Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,284,646 G→A A86V (GCC→GTC)  actP ← acetate/glycolate:cation symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,284,6460GA97.6% 123.1 / NA 41A86V (GCC→GTC) actPacetate/glycolate:cation symporter
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (23/17);  total (24/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTC  >  NC_000913/4284400‑4284888
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  
cccACCATCTGGGCGATAAGTTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAAATCGAAACGGCCCTTTTTGCAGACGGTAAGAGGCCACATCGGCAAAGGTGTCGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCATCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGa                                                                                                                                                                                                                                                <  2:257515/251‑1 (MQ=255)
              gATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCgg                                                                                                                                                                                                                                  <  1:265438/251‑1 (MQ=255)
              gATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTAAGAAGGTAAGAGGCCACCTCGGCAAAGGTGTAGCGCACCCGGTTACGCAGACGTTCGGCAATCAGGACCAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGCGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCACgg                                                                                                                                                                                                                                  >  1:167458/1‑251 (MQ=255)
                      aaaGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGcc                                                                                                                                                                                                                          >  1:10724/1‑251 (MQ=255)
                             caccaccaccaGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGt                                                                                                                                                                                                                   >  1:132002/1‑251 (MQ=255)
                                cacccccaGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCt                                                                                                                                                                                                                <  2:232540/251‑1 (MQ=255)
                                caccaccaGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCt                                                                                                                                                                                                                >  1:215189/1‑251 (MQ=255)
                                  ccaccaGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTACGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGCAGCCCAGCGCGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTATgg                                                                                                                                                                                                              >  2:104411/1‑251 (MQ=255)
                                   caccaGAGAACCAAAGGCCGAAAGAATACGAATCGGCACTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGACGCCCAGCGAGTAAATTAAGACGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAAACCCCGGCAATCGCCAGCCCGTTCTgg                                                                                                                                                                                                              >  2:194317/1‑250 (MQ=255)
                                         agaACCACAGGCCGAAAGAATACGAAGCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTGCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAg                                                                                                                                                                                                       <  1:83411/251‑1 (MQ=255)
                                                 aGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTg                                                                                                                                                                                               <  2:10724/251‑1 (MQ=255)
                                                       aaaGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCt                                                                                                                                                                                         <  1:267546/251‑1 (MQ=255)
                                                                 aaTCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTgtagt                                                                                                                                                                               >  1:114397/1‑251 (MQ=255)
                                                                  aTCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTgtagt                                                                                                                                                                               >  1:210593/1‑250 (MQ=255)
                                                                            gTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACg                                                                                                                                                                     >  1:251276/1‑250 (MQ=255)
                                                                                        aaGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGACCGTACGCGt                                                                                                                                                        >  1:221371/1‑251 (MQ=255)
                                                                                               cacaTCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGCAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATg                                                                                                                                                 >  2:9796/1‑251 (MQ=255)
                                                                                                 aaTCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGcc                                                                                                                                               <  1:101340/250‑1 (MQ=255)
                                                                                                         aaGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGt                                                                                                                                       <  1:139614/251‑1 (MQ=255)
                                                                                                            gtgtAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATCTAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCCGTAGGTAAt                                                                                                                                    >  1:52760/1‑251 (MQ=255)
                                                                                                                aGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGcc                                                                                                                                 <  1:18081/250‑1 (MQ=255)
                                                                                                                    ccccAGGTTACGCAGACGTTCGGAAATCAGGAACAAAATGCTCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGAAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCCGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTTATGCCCAGTAGGTAATGCCGAGCg                                                                                                                            >  2:183714/1‑251 (MQ=255)
                                                                                                                      ccAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGt                                                                                                                           >  1:59973/1‑250 (MQ=255)
                                                                                                                        aGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTaaa                                                                                                                        <  2:22477/251‑1 (MQ=255)
                                                                                                                                                  gAACAAAAGTATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGGCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAAGCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACAtaataa                                                                                              <  2:221371/251‑1 (MQ=255)
                                                                                                                                                    aCAAAATGATCGGCCAGACCACCAGGAAGCCCAGCGAGTAACTTAAGCCGTAATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCACTCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGGGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCCGGAGGTAATGCCGCGAGTAAACACGACGAAAATCAGGAACATAATAATc                                                                                            >  2:16915/1‑251 (MQ=255)
                                                                                                                                                          tGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGCGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGc                                                                                      >  1:261966/1‑251 (MQ=255)
                                                                                                                                                              cGGCCAGCCCACCAGGAAGCCCAGCGAGTACATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGCGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGt                                                                                  >  1:75926/1‑251 (MQ=255)
                                                                                                                                                                 caaGCCCACCAGGAAGCTCAGCGAGTAAATTAAGCCGTTATAGCCGGAGGTAAACACCAGCTCGGAGATCCCCAAGAATGAGGCGACGGCCATATAGTCCCCGGCAAGCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTTATGCCCATGATGTAATGCCGAGCGTATACGCCCCGAAAATCAGGAACATAATAAGCGCCTACCAGTTc                                                                                <  2:161053/248‑1 (MQ=255)
                                                                                                                                                                          caGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTtggctggc                                                                      <  2:266317/251‑1 (MQ=255)
                                                                                                                                                                                       gAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGcccc                                                         <  2:132002/251‑1 (MQ=255)
                                                                                                                                                                                       gAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGcccc                                                         >  2:93421/1‑251 (MQ=255)
                                                                                                                                                                                          tAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGCATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGGACGCGTTTTGATGCCCAGTAGGGAATGCCGAGCGTAAACACGACGAAAATCAGGAACCTAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCt                                                      >  1:36295/1‑251 (MQ=255)
                                                                                                                                                                                           aaaTTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCTCTCTACGGCCCCGCt                                                      <  1:223119/250‑1 (MQ=255)
                                                                                                                                                                                               tAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGAAATAGCCAGCCCGTTATGGCAGCCAGTGATATTGCCGCCTGCGGTGTCGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCAAGTAGGTAATGCCGAGCGTAACCACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAAt                                                   >  1:175776/1‑249 (MQ=255)
                                                                                                                                                                                                              ggAGGTAAACCCCAGCGCGGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCTGCGTTAg                                  <  2:112060/251‑1 (MQ=255)
                                                                                                                                                                                                                         acAGCGCGGAGATCCCCCAGAATGAGGCGACGGACACATACTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCAGCCTGCGGTGTCGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCCGTAGGTACTGCCGAGCGTAACCACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTCATAGCATCCGCGGCGTTAGCTGCGAAAggg                       >  2:13283/2‑251 (MQ=255)
                                                                                                                                                                                                                               cgGAGATCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGtgtg                 >  1:202594/1‑251 (MQ=255)
                                                                                                                                                                                                                                  agaTCCCCAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTggcg              >  2:92606/1‑251 (MQ=255)
                                                                                                                                                                                                                                        ccAAGAATGAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAgc        <  1:148850/251‑1 (MQ=255)
                                                                                                                                                                                                                                                gAGGCGACGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTc  >  1:4263/1‑249 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  
CCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTC  >  NC_000913/4284400‑4284888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑