Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,321,603	T→C	I32V (ATC→GTC)	phnG ←	carbon‑phosphorus lyase core complex subunit PhnG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,321,603	0	T	C	100.0%	205.4 / NA	68	I32V (ATC→GTC)	phnG	carbon‑phosphorus lyase core complex subunit PhnG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/31);  total (37/31)

TCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGATGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTC‑AGTGCTCCATAGTGAATTCAATCATGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCT  >  NC_000913/4321359‑4321848                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       tCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTa                                                                                                                                                                                                                                                   <  1:209395/250‑1 (MQ=255)     aTAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCCGTCAGGCGCACGGCGCCGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGCATCTGTACCAGGCCAGGTTCAGCGGCGCGGCTCACCTCATCGACGGCGGTGGCGTTTAGcgcg                                                                                                                                                                                                                                              >  2:61721/1‑251 (MQ=255)         gTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCTGTGACGTTTAGCGCGTTCa                                                                                                                                                                                                                                          <  2:262246/251‑1 (MQ=255)         gTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCa                                                                                                                                                                                                                                          <  1:55414/251‑1 (MQ=255)                    ggCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGTTCGGCGAGCTGTTTATCACGCCCCTGCCCCCCGCTGTAGCAGAGCGTGCCGTCAGCCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGCAGCCGCCCATCCGCGCCTGCATCTGTACCAGGCCAGATTACGCGGCGCGGCCCACCTCATAGTCGGCGGTGACGTTTAGCGAGTTCAGGCGTGCTGcc                                                                                                                                                                                                                               >  1:124538/1‑251 (MQ=255)                      cGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGcc                                                                                                                                                                                                                               >  2:208241/1‑249 (MQ=255)                       gagaTTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGGTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGTTGTAGTCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCGGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCGGTACCAGGCCAGTTTCAGCGGCGCGGATCACCGCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGt                                                                                                                                                                                                                            <  1:266361/251‑1 (MQ=255)                        agaTTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCCGGCTTGCTGCCCGtt                                                                                                                                                                                                                           >  2:38912/1‑251 (MQ=255)                           ttGCTGCATAAGCGCGTCAATCAGCGCGCAGCGTTCAGCGTGCTGTTTATCACGCCACTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATACTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGACAGTTc                                                                                                                                                                                                                          >  2:188838/1‑249 (MQ=255)                            tgctgcATCAGCGAGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGGGACGTTTAGCGCGTTCAGGCGTGCTGCCCGTTCAGc                                                                                                                                                                                                                       >  2:267196/1‑251 (MQ=255)                                   tCAGCGCGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTgg                                                                                                                                                                                                                >  1:151797/1‑251 (MQ=255)                                         cgTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTCTCACGCCCCTGCACCCAGATGTAGCCGCGCGTGCCGTCCGTCCGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCCTCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGGTCACCTCCTAGTCGGCGGCGACGTTTAGGGCATTCCGGCGGGCTGCCAGCTAAGCCGGTTGGCTGTgc                                                                                                                                                                                                          >  2:123528/1‑251 (MQ=255)                                          gTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTgcg                                                                                                                                                                                                         >  1:133951/1‑251 (MQ=255)                                                       cAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCCCGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAACAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGCATCTGTACCAGGCCAGTTTCAGCGGCGCGGCTCACCTCATAGTCGGCGGTGACGTTTCGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTGTGCTGTGCGCCAGCACGGCCAt                                                                                                                                                                                            >  2:133354/1‑251 (MQ=255)                                                           gTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATcc                                                                                                                                                                                          >  1:40972/1‑249 (MQ=255)                                                              cGGCGTGCTGGTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGc                                                                                                                                                                                     <  2:213290/251‑1 (MQ=255)                                                                gCGTGCTGTTTATCACGCCCCAGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGGGCGCCAGCACGGACAGCCAgctctg                                                                                                                                                                                   >  2:53217/1‑246 (MQ=255)                                                                 cGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACCTCCAGTGCTgg                                                                                                                                                                                  >  1:170651/1‑251 (MQ=255)                                                                 cGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTgg                                                                                                                                                                                  >  1:102293/1‑251 (MQ=255)                                                                         gtATCACGCCCCGGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTcg                                                                                                                                                                           <  2:264435/249‑1 (MQ=255)                                                                           aTCACGCCCCTGCACCCAGCTGTCGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGGGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGGCGCgg                                                                                                                                                                        >  2:21521/1‑251 (MQ=255)                                                                            ccACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGt                                                                                                                                                                       <  1:162182/250‑1 (MQ=255)                                                                               cGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATc                                                                                                                                                                    <  2:31731/251‑1 (MQ=255)                                                                                gCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGCCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTGCCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTTGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCt                                                                                                                                                                   <  1:175826/251‑1 (MQ=255)                                                                                             gCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTC‑AGt                                                                                                                                                     <  1:90837/251‑1 (MQ=255)                                                                                                gTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTC‑AGTg                                                                                                                                                    <  1:62603/249‑1 (MQ=255)                                                                                                gTAGCCGAGCGGGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTC‑AGTGCt                                                                                                                                                  <  2:59432/251‑1 (MQ=255)                                                                                                 gagcggtgCGTGCCGTCAGGCAGGCGCACGGCGACGCGGGTCAGCGGGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTTCCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTGGCGGTATCTGCGTGCAGTC‑AGTGCTc                                                                                                                                                 <  2:42072/244‑1 (MQ=255)                                                                                                     cGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTGTCTGCGTGCATTC‑AGTGATCCAt                                                                                                                                              >  1:132416/1‑250 (MQ=255)                                                                                                          gTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGGGCTGGCGGGGCCCGGTATCTGGGTGGATTC‑CGGGCTcccttgtga                                                                                                                                         >  2:8513/1‑243 (MQ=255)                                                                                                               gtcggtccggCGCACGGCGCCGGGGGCCACCGTGGCATCGCCTGCTAAAAACCGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGTCCATTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGTTATCTGCGTGCATTC‑AGTGCTCCATAGTGAATTCaa                                                                                                                                   <  2:221193/243‑1 (MQ=255)                                                                                                               gtcagtcaGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCCTTC‑AGTGCCCCATAGTGAATTCa                                                                                                                                    >  1:139255/1‑250 (MQ=255)                                                                                                               ggcaggcagGCGCACGGCGACGCGGGTCAGCGTGGCGGCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTC‑AGTGCTCCATAGTGAATTCaa                                                                                                                                   <  2:198222/245‑1 (MQ=255)                                                                                                                 cagtcagGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGGCGCGGGATCTGCGTGCGTTCTAG‑GCCCCATAGTGGATTCAAt                                                                                                                                  >  2:29207/1‑250 (MQ=255)                                                                                                                     cagGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTGTCTGCGGGCATTC‑CGTGCTCCCTAGAGAATTTAAACATGt                                                                                                                             >  1:50434/1‑251 (MQ=255)                                                                                                                        gcgcACGGCGCCGCTGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGGTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTC‑AGTGCTCCATAGTGAATTCAATCATGTCgg                                                                                                                          <  2:1383/251‑1 (MQ=255)                                                                                                                         cgcACGGCGACGCGGGTCAGCGGGGCGGCGCCGGAAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGGACCAGGCCAGTTTCAGCGGCGCGGATCACCTCAGAGTCGGCGGTGACGTTTAGCGCGGTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTC‑AGTGCTCCATAGTGAATTCAATCATGTCGgc                                                                                                                         <  1:106677/251‑1 (MQ=255)                                                                                                                                cgacgcgGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGGCGCGGTATCTGCGTGCATTC‑AGTGCTCCATAGTGAATTCAATCATGTCGGCGCGCGTc                                                                                                                  >  1:189146/4‑251 (MQ=255)                                                                                                                                 gacgcgGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGGATCTGCGTGCCTTC‑AGTGCTCCCTAGTGAATTCCATCATGTCGGCGCGCGTcc                                                                                                                 >  1:74031/3‑250 (MQ=255)                                                                                                                                  acgcgGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGGCGCGGAATCTGCGTGCCTTC‑AGGGCTCCATAGGGAATTCAAACATGTCggcgggcgcccg                                                                                                                >  1:234050/2‑247 (MQ=255)                                                                                                                                          caccGTGGCGTCGCCGGCAAAAAAACGTTCGCCGTTGCCGACCACCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCAGAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGCCATCCAGTGCTGGCGGGTCGTGGTATCTGCGTGCATTC‑AGTGCTCCATAGGGACTTCAATCATGTCGGCGCGCGTCAGGCTGACg                                                                                                         <  2:208759/247‑1 (MQ=255)                                                                                                                                          cAGCGTGGCGGCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTC‑AGTGCTCCATAGTGAATTCAATCATGTCGGCGCGCGTCAGGCTGACgg                                                                                                        <  2:66304/251‑1 (MQ=255)                                                                                                                                                       ccGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGGCGCGGGATCTGCGTGCATTC‑AGTGCTCCATAGTGAATTCAATCATGTCGGCGCGCGTCAGGGCGCAGGGGTACTCCGCCgg                                                                                           >  2:203027/1‑251 (MQ=255)                                                                                                                                                       ccGGCAAAAAAACGTGCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTC‑AGTGCTCCATAGTGAATTCAATCATGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCgg                                                                                           <  2:205190/251‑1 (MQ=255)                                                                                                                                                        cgacAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTTCATGC‑AGTGCTCCATAGTGAATTCAATCATGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCggg                                                                                          <  2:234050/248‑1 (MQ=255)                                                                                                                                                                 aacgtgagCCGGTGCCGCCCAGCCGCGCCTGAATCTGGACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGGCGGCGGTGACGTTTAGCGCGGTCAGGCGTGCTGCCAGTGCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGGGCTGGCGGGTCGCGGTATCTGCGTGCATTC‑AGGTCACCATAGTGAATTCAATCATGTCGGCGCGCGTCAGGCTGACGTAGTACTCCGCCGGTCTGCTTTCa                                                                                 <  2:114105/244‑1 (MQ=255)                                                                                                                                                                  caccgccgactatgaggtgaTCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGAATCTGCGTGCATTC‑AGTGCTCCATAGCGAATTCAATCATGTCGGCGCGCGTCAGGCTGACGGAGTACTCCCCCGGGCTGCTTTCAc                                                                                >  1:133968/20‑251 (MQ=255)                                                                                                                                                                        gCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATCGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGGATCTGCGTGCATTC‑CGTGCTCCATAGTGAATTCAATCATGTCGGCGCGCGTCCGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCAc                                                                          >  1:38752/1‑251 (MQ=255)                                                                                                                                                                           ggTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGGGGCTTC‑AGTGCTCCATAGTGAATTCAATCATGTCGGCGCGCGTCAGGCTGACGGAGTACTCCTCCGGGCTGCTTTCACCGTCACggg                                                                       >  1:151162/1‑250 (MQ=255)                                                                                                                                                                           ggTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGGCGCGGGATCTTCGGGCCTTC‑AGTGCTCCATAGTGAATTCCATCATGGCGGCGCGGGGCAGGATGACGGAGGAACCCGCCGGGCTGCTTTCTCCCTCACggg                                                                       >  2:212110/1‑250 (MQ=255)                                                                                                                                                                              gccgccCACCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGGCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTC‑AGTGCTCCATAGTGAATTCAATCATGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACggtgg                                                                     <  2:43622/250‑1 (MQ=255)                                                                                                                                                                               ccgccCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTC‑AGTGCTCCATAGTGAATTCAATCATGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGtt                                                                   <  1:264913/251‑1 (MQ=255)                                                                                                                                                                                     aTCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTC‑AGTGCTCCATAGTGAATTCAATCATGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGt                                                             <  2:74031/251‑1 (MQ=255)                                                                                                                                                                                       ccGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGGTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTC‑AGTGCTCCATAGTGAATTCAATCATGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTgc                                                           <  2:237422/251‑1 (MQ=255)                                                                                                                                                                                           gcCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTC‑AGTGCTCCATAGTGAATTCAATCATGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGcgcac                                                       <  1:218371/251‑1 (MQ=255)                                                                                                                                                                                           gcCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGGCGCGGGATCTGCGTGCCTTC‑CGTGCTCCATAGGGGATTCAATCATGTCGGCGCGCGTCAGGCTGACGGGGTACTCCGCCGGGCTGCTTTCTCCCTCACGGGGGGTAAGGGGGcgcac                                                       >  2:133163/1‑251 (MQ=255)                                                                                                                                                                                             cTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTC‑AGTGCTCCCTAGGGAATTCAATCATGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGGCACGGTGGGTAAGGGTGcgcacgc                                                     >  1:173695/1‑251 (MQ=255)                                                                                                                                                                                                  tCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTC‑AGTGCTCCATAGTGAATTCAATCATGTCGGCGCGCGTCAGGCTGACGGAGTAATCCGCCGGGCTGCTTTCACCGTCCCGGGGGGTAAGGGTGCGCACGCAcagc                                                >  1:242760/1‑251 (MQ=255)                                                                                                                                                                                                     gTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTC‑AGTGCTCCATAGTGAATTCAATCATGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCAcag                                                 <  1:43408/247‑1 (MQ=255)                                                                                                                                                                                                         cAGGCCAGTTTCAGCGGCGCGTATCACCTCATAGTCGGCGGTGACGGTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTC‑AGTGCTCCATAGTGAATTCAATCATGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAgcggcg                                         <  1:11896/251‑1 (MQ=255)                                                                                                                                                                                                             ccAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTAACTGCGGGCATTC‑CGTGCTCCATAGTGAATTCAATCCTGTCGGCGCGCGTCAGGCTGCAGGGGTACTCCGCCGGGCTGCTTTCACCGTCCCGGGGGGTAAGGGTGCGCAAGCACAGCAgcggggcga                                      >  1:138385/1‑245 (MQ=255)                                                                                                                                                                                                                gTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTC‑AGTGCTCCATAGTGAATTCAATCATGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATAtt                                  <  1:133354/251‑1 (MQ=255)                                                                                                                                                                                                                        ggcgCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTC‑AGTGCTCCATAGTGAATTCAATCATGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGGGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGGttt                          >  1:101408/1‑251 (MQ=255)                                                                                                                                                                                                                                tCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGATGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGGCGAGGTCTCTGCGTGCATTC‑AGTGCTCCATAGTGAATTTAATAATGTCGGGGGGCGGCAGGCTGACGGCGTAATCCCCCCGGCCGCTTTCCCCGTCACGGTGGTTTAGGGGGCGCCCGCACCGCCGCGGCGGCATATTctggggttgccgacgg                  >  2:178251/1‑236 (MQ=255)                                                                                                                                                                                                                                      cATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTC‑AGTGCTCCATAGTGAATTCAATCATGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCa             <  1:61721/250‑1 (MQ=255)                                                                                                                                                                                                                                      cATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGGATCTGCGGGCATTC‑AGTGCTCCATAGTGAATTTAATCATTTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCCCCGCCACGGGGGTTTAGGGGGCGCACGCACCGCAGCGGCGACCTATTCGGGGTTTTCAGACGCTGGccc            >  1:177794/1‑249 (MQ=255)                                                                                                                                                                                                                                         aGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGGATCTGCGTGCATTC‑AGTGCTCCATAGTGAATTCAATCATGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCCGCGGCGGCATATTCGGGGTTTCAAAACGCTGGCActct         >  1:101422/1‑251 (MQ=255)                                                                                                                                                                                                                                                ggTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGGATCTGCGTGCCTTC‑CGTGCTCCATAGTGCATTCAATCATGTCGGCGCGCGTCAGGCTGCCGGAGTACTCCGCCGGGCTGCTTTCACCATCACGGGGGTTAAGGGTGCGCACGCACAGCAGCGGCGCCATATTCGGGATTTCCCAACGCTGGCACTCTTTTGccc  >  2:66184/1‑250 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       TCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGATGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTC‑AGTGCTCCATAGTGAATTCAATCATGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCT  >  NC_000913/4321359‑4321848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑