Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,133,252	C→T	P213S (CCG→TCG)	metF →	5,10‑methylenetetrahydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,133,252	0	C	T	100.0%	46.1 / NA	18	P213S (CCG→TCG)	metF	5,10‑methylenetetrahydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/10);  total (8/10)

GCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGG‑AAATTATT‑CCGGGAATTTTGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGC‑AAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACT‑GGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGATTTGCCATACGCTGGGGGTTCGACCT  >  NC_000913/4133063‑4133497                                                                                                                                                                                                |                                                                                                                                                                                                                                                        gcggcgTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCTGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGG‑AAATTATT‑CCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGc                                                                                                                                                                                            <  2:148309/251‑1 (MQ=255)                   cccccGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTTAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGG‑AAATTATT‑CCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTGGCCGATATGACCAACGTTCGTATTCCTGCGTGGTTGGCGC‑AAATGt                                                                                                                                                                          <  2:25971/248‑1 (MQ=255)                               aaaaGCGCTCCGGCGGATTTGATTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTTGTGTGG‑GTATTATTTCCGGGAATTT‑GTCGGTATTTAACTTTTAACAGGCGAGGAAATTTGTCGATATTACCAACGTGCGTATTTCGGCGTGGATGGCGCCAAATGtcagcgggcttg                                                                                                                                                               >  1:52669/1‑239 (MQ=255)                               aaaaGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGG‑AAATTATT‑CCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGC‑AAATGTTCGACGGTCTGga                                                                                                                                                             <  1:11753/251‑1 (MQ=255)                                     gcTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCCTTTATGTGGGAAATTATT‑CCGG‑AAATTTATCGGTATTTAATATTTAACAAGAGAAGAAATTTGCCGATATGACCAACGGGCGGATTCTCGCGTGGAATGCGC‑AAATGTTTGAAGGTCTGggtgatgg                                                                                                                                                       >  1:47158/1‑250 (MQ=255)                                        cAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGG‑AAATTATT‑CCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGC‑AAATGTTCGACGGTCTGGATGATGATGc                                                                                                                                                    <  1:145531/251‑1 (MQ=255)                                               aTTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGG‑AAATTATT‑CCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGC‑AAATGTTCGACGGTCTGGATGATGATGCCGAAAcc                                                                                                                                             <  1:62814/251‑1 (MQ=255)                                                            gAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGG‑AAATTATT‑CCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGC‑AAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACT‑GGtt                                                                                                                                <  1:129296/250‑1 (MQ=255)                                                                  cAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGG‑GAATTATT‑TCGGGGATTTTGTTGGTATTTAACTTTAAACAAGCGAAGAAATTTTCCGGTATTACCAAAGAGCGTATTCCGGCGGGGGTGGGGG‑AAATTTTTGACGGGCTGGGTGGTGATGGCGAACCCCGCAAACA‑GGTTGGagcgg                                                                                                                         >  2:204422/1‑250 (MQ=255)                                                                   aaaGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGG‑AAATTATT‑CCGGGGATTTTGTCGGGATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGGGGATGGCGC‑AAATGGTCGACGGGCTGGGTGATGATGCCGAAACCCGCAAACT‑GGGTGGcgcgg                                                                                                                         >  1:203488/1‑249 (MQ=255)                                                                                 aGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCCTTGATGTGG‑AAATTATT‑TCGGGGATTTTTTCGGTATCTAACTTTTAAAAGGCGAAGAAATTTTCCGCTATGACCAACGTGCGGATTCCCGCGTGGGTGGCGC‑AAATGTTCGGCGGGGTGGATGATGATGCCGCAAACCGCAAACttggttgggcggctatttccatgggt                                                                                                            >  1:68671/1‑232 (MQ=255)                                                                                                                     cGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGG‑AAATTATT‑CCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGC‑AAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACT‑GGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGAtt                                                                      <  1:267047/251‑1 (MQ=255)                                                                                                                                                      aTCGGCGGGCAGTGATGTGG‑AAATTATT‑CCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGC‑AAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACT‑GGTTGGCGCGAATATTGCCATTGATATGGTGAAGATTTTAATCCGTGAAGGTGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATg                                      <  1:19569/250‑1 (MQ=255)                                                                                                                                                           cggGCATTGATGTGG‑AAATTATT‑CCGGGAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATCTGACCAACGTGCGTATTCCGGAGTGGATGGCGC‑AAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACT‑GGCTGGCGCGAATATTGCCATGGATATGGTGATGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCAATACTATACGCTTACCCGTGCTGAAATGAGTTAc                                >  1:239646/1‑251 (MQ=255)                                                                                                                                                              gCATTGATGTGG‑ACATTATT‑CCGGGAATTTTGTCGGTGTCTAACTTTACACAGGCGAAGAACTTTGCCGATATGACCAACGTGCGTATTACGGCGTGGATGGCGC‑AAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACT‑GGTTGGCGCGAATATTGCCTTGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGACAGATTTCCACTTCTCTACGCTTAACCGTGCTGAAATGAGTTAcgcg                             >  2:266204/1‑251 (MQ=255)                                                                                                                                                                                      gggAATTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGC‑AAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACT‑GGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGATTTGCCATACGCTGGGGGTTc       <  1:138720/251‑1 (MQ=255)                                                                                                                                                                                          aTTTTGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGC‑AAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACT‑GGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGATTTGCCATACGCTGGGGGTTCGAcc   >  2:202531/1‑251 (MQ=255)                                                                                                                                                                                            tttGTCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGC‑AAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACT‑GGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGATTTGCCATACGCTGGGGGTTCGACCt  <  1:208870/250‑1 (MQ=255)                                                                                                                                                                                                |                                                                                                                                                                                                                                                        GCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGG‑AAATTATT‑CCGGGAATTTTGCCGGTATCTAACTTTAAACAGGCGAAGAAATTTGCCGATATGACCAACGTGCGTATTCCGGCGTGGATGGCGC‑AAATGTTCGACGGTCTGGATGATGATGCCGAAACCCGCAAACT‑GGTTGGCGCGAATATTGCCATGGATATGGTGAAGATTTTAAGCCGTGAAGGAGTGAAAGATTTCCACTTCTATACGCTTAACCGTGCTGAAATGAGTTACGCGATTTGCCATACGCTGGGGGTTCGACCT  >  NC_000913/4133063‑4133497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑