Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,967,770	C→T	intergenic (+94/‑146)	rho → / → rfe	transcription termination factor Rho/UDP‑N‑acetylglucosamine‑‑undecaprenyl‑phosphate N‑acetylglucosaminephosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,967,770	0	C	T	100.0%	24.4 / NA	11	intergenic (+94/‑146)	rho/rfe	transcription termination factor Rho/UDP‑N‑acetylglucosamine‑‑undecaprenyl‑phosphate N‑acetylglucosaminephosphotransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/7);  total (4/7)

GAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGCGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTATGCAATTTGCATATCAAATGGTTAATTTTTGCACAGGACTGGTGGGTTTGGAACGGACTTTCCCTTCTGAATAAAGGTCTTCGTGGTTATACTTCTGCTAATAATTTTCTCTGAGAGCATGCATTGTGAATTTACTGACAGTGAGTACTGATCTCATCAGTATTTTTTTATTCACGACACTGTTTCTGTTTTTTGCCC  >  NC_000913/3967542‑3967988                                                                                                                                                                                                                                     |                                                                                                                                                                                                                           gaaCTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGCGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCTTGGAATTTGCTGGCATGTTATg                                                                                                                                                                                                      <  1:157354/251‑1 (MQ=255)                        gcgcAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCAGTAATAAAATGGCAATGACCAAGACCAATGACGATTTATTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGCGTTTTGCTTTTATATCTGTAATCTGAAGGCCGCGCTGGCGATGTTAGGAAAATGCTTGGAATTTGCTGGCATGTTATGCAATTTGCAGATCAAATGGTTaa                                                                                                                                                                               <  2:24439/251‑1 (MQ=255)                                                            aaTGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATTATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACTTGGCGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCTTGGAATTTGCTGGCATGTTATGCAATTTGCATATCAAATGGTTAATTTTTGCACAGGACTGGTGGGTTTGGAACGGACttt                                                                                                                                           <  1:149828/251‑1 (MQ=255)                                                                 aaTTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGCGTTTTGCGTTTATATCTGTAAGCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCTTGGAATTTGCTGGCATGTTATGCAATTTGCATATCAAATGGTTAATTGTTGCACAGGACTGGTGGGTTTGGAACGGACTTTCCCtt                                                                                                                                      <  2:190114/251‑1 (MQ=255)                                                                     ccTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGCGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCTTGGAATTTGCTGGCATGTTATGCAATTTGCATATCAAATGGTTAATTTTTGCACAGGACTGGTGGGTTTGGAACGGACTTTCCCTTCTGa                                                                                                                                  <  1:245675/251‑1 (MQ=255)                                                                                                         cGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGCGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCTTGGAATTTGCTGGCATGTTATGCAATTTGCATATCAAATGGTTAATTTTTGCACAGGACTGGTGGGTTTGGAACGGACTTTCCCTTCTGAATAAAGGTCTTCGTGGTTATACTTCTGCTAATAAtt                                                                                              <  1:135445/251‑1 (MQ=255)                                                                                                                                                                    tttccGTGACGTTTTGATTTTATATCTGTAATCTTAAAGCCGCGCTGGCGATGTTATGAAAATTATTGGAATTTGCTGGAATGTTATGCACTTTGCATATAAAATGGTTAATTTTTGCACAGGACTGGTGGGTTTGGAACGGACTTTCCCTTCTGAATAAAGGTCTTCGTGGTTATACTTCTGCTAATAATTTTCTCTGAGAGCATGCATTGTGCATTTACTGACAGTGCGTACTGTTCTCTTCAGTCttt                                   >  2:89901/5‑251 (MQ=255)                                                                                                                                                                                  tGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCTTGGAATTTGCTGGCATGTTATGCAATTTGCATATCAAATGGTTAATTTTTGCACAGGACTGGTGGGTTTGGAACGGACTTTCCCTTCTGAATAAAGGTCTTCGTGGTTATACTTCTGCTAATCATTTTCTCTGAGAGCATGCATTGTGAATTTACTGACAGTGAGTACTGATCTCATCAGTATTTTTTTATTcccgcac                     >  1:115829/1‑245 (MQ=255)                                                                                                                                                                                       ttATATCTGTAATCTTAGTGCCGCGCTGGAGGTGTTCGGTAAATTCTTGGAATTTCCTGGAATGATATGCAATTTGCATATCAAATGGTTAATTTTTGCACAGGACTGGTGGGTTTGGAACGGACTTTCCCTTCTGAATAACGGTCTTCGTGGTTATACTTCTGCTAATAATTTTCTCTGAGAGCATGCATTGTGAATTTACTGACAGTGAGTACTGATATCATCAGAATTTTTTTATTCACGACActgtt                >  2:155183/1‑251 (MQ=255)                                                                                                                                                                                         atatTTGTAATCTTAATGCCGCTCTGTCGATGTTAGGAAAATTCTTGGAATTTGCTGGCATGTTATGCAAGTTGCATATTAAATGGTTAAGTTTTGCACAGTACTGGTGGGTTTGGAACGGACTTTCCCTTCTGAATAAAGATTTTCGTGGTTATTCTTCTGCTAATAATTTTCTCTGAGAGCATGCATTGTGAATTTACTGACAGTGAGTTCTGATCTCATCAGTATGTTGTTATTCCCGACActgtttc              <  2:8975/251‑1 (MQ=255)                                                                                                                                                                                                     ttAATGCCGCGCTGGCGATGTTAGGAAAATTCTTGGAATTTGCTGGCATGTTATGCAATTTGCATATCAAATGGTTAATTTTTGCACAGGACTGGTGGGTTTGGAACGGACTTTCCCTTCTGAATAAAGGTCTTCGTGGTTATACTTCTGCTAATAATTTTCTCTGAGAGCATGCATTGTGAATTTACTGACAGTGAGTACTGATCTCATCAGTATTTTTTTATTCACGACACTGTTTCTGTTTTTTGccc  >  1:44317/1‑251 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                           GAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGCGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTATGCAATTTGCATATCAAATGGTTAATTTTTGCACAGGACTGGTGGGTTTGGAACGGACTTTCCCTTCTGAATAAAGGTCTTCGTGGTTATACTTCTGCTAATAATTTTCTCTGAGAGCATGCATTGTGAATTTACTGACAGTGAGTACTGATCTCATCAGTATTTTTTTATTCACGACACTGTTTCTGTTTTTTGCCC  >  NC_000913/3967542‑3967988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑