Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,340,953	C→T	G226R (GGG→AGG)	melR ←	DNA‑binding transcriptional dual regulator MelR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,340,953	0	C	T	100.0%	61.6 / NA	22	G226R (GGG→AGG)	melR	DNA‑binding transcriptional dual regulator MelR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/12);  total (10/12)

AGCCGGGAAACGTCTGGCGGCGCTGTTGGCTAAGTTTGCGGTATTGTTGCGGCGACATGCCGACATATTTGCCGAACGTGCTGTAAAAACGACTACTTGAACGAAAGCCTGCCGTCAGGGCAATATCGAGAATACTTTTATCGGTATCGCTCAGTAACGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATCGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTC  >  NC_000913/4340722‑4341190                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                              aGCCGGGAAACGTCTGGCGGCGCTGTTGGCTAAGTTTGCGGTATTGTTGCGGCGACATGCCGACATATTTGCCGAACGTGCTGTAAAAACGACTACTTGAACGAAAGCCTGCCGTCAGGGCAATATCGAGAATACTTTTATCGGTATCGCTCAGTAACGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGtt                                                                                                                                                                                                                             <  2:225196/250‑1 (MQ=255)             tCTGGCGGCGCTGTTGGCTAAGTTTGCGGTATTGTTGCGGCGACATGCCGACATATTTGCCGAACGTGCTGTAAAAACGACTACTTGAACGAAAGCCTGCCGTCAGGGCAATATCGAGAATACTTTTATCGGTATCGCTCAGTAACGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGc                                                                                                                                                                                                                >  1:72334/1‑251 (MQ=255)                       cggTTGGCTAAGTTTGCGGTATTGTTGCGGCGACATGCCGACATATTTGCCGAACGTGCTGTAAAAACGACTACTTGAACGAAAGCCTGCCGTCAGGGCAATATCGAGAATACTTTTATCGGTATCGCTCAGTAACGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACAt                                                                                                                                                                                                      <  2:216255/249‑1 (MQ=255)                                        ggTATTTTTGCGTCGACATGCCGACTTATTTGCCGAACGAGCTGTAAAAACGACTACTTGAACGAAATCCTGCCGTCAGGGCAATATCGAGAATACTTTTAGCGGTATCGCTCAGTATCTCTCGAACTTGGTTGATGCGCATCGCGGTAATTTACCGTTTCAGCGTCAATTGCATTACCCTCTGTAATATCCTCATTGCATAGTAGGCGTCACGTGTTACGTGCGCAGCCCCATCTTTTATGTTCAGCGcc                                                                                                                                                                                     <  2:243408/251‑1 (MQ=255)                                           aTTGTTGCGGCGACATGCCGACATATTTGCCGAACGTGCTGTAAAAACGACTACTTGAACGAAAGCCTGCCGTCAGGGCAATATCGAGAATACTTTTATCGGTATCGCTCAGTAACGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCt                                                                                                                                                                                                               >  2:167056/1‑222 (MQ=255)                                           aTTGTTGCGGCGACATGCCGACATATTTGCCGAACGTGCTGTAAAAACGACTACTTGAACGAAAGCCTGCCGTCAGGGCAATATCGAGAATACTTTTATCGGTATCGCTCAGTAACGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCt                                                                                                                                                                                                               <  1:167056/222‑1 (MQ=255)                                                 gcggcgACATGCCGACATATTTGCCGAACGTGCTGTAAAAACGACTACTTGAACGAAAGCCTGCCGTCAGGGCAATATCGAGAATACTTTTATCGGTATCGCTCAGTACCGCGTGAACGTGGTTGATGCGCATCGCGGTAATGTACTTTTTCAGCGTCAATTGCATGACCCGCTGGAATATTCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATa                                                                                                                                                                             <  1:46910/250‑1 (MQ=255)                                                                              gTGCTTTAAAAACGACTACTTGAACGAAAGCCTGCCGTCAGGGCAATATCGAGAATACTTTTATCGGTATCGCTCAGTAACGCGCGAACGTGGTTGATGCGCATCGCGGTAATTTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGCTGGCGTTAAGTTTTACGTTCTCAGCCCCATCGTTGATGGTCAGCGCCTGAGCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCt                                                                                                                                               <  2:247778/251‑1 (MQ=255)                                                                                   gTAAAAACGACTACTTGAACGAAAGCCTGCCGTCAGGGCAATATCGAGAATACTTGTATCGGTATCGCTCAGTAACGCGCGAACGTTGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGCCCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACAt                                                                                                                                          <  1:138237/251‑1 (MQ=255)                                                                                     aaaaaCGACTACTTGAACGAAAGCCTGCCGTCAGGGCAATATCGAGAATACTTTTATCGGTATCGCTCAGTAACGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGGCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATaa                                                                                                                                        <  1:228272/251‑1 (MQ=255)                                                                                         aCGACTACTTGAACGAAAGCCTGCCGTCAGGGCAATATCGAGAATACTTTTATCGGTATCGCTCAGTAACGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCAACATAGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAACGCCCAGCATCTGGCTAACATaacaat                                                                                                                                    >  2:228059/1‑247 (MQ=255)                                                                                                                ccgtcagaaaaaTACCGAGAATACTTTTATCGGTATCGCTCAGTAACGCGCGAACGTGGTTGATGCGCCTCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGTTGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGACCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGttt                                                                                                             >  1:201318/11‑251 (MQ=255)                                                                                                                            tatCGAGAATACTTTTATCGGTATCGCTCAGTAACGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGttttt                                                                                                           <  1:206921/241‑1 (MQ=255)                                                                                                                            tatCGAGAATACTTTTATCGGTATCGCTCAGTAACGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGttttt                                                                                                           >  2:206921/1‑241 (MQ=255)                                                                                                                             atCGAGAATACTTTTATCGGTATCGCTCAGTAACGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGcgcg                                                                                                >  1:14715/1‑251 (MQ=255)                                                                                                                                 agaATACTTTTATCGGTATCGCTCAGTAACGCGCGAACGTGGTTGCCGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATACACTCATTGCATAGTTGGCGTTAAGTTTGCCGTGCTCAGCCACATCGTTGATGGTCAGCGACTGATCATAGTTTTCGGCAATAAAGCCCAGCCTCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGt                                                                                            >  1:124104/1‑251 (MQ=255)                                                                                                                                               ggTATCGCTCAGTAACGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGa                                                                               >  2:212319/1‑250 (MQ=255)                                                                                                                                                         aGTAACGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCcca                                                                    <  1:81533/251‑1 (MQ=255)                                                                                                                                                                     aCGTGGGTGATGCGCATCGCGGTAATGTTCTGTTTCAGCGTCAATTGCATGACCCGCTGGAAGATCCTCATTGCATAGTGGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCt                                                        <  1:138285/251‑1 (MQ=255)                                                                                                                                                                                       gctgTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTGTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATCGCTTGAGCATCAg                                      <  2:72334/248‑1 (MQ=255)                                                                                                                                                                                          gTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATCGCTTGAGCATCAGGcc                                   >  1:98929/1‑251 (MQ=255)                                                                                                                                                                                                                           cGCTGGAATATCCTCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGAAACATCGTTGATGGTCAGCGCCTGATCATAGATTTAGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGAGCATGGAGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATCGCTTGAGCATCAGGCCAATTTCATCAATGACGACCTGGCGCATTTGCTc  >  1:244519/1‑251 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                              AGCCGGGAAACGTCTGGCGGCGCTGTTGGCTAAGTTTGCGGTATTGTTGCGGCGACATGCCGACATATTTGCCGAACGTGCTGTAAAAACGACTACTTGAACGAAAGCCTGCCGTCAGGGCAATATCGAGAATACTTTTATCGGTATCGCTCAGTAACGCGCGAACGTGGTTGATGCGCATCGCGGTAATGTACTGTTTCATCGTCAATTGCATGACCCGCTGGAATATCCCCATTGCATAGTTGGCGTTAAGTTTGACGTGCTCAGCCACATCGTTGATGGTCAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATCGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTC  >  NC_000913/4340722‑4341190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑