Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,276,422	C→T	P318S (CCG→TCG)	pdeC →	c‑di‑GMP phosphodiesterase PdeC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,276,422	0	C	T	93.8%	44.7 / NA	16	P318S (CCG→TCG)	pdeC	c‑di‑GMP phosphodiesterase PdeC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (12/3);  minor base G (0/1);  total (12/4)
Fisher's exact test for biased strand distribution p-value = 2.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

CCGGTATTTTGGGGAGTCTTGTTCTGCTATTACTCTGGCTACGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACGTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTCGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAATTTGAAGTGACTGAGCATGC  >  NC_000913/4276216‑4276663                                                                                                                                                                                                               |                                                                                                                                                                                                                                                  acgGTATGTTGGTGAGTCTTGTTCAGCTATTACTCTGGCTACGTAGTCGCAAAAACTATTGATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTGTGTCTTGATTACCAGCCAATAATCGTTATCAAAACAGAAAAATGTATCGGAGCTGAAGCGTTGTTACGTTGGCCTGGTGAGCAGGGGAAAATAATGACTGCGGCAGAGTTTATTCCGCCGGCAGAAAAGGAGGGGATGATAGaa                                                                                                                                                                                                       <  2:127525/250‑1 (MQ=255)                        cttcTATTCCTCTGACTACGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACGTTGGCCTGGTGAGCAGGGGCAAATAATGAATTCGGCAGAGTTTATTCCGCTGGCAGAAAAGAAGGGGATGATAGAACAGATAACTGATTATGTTAtgg                                                                                                                                                                                 >  2:150098/4‑248 (MQ=255)                                       cTACGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACGTTGGCCTGGTGAGCAGGGGCAAATAATGAATTCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCcgcg                                                                                                                                                                  >  1:28704/1‑250 (MQ=255)                                         aCGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACGTTGGCCTGGTGAGAAGGGGCAAATAATGAATTCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATc                                                                                                                                                               >  2:34297/1‑251 (MQ=255)                                           gTATTCGACAAAACTAATTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAAAATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGCATCGGCGCTGAAGCGTTGTTACGTTGGCCTGGTGCGCAGGGGCAAATAATGAATTCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTg                                                                                                                                                             >  2:72311/1‑251 (MQ=255)                                                     aaaCTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACAGCAACTTTGTCTGTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTAGCGGCGCTGAAGCGGTGTTACGTTGGCCTGGTGAGCAGGGGCAAATAATGAATTCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTAcc                                                                                                                                                   <  1:240743/251‑1 (MQ=255)                                                                         gTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACGTTGGCCTGGTGAGCAGGGGCAAATAATGAATTCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATc                                                                                                                                 >  1:82577/1‑249 (MQ=255)                                                                         gTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGAGCTGAAGCGTTGTTACGTTGGCCTGGTGAGCAGGGGCAAATAATGAATTCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATc                                                                                                                                 <  2:82577/249‑1 (MQ=255)                                                                                          cTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACGTTGGCCTGGTGAGCAGGGGCAAATAATGAATTCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCt                                                                                                              >  1:160854/1‑251 (MQ=255)                                                                                              aaaaaCATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACGTTGGCCTGGTGAGCAGGGGCAAATAATGAATTCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTCg                                                                                                          >  1:262127/1‑251 (MQ=255)                                                                                                                            cAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACGTTGGCCTGGTGAGCAGGGGCAAATAATGAATTCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTCGGCCTCCGATTTTCATACGTCACGGTTGAt                                                                             >  2:50871/1‑250 (MQ=255)                                                                                                                                             aCAGAAAAATGTATCGGCGCTGAAGCGTTGTTACGTTGGCCTGGTGAGCAGGGGCAAATAATGAATTCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTCGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGa                                                            >  1:83410/1‑250 (MQ=255)                                                                                                                                             aCAGAAAAATGTATCGGCGCTGAAGCGTTGTTACGTTGGCCTGGAGAGAAGGGGCAAATAATGAATTCGGCAGAGTTTATTCCGCAGGCAGACAAGGAGGGGATGATAGAAAAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTCGGCCTCCGATTTTCATACGTCACGTGTGATAGCGCGAATCAATCAGa                                                            >  2:191107/1‑250 (MQ=255)                                                                                                                                                                cTGAAGCGTTGTTACGTTGGCCTGGTGAGCAGGGGCAAATAATGAATTCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTCGGCCTCCGATTTTAATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTg                                        >  1:122764/1‑251 (MQ=255)                                                                                                                                                                           ttACGTTGGCCTGGTGAGCAGGGGCAAATAATGAATTCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTCGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCgcagc                              >  1:126420/1‑250 (MQ=255)                                                                                                                                                                                                      agaatGAATTCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAGCTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTCTCTACTATCCTGTCGGCCGCCGATTTTCATACGTCTCGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAGTACGCGGTGCGTTCGCAGCAAATTAAATTTGAAGTGACTGAgcatgc  <  1:17220/249‑1 (MQ=255)                                                                                                                                                                                                               |                                                                                                                                                                                                                                                  CCGGTATTTTGGGGAGTCTTGTTCTGCTATTACTCTGGCTACGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGTCTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACGTTGGCCTGGTGAGCAGGGGCAAATAATGAATCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTCGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAATTTGAAGTGACTGAGCATGC  >  NC_000913/4276216‑4276663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑