Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,298,401	G→A	L322L (CTC→CTT)	fdhF ←	formate dehydrogenase H

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,298,401	0	G	A	100.0%	138.2 / NA	44	L322L (CTC→CTT)	fdhF	formate dehydrogenase H
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/21);  total (23/21)

TCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACGCCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTGCCGCCTGACGAATCTCACTGGCGCTGACGCCGGTGATATCTTCAACCGACTCCGGCGTGTAGCCTTCAACGATTTTACGATACTCTTCAAAGCCTTCTGTACGTGAAGCGACGAACGCTTTGTC  >  NC_000913/4298156‑4298646                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      tCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCCCGAACCGGGTTAACACCCGCATGCGGCTTACCAAGGtt                                                                                                                                                                                                                                                  >  2:5459/1‑251 (MQ=255)   ttCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCAAGGTTAc                                                                                                                                                                                                                                                >  1:43002/1‑251 (MQ=255)        ccATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCAAGGTTACCGGTc                                                                                                                                                                                                                                           <  1:179696/251‑1 (MQ=255)         cATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGACCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTACACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTCCCAAGGTTACCGGTcc                                                                                                                                                                                                                                          >  2:163654/1‑250 (MQ=255)                      gcacgcacTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCATGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCAAGGTTACCGGTCAGCATCGCGAGGCt                                                                                                                                                                                                                             <  2:203379/251‑1 (MQ=255)                       cacgcacTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGCTCGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTCTCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCAAGGTTACCGGTCAGCATCGCGAGGCTg                                                                                                                                                                                                                            >  1:127177/1‑251 (MQ=255)                       cacgcacTTCGCCAGGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGGTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCAAGGTTACCGGTCAGCATCGCGAGGCTg                                                                                                                                                                                                                            <  1:164433/251‑1 (MQ=255)                           cacTCCGCCATGCGCTGCCCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGTCTTTCCCCGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCAAGGTTACCGGGCAGCATCGCGAGGCTGGt                                                                                                                                                                                                                          <  2:64571/249‑1 (MQ=255)                                  ccATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCAAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACg                                                                                                                                                                                                                 >  1:165527/1‑251 (MQ=255)                                      gcgctgcgcGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCAAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCAc                                                                                                                                                                                                              <  1:218576/250‑1 (MQ=255)                                                      cAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCAAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACGCCCTGGt                                                                                                                                                                                             >  2:235336/1‑251 (MQ=255)                                                                       aGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCAAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACGCCCTGGTAGAACTGGGTTACAccc                                                                                                                                                                            >  1:221015/1‑251 (MQ=255)                                                                              aTGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCCCGGTTAGCCGGATCTTTCCCGGACTGGTATCCCGGATACGTCTCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTCGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCAAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCCCGGTTTCCACGCCACGGAAGAACTGGGTTAAACCCATGcccc                                                                                                                                                                     >  2:98866/1‑251 (MQ=255)                                                                                                cACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCAAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACGCCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCttt                                                                                                                                                   <  2:147715/251‑1 (MQ=255)                                                                                                                 cGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCAAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACGCCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATc                                                                                                                                  >  2:122807/1‑251 (MQ=255)                                                                                                                    aTTTCTCGCGGGTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGCACGTTGTTCTGACCACGACCCGGGTTAACACCCGCATGCGGCTTACCAAGGTTACCGGTAAGCCTCGCGAGGCTGGTCAGCGAACGCACGGTTTCCACGCCCTGGCAGAACTGGGTTACACCCATGCCCAACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGt                                                                                                                               >  2:77721/1‑251 (MQ=255)                                                                                                                     tttCTCGCGGTTAGCCGGATCTTTCACGTACTACTACCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGACCCAGGTTAACACCCGCATGCGGCTTACCAAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGACCGCCCGGTTTCCAAGCCCTGGGAGAACTGGGTTCAACCCATGACCCACAGTATGGCGGCGCTTTTCGCATGGGCATAcctcggtg                                                                                                                              >  2:94897/1‑244 (MQ=255)                                                                                                                      ttCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTGCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCAAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACGCCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTg                                                                                                                              <  2:43002/250‑1 (MQ=255)                                                                                                                            cgGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCAAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACGCCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGCTGGCGGCGCTTTTCGCCTGGGCATACATCCGTGCCGCCTg                                                                                                                       >  2:77040/1‑251 (MQ=255)                                                                                                                                 aGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCAAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACGCCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTGCCGCCTGACGa                                                                                                                   >  2:125587/1‑250 (MQ=255)                                                                                                                                     ggATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCAAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACGCCCTGGTAGCACTGGGTTACACCCATGCCCCACAGGCTGGCGGCGCTTTTCGCCTGGGCATACATCCGTGCCGCCTGACGAAtctc                                                                                                              >  1:55912/1‑251 (MQ=255)                                                                                                                                           ttCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCAAGGTTACCGGTCAACATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACGCCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTGCCGCCTGACGAATCTCACTgg                                                                                                         <  1:189373/250‑1 (MQ=255)                                                                                                                                                     ggTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCAAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACGCCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTGCCGCCTGACGAATCTCACTGGCGCTGACGcc                                                                                               <  1:125587/250‑1 (MQ=255)                                                                                                                                                                 aCGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCAAGGTTACCGGTCAGCATCGCGATGCTGGTCAGAGAACGCACGGTTTCCACGCCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTCTTCGCCTGGGCATACATCCGTGCCGCCTGACGAATCTCACTGGCGCTGACGCCGGTGATATCTTCa                                                                                  <  1:150949/251‑1 (MQ=255)                                                                                                                                                                    tATCCGGGAGCGCGAGCATATCGCAGGCACCCTGAACGTTGTTCTGACCCCGAACCGGGTTAACACCCGCATGCGGCTTACAAAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACGCCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGCTGGCGGCGCTTTTCGCCTGGGCATACATCCGTGCCGCCTGACGAATCTCACTGGCGCTGACGCCGGTGATATCTTCAAcc                                                                               >  2:115970/1‑251 (MQ=255)                                                                                                                                                                       ccGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCAAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACGCCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTGCCGCCTGACGAATCTCACTGGCGCTGACGCCGGTGATATCTTCAACCGAc                                                                            <  1:135138/251‑1 (MQ=255)                                                                                                                                                                           cAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCAAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACGCCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTGCCGCCTGACGAATCTCACTGGCGCTGACGCCGGTGATATCTTCAACCGACTCCg                                                                        <  2:54854/251‑1 (MQ=255)                                                                                                                                                                                   ccATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCAAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACGCCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTGCCGCCTGACGAATCTCACTGGCGCTGACGCCGGTGATATCTTCAACCGACTCCGGCgtgt                                                                  >  2:185298/1‑249 (MQ=255)                                                                                                                                                                                    cATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCAAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACGCCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTGCCGCCTGACGAATCTCACTGGCGCTGACGCCGGTGATATCTTCAACCGACTCCGGCGTGTAGc                                                               >  2:80356/1‑251 (MQ=255)                                                                                                                                                                                         cGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCAAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACGCCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTGCCGCCTGACGAATCTCACTGGCGCTGACGCCGGTGATATCTTCAACCGACTCCTGCGTGTAGCCTTCa                                                          <  2:165527/251‑1 (MQ=255)                                                                                                                                                                                                 cccTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCAAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACGCCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTGCCGCCTGACGAATCTCACTGGCGCTGACGCCTGTGATATCTTCAACCGACTCCGGCGTGTAGCCTTCAACGAtttt                                                  <  1:163654/251‑1 (MQ=255)                                                                                                                                                                                                     gAACGTTGTTCGGAACACGAACCGGGTTAACTCCCGCATGCGGCTTACCAAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACGCCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTGCCGCCTGACGAATCTCACTGGCGCTGACGCCGGTGATATCTTCAACCGACGCCGGCGTGTAGCCTTCAACGATTTTACg                                               <  2:174090/250‑1 (MQ=255)                                                                                                                                                                                                      aaCGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCAAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACGCCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTGCCGCCTGACGAATCTCACTGGCGCTGACGCCGGTGATATCTTCAACCGACTCCGGCGTGTAGCCTTCAACGATTTTACGAt                                             <  1:235336/251‑1 (MQ=255)                                                                                                                                                                                                                  aCCACGAACCGGGTTAACACCCGCATGCGGCTTCACAAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACGCCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTGCCGCCTGACGAATCTCACTGGCGCTGACGCCGGTGATATCTTCAACCGACTCCGGCGTGTAGCCTTCAACGATTTTACGATACTCTTCaagcc                                 <  1:142635/251‑4 (MQ=255)                                                                                                                                                                                                                       gaacccgggtcgcacacGCATGCGGCTGACCAAGGTTACCGGTCAGCATAGCGAGGCTGGTAAGAGAACGCACGGGTTCCACGACCTGGAAGAACTGGGTCACACCCATGCCCCACAGGATGGCGGCGCTTTTAGCCTGGGCATACATCCGAGCCGCCTGACGAATCTCACTGGCGCTGACACCGGTGATATCTTCAACCGACTCCGGCGTGTAGCCTTCAACGGTTTTACGATAATATTCAAAGCCttat                            >  2:158669/17‑249 (MQ=255)                                                                                                                                                                                                                         aCCGGGTTAACACCAGCATGCGGCTTACCAAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACGCCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTGCCGCCTGACGAATCTCACTGGCGCTGACGCCGGTGATATCTTCAACCGACTCCGGCGTGTAGCCTTCAACGATTTTACGATACTCTTCAAAGCCTTCTGt                          >  1:168906/1‑251 (MQ=255)                                                                                                                                                                                                                          ccGGGTTAACACCCGCATGCGGCTTACCAAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTGCCACGCCCTGGTAGAACTGGGTTACTCCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTGCCGCCTGACGAATCTCACTGGCGCTGACGCCGGTGATAGCTTCAACCGACTCCGGCGTGTAGCCGTCAACGATTGTACGATTCTCTTCAAAGCCTTCTGTa                         <  2:127177/251‑1 (MQ=255)                                                                                                                                                                                                                          ccGGGTTAACACCCGCATGCGGCTTACCAAGGTTACCGGTAAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACGCCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTGCCGCCTGACGAATCTCACTGGCGCTGACGCCGGTGATATCTTCAACCGACTCCGGCGTGTAGCCTTCAACGAGTTTACGATACTCTGCAAAGCCTTCTGTa                         <  2:256118/251‑1 (MQ=255)                                                                                                                                                                                                                            gggTTAACACCCGCATGCGGCTTACCAAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACGCCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTGCCGCCTGACGAATCTCACTGGCGCTGACGCCGGTGATATCTTCAACCGACTCCGGCGTGTAGCCTTCAACGATTTTACGATACTCTTCAAAGCCTTCTGTACg                       >  2:101394/1‑251 (MQ=255)                                                                                                                                                                                                                                 aaCACCCGCATGCGGCTTACCAAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACGCCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTGCCGCCTGACGAATCTCACTGGCGCTGACGCCGGTGATATCTTCAACCGACTCCGGCGTGTAGCCTTCAACGATTTTACGATACTCTTCAAAGCCTTCTGTACGTGAAg                  <  1:101394/251‑1 (MQ=255)                                                                                                                                                                                                                                      ccGCATGCGGCTTACCAAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACGCCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTGCCGCCTGACGACTCTCACTGGCGCTGACGCCGGTGATATCTTCAACCGACTCCGGCGTGTAGCCTTCAACGCTTTTACGCTACTCTTCAAAGCCTTCTGTACGTGAAGcgacg             >  2:218500/1‑251 (MQ=255)                                                                                                                                                                                                                                       cGCATGCGGCTTACCAAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACGCCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTGCCGCCTGACGAATCTCACTGGCGCTGACGCCGGTGATATCTTCAACCGACTCCGGCGTGTAGCCTTCAACGATTTTACGATACTCTTCAAAGCCTTCTGTACGTGAAGcgacg             >  1:166190/1‑250 (MQ=255)                                                                                                                                                                                                                                          aTGCGGCTTACCAAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACGCCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTGCCGCCTGACGAATCTCACTGGCGCTGACGCCGGTGATATCTTCAACCGACTCCGGCGTGTAGCCTTCAACGATTTTACGATACTCTTCAAAGCCTTCTGTACGTGAAGCGACGAACg         <  1:60491/251‑1 (MQ=255)                                                                                                                                                                                                                                                 ttACCAAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACGCCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTGCCGCCTGACGAATCTCACTGGCGCTGACGCCGGTGATATCTTCAACCGACTCCGGCGTGTAGCCTTCAACGATTTTACGATACTCTTCAAAGCCTTCTGTACGTGAAGCGACGAACGCTTTGTc  >  1:34774/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      TCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACGCCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTGCCGCCTGACGAATCTCACTGGCGCTGACGCCGGTGATATCTTCAACCGACTCCGGCGTGTAGCCTTCAACGATTTTACGATACTCTTCAAAGCCTTCTGTACGTGAAGCGACGAACGCTTTGTC  >  NC_000913/4298156‑4298646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑