Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,331,890	C→T	L463L (CTC→CTT)	proP →	osmolyte:H(+) symporter ProP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,331,890	0	C	T	100.0%	61.1 / NA	23	L463L (CTC→CTT)	proP	osmolyte:H(+) symporter ProP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/16);  total (7/16)

CGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTACCTGATGGTAGTGGCGGTGGTTGGTTTAATCACCGGCGTAACCATGAAAGAGACGGCAAATCGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGATATAC‑AGGAAGCGAAGGAAATTCTCGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATCTCTGACGCGCATACTCTCCTCCAGGTTAACGGAGGAGAGTGCAATGAAAAACCGTGTTTATGA  >  NC_000913/4331643‑4332135                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      cgggggatgtCCGTTACAGCTCGCGGACGGCGGCATCTAGTATTGCGGTGCTGGTTGCCGGTCTGACGCCGACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTACCTGATGGTAGTGGCGGTGTTTGGTTTAATCACCGGCGTAACCAGGAAAGATACGGCAAATCGGGCGTTGAAAGGAGCGACACCGGCGTGGACAGATGTAC‑AGGACGCGAAGGAAATTCttgtc                                                                                                                                                                                                                                                    <  2:31009/242‑1 (MQ=255)                           gcggcggcATTTAATATGTCGGTGCTGGTGGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTACCTGATGGTAGTGGCGGTGGTTGGTTTAATCACCGGCGTAACCATGAAAGAGACGGCAAATCGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGATATAC‑AGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGATCAGaa                                                                                                                                                                                                                          <  1:175982/251‑1 (MQ=255)                                  cATTTAATAGTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTACCTGATGGTAGTGGCGGTGGTTGGTTTAATCACCGGCGTAACCATGAAAGAGACGGCAAATCGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGATATAC‑AGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCgat                                                                                                                                                                                                                   <  2:200875/251‑1 (MQ=255)                                                                 gACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTACCTGATGGTAGTGGCGGTGGTTGGTTTAATCACCGGCGTAACCATGAAAGAGACGGCAAATCGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGATATAC‑AGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGc                                                                                                                                                                                       <  1:195223/248‑1 (MQ=255)                                                                  gcGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTACCTGATGGTAGTGGCGGTGGTTGGTTTAATCACCGGCGTAACCATGAAAGAGACGGCAAATCGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGATATAC‑AGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGc                                                                                                                                                                                   <  2:78558/250‑1 (MQ=255)                                                                                            cGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTACCTGATGGTAGTGGCGGTGGTTGGTTAAATCACCGGCGTAACCATGAAAGAGACGGAAAATCGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGATAGAC‑AGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAAGCGATGATATTGACCACGAGATTGCCGATTGGCGGGCGAAACGTCCTCGCCTGGTGCAGCAAc                                                                                                                                                         <  2:164061/251‑1 (MQ=255)                                                                                              aaaGCTGGCAGAATCTGATGATGCCTGCGTATTACCTGATGGTAGGGGCGGTGGTTGGTTTAATCACCGGCGTAACCATGAAAGAGACGGAAAAACGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGATATAC‑AGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGGGAAACGTCCCCGCCTGGTGCAGCAACAt                                                                                                                                                       <  2:24224/251‑1 (MQ=255)                                                                                                              gatgatTCCTGCTTATTACCTGAGGGTAGTGGTGGTGGTTGTTTTAATCACCGGCTTAACCATGAAGGAGACGGAAAATGGTCCGTTAAAAGGTGCGAAACCGGCGGGGTAAAAT‑TACCAGAAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATACCGACCAGAAAACCGAGGATAGTGACCACAAGATGGCGGAGTTGCAGGTGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGaa                                                                                                                                        <  2:181458/250‑1 (MQ=255)                                                                                                              gatgatGCCTGCCTATTACCGGATGGTAGTGGCGGTGTTTGGTTTAATCACCGGCGTAACCATGAAAGAGACGGCAAATCGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGATATAC‑AGGAAGCGAAGGAAATTCTTGTCGATCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTTCCGATTTGCAGGCAAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATtgat                                                                                                                                           <  2:123842/247‑1 (MQ=255)                                                                                                                                  ggATGGTATGGGCGGTGGTTGGTTTAATCCCCGGCGTAACCATGAAAGAGACGGCAAATCGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGATATAC‑AGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTg                                                                                                                   <  2:240561/250‑1 (MQ=255)                                                                                                                                                       gttTAATCACCGGCGTAACCATGAACGAGACGGCAACTCGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGATATAC‑AGGCAGCGACGGAAATTCTTGTCGAGCCTTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAgg                                                                                              >  2:134232/1‑251 (MQ=255)                                                                                                                                                       gttTAATCACCGGCGTAACCATGAAAGAGAGGGAAAATCGTCCGTTGAAAGGTGCGACCCCGGCGGCGTCAGATATAC‑AGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAgg                                                                                              <  1:126057/251‑1 (MQ=255)                                                                                                                                                           aaTCACCGGCGTAACCATGAAAGAGACGGCAAATCGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGATATAC‑AGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCa                                                                                           >  1:68984/1‑250 (MQ=255)                                                                                                                                                              cACCGGCGTAACCATGAAAGCGACGGCAAATCGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGATATAC‑AGGAAGCGAAGGAAATTCTTGTCGCGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGCTGGCCTGCTGTGACGCTGTCTTATCAGGCCAATTg                                                                                       >  2:20612/1‑251 (MQ=255)                                                                                                                                                                 cgtcGTAACCATGAAAGAGACGGCAAATCGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGATATAC‑AGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCCCGAGATTGCCGATTTGCAGGCGAAACGTCCCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAAc                                                                                    <  1:237/248‑1 (MQ=255)                                                                                                                                                                    ggTAACCATGAAAGAGACGGCAATTCGTCCGTTGAAAGGGGCGACACCGGCGGCGGCAGATATAC‑AGGAAGCGAAGGAAATTTTTGTCGAGCATTACGATAATATCGAGCAGAAAAGCGATGATATTGACCACGGGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAActct                                                                                 <  1:262493/250‑1 (MQ=255)                                                                                                                                                                                                     gAAAGGTGCGACACCGGCGGCGTCAGATATAC‑AGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATCTCTGACGCGCATActct                                                <  1:67378/251‑1 (MQ=255)                                                                                                                                                                                                       aaGGTGCGACACCGGCGGCGTCAGATATAC‑AGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATCTCTGACGCGCATACTctcc                                              <  1:185714/251‑1 (MQ=255)                                                                                                                                                                                                        aGGTGCGACACCGGCGGCGTCAGATATAC‑AGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCCGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACCCTGTCTTATCAGGCCAATTGAAATCTTAAGGTTCACTTAATCTCTGACGCGCATACTctccc                                             >  2:92088/1‑250 (MQ=255)                                                                                                                                                                                                             cGACACCGGCGGCGTCAGATATAC‑AGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATCTCTGACGCGCATACTCTCCTCCAgg                                        <  1:55048/251‑1 (MQ=255)                                                                                                                                                                                                                  ccGGCGGCGTCAGATATAC‑AGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATCTCTGACGCGCATACTCTCCTCCAGGTTacc                                   >  1:175779/1‑249 (MQ=255)                                                                                                                                                                                                                   cggcggCGTCAGATATAC‑AGGCAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAAGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGCTGAATAAGCTGAAACGGATGGCCTGATGTGCAGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATCTCTGACGCGCATACTCTCCTCCAGGTTAACg                                  >  2:239833/1‑251 (MQ=255)                                                                                                                                                                                                                                   aC‑AGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATCTCTGACGCGCATACTCTCCTCCAGCTTAACGGAGGAGAGTGCAATg                   >  1:160415/1‑250 (MQ=255)                                                                                                                                                                                                                                                    aTTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGTGTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTGAAGGTTCACTTAATCTCTAACGCGCATACTCTCCTCCAGGTTAACGGAGGAGAGTGCAATGAAAAACCGTGTTTATGa  <  1:35827/251‑1 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      CGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTACCTGATGGTAGTGGCGGTGGTTGGTTTAATCACCGGCGTAACCATGAAAGAGACGGCAAATCGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGATATAC‑AGGAAGCGAAGGAAATTCTCGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATCTCTGACGCGCATACTCTCCTCCAGGTTAACGGAGGAGAGTGCAATGAAAAACCGTGTTTATGA  >  NC_000913/4331643‑4332135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑