Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,215,670	G→T	D65Y (GAT→TAT)	aceB →	malate synthase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,215,670	0	G	T	100.0%	58.8 / NA	19	D65Y (GAT→TAT)	aceB	malate synthase A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (8/11);  total (8/11)

TCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGC  >  NC_000913/4215431‑4215903                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                          tCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTTATTTTATTTc                                                                                                                                                                                                                                 <  1:215049/250‑1 (MQ=255)            ggggAAGCTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCATCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTTATTTTATTTCGGAAACAGCTTc                                                                                                                                                                                                                     <  1:98085/251‑1 (MQ=255)                 aGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGAGTGCGGAACGTTGCCTTATTTGATTTCGGAAACAGCGTGCATTc                                                                                                                                                                                                                <  1:210281/251‑1 (MQ=255)                                            cACGATGACTGAACAGGCAAAAACAACCGATGACCTGGCTTTCACAAGGCCGTATGGCGCGCAGGAGAAGCAAATTCTTACTGCAGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCggg                                                                                                                                                                                      >  2:268129/1‑250 (MQ=255)                                                                               tGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGAt                                                                                                                                                   >  2:19471/1‑250 (MQ=255)                                                                                    ttCACAAGGCCGTATGGCGAGCATGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCTACAACGCAATAAACTTCTGGAAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGTAACGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTg                                                                                                                                              <  1:142341/250‑1 (MQ=255)                                                                                               gTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAgc                                                                                                                                  <  1:19471/251‑1 (MQ=255)                                                                                                       aGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGCAGAATTTCTGACTGAGCTGGTGCCGCATTTTCCGCCACAACGCAATAAACTTCTGGCCGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGCTTGGAAAATTCGCGGGATTCCTGCGGCCTTAGAAGACCGCCGCGTAGAGATACCTGGACCGGTAAAGCGCAAgctg                                                                                                                          >  2:157364/1‑248 (MQ=255)                                                                                                                 gCAAATTCTTACTGCCGAAGCGGTAGAATTTTTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGCAGAGATAACTGGCCCGGTAGAGCGCAAGATGGGGATCAAcg                                                                                                                >  2:137588/1‑251 (MQ=255)                                                                                                                     aTTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCt                                                                                                            >  1:91341/1‑251 (MQ=255)                                                                                                                      ttCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCACCGCGCTc                                                                                                           >  1:97870/1‑251 (MQ=255)                                                                                                                                         aGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCt                                                                                        <  1:10382/251‑1 (MQ=255)                                                                                                                                                                       tACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGAGGGTGATCAACGCGCTCAACGCCCATGTGAAAGTCTTTATGGCCGATTTCGAAGATTGACTGGCAc                                                          >  2:12046/1‑251 (MQ=255)                                                                                                                                                                               aaCGCAATAAACTTCTGGCAGCGCGCATTCAGCATCAGCAAGATATTGATAACGGAACGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATGCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGTCACCAGACTgg                                                  <  1:134631/251‑1 (MQ=255)                                                                                                                                                                                   caagaaaCTTCTGTCAGGGCGCAGTCAGCAGCAGCAAGATATTGATATGTGAACGTTGCCTTAGTTTATTTCGGAAACAGGGTCCATTCGCGTTGCTGATTGGAAAATTCGCGGGATTCCTTCGGACTAAGAAGACCGCCGCGGAGAGTTAACTGGCCCGGTAGAGCGCAAGATGGTGATCAATGCTCTTAACGCCAATGTGATAGTCTTTATGGCTGATGTCGAAGATTCAGTGGCTCGTGACTGGaa                                                <  2:159490/245‑1 (MQ=255)                                                                                                                                                                                                       gcATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAAtt                          >  1:49239/1‑251 (MQ=255)                                                                                                                                                                                                                     caAGATATTGATAACGGAACGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGc            <  1:89580/251‑1 (MQ=255)                                                                                                                                                                                                                           atTGATAACGGAACGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGAGTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGAGTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTaa      <  1:24173/251‑1 (MQ=255)                                                                                                                                                                                                                               aTAACGGAACGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATGCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGAGCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGc  <  2:72388/251‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                          TCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGC  >  NC_000913/4215431‑4215903

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑