Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,285,238	G→A	intergenic (‑25/+175)	yjcH ← / ← acs	DUF485 domain‑containing inner membrane protein YjcH/acetyl‑CoA synthetase (AMP‑forming)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,285,238	0	G	A	100.0%	96.2 / NA	31	intergenic (‑25/+175)	yjcH/acs	DUF485 domain‑containing inner membrane protein YjcH/acetyl‑CoA synthetase (AMP‑forming)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/14);  total (17/14)

ACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCC  >  NC_000913/4285002‑4285466                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                     aCTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAAGGATTGTTTTAAtt                                                                                                                                                                                                                        >  1:151372/1‑251 (MQ=255)                ggAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAAGGATTGTTTTAATTCCCGCTCCCTTATgg                                                                                                                                                                                                         >  1:38660/1‑251 (MQ=255)                     aCCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAAGGATTGTTTTAATTCCCGCACCCTTATGGGAGaa                                                                                                                                                                                                    <  2:38660/251‑1 (MQ=255)                                ggcgggtggtCGGATGCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGGAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAAGGATTGTTTTAATTCCCGCTCCCTTAGGGGAGAAGGTTAACGCTc                                                                                                                                                                                         <  2:72523/247‑1 (MQ=255)                                         tCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATAGAAACTGCCATCATAATAATCGTCAGGATGGTGGCAAACCTTTGCCGTTTTTCTACTAACGCCCTGAAATGCTCATTGTCTTCTATCCTCTGATAAATAGTTCCATGCATCACAGATTCTCCAGATGTAATGTAAGGATTGGTTTAGGTCGCGCTTCCTTATGGGTGAAgctttgcggtctgttaacc                                                                                                                                                                                 <  2:175776/250‑19 (MQ=255)                                            gATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAAGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTg                                                                                                                                                                             <  2:75926/251‑1 (MQ=255)                                                    ggcgTACCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGACATAAACTGCCAGCCTAATCCTCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCCTTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAAGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGt                                                                                                                                                                     >  2:136831/1‑251 (MQ=255)                                                                    cGGGCGCGACGGCGATCAGTAAAATACAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAACTGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAAGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGt                                                                                                                                                     >  2:111006/1‑251 (MQ=255)                                                                         gcgAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAAGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTAcg                                                                                                                                                >  1:1543/1‑251 (MQ=255)                                                                         gcgAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATACGCTGCTAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAAGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGtttccg                                                                                                                                                >  1:249209/1‑248 (MQ=255)                                                                              ggCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAAGGATTGTTTTACTTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTAcgccgc                                                                                                                                            >  1:20919/1‑250 (MQ=255)                                                                                    cAGTAAACTAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCAGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGGAATGTAAGGAATGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGACGCATCCGGc                                                                                                                                     >  1:199416/1‑251 (MQ=255)                                                                                    cAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAAGAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAAGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCGCGGGTAACCCTTGCCGAATTTAGGCCGGATAAGGCGTTTACGCCGCATCCgg                                                                                                                                      <  2:46592/250‑1 (MQ=255)                                                                                     aGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAAGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGc                                                                                                                                     >  1:209692/1‑250 (MQ=255)                                                                                           aTAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAAGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGcaatcaat                                                                                                                              >  2:89875/1‑251 (MQ=255)                                                                                             aaaGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAAGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGc                                                                                                                            >  1:176118/1‑251 (MQ=255)                                                                                                              ccAGCATAATAAGCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCAGTGTCTTCTAGCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAAGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACTCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTcg                                                                                                           <  2:251276/251‑1 (MQ=255)                                                                                                               cAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAAGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTcgc                                                                                                          <  2:20919/251‑1 (MQ=255)                                                                                                                aGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGAAAGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGTTGCGACGCTGTcgcg                                                                                                         >  1:143218/1‑251 (MQ=255)                                                                                                                              cAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAAGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCt                                                                                            >  2:70248/1‑250 (MQ=255)                                                                                                                                            aCCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGGGCCATTCATCACAGATTCTCCAGAGGTAATGTAAGGATTGTTTTAGTTCCCGCTCCCTTATGGAAGAAAGTTAACGCTCGGGTAACCCTTGACGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCCATGCCTGATGCGACGCTGTAGCGTCTTATCAGGCCTACAAACCGTTACCg                                                                              >  1:117571/1‑250 (MQ=255)                                                                                                                                               gTTGCCGTTTTTCGACTAACTGCCTGAAATGCGCATTGTTTTCTATCCGCTGATAAATAGTGCCATTCACCACAGATGCTCCAGAGGGAATGTGAGGATTTTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGTTAACCCGTGCCGAATTTATGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATTCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAAACCGTTACCGACTc                                                                          <  1:140287/251‑1 (MQ=38)                                                                                                                                                 tGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAAGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAAACCGTTACCGACTCg                                                                         <  1:70193/250‑1 (MQ=255)                                                                                                                                                                     ccTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAAGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTATCCCTTGCCGAATGTAGGCCGGATAAGGCGTTTATGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCTTACAAACCGTTTCCGACTCGCATCGGGCAATTGTGGGTTAc                                                    <  1:194979/251‑1 (MQ=255)                                                                                                                                                                        gAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAAGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACg                                                   <  2:1543/249‑1 (MQ=255)                                                                                                                                                                                cATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAAGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATcgc                                         <  2:664/251‑1 (MQ=255)                                                                                                                                                                                       ttctATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAAGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGcc                                  >  1:65662/1‑251 (MQ=255)                                                                                                                                                                                           aTCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAAGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCt                                 <  2:65662/248‑1 (MQ=255)                                                                                                                                                                                                  gATAAATAGTGCCATTCATCACAGATTCCCCAGATGGAATGTAATGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTc                       <  2:214720/251‑1 (MQ=255)                                                                                                                                                                                                      aaTAGTGCCATTCATCACCGATTCTCCAGAGGTAATGTAAGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGCCGCTGTCGCGTCTTATCAGACCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATCGCCTGCTTCTCTTCAagc                   >  2:228379/1‑251 (MQ=255)                                                                                                                                                                                                                       caGATTCTCCAGAGGTAATGTAAGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGCTGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGcc  >  2:10268/1‑251 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                     ACTCCAACACCAATTGGAATACCGCGTGTGACGCTGGTGTTCGGATTCAGCGGCGTGCCCAGCCAGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCC  >  NC_000913/4285002‑4285466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑