Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,322,786	G→A	intergenic (‑364/+550)	phnF ← / ← phnD	putative transcriptional regulator PhnF/phosphonate/phosphate ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,322,786	0	G	A	97.7%	128.4 / ‑6.7	45	intergenic (‑364/+550)	phnF/phnD	putative transcriptional regulator PhnF/phosphonate/phosphate ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (16/27);  minor base C (1/0);  total (17/28)
Fisher's exact test for biased strand distribution p-value = 3.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.80e-01

AAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTCGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCC‑GGTGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGGGCCGAGGCCGACGGCGACCACGAACAGCATGGCGAAGACCATTTCGTTAATGGCGCGGCAGGCGTCCATCAGGCGGCGAACGGGCTGGTAAACCCACCACGGCACCAGGTTTTCGGCGCTCATCAGGCCAAAGGGGATGGAGAGAACCACCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGC  >  NC_000913/4322540‑4323015                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                      aatcctcgAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTCGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCATTCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCC‑gatggt                                                                                                                                                                                                                                   <  1:184228/245‑1 (MQ=255) aaaCCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTCGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCC‑gatggg                                                                                                                                                                                                                                   >  1:16301/1‑250 (MQ=255)        gaacgtctccccACAGGGGGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTCGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCGCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCC‑GATGGTGTGGATa                                                                                                                                                                                                                            <  2:266302/242‑1 (MQ=255)               ttCCCACAGAGTGACGCCGATCCCGCCTGCGCCGCCCATGACGACGCCGGTCGCAGAGCGGACGTTGGATTCGAAGCGATCGAGGGAGTCAGAGATCCGCAGTGGCATCCCCTGAGGCAGCACGCCGTAGAGGCTCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCCATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCC‑GATGGTGAGGATAAACAgcg                                                                                                                                                                                                                     >  2:130839/1‑251 (MQ=255)                  ccACAGGGTGACGCCGATCACGCCTGCGCCCACCATGACGACGCCGGTCGCCGAGCGGACGTTGGATTCGACGCGATAGAGGGAGTCGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGCGCCTGTTGGCACCGGGGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACAGCTTCGGAAAGCAGCTTGGAGAGCCCGCC‑GCTGGTGTGGATAAACAGcgcc                                                                                                                                                                                                                   >  2:247336/1‑250 (MQ=255)                      aGGGTAACGCCGATCCAGCCTGCGCCGACCATGCCGACGACGGTCGCCGAGCGGACGTTGGATTCGACGCGATAGAGGGCGTAGGCGATCAGCAGTGGCCTCACCTGTGGCAGCACGCCGTCGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGCGCACGCC‑GATGGCGTGGAGAAACAGcgccagcac                                                                                                                                                                                                              >  2:134256/1‑251 (MQ=255)                       gggggACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTCGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCC‑GATGGTGTGGATAAACAGcgccagcacg                                                                                                                                                                                                             <  2:84398/247‑1 (MQ=255)                              gCCGATCCCGCCTGCGCCGACCATGCCGCCGCTGGTCGCCGAGCGGACGTTGGATTCGAAGCGATAGCGGGAGTAGGAGATCAGCAGTGGCATCCCCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGACTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCC‑GATGGTGTGGATAAACAGcgcacgcacgcaagccc                                                                                                                                                                                                      >  2:252258/1‑249 (MQ=255)                              gCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTCGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCC‑GATGGTGTGGATAAACAGCgccagcacgccagcac                                                                                                                                                                                                      >  1:166551/1‑251 (MQ=255)                                 gATCCCGCCTGCGCCGACCATGCCGACGACGGTCGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCC‑GATGGTGTGGATAAACAGCGCCagcacgccagcacgcc                                                                                                                                                                                                   >  1:113502/1‑251 (MQ=255)                                           gcgcCGACCATGCCGACGACGGTCGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCC‑GATGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACg                                                                                                                                                                                           <  1:162900/249‑1 (MQ=255)                                            cgcCGACCATGCCGACGACGGTCGCCGAGCGGGCGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCATCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCGCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGTAGAGCACGCC‑GATGGGGTGGATAAACAGCGCCAGCACGTCAGCACGCCAGCGAACGggc                                                                                                                                                                                        <  2:263281/251‑1 (MQ=255)                                                gACCATGCCGGCGACGGTCGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGGGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCC‑GATGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGggccgag                                                                                                                                                                                    <  2:62203/251‑1 (MQ=255)                                                gACCATGCCGACGACGGTCGCCGTGCGGACGTTGGATTCGAAGCGGTAGAGGGAGGAGGCGATCCGCAGCGGCATCACCTGGGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGACTGCCTTGCACCGGGCAAGGCTCCATCGCTGCCACCGCTACGGAAACCAGCTTGGAGAGCGCGCC‑GATGTCGCGGCCAAACAGCGCCAGCACGCCAGCACCCCCGCGAACGggccg                                                                                                                                                                                      >  2:74416/1‑249 (MQ=255)                                                            ggcgGTCGCAGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCAGCACCTGTGGCAGCCCGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCCCCGGGCCGGGCTCAATCGCTTCCACCACTTCGGAAAGCAGCTTGGAGAGCACGCC‑GATGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGGGCCGAGGCCGACGGCGAc                                                                                                                                                                        <  2:148499/249‑1 (MQ=255)                                                                   gCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGTGCTCAATCGCTTCCTCCGCTTCGGAAAGCAGGTTGTAGAGCACGCC‑GAGGGTGTGGATAAACAGCGCCAGCACGCCTGCACGCCAGCGAACGGGCCGAGGCCGACGGCGACCACGAAc                                                                                                                                                                 <  1:247336/251‑1 (MQ=255)                                                                                  gaatgtaggcgatgtagGGAGTAGGAGGTCAGCCGGGGCAGCAGCTGTGGCAGCCCGCCGTAGTGGATCTCTAAGAGCTTGTTGGCACCGGTGGCGCAAACGCCTGCCCCCGGGCCGGGGCCAATCGCTTCCACCGCGTAGGAAAGCAGCAGCGAGAGCACGCC‑GATGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGTGCCGAGGCGGACGGCGACCACGAACAGCTTGGCGGAGAcc                                                                                                                                                  <  2:17056/236‑1 (MQ=255)                                                                                  gATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGGGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCC‑GATGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGGGCCGAGGCCGACGGCGACCACGAACAGCATGGCGAAGAcc                                                                                                                                                  <  2:65226/251‑1 (MQ=255)                                                                                     tctaatcGATAGAGGGAGTAGGAGATCAGCAGTGGCATCCCCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCC‑GATGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGGGCCGAGGCCGACGGCGTCCACTAACAGCATGGCGAAGACCAt                                                                                                                                                <  2:254978/244‑1 (MQ=255)                                                                                         aGCGATAGAGGGAGTAGGAGAGCAGCAGGGGCATCTCCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCCCCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCGTGGAGAGCACGCC‑GATGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGGGCCGAGGCCGACGGCGACCACGAACAGCATGGCGAAGACCATTTCGt                                                                                                                                           <  2:149439/251‑1 (MQ=255)                                                                                            gATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCC‑GATGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGGGCCGAGGCCGACGGCGACCACGAACAGCATGGCGAAGACCATTTCGTTaa                                                                                                                                        <  2:220568/251‑1 (MQ=255)                                                                                            gATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCC‑GATGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGGGCCGAGGCCGACGGCGACCACGAACAGCATGGCGAAGACCATTTCGTTaa                                                                                                                                        <  1:92140/251‑1 (MQ=255)                                                                                                   ggAGTAGGAGATCAGCAGAGGCATCACCTGTGACAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCAGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCC‑GATGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGGGCCGAGGCCGACGGCGACCACGAACAGCATGGCGAAGACCATTTCGTTAATGGcgcg                                                                                                                                 >  1:236539/1‑251 (MQ=255)                                                                                                      gTAGGAGATCAGCAGTGGCAGCACCTGTGGCAGCTCGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCC‑GATGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGGGCCGAGGCCGACGGCGACCACGAACAGCATGGCGAAGACCATGTCGTGAATGGCGCGGCa                                                                                                                              <  1:85634/251‑1 (MQ=255)                                                                                                        aGGAGATCGGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCC‑GATGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCATCGAACGGGCCGAGGCCGACGGCGACCACGAACAGCATGGCGAAGACCATTTCGTTAATGGCGCGGCAg                                                                                                                             <  2:236742/250‑1 (MQ=255)                                                                                                               cagcagTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCC‑GATGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGGGCCGAGGCCGACGGCGACCACGAACAGCATGGCGAAGACCATTTCGTTAATGGCGCGGCAGGCGTCCa                                                                                                                      <  1:157120/250‑1 (MQ=255)                                                                                                                atcagTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCC‑GATGGTGTGGATAAACAGCGCCGGCACGCAAGCACGCCAGCGAACGGGCCGAGGCCGACGGCGAGCACGAACAGCATGGCGAAGACCATTTCGGTAAAGGCGCGCCAGGCGTcccac                                                                                                                    >  2:94893/3‑248 (MQ=255)                                                                                                                  cagTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCC‑GATGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGGGCCGAGGCCGACGGCGACCACGAACAGCATGGCGAAGACCATTTCGTTAATGGCGCGGCAGGCGTCCATCAg                                                                                                                  <  1:144311/251‑1 (MQ=255)                                                                                                                                            cGTAGAAGATCTCTACGAGCTTGATCGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATAGCTTCCACCGCTTCGGTATGCAGCTTGGCGAGCCCGCC‑GATGGTGTGGATCAACAGCGCCAGCACGCCAGCACGCCACCGAACGGGCCGCGGCCGCTGGCGACCACCAACAGCATGGCGAAGACCATTTCGTTAATGGCGCGGCAGGCGTACTTACGGCGGCGTAAGGGCTGGTTaaccaacc                                                                                        >  2:241530/1‑251 (MQ=255)                                                                                                                                             gTAGAGGATCTCTTCGCGCTTGTTGGCACCGGTGGCGCGCGTGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCC‑GATGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGGGCCGAGCCCGACGGCGACCACGAACAGCATCGCGAAGACCATTTCGTTAATGGCGCGGCAGGCGTCCATCAGGCGGCGCACGGGCTGGTAAACccacc                                                                                        >  2:165150/1‑250 (MQ=255)                                                                                                                                                gagGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCC‑GATGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGGGCCGAGGCCGACGGCGACCACGAACAGCATGGCGAAGACCATTTCGTTAATGGCGCGGCAGGCGTCCATCAGGCGGCGAACGGGCTGGGAACCCCACCACgg                                                                                    >  2:188731/1‑251 (MQ=255)                                                                                                                                                            gAGCTTGGTGGCGCCGGGGGCGCGAATGCCTTCCGCCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCC‑GATGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGGGCCGAGGCCGACGGCGACCACGAACAGCATGGCGAAGACCATTTCGTTAATGGCGCGGCAGGCGTCCATCAGGCGGCGAACGGGCTGGTAAACCCACCACGGCACCAGGTTTTc                                                                        <  2:217066/251‑1 (MQ=255)                                                                                                                                                                    tgGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCC‑GATGGTGTGGATAAACAACGCCAGCACGCCAGCACGCCAGCGAACGGGCCGAGGCCGACGGCGACCACGAACAGCATGGCGAAGACCATTTCGTTAATGGCGCGGCAGGCGTCCATCAGGCGGCGAACGGGCTGGTAAACCCACCACGGCACCAGGTTTTCGGCGCtcc                                                                >  1:252436/1‑250 (MQ=255)                                                                                                                                                                                      tGCCTTCCCCCGGGCCGGGCTCAATCGCTTCCCCCGCTTCGGAAAGCAGCTTGGAGAGCACGCC‑GATGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGGGCCGAGGCCGACGGCGACCACGAACAGCATGGCGAAGACCATTTCGTTAATGGCGCGGCAGGCGTCCATCAGGCGGCGAACGGGCTGGTAAACCcaccac                                                                                      <  2:2730/211‑1 (MQ=255)                                                                                                                                                                                      tGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCC‑GATGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGGGCCGAGGCCGACGGCGACCACGAACAGCATGGCGAAGACCATTTCGTTAATGGCGCGGCAGGCGTCCATCAGGCGGCGAACGGGCTGGTAAACCcaccac                                                                                      >  1:2730/1‑211 (MQ=255)                                                                                                                                                                                      ggCCTTCCACCGGGCCGGGCCCAAGCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCC‑GATGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGGGCCGAGGCCGACGGCGACCACGAACAGCATGGCGAAGACCATTTCGTTAATGGCGCGGCAGGCGTCCATCAGGCGGCGAACGGGCTGGTAAACCCACCACGGCACCAGGTTTTCGGCGCTCATCAGGCCAAAGGGGATgg                                              <  2:121606/250‑1 (MQ=255)                                                                                                                                                                                         cTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCC‑GATGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGGGCCGAGGCCGACGGCGACCACGAACAGCATGGCGAAGACCATTTCGTTAATGGCGCGGCAGGCGTCCATCAGGCGGCGAACGGGCTGGTAAACCCACCACGGCACCAGGTTTTCGGCGCTCATCAGGCCAAAGGGGATgg                                              >  1:147124/1‑248 (MQ=255)                                                                                                                                                                                                 gggccgggcTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCC‑GATGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGGGCCGAGGCCGACGGCGACCACGAACAGCATGGCGAAGACCATTTCGTTAATGGCGCGGCAGGCGTCCATCAGGCGGCGAACGGGCTGGTAAACCCACCACGGCACCAGGTTTTCGGCGCTCATCAGGCCAAAGGGGATGGAGAGAaccacc                                   <  1:231467/251‑1 (MQ=255)                                                                                                                                                                                                     cgggcTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCC‑GATGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGGGCCGAGGCCGACGGCGACCACGAACAGCATGGCGAAGACCATTTCGTTAATGGCGCGGCAGGCGTCCATCAGGCGGCGAACGGGCTGGTAAACCCACCACGGCACCAGGTTTTCGGCGCTCATCAGGCCAAAGGGGATGGAGAGAACCACCGCCa                               <  1:209639/251‑1 (MQ=255)                                                                                                                                                                                                           cactCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCCCGCC‑GTTGGTGTGGATAAACAGCGCCAGGACGCCGGCACGCCGGCGAGCGGGCCGAGGCCGACGGCGACCACGAACAGCATGGCGAAGCCCATTTCGTTAATGGCGCGGCAGGCGTCCGTCAGGCGGGGAACGGGTGGGTAAACCCACCACGGCACCAGGTTTCCGGCGCTCATCAGGCCAAAGGGGATGGAGAGACCCACCGCCAGCGCgg                         <  1:134256/248‑1 (MQ=255)                                                                                                                                                                                                            aaTCGCTTCCGCCGCTTCGGAAAGCAGCTGGGAGAGC‑CGCCTGAGGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGACCGGGCCGAGGCCGACGGCGACCACGAACAGCATGGCGAAGACCATTTCGTGAATGGCGCGGCAGGCGTCCATCAGGCGGCGAACGGGCTGGTAAACCCACCACGGCACCAGGTTTTCGGCGCTCATCAGGCCAAAGGGGATGGAGAGAACCACCGCAAGCGCGGt                        <  2:187370/251‑1 (MQ=255)                                                                                                                                                                                                                   cccACCGCTTGGGAAAGCAGCTTGGAGAGACCGCC‑GATGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGGGCCGAGGCCGACGGCGACCACGAACAGCATGGCGAAGACCATTTCGTTAAGGGCGCGGCATGCGTCCATCAGTCGGCTAACGGGCTGATAAACCCTCCACGGCACCAGGTTTGCGGCGCTCATCAGGCCAAAGGGGATGGAAAGAACCACCGCCAGCGCGGTGCCCCAg                 <  2:159705/250‑1 (MQ=255)                                                                                                                                                                                                                        cGCTTCGGAAAGCAGCTTGGAGAGCACGCC‑GATGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGGGCCGAGGCCGACGGCGACCACGAACAGCATGGCGAAGACCATTTCGTTAATGGCGCGGCAGGCGTCCATCAGGCGGCGAACGGGCTGGTAAACCCACCACGGCACCAGGTTTTCGGCGCTCATCAGGCCAAAGGGGATGGAGAGAACCACCGCCAGCGCGGTGCCCCAGACGGc            >  1:60172/1‑251 (MQ=255)                                                                                                                                                                                                                             cgaaaaGCAGCTGGGAAAGCGCGCC‑GATGGTTGGGATAAACACCGCCAGCACGCCAGCACGCCAGCGAACGGGCCGAGGCCGACGCCGACCACGAACAGCATGGCGAAGACCATTTCGTTAATGGCGCGGCAGGCGGCCAGCAGGCGGGGAAGGGGCTGGGAAACCCACCACGGCACCAGGTTTGCGGCGCTCATCAGGCCAAAGGGGATGGAGAGAACCACCGCCAGCGCGGTGCCCCAGACGGCGAttt       <  1:252258/248‑1 (MQ=255)                                                                                                                                                                                                                              ggAAAGCAGCTTGGAGACCCTGCA‑GATGGTGTGGATAAATACCGCCAGCCCGCCATCACTCCAGCGAGCGGTCCGAGGCCGACGGCGCCCACGAACAGCATGGCGTAGACCATTTCGTTATTGTCGCGGCAGGCGTCCATCAGGCGGCGAACGGGCTGGTAAACCCACCACGGCCCCAGGTTTGCGGCGCTCATCAGGCCTATGGGGATGGAGAGAACCACCGCCAGCGCGGTGCCCCAGGCGGCGATTTg      <  1:181377/251‑1 (MQ=255)                                                                                                                                                                                                                                 aaGCAGCTTGGAGAGCACGCC‑GATGGTGTGGATAAACAGCGCAAGCACGCCAGCACGCCAGCGAACGGGCCGAGGCCGACGGCGACCACGAACAGCATGGCGAAGACCATTTCGTTAATGGCGCGGCAGGCGTCCATCAGGCGGCGAACGGGCTGGTAAACCCACCACGGCACCAGGTTTTCGGCGCTCATCAGGCCAAAGGGGATGGAGAGAACCACCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAg   <  1:217715/251‑1 (MQ=255)                                                                                                                                                                                                                                  agcagcTTGGAGAGCACGCC‑GATGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGGGCCGAGGCCGACGGCGACCACGAACAGCATGGCGAAGACCATTTCGTTAATGGCGCGGCAGGCGTCCATCAGGCGGCGAACGGGCTGGTAAACCCACCACGGCACCAGGTTTTCGGCGCTCATCAGGCCAAAGGGGATGGAGAGAACCACCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGc  >  1:11596/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                      AAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTCGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCC‑GGTGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGGGCCGAGGCCGACGGCGACCACGAACAGCATGGCGAAGACCATTTCGTTAATGGCGCGGCAGGCGTCCATCAGGCGGCGAACGGGCTGGTAAACCCACCACGGCACCAGGTTTTCGGCGCTCATCAGGCCAAAGGGGATGGAGAGAACCACCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGC  >  NC_000913/4322540‑4323015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑