246‑1 (MQ=255)                                                                                          gcAGCTCGTGTTCGTGTTCTCGTAATGGGCCCGCCAGCGCACCGGCTTCCACTACCGCGTGGAGGTCTTCAGAAGTGATGTCATCTTTACGGTCCATTGGCAGTTTGAAAATACGGGAGATTATTGTT‑GTCTTGCCGTTGAAGAACCACGCCAACTGGGTGCAAAGGTGTAGGGAGACGGGTATGGGGTTGATGATACGCAGATCCACAGCTGCTGGCGCAATCATACCGATGCGTTTCGGGGTTAAATcc                                                                                                                                            <  1:240739/250‑1 (MQ=255)                                                                                                               gTAACACGCGCGCCAGCGCACCGGCTTCCACTACCGCGTAGATGTCATAAAAAGTGATGTCATCTTTACGTACCATTGGCCGTTTGAAAATACGGAAGATTAT‑GTTCGCCAGGCCGTTGAAGAACCACGCCAAAGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGCTGCTACGCAAAGCCCCAGCTTCTGGCGCAATCATACCGATGCGTTTCGGGGTTAAATCCGCAAACAGGATAAAGATGcc                                                                                                                        >  1:129294/1‑250 (MQ=255)                                                                                                                        ccgccAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTAT‑GTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGCAAAGCCACAGCTTCTGGCGCAATCATACCGATGCGTTTCGGGGTTAAATCCGCAAACAGGATAAACATGCCAGTCACTAAc                                                                                                              >  1:165696/1‑251 (MQ=255)                                                                                                                            cAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTAT‑GTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGCAAAGCCACAGCTTCTGGCGCAATCATACCGATGCGTTTCGGGGTTAAATCCGCAAACAGGATAAACATGCCAGTCACTAACg                                                                                                             >  2:201857/1‑248 (MQ=255)                                                                                                                             aGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATATTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTAT‑GTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGCAAAGCCACAGCTTCTGGCGCAATCATACCGATGCGTTTCGGGGTTAAATCCGCAACCAGGATAAACATGCCAGTCACTAACgaga                                                                                                          >  2:268766/1‑250 (MQ=255)                                                                                                                              gcgcACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTAT‑GTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGCAAAGCAACAGTTTCTGGCTCAATCATACCGATGCGTTTCGGGGTTAAAGCCGCAAACAGGAGAAACATGCCAGCCACTAACGAGaaa                                                                                                        <  2:187204/251‑1 (MQ=255)                                                                                                                                 cACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTAT‑GTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGCAAAGCCACAGCTTCTGGCGCAATCATACCGATGCGTTTCGGGGTTAAATCCGCAAACAGGATAAACATGCCAGTCACTAACGAGAAag                                                                                                       >  1:263029/1‑249 (MQ=255)                                                                                                                                      gCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTCCCATTGGCAGTTTGAAAATACGGAAGATTAT‑GTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACACAAAGCCACAGCTTCTGGCGCAATCATACCGATGCGCTTCGGGGATAAATCCGCAAAAAGGATAAACATGCCAGTCACTAACGAGACAGAGAGaa                                                                                                 >  1:106443/1‑250 (MQ=255)                                                                                                                                                ccGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTAT‑GTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGCAAAGCCACAGCTTCTGGCGCAATCATACCGATGCGTTTCGGGGTTAAATCCGCAAACAGGATAAACATGCCAGTCACTAACGAGAAAGAGAGAATAAAGCTCAGt                                                                                      <  2:155598/251‑1 (MQ=255)                                                                                                                                                        aTGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAACATAAGGAAGATTAT‑GTTCGCCAAGCCGTTGAAGAACCACACCAACGGGGGGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGAAAAGCCACAGCTTATGGCGCAATCATACAGATGCGTTTCGGGGTTACATCTGCAAACAGGATAAACATGCCAGTCACTAACGAGAAAGAGAGAATAAAGCTCAGTTGCTc                                                                                 >  1:26791/1‑248 (MQ=255)                                                                                                                                                            catcaGACGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTAT‑GTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGCTGATGATACGCAAAGCCACAGCTTCTGGCGCAATCATACCGATGCGTTTCGGGGTTAAATCCGCAAACAGGATAAACATGCCAGTCACTAACGAGAAAGAGAGAATAAAGCTCAGTTGCTCAGAGAGc                                                                          >  1:207151/1‑251 (MQ=255)                                                                                                                                                               caGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTAT‑GTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGCAAAGCCACAGCTTCTGGCGCAATCATACCGATGCGTTTCGGGGTTAAATCCGCAAACAGGATAAACATGCCAGTCACTAACGAGAAAGAGAGAATAAAGCTCAGTTGCTCAGAGAGctct                                                                       <  2:209809/251‑1 (MQ=255)                                                                                                                                                               caGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTAT‑GTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGCAAAGCCACAGCTTCTGGCGCAATCATACCGATGCGTTTCGGGGTTAAATCCGCAAACAGGATAAACATGCCAGTCACTAACGAGAAAGAGAGAATAAAGCTCAGTTGCTCAGAGAGctc                                                                        <  1:53704/250‑1 (MQ=255)                                                                                                                                                               caGAAGTGATGTCATCTTTACGAACCATTGGCCGTTTGAAAATACCGAAGATTAT‑GTTCGCCAGGCCGTTGAAGAACCACACAAACGGGGTGCAAACGAACAGGCAGAAGAGCATCGGGTTGATGATACGCAAAGCCACAGCTTCTGGCGCAATCATACCGATGCGTTTCGGGGTTAAATCCGCAAACAGGATAAACATGCCAGTCACTAACGAGAACGAGAGAATAAAGCTCAGTTGCTCAGAGAGctct                                                                       >  1:38907/1‑251 (MQ=255)                                                                                                                                                                aGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTAT‑GTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGCAAAGCCACAGCTTCTGGCGCAATCATACCGATGCGTTTCGGGGTTAAATCCGCAAACAGGATAAACATGCCAGGCACTAACGAGAAAGAGAGAATAAAGCTCAGTGGCTCAGAGAGCTCTg                                                                      <  2:118111/251‑1 (MQ=255)                                                                                                                                                                  aaGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTAT‑GTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGCAAAGCCACAGCTTCTGGCGCAATCATACCGATGCGTTTCGGGGTTAAATCCGAAAACAGGATAAACATGCCAGTCACTAACGAGAAAGAGAGAATAAAGCTCAGTTGCTCAGAGAGCTCTGcc                                                                    >  2:131469/1‑251 (MQ=255)                                                                                                                                                                                      aCCATTGGCAGTTTGAAAATACGGAAGATTAT‑GTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGCAAAGCCACAGCTTCTGGCGCAATCATACCGATGCGTTTCGGGGTTAAATCCGCAAACAGGATAAACATGCCAGTCACTAACGAGAAAGAGAGAATAAAGCTCAGTTGCTCAGAGAGCTCTGCCGACATATAGCGGGAGAACAg                                                <  1:201857/251‑1 (MQ=255)                                                                                                                                                                                         aTTGGCAGTTTGAAAATACGGAAGATTAT‑GTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGCAAAGCCACAGCTTCTGGCGCAATCATACCGATGCGTTTCGGGGTTAAATCCGCAAACAGGATAAACATGCCAGTCACTAACGAGAAAGAGAGAATAAAGCTCAGTTGCTCAGAGAGCTATGCCGACATATAGCGGGAGAACAGGCt                                             >  2:82824/1‑251 (MQ=255)                                                                                                                                                                                              cAGTTTGAAAATACGGGAGATAAT‑GGTCGTCAGGCCGTTGAAGAACCACACCAACGGTGTGCAAACGTACAGGCAGAAGCGCATTGTGTTGATGATTCGCAAAGCCACAGCTTCTGGCGCAATCATACCGATTCGTTGCGGTGTTAAAGCCGCATACAGTATAAACATGCCAGTCACTAACGAGAAAGAGAGAAGAAATCTCAGTTGCGCAGAGAGTGCTTCCGACATATAggctgcgaacaggatgggaa                                        <  2:129391/251‑20 (MQ=255)                                                                                                                                                                                              cAGTTTGAAAATACGGAAGATTAT‑GTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGCAAAGCCACAGCTTCTGGCGCAATCATACCGATGCGTTTCGGGGTTAAATCCGCAAACAGGATAAACATGCCAGTCACTAACGAGAAAGAGAGAATAAAGCTCAGTTGCTCAGAGAGCTCTGCCGACATATAGCGGGAGAACAGGCTGTGaa                                        <  2:235430/251‑1 (MQ=255)                                                                                                                                                                                                      aaaTACGGAAGATTAT‑GTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGCAAAGCCACAGCTTCTGGCGCAATCATACCGATGCGTTTCGGGGTTAAAGCCGCAAACAGGATAAACATGCCAGTCACTAACGAGAAAGAGAGAATAAAGCTCAGTTGCTCAGAGAGCTCTGCCGACATATAGCGTGAGAACAGGCTGTGAAAAGCTGga                                <  2:165696/251‑1 (MQ=255)                                                                                                                                                                                                          aCGGAAGATTAT‑GTTCGCCAGGCCGTTGAAGAACCACACCAACGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGCAAAGCCACAGCTTCTGGCGCAATCATACCGATGCGTTTCGGGGTTAAATCCGCAAACAGGATAAACATGCCAGTCACTAACGAGAAAGAGAGACTAAAGCTCAGTTGCTCAGAGAGCTCTGCCGACATATAGCGGGAGAACAGGCTGTGAAAAGCTGGAGaa                             >  1:247550/1‑250 (MQ=255)                                                                                                                                                                                                                                        agaaCCACACCAACGGGGTGCAAACGTACAGGCAGCAGCGCATCGGGTTGCTGATACGCAAAGCCACAGCTTCTGGCGCAATCATACCGATGCGTTTCGGGGTTAAATCCGCAAACAGGATAAACATGCCAGTCACTAACGAGAAAGAGAGAATAAAGCTCAGTTGCTCAGAGAGCTCTGCCGACATCTAGCGGGAGAACAGGCTGTGAAAAGCTGGAGAAAATGCCGCATCACCGCCGATAccgccg  >  2:201299/1‑248 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                            ATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAACACGCCCGCCAGCGCACCGGCTTCCACTACCGCGTAGATGTCATCAGAAGTGATGTCATCTTTACGTACCATTGGCAGTTTGAAAATACGGAAGATTAT‑GTTCGCCAGGCCGTTGAAGAACCACACCAGCGGGGTGCAAACGTACAGGCAGAAGCGCATCGGGTTGATGATACGCAAAGCCACAGCTTCTGGCGCAATCATACCGATGCGTTTCGGGGTTAAATCCGCAAACAGGATAAACATGCCAGTCACTAACGAGAAAGAGAGAATAAAGCTCAGTTGCTCAGAGAGCTCTGCCGACATATAGCGGGAGAACAGGCTGTGAAAAGCTGGAGAAAATGCCGCATCACCGACGATACCGCCG  >  NC_000913/4440526‑4441003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑