Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,344,935	G→A	T208T (ACC→ACT)	yjdF ←	DUF2238 domain‑containing inner membrane protein YjdF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,344,935	0	G	A	100.0%	121.0 / NA	39	T208T (ACC→ACT)	yjdF	DUF2238 domain‑containing inner membrane protein YjdF
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/15);  total (24/15)

ATCCAGATCCATCTGCTGGATAAATATCGCAAAGTACCGCCCGAGCCTGTTCATGCTGATATTCCGGTCGGTGCAGTGAGTGATGTGAAAGCCTGACGTGGACAATAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCGGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAGCGCGCAGAACATATCGGATTGCGTGTCCCACTGGTCGCCCTGGGTGCCGAGAAAATCATCCGCTCCCTGACCCATTGCCAGCGCTGCCCACCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCG  >  NC_000913/4344695‑4345180                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      atccagatccaTCTGCTGGATAAATATCGCAAAGTACCGCCCGAGCCTGTTCATGCTGATATTCCGGTCGGTGCAGTGAGTGATGTGAAAGCCTGACGTGGACAATAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGc                                                                                                                                                                                                                                             >  1:106543/1‑251 (MQ=255)  tccagatccaTCTGCTGGATAAATATCGCAAAGTACCGCCCGAGCCTGTTCATGCTGATATTCCGGTCGGTGCAGTGAGTGATGTGAAAGCCTGACGTGGACAATAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGcc                                                                                                                                                                                                                                            >  2:216257/1‑251 (MQ=255)               gctgGATAAATATCGCAAAGTACCGCCCGAGCCTGTTCATGCTGATATTCCGGTCGGTGCAGTGAGTGATGTGAAAGCCTGACGTGGACAATAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGCTACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAg                                                                                                                                                                                                                                <  1:94666/250‑1 (MQ=255)                                     ccGCCCGAGCCTGTTCATGCTGATATTCCGGTCGGTGCAGTGAGTGATGTGAAAGCCTGACGTGGACAATAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAagcgagcg                                                                                                                                                                                                          >  2:76802/1‑250 (MQ=255)                                           gAGCCTGTTCATGCTGCTCTTCCGGTCGGTGCAGTGAGTGAAGTGAAAGCCTGACGTGGACAATAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAACTTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGAGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAat                                                                                                                                                                                                    >  2:177022/1‑250 (MQ=255)                                                      tGCTGATATTCCGGTCGGTGCAGTGAGTGATGTGAAAGCCTGACGTGGACAATAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCATTGAAAGCGAGCGAGGAATATCACCGTCGtt                                                                                                                                                                                        >  1:251074/1‑251 (MQ=255)                                                                 cggtcggtGCAGTGAGTGATGTGAAAGCCTGACGTGGACAATAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCa                                                                                                                                                                               >  2:256262/1‑249 (MQ=255)                                                                   gtcggtGCAGTGAGTGATGTGAAAGCCTGACGTGGACAATAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATAAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCACCGTAGCTGGAGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAagc                                                                                                                                                                            >  1:110505/1‑250 (MQ=255)                                                                     cggtGTAGTGAGTGATGTGAAAGCCTGACGTGGACAATAGCTAACGAAAAGGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCTTTAATGCGCCAagcag                                                                                                                                                                          <  1:65015/250‑1 (MQ=255)                                                                                   aTGTGAAAGCCTGACGTGGACAATAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGCCCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTAGCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAGCGCGCAGAACATATc                                                                                                                                                           <  2:19903/251‑1 (MQ=255)                                                                                   aTGTGAAAGCCTGACGTGGAAAATAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGCAGACCGGATAAGGAATTCACGCCGCGTCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTAGCAAGCCAATGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGGCGTTAATGCGCCAAGCAGCGCGCAGAACATATc                                                                                                                                                           <  2:251074/251‑1 (MQ=255)                                                                                      tgAAAGCCTGACGTGGACAATAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAGAGCGCAGAACATATCggc                                                                                                                                                        >  2:88268/1‑250 (MQ=255)                                                                                           gCCTGACGTGGACAATAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAGCGCGCAGAACATATCGGATTGCg                                                                                                                                                   >  2:233505/1‑251 (MQ=255)                                                                                                      aCAATAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGCAAGCGAGCGCGGAATATCACCGTCGTTAATGCGCCAAGCAGCGCGCAGCACATATCGGATTGCTTGTCCCACTgg                                                                                                                                        >  1:12395/1‑251 (MQ=255)                                                                                                        aaTAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAGCGCGCAGAACATATCGGATTGCGTGTCCCACTGGTc                                                                                                                                      >  2:268957/1‑251 (MQ=255)                                                                                                               aaCGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAGCGCGCAGAACATATCGGATTGCGTGTCCAACTGGTCGCCCTgg                                                                                                                               >  1:229260/1‑251 (MQ=255)                                                                                                                   aaaaTAGCATGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGTTAAGGAATTCACGCCGCATACGGCATCAACAAAGCGCAAGTTGTTATACAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAGCGCGCAGAACATATCGGCTTGCGTGTCCCACTGTTCGCCCTgggagc                                                                                                                           >  2:188403/1‑248 (MQ=255)                                                                                                                    aaaTGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAGCGCGCAGAACATATCGGATTGCGTGTCCCACTGGTCGCCCTGGGTGcc                                                                                                                          <  1:114902/251‑1 (MQ=255)                                                                                                                            tGATGCGCTATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAGCGCGCAGCACATATCGGATTGCGTGTCCCACTGGTCGCCCTGGGAGCCGAGaaact                                                                                                                  >  2:38584/1‑249 (MQ=255)                                                                                                                                       gTTTACCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAGCGCGCAGAACATATCGGATTGCGTGTCCCACTGGTCGCCCTGGGTGCCGAGAAAATCATCCGCTcc                                                                                                        <  1:233505/250‑1 (MQ=255)                                                                                                                                         ttATCAGGCCACGTCTTAATGAAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCTCCGTAGCTTGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCATCGCGCAGAACATATCGGATTGCGTGTCCCACTGGTCGCCCTGGGTGCCGAGAAAATCATCCGCTCCCt                                                                                                      <  1:267391/250‑1 (MQ=255)                                                                                                                                                             gtaatgCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAGCGCGCAGAACATATCGGATTGCGTGTCCCACTGGTCGCCCTGGGTGCCGAGAAAATCATCCGCTCCCTGACCCATTGCCAGCGCTGcc                                                                                  <  2:147022/250‑1 (MQ=255)                                                                                                                                                              taatgCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAGCGCGCAGAACATATCGGATTGCGTGTCCCACTGGTCGCCCTGGGTGCCGAGAAAATCATCCGCTCCCTGACCCATTGCCAGCGCTGcca                                                                                 >  1:134561/1‑249 (MQ=255)                                                                                                                                                               aatgCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAGCGCGCAGAACATAGCGGATTGCGTGTCCCACTGGTCGCCCTGGGTGCCGAGAAAATCATCCGCTCCCTGACCCATTGCCAGCGCTGCccac                                                                               <  2:239018/251‑1 (MQ=255)                                                                                                                                                                     ttGGATTTGCACATTTTTCAGACCGGATAAGCAATTCACGCCGCCTCAGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGACAAAGCGCCGTAGCTGGCGGCAATGAAAGAGAGCGCGGAATATCACCGTCGTTAATGCGCCACGCAGCGCGCAGCACATATCGGATTGCGTGTCCCACTGGTCGCCCTGGGTGCCGAGAACATCCTCCGCTCCCTGACCCATTCCCAGCGCTGCCAACCACTCg                                                                         >  2:98233/1‑251 (MQ=255)                                                                                                                                                                        gATTTGCAACTTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGACGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAGCGCGCAGAACATATCGGATTGCGTGTCCCACTGGTCGCCCTGGGTGCCGAGAAAATCATCAGCTCCCTGACCCATTGCCAGCGCTTCCCACCACCCGAtt                                                                      >  2:61821/1‑251 (MQ=255)                                                                                                                                                                                            cGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAGCGCGCAGAACATATCGGATTGCGTGTCCCACTGGTCGCCCTGGGTGCCGAGAAAATCATCCGCTCCCTGACCCATTGCCAGCGCTGCCCACCACTCGATTAATTCAGACATCGCGCTTAt                                                  <  1:216257/251‑1 (MQ=255)                                                                                                                                                                                             ggATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAGCGCGCAGAACATATCGGATTGCGTGTCCCACTGGTCGCCCTGGGTGCCGAGAAAATCATCCGCTCCCTGACCCATTGCCAGCGCTGCCCACCACTCGATTAATTCATACATCGCGCTTATc                                                 >  1:253534/1‑251 (MQ=255)                                                                                                                                                                                                tAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCAGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAGCGCGCAGAACATATCGGATTGCGTGTCCCACTGGTCGCCCTGGGTGCCGAGAAAATCATCCGCTCCCTGACCCATTGCCAGCGCTGCCCACCACTCGATTAATTCATACATCGCGCTTATCGcc                                              >  1:267157/1‑251 (MQ=255)                                                                                                                                                                                                        tCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAGCGCGCAGAACATAGCGGATTGCGTGTCCCACTGGTCGCCCTGGGTGCCGAGAAAATCATCCGCTCCCTGACCCATTGCCAGCGCTGCCCACCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCa                                       <  2:253534/250‑1 (MQ=255)                                                                                                                                                                                                        tCACGCCGCATCCGGCATCAACAAAGCGCAAGTGGTTATCCAGGTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAGCGCGCAGAACATATCGGATTGCGTGTCCCACTGGTCGCCCTGGGTGCCGAGAAAATCATCCGCTCCCTGACCCATTGCCAGCGCTGCCCACCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCa                                       <  2:96011/250‑1 (MQ=255)                                                                                                                                                                                                              cgcATCCGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAGCGCGCAGAACATATCGGATTGCGTGTCCCACTGGTCGCCCTGGGTGCCGAGAAAATCATCCGCTCCCTGACCCATTGCCAGCGCTGCCCACCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACgcagc                                 >  2:238169/1‑250 (MQ=255)                                                                                                                                                                                                               gcATCCGGCATCAACAAAGCGCAAGTTGTTAACCAGTGATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGGTAATGCGCCAAGCAGCGCGCAGAACATATCGGATTGCGTGTCCCACTTGTCGCCCTGGGTGCCGAGAAAATCATCCGCTCCCTGACCCATTGCCAGCGCTGCCCACCACTCGATTAATGCATACATCGCGCTTATCGCCAGCGCCACGcagcag                               <  1:98233/251‑1 (MQ=255)                                                                                                                                                                                                                    cGGCATCAACAAAGCGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAGCGCGCAGAACATATCGGATTGCGTGTCCCACTGGTCGCCCTGGGTGCCGAGACAATCATCCGCTCCCTGACCCATTGCCAGCGCTGCCCACCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGAcc                            >  1:93309/1‑249 (MQ=255)                                                                                                                                                                                                                      gCATCAACAAAGAGCAAGTTGTTATCCAGTTATCAAGCCAAAGCGCCGGAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAGCGCGCAGAACATATCGGATTGCGTGTCCCACTGGTCGCCCTGGGTGCCGAGAAAATCATCCGCTCCCTGACCCATTGCCAGCGCTGCCCACCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGa                        <  2:106543/251‑1 (MQ=255)                                                                                                                                                                                                                            acaaAACGCAAGTTGTTATCCAGTTATCCAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGCGGAATATCACCGTCGTTAATGCGCCAAGCAGCGCGCAGAACATATCGGATTGCGTGTCCCACTGGTCGACCTGGGTGCCGAGAAAATCATCCGCTCCCTGACCCATTGCCAGCGCTGCCCACCACTCGATTACTTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGCCCAGGAACGcc                   >  2:266003/1‑250 (MQ=255)                                                                                                                                                                                                                                         tgttACCCAGTTAGCAAGCCAAAGAGCAGCAGCTGGAGGCAATGACAGCGAGCGAGGAATACCCTCGACGTTAATGCGCCAAGCAGCGCGCAGTACATATCGGATTGCGTGTCACACTGGTCACCCTGGGTGACGAGAAAATCATACGCTCCCTGACCCATTGCCAGCGCTGCCCACCAATCGCTTAATTCATACATAGCGCTTATCGCCAGCGCAACGCAGCAGACCAGGAACGCCACCATTTTACGTcc     >  2:268377/1‑251 (MQ=255)                                                                                                                                                                                                                                           ttATCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGATCGAGGAATATCACCGTCGTTAATGCGCCACGCAGCGCGCAGAACATATCGTATTGCGTGTCCCACTGGTCGCCCTGGGTGCCGAGAAACTCATCCGCTCCCTGACCCATTGCCAGCGCTGCCCACCACTCGATTAATTCCTCCATCGCGCTTATCGCCCGCGCCACGCAGCAGACCAGGACCGCCACCATTTTACGTctgc   >  2:107055/1‑248 (MQ=255)                                                                                                                                                                                                                                             aTCCAGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAGCGCGCAGAACATATCGGATTGCGTGTCCCACTGGTCGCCCTGGGTGCCGAGAAAATCATCCGCTCCCTGACCCATTGCCAGCGCTGCCCACCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCcgcg  <  1:121860/250‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      ATCCAGATCCATCTGCTGGATAAATATCGCAAAGTACCGCCCGAGCCTGTTCATGCTGATATTCCGGTCGGTGCAGTGAGTGATGTGAAAGCCTGACGTGGACAATAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCGGTTATCAAGCCAAAGCGCCGTAGCTGGCGGCAATGAAAGCGAGCGAGGAATATCACCGTCGTTAATGCGCCAAGCAGCGCGCAGAACATATCGGATTGCGTGTCCCACTGGTCGCCCTGGGTGCCGAGAAAATCATCCGCTCCCTGACCCATTGCCAGCGCTGCCCACCACTCGATTAATTCATACATCGCGCTTATCGCCAGCGCCACGCAGCAGACCAGGAACGCCACCATTTTACGTCCGCG  >  NC_000913/4344695‑4345180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑