Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,281,093	G→A	A372T (GCG→ACG)	yjcE →	putative transporter YjcE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,281,093	0	G	A	97.7%	133.8 / NA	44	A372T (GCG→ACG)	yjcE	putative transporter YjcE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (16/27);  minor base T (0/1);  total (16/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGCTGCCGGGTATTCTGGAGACGTCGCTGATGGCAGCAGAAATCGACCCTAACGTCGAAATCTGGATGCTGTTTACCAATATTATTCTGATATATGCGGCGCTGATGCTGGTCCGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGGCGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCC  >  NC_000913/4280856‑4281330                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                              cAGCTGCCGGGTATTCTGGAGACGTCGCTGATGGCAGCAGAAATCGACCCTAACGTCGAAATCTGGATGCTGTTTACCAATATTATTCTGATATATGCGGCGCTGATGCTGGTCCGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGc                                                                                                                                                                                                                                  <  1:98863/251‑1 (MQ=255)      gCCGGGTATTCTGGAGACGTCGCTGATGGCAGCAGAAATCGACCCTAACGTCGAAATCTGGATGCTGTTTACCAATATTATTCTGATATATGCGGCGCTGATGCTGGTCCGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGCTCACTCTGGCCGgtg                                                                                                                                                                                                                             >  2:269128/1‑251 (MQ=255)          ggTATTCTGGAGACGTCGCTGATGGCAGCAGAAATCGACCCTAACGTCGAAATCTGGATGCTGTTTACCAATATTATTCTGATATATGCGGCGCTGATGCTGGTCCGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGct                                                                                                                                                                                                                         <  1:72907/251‑1 (MQ=255)                 tGGAGACGTCTCTGATGGCAGCAGAAATCGACCCTAACGTCGAAATCTGGATGCTGTTTACCAATATTATTCTGATATATGCGTCGCTGATGCTGGTCCGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTTGACCACACGAGAAATCCTGGTCGCGTCTTTCGCCGGGTTGCGTGGGACGATCACTCTGGCCGGTGTGTTTTCTAt                                                                                                                                                                                                                   <  2:108688/250‑1 (MQ=255)                        gTCGCTGATGGCAGCAGAAATCGACCCTAACGTCGAAATCTGGATGCTGTTTACCAATATTATTCTGATATATGCGGCGCTGATGCTGGTCCGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCgctgc                                                                                                                                                                                                           <  1:96384/251‑1 (MQ=255)                                     gcagAAATCGACCCTAACGTCGAAATCTGGATGCTGTTTACCAATATTATTCTGATATATGCGGCGCTGATGCTGGTCCGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATgg                                                                                                                                                                                              <  2:171326/251‑1 (MQ=255)                                           aTCGACCCTAACGTCGAAATCTGGATGCTGTTTACCAATAGTATTCTGATATATGCGTCGCTGATGCTGGTCCGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCTTTTCCTGAAGAAGAAGCCGAGGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGt                                                                                                                                                                                        <  1:123542/251‑1 (MQ=255)                                               aCCCTAACGTCGAAATCTGGATGCTGTTTACCAATATTATTCTGATATATGCGGCGCTGATGCTGGTCCGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTc                                                                                                                                                                                    >  1:51226/1‑251 (MQ=255)                                                cccTAACGTCGAAATCTGGATGCTGTTTACCAATATTATTCTGATATATGCGGCGCTGATGCTGGTCCGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTcc                                                                                                                                                                                   <  1:112600/251‑1 (MQ=255)                                                  cTAACGTCGAAATCTGGATGCTGTTTACCAATATTATTCTGATATATGCGGCGCTGATGCTGGTCCGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTACCGTCTTCCCg                                                                                                                                                                                 >  1:59084/1‑251 (MQ=255)                                                       gTCGACATCTGGATGCTGTTTACCAATATTATTCTGATATATGCGGCGCTGATGCTGGTCCGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGgcgcg                                                                                                                                                                            >  1:215223/1‑251 (MQ=255)                                                               cTGGATGCTGTTTACCAATATTATTCTGATATATGCGGCGCTGATGCTGGTCCGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGc                                                                                                                                                                    >  1:245573/1‑251 (MQ=255)                                                                  gatgctgtttaaacagattaatCTGATATATGCGGCGCTGATGCTGGTCCGTTTCGGCTAGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGATTGGTTCGTGGACCAAACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATAACTCTGGCCGGCGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTgg                                                                                                                                                                 >  2:194794/17‑251 (MQ=255)                                                                   aTGCTGTTTACCAATATTATTCTGATATATGCGGCGCTGATGCTGGTCCGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCATCCGCTTCCCGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGAGCACACGGGAAATCCTGATCGCGTCTTTCGCCGGGGGGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGgt                                                                                                                                                                <  1:27287/251‑1 (MQ=255)                                                                           tACCAATATTATTCTGATATATGCGGCGCTGATGCTGGTCCGTTGCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTTGTTCGTGGACCACACGAGAAATCCTGTTCGCGTCGTTCGCCGGGGTGCGTGGTACTATCACTCGGGCCGGTGTGTTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTg                                                                                                                                                         <  1:265963/250‑1 (MQ=255)                                                                              cAATATTATTCTGATATATGCGGCGCTGATGCTGGTCCGTTTCGGCTGGCTGTGGACGATGACAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACCCGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGGGTGCTCTCTATCCCGCTTCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGGTCCTggcgg                                                                                                                                                     >  2:242851/1‑251 (MQ=255)                                                                                     attCTGATATATGCGGCGCTGCTGCTGGTACGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGt                                                                                                                                              >  1:222870/1‑251 (MQ=255)                                                                                        cTGATATATGCGGCGCTGATGCTGGTCCGTTTCGGCTGGCTGTGGACGATGACAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGACGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTCTGAGCTGGTGTTCCTGGCGGCTGGCGTCAt                                                                                                                                           >  1:29517/1‑251 (MQ=255)                                                                                          gATATATGCGGCGCTGATGATGGTACGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGATTCCTGAAGAAGCAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGCGTTCCTGGCGGCTGGCggccttc                                                                                                                                         >  2:34604/1‑245 (MQ=255)                                                                                                   ggcgCTGATGCTGGTCCGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCgg                                                                                                                                                                                >  2:109068/1‑203 (MQ=255)                                                                                                   ggcgCTGATGCTGGTCCGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCgg                                                                                                                                                                                <  1:109068/203‑1 (MQ=255)                                                                                                         gATGCTGGTCCGTTTCGGCTGGTTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGTGACGATCACTCTGGCCGGTGTGCTCTCTATCCCTCTGCTCTTGCCGGATGGTAACGTCTTCCCGGTGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGtt                                                                                                                            <  1:246278/249‑1 (MQ=255)                                                                                                                       tCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGAt                                                                                                             <  1:269128/250‑1 (MQ=255)                                                                                                                         gggtggcggTGGACGATGAAAAATTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCGTCTCTCTGTGTGTCGGCGTGGGGATGtt                                                                                                          <  2:103648/248‑1 (MQ=255)                                                                                                                         ggctggctGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGGGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGGTCCTGGCGGCGATCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGtt                                                                                                          <  2:131331/251‑1 (MQ=255)                                                                                                                                   ggACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGGAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGAGCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTg                                                                                                <  2:51283/251‑1 (MQ=255)                                                                                                                                      cGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGTAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCTCTCTGGCCTGTGTGCTCTCTATCCCGCTTCTCTTGCCGGATGGTAACTTCTTCTCGGCGCGCTATGAGCTGGTGTTCCTGGCGTCTGTCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTa                                                                                             <  1:173942/251‑1 (MQ=255)                                                                                                                                        aTGAAAAAGATCAGCAACCGCTTCCTGAAGACGAAGCCGATGGAGCTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCtcc                                                                                            >  1:225782/1‑248 (MQ=255)                                                                                                                                             aaaGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAacac                                                                                       >  1:9333/1‑250 (MQ=255)                                                                                                                                                                  gaagaagaaGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGc                                                                 <  1:251232/251‑1 (MQ=255)                                                                                                                                                                     aaagaagCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCTTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGcaac                                                              <  2:245573/250‑1 (MQ=255)                                                                                                                                                                        gaagCCGATGGAGTTTGGTTCGTGGATCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGTTGTTTGTCTGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAAt                                                           <  2:136992/251‑1 (MQ=255)                                                                                                                                                                          agCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATTGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTg                                                         <  1:185448/251‑1 (MQ=255)                                                                                                                                                                                        ggTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCACTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTCCAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTAt                                           >  2:96435/1‑251 (MQ=255)                                                                                                                                                                                        ggTTCGTGGACCACACGAGAAAACCTGATCGCGTATGTCTCCGGGGTGCGCGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTGTTGCCGGATGGTAACGTCTGCCCGGCGAGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTGTCTTCTCGCAGTTTGGCGGCGTGGTGATGTTGCCTATTCGGCTACAACACATTGAAGGCGCGGATGATTCGCAACAAATGAAAGAGCAACGTAt                                           <  2:29517/251‑1 (MQ=255)                                                                                                                                                                                         gttaggtgACCACACGAGAAATCCTGTTCGTGTCGTGCGCCGGGGTGCGTGGGTCGATCGCTCTGGTCGGTGTGCTCTCTATCCCGTTGCTCGTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTTGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTAt                                           <  1:46179/243‑1 (MQ=255)                                                                                                                                                                                              tGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGAAACAATTGAAAGAGGAACGTATTGcgcg                                     >  1:36904/1‑251 (MQ=255)                                                                                                                                                                                                    acacGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGc                               >  1:100156/1‑251 (MQ=255)                                                                                                                                                                                                               ccTGATCGCGTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGaa                     <  1:104842/250‑1 (MQ=255)                                                                                                                                                                                                                   aTCGCTTCTTTCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATGGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGc                <  2:215223/251‑1 (MQ=255)                                                                                                                                                                                                                            ttCGCCGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATtgtg         <  1:35624/249‑1 (MQ=255)                                                                                                                                                                                                                            ggcGCCGGGGTGCGTGGGACGATCACTATGGCCGGGGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGACTTCCCGGCGCGCGATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCAGTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTgg        <  2:46614/248‑1 (MQ=255)                                                                                                                                                                                                                                ccGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCAGTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATc   <  1:66640/251‑1 (MQ=255)                                                                                                                                                                                                                                 cGGGGTGCGTGGGACGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATcc  <  2:74881/251‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                              CAGCTGCCGGGTATTCTGGAGACGTCGCTGATGGCAGCAGAAATCGACCCTAACGTCGAAATCTGGATGCTGTTTACCAATATTATTCTGATATATGCGGCGCTGATGCTGGTCCGTTTCGGCTGGCTGTGGACGATGAAAAAGTTCAGCAACCGCTTCCTGAAGAAGAAGCCGATGGAGTTTGGTTCGTGGACCACACGAGAAATCCTGATCGCGTCTTTCGCCGGGGTGCGTGGGGCGATCACTCTGGCCGGTGTGCTCTCTATCCCGCTGCTCTTGCCGGATGGTAACGTCTTCCCGGCGCGCTATGAGCTGGTGTTCCTGGCGGCTGGCGTCATTCTCTTCTCGCTGTTTGTCGGCGTGGTGATGTTGCCTATTCTGCTACAACACATTGAAGTCGCGGATCATTCGCAACAATTGAAAGAGGAACGTATTGCGCGAGCGGCAACGGCAGAAGTGGCGATTGTGGCGATCC  >  NC_000913/4280856‑4281330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑