Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,276,212	C→T	P248S (CCC→TCC)	pdeC →	c‑di‑GMP phosphodiesterase PdeC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,276,212	0	C	T	100.0%	25.3 / NA	10	P248S (CCC→TCC)	pdeC	c‑di‑GMP phosphodiesterase PdeC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/5);  total (5/5)

GGGTGTGTATGATACGGTGACGAAAACCTTTTTCTCGTTAAGCAAAGAGGCCTCGGCAGCAACGTTTTCTCCGCTGATTCATTTGAAGGATTTAACCGTACAAAGAAATGGCTATTTATATGCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATATCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGCCCGCCGGTATTTTGGGGAGTCTTGTTCTGCTATTACTCTGGCTACGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGT‑CTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACGTTGGCCTGGT  >  NC_000913/4275992‑4276400                                                                                                                                                                                                                             |                                                                                                                                                                                              gggTGTGCATGCTACGGTGACGACAACCTTTTTCTCGTTAAGCAAAGAGGCCTCGGCAGCAACGTTTTCTCCGCTGATTCATTTGAAGGATTTAACCGTACAAAGAAATGGCTATTTATATGCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATATCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGTCCGCCGGTATTTTGGGGAGTCTTGTTCTGc                                                                                                                                                                 >  2:195718/1‑251 (MQ=255)             tACGGTGACGAAAACCTTTTTCTCGTTAAGCAAAGAGGCCTCGGCAGCAACGTTTTCTCCGCTGATTCATTTGAAGGATTTAACCGTACAAAGAAATGGCTATTTATATGCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATATCAACGTCTTATCACCCATTTTTATAATCATCTTATTTTTGCGTTGTCCGCCGGTATTTTGGGGAGTCTTGTTCTGCTATTACTCTGGc                                                                                                                                                     >  2:233460/1‑251 (MQ=255)                ggTGACGAAAACCTTTTTCTCATTAAGCATAGAGGCCTCGGCCGCAACGTTTTCTCCGCTGATTCATTTGAAGGCTTTATCCGTACACAGAAATGGCTATTTATATGCGCCAGTCTATTCGCCAACACGCCCACTTGCAGCCATTGTTGCGACTTCATATCACCGTCTTATAACCCATTTTTATACTCATCTTATTTTTGCGTTGTCCGACGTTATTTTGGGGTGTCTTGTTCTGCTTTTACTCTGGCTAc                                                                                                                                                  >  2:240743/1‑251 (MQ=255)                                 tctcGTTAAGCAAAGAGGCCTCGGCAGCAACTTTTTTGCCGCTGATTCATTTGAAGGATTTAACCGTACAAAGAAAGGGCTATTTATATTCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATATCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTGGTCCGCCGGTATTTTGGGGAGTCTTGTTCTGCTATTACTCTGGCTACTTATACGACAAAACTa                                                                                                                                  <  2:196501/250‑1 (MQ=255)                                        aaGCAAAGAGGCCTCGGCAGCAACGTTTTCTCCGCTGATTCATTTGAAGGATTTAACCGTACAAAGAAATGGCTATTTATATGCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATATCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGTCCGCCGGTATTTTGGGGAGTCTTGTTCTGCTATTACTCTGGCTACGTATTCGACAAAACTATTTAtctc                                                                                                                          <  1:52668/251‑1 (MQ=255)                                                       ggCAGCAACGTTTTCTCCGCTGATTCATTTGAAGGATTTAACCGTACAAAGAAATGGCTATTTATATGCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATATCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGTCCGCCGGTATTTTGGGGAGTCTTGTTCTGCTATTACTCTGGCTACGTATTCGACAAAACTATTTa                                                                                                                              <  1:100513/232‑1 (MQ=255)                                                       ggCAGCAACGTTTTCTCCGCTGATTCATTTGAAGGATTTAACCGTACAAAGAAATGGCTATTTATATGCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATATCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGTCCGCCGGTATTTTGGGGAGTCTTGTTCTGCTATTACTCTGGCTACGTATTCGACAAAACTATTTa                                                                                                                              >  2:100513/1‑232 (MQ=255)                                                                                                ccggaCAAAGAAAGTGCTATTTATATGCGACAATTTATGCGGCAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATATCAACGTCTTATAACCCATTTTTATAATCAGCTTATTTTTTCGTGGTCCTCCGGTATTTTGGGGAGTCTTGTGCTGCTATTACTCTGGCTACGTATCCGACAAAACTAGGTATCTCCCAAACTTAAATTGGAACTCGCCCTCGAAAAACGTCATCTT‑GTGCTTTACTACCAg                                                                 <  2:112149/247‑1 (MQ=255)                                                                                                                cTATTTATATGCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATATCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGTCCGCCGGTATTTTGGGGAGTCTTGTTCTGCTATTACTCTGGCTACGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGT‑CTTTATTACCAGCCAATAATCGATATc                                                  >  2:202002/1‑250 (MQ=255)                                                                                                                                                                tgttgCGACTTCATATCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGTCCGCCGGTATTTTGGGGAGTCTTGTTCTGCTATTACTCTGGCTACGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGT‑CTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACGTTGGCCTGGt  <  1:233460/250‑1 (MQ=255)                                                                                                                                                                                                                             |                                                                                                                                                                                              GGGTGTGTATGATACGGTGACGAAAACCTTTTTCTCGTTAAGCAAAGAGGCCTCGGCAGCAACGTTTTCTCCGCTGATTCATTTGAAGGATTTAACCGTACAAAGAAATGGCTATTTATATGCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATATCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGCCCGCCGGTATTTTGGGGAGTCTTGTTCTGCTATTACTCTGGCTACGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACGCGCCCTCGAAAAACATCAACTTTGT‑CTTTATTACCAGCCAATAATCGATATCAAAACAGAAAAATGTATCGGCGCTGAAGCGTTGTTACGTTGGCCTGGT  >  NC_000913/4275992‑4276400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑