Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,283,465	G→A	P480S (CCG→TCG)	actP ←	acetate/glycolate:cation symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,283,465	0	G	A	100.0%	69.2 / NA	26	P480S (CCG→TCG)	actP	acetate/glycolate:cation symporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/8);  total (18/8)

AGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTT  >  NC_000913/4283230‑4283710                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                      aGACGACCGGGGACGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGatcatc                                                                                                                                                                                                                                          >  2:185193/1‑249 (MQ=255)                  gagagaTGAATGCGCGCTGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTTCGCGCGGAACAGTTCACGCTCACGCGCGCCGTCCGCGGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCa                                                                                                                                                                                                                       <  2:254302/251‑1 (MQ=255)                                                   aaaGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCACACGCGCGCCTTCCGCTGCGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCACAGCCAGCCACCCATCATCGCGCCAcgcg                                                                                                                                                                                      >  2:41867/1‑251 (MQ=255)                                                       ccGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTactact                                                                                                                                                                                                                                 <  2:55093/204‑1 (MQ=255)                                                       ccGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTactact                                                                                                                                                                                                                                 >  1:55093/1‑204 (MQ=255)                                                                 ggCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCTGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGAc                                                                                                                                                                        <  2:116912/250‑1 (MQ=255)                                                                                      aCAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACAAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGAATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCAATCATCGAGCCACGCGTGGTCAGTTTCGACCAGTACATAAAAAGCAGAATg                                                                                                                                                   >  2:181381/1‑251 (MQ=255)                                                                                          ttCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATAAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCg                                                                                                                                               >  1:25453/1‑251 (MQ=255)                                                                                                                 cTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCACGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCCTCATCGCGCCACGCGTGGACGGTTCCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATg                                                                                                                        >  2:149506/1‑251 (MQ=255)                                                                                                                                 cGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGcccc                                                                                                         >  1:93098/1‑250 (MQ=255)                                                                                                                                                       aaTGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGAtt                                                                                  >  1:92434/1‑251 (MQ=255)                                                                                                                                                             ccGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCAACAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCaaa                                                                            <  2:93098/250‑1 (MQ=255)                                                                                                                                                             aCGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTCTTCGTGACCAAGGATCTGTACCCAAATCGGCGAGCCGAGAACCATCAGTAACACTCCGGAAATAAGCACCAGCCAACCACCCCACAACGAGACACGCGTCGACAGTTTCGACCAGAACATAGAAAGCAGAATGAAAGGGAAAAAACCGCTCGCCCCGATGGCAAACGCCAGCCCAACCATAAAGGAGAtcttatctaacaccg                                                                             >  1:250327/1‑236 (MQ=255)                                                                                                                                                                         aaCAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAg                                                                <  1:185193/251‑1 (MQ=255)                                                                                                                                                                                   ggcaTTCATACGGGAAGATAGATTTTTCGTGACCAAGGATCTGAACCCAAATCGTCGAGCCGCGGATCATCAGTACTACTGCGGTACTCAGCCCCAGCCAGCCACCCATCATCGCGCCAAGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCAACATAAAGGCGATGTTCTGCTTCTCAAACAGCAAGCCGAGGATAATCGc                                                       >  2:107787/4‑250 (MQ=255)                                                                                                                                                                                          aTACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGc                                               >  1:50648/1‑251 (MQ=255)                                                                                                                                                                                                      ggCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGACGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGACGAGGATAATCGCAATAACGCCGAGGATCAga                                    >  1:55690/1‑249 (MQ=255)                                                                                                                                                                                                         tttttCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACg                                 >  1:71391/1‑250 (MQ=255)                                                                                                                                                                                                         tttttCGTGACCAAGGATCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACg                                 <  2:71391/250‑1 (MQ=255)                                                                                                                                                                                                                        gATCTGTACCCAAATCTTCGAGCCGAGGATCATCAGTACTGCTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCCGTACATAGAAAGCAGAATGATCGGGAAGCTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCAGCGGATCAGTAAGGTGATTTTTGATAccc                 >  2:90132/1‑251 (MQ=255)                                                                                                                                                                                                                         aTCTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGACAGTTTCGCCCAGTACATAAAAAGCAGCATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCACCACCATAAAGGCGATGTTCTGATTCTAAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGAACAGTACGGGGCTTTTTGATACCCg                >  1:12562/1‑251 (MQ=255)                                                                                                                                                                                                                           cTGTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGCCCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGATCGCCGCGATGGCAAAAGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGCTCAGTACGGAGCTTTTTGATACTCGc               >  1:250188/1‑250 (MQ=255)                                                                                                                                                                                                                             gTACCCAAATCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCATTACGGTGATTTTTGATACCCGCa              <  2:245172/249‑1 (MQ=255)                                                                                                                                                                                                                                 ccAAATCGCCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCACCAGCCAGCCCCCCATCAACGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTGACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTGCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTATTGATCCCCGCAGCTCtt        <  2:184703/251‑1 (MQ=255)                                                                                                                                                                                                                                    aaTCGTCGAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCCGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCAACCATAAAGGCGCTGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCCCGCAGAGGATCAGAACGGTGATTTTTGATACCCGCAGCTCttaac     >  1:118641/1‑248 (MQ=255)                                                                                                                                                                                                                                       cgtcgAGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCCGAACGGGGATTTTTGATACCCGCAGCTCTTCACGtt  >  2:258422/1‑251 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                      AGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCATCGCGCCACGCGTGGTCAGTTTCGACCAGTACATAGAAAGCAGAATGATCGGGAAGTTACAGCTCGCCGCGATGGCAAACGCCAGCCCCACCATAAAGGCGATGTTCTGATTCTCAAACAGCACGCCGAGGATAATCGCAATCACGCCGAGGATCAGTACGGTGATTTTTGATACCCGCAGCTCTTCACGTT  >  NC_000913/4283230‑4283710

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑