Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,414,192	G→A	intergenic (+1/‑83)	yjfC → / → aidB	putative acid‑‑amine ligase YjfC/putative acyl‑CoA dehydrogenase AidB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,414,192	0	G	A	100.0%	135.9 / NA	42	intergenic (+1/‑83)	yjfC/aidB	putative acid‑‑amine ligase YjfC/putative acyl‑CoA dehydrogenase AidB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/25);  total (17/25)

CGCGAAGGCGGCAACGTCACCATTTTTGACGGCAAGAATAACGTTGTTGACCACGCTGATGGTGATTACGCCGATGAACCGATGATCTACCAGGCGTTTCAACCTCTGCCGCGGTTTGGCGATAGCTACACACTCATCGGTAGCTGGATTGTC‑GATGATGAAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGG  >  NC_000913/4413946‑4414431                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                cgcgAAGGCGTCAACGTCACCATTTTTGACGGCAAGAATAACGTTGTTGACCACGCTGATGGTGATTACGCCGATGAACCGATGATCTACCAGGCGTTTCAACCTCTGCCGCGGTTTGGCGATAGCTACACACTCATCGGTAGCTGGATTGTC‑GATGATGAAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCGCACGTTTCGTTCCGCATTACATTGCTGGATAAAAATg                                                                                                                                                                                                                                             <  1:12434/251‑1 (MQ=255)   cgAAGGCGGCAACGTCACCATTTTTGACGGCAAGAATAACGTTGTTGACCACGCTGATGGTGATTACGCCGATGAACCGATGATCTACCAGGCGTTTCAACCTCTGCCGCGGTTTGGCGATAGCTACACACTCATCGGTAGCTGGATTGTC‑GATGATGAAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAAAATGtt                                                                                                                                                                                                                                           >  1:121916/1‑251 (MQ=255)   cgAAGGCGGCAACGTCACCATTTTTGACGGCAAGAATAACGTTGTTGACCACGCTGATGGTGATTACGCCGATGAACCGATGATCTACCAGGCGTTTCAACCTCTGCCGCGGTTTGGCGATAGCTACACACTCATCGGTAGCTGGATTGTC‑GATGATGAAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAAAATGtt                                                                                                                                                                                                                                           <  2:66590/251‑1 (MQ=255)        gcggcAACGTCACCATTTTTGACGGCAAGAATAACGTTGTTGACCACGCTGATGGTGATTACGCCGATGAACCGATGATCTACCAGGCGTTTCAACCTCTGCCGCGGTTTGGCGATAGCTACACACTCATCGGTAGCTGGATTGTC‑GATGATGAAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCGGGATAAAAATGTTTTAGc                                                                                                                                                                                                                                      <  1:50522/251‑1 (MQ=255)             aaCGTCACCATTTTTGACGGCAAGAATAACGTTGTTGACCACGCTGATGGTGATTACGCCGATTAACCGATGATCTACCAGGCGTTTCAGCCTCTGCCGCGGTTTGGCGATAGCTACACACTCATCGGTAGCTGGATTGTC‑GATGATGAAGCGTGCGGAATGGTGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACTTTTCGTTCCGCATTACATTGCTGGATAAAAATGTTTTAGCAAtct                                                                                                                                                                                                                                 <  1:158945/251‑1 (MQ=255)             aaCGTCACCATTTTTGACGGCAAGAATAACGTTGTTGACCACGCTGATGGTGATTACGCCGATGAACCGATGATCTACCAGGCGTTTCAACCTCTGCCGCGGTTTGGCGATAGCTACACACTCATCGGTAGCTGGATTGTC‑GATGATGAAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAACGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAAAATGTTTTAGCAAtct                                                                                                                                                                                                                                 >  2:78670/1‑251 (MQ=255)              aCGTCACCATTTTTGACGGCAAGAATAACGTTGTTGACCACGCTGATGGTGATTACGCCGATGAACCGATGATCTACCAGGCGTTTCAACCTCTGCCGCGGTTTGGCGATAGCTACACACTCATCGGTAGCTGGATTGTC‑GATGATGAAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAAAATGTTTTAGCAAtctc                                                                                                                                                                                                                                >  1:76554/1‑251 (MQ=255)               cGTCACCATTTTTGACGGAAAGAATAACGTTGTTGACCACGCTGATGGTGATTACGCCGATGAACCGATGATCTACCAGGCGTTTCAACCTCTGCCGCGGTTTGGCGATAGCTACACACTCATCGGTAGCTGGATTGTC‑GATGATGAAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGTATAAAAATGTTTTAGCAATctct                                                                                                                                                                                                                               <  2:172027/251‑1 (MQ=255)                 tCACCATTTTTGACGGCAAGAATAACGTTGTTGACCACGCTGATGGTGATTACGCCGATGAACCGATGATCTACCAGGCGTTTCAACCTCTGCCGCGGTTTGGCGATAGCTACACACTCATCGGTAGCTGGATTGTC‑GATGATGAAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAAAATGTTTTAGCAATCTCttt                                                                                                                                                                                                                             >  2:65002/1‑251 (MQ=255)                                  aaGAATAACGTTGTTGACCACGCTGATGGTGATTACGCCGATGAACCGATGATCTACCAGGCGTTTCAACCTCTGCCGCGGTTTGGCGATAGCTACACACTCATCGGTAGCTGGATTGTC‑GATGATGAAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAAAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATgg                                                                                                                                                                                                            >  1:165858/1‑251 (MQ=255)                                                             ggTGATTACGCCGCTGAACCGATGATCTACCAGGCGTTTCAACCTCTGCCGCGGTTTGGCGATAGCTACACACTCATCGGTAGCTGGATTGTC‑GCTGATGACGCGTGCGGAATGTGGATCCGTGAAGATACCACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAAAATGTTTTATCAATCTCTTTCTGTCATGATTCCATGGCAGTGACCATACTAATGGTGACTGcc                                                                                                                                                                                  >  2:258926/1‑250 (MQ=255)                                                                   tACGCCGATGAACCGATGATCTACCAGGCGTTTCAACCTCTGCCGCGGTTTGGCGATAGCTACACACTCATCGGTAGCTGGATTGTC‑GATGATGAAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAAAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATg                                                                                                                                                                           >  1:206663/1‑251 (MQ=255)                                                                                cGATGATCTACCAGTCGTTTCAACCTCTGCCGCGGTTTGGCGATAGCTACACACTCATCGGTAGCTGGATTGTC‑GATGATGAAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAAAATGTTTTAGCAATCTCTTTCTGTCATGAATCAATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGTAGAcacac                                                                                                                                                              <  2:71113/251‑2 (MQ=255)                                                                                         aCCAGTCGTTTCAGCCTCTGCCGCGGTTTGGCGATAGCTACACACTCATCGGTAGCTGGATTGTC‑GATTATGAAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTGCGTGCCGCATTACATTTCTGGATAAAAATGTTTTAGCAAGCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCAc                                                                                                                                                         <  1:250561/247‑1 (MQ=255)                                                                                              gCGTTTCAACCTCTGCCGCGGTTTGGCGATAGCTACTCACTCATCGGTAGCTGGATTGTC‑GATGATGAAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAAAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCAGTGATGGAGTGAGACACAGTGCACTGGCAAACt                                                                                                                                                <  2:165858/251‑1 (MQ=255)                                                                                                            gCCGCGGTTTGGCGATAGCTACACACTCATCGGTAGCTGGATTGTC‑GATGATGAAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAAAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTa                                                                                                                                   <  2:95911/250‑1 (MQ=255)                                                                                                            gCCGCGGTTTGGCGATAGCTACACACTCATCGGTAGCTGGATTGTC‑GATGATGAAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGAGCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAAAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGttttt                                                                                                                                    <  2:196602/249‑1 (MQ=255)                                                                                                                 ggTTTGGCGATAGCGACACACTCATCGGTAGCTGGATTGTC‑GATGATGAAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAAAATGTTTTAGCAATCTCGTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACGGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAAc                                                                                                                             <  2:121916/251‑1 (MQ=255)                                                                                                                                     cgcatcgtgaGCTGGAT‑GTCCGATGATGAAGCGTACGGAATGGGGATCCGTGAAGATAACACACGGAACACCAAAGACACCCCACGTTTCGTTCCGCATTTCATTGCTTGATAAAAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCATTCACCATACTAATGGGGACGGCCATTGTTGGAGGGAGAAACAGTGCAGTGGGAAACTCACTCCGGTTTTAATCAACCTATACCATTAAATAACAGc                                                                                                         <  2:142024/242‑1 (MQ=255)                                                                                                                                       cagcgtgatcgtgATTGTC‑GATGATGAAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAAAATGTTTTATCAATCTCTTTCGTTCATTAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGTAGACACAGTGCACTGGCAAACTAACACCGTTTTTAATCAACCTATACCATTAAATAACAGCac                                                                                                       <  2:149332/239‑2 (MQ=255)                                                                                                                                       cATCGGTAGCTGGATTGTC‑GATGATGAAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAAAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCaa                                                                                                       <  2:149327/251‑1 (MQ=255)                                                                                                                                          cGGTAGCTGGATTGTC‑GATGATGAAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCACAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAAAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCCTTAACTAACAGCAACtt                                                                                                    >  2:189638/1‑251 (MQ=255)                                                                                                                                          cGGTAGCTGGATTGTC‑GATGATGAAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAAAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTGTAATCAACCTATACCATTAAATAACAGCAACtt                                                                                                    <  2:55946/251‑1 (MQ=255)                                                                                                                                            gTAGCTGGATTGTC‑GATGATGAAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCTCGTTTCGTTCCGCATTACATTGCTGGATAAAAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTtat                                                                                                  <  2:105820/251‑1 (MQ=255)                                                                                                                                                           gatgatGAAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTACGCATTACATTGCTGGATAAAAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGgc                                                                                    >  1:21068/1‑251 (MQ=255)                                                                                                                                                            atgatgAAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAAAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGgcg                                                                                   >  1:81655/1‑251 (MQ=255)                                                                                                                                                                   aGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAAAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTg                                                                            <  2:202288/251‑1 (MQ=255)                                                                                                                                                                       tGCGGAATGGGGATCCGTGAAGATAACCCACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAAAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGAACTGGCAAACt                                                                                                                                                >  2:176037/1‑179 (MQ=255)                                                                                                                                                                       tGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAAAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACt                                                                                                                                                <  1:176037/179‑1 (MQ=255)                                                                                                                                                                               ggggATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAAAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAAc                                                                <  1:95886/251‑1 (MQ=255)                                                                                                                                                                                         gAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAAAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAgg                                                       <  1:225381/250‑1 (MQ=255)                                                                                                                                                                                          aaGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAAAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCTCTCTGCTAAGCGGTAACGCGTGAAGGTg                                                     <  2:260184/251‑1 (MQ=255)                                                                                                                                                                                                                       tCACGTTTCGTTCCGCATTACATTGCTGGATAAAAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGt                        >  1:143211/1‑251 (MQ=255)                                                                                                                                                                                                                         aCGTTTCGTTCCGCATTACATTGCTGGATAAAAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTAt                      <  1:99967/251‑1 (MQ=255)                                                                                                                                                                                                                           gTTTCGTTCCGCATTACATTGCTGGATAAAAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGCTTTTCTAGCCAGTATTg                    >  2:66434/1‑251 (MQ=255)                                                                                                                                                                                                                           gTTTCGTTCCGCATTACATTGCTGGATAAAAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTg                    >  2:80705/1‑251 (MQ=255)                                                                                                                                                                                                                                gTTCCGCCATACAGTGCTGGATAAAAATGTTTTAGCAATCTCTTTCTGTCATGAATACAGGGCAGTGACCATACTCATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCACACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGTTTTTCTAGACAGTATTggac                 >  2:34369/1‑247 (MQ=255)                                                                                                                                                                                                                                gTTCCGCATTACATTGCTGGATAAAAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTcag               <  2:222967/251‑1 (MQ=255)                                                                                                                                                                                                                                          aCATTGCTGGATAAACATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCCTTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGt                                                                   >  2:178854/1‑189 (MQ=255)                                                                                                                                                                                                                                          aCATTGCTGGATAAAAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGt                                                                   <  1:178854/189‑1 (MQ=255)                                                                                                                                                                                                                                          aCATTGCTGGATAAAAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGCTTTTCTAGCCAGTATTGGTCAGCAGTTAGGa      >  2:55943/1‑250 (MQ=255)                                                                                                                                                                                                                                              tGCTGGATAAAAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTGAGGAACgg  <  1:55943/250‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                CGCGAAGGCGGCAACGTCACCATTTTTGACGGCAAGAATAACGTTGTTGACCACGCTGATGGTGATTACGCCGATGAACCGATGATCTACCAGGCGTTTCAACCTCTGCCGCGGTTTGGCGATAGCTACACACTCATCGGTAGCTGGATTGTC‑GATGATGAAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGG  >  NC_000913/4413946‑4414431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑