Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,931,779	T→C	F155S (TTT→TCT)	kup →	K(+):H(+) symporter Kup

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,931,779	0	T	C	100.0%	59.6 / NA	20	F155S (TTT→TCT)	kup	K(+):H(+) symporter Kup
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/9);  total (11/9)

GTAATACGTCGGCGCGAACCACATCAATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGC  >  NC_000913/3931590‑3932024                                                                                                                                                                                              |                                                                                                                                                                                                                                                      gtcaTACGTCGGCGCGAACCACATCAATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGAcc                                                                                                                                                                                          >  2:78302/4‑251 (MQ=255)      aCGTCGGCGCGAATCACATCAATGCTGGTTATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCTGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGt                                                                                                                                                                                      <  1:130412/250‑1 (MQ=255)        gTCGGCGCGAACCACATCAATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAAAACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTATGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCCATTATCGTTCTCACTTTATTATCTATTCTTCAAAAACATGGCACGGCTATGGACGGTAAGATGTTTGCGACGAACATGCTAACCTGGtttt                                                                                                                                                                                   >  2:220113/1‑251 (MQ=255)                 aaCCACAGCAATGCTGGTGATTATGGGGCTAATCGGCGGCATCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGTATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTTGTTTTTGATTCTgg                                                                                                                                                                          <  1:39857/251‑1 (MQ=255)                                      tATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGAACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGc                                                                                                                                                      >  1:105544/1‑250 (MQ=255)                                              tAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGAt                                                                                                                                                                                                    >  2:7783/1‑196 (MQ=255)                                              tAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGAt                                                                                                                                                                                                    <  1:7783/196‑1 (MQ=255)                                                           cTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTTATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGtgc                                                                                                                                >  2:218715/1‑251 (MQ=255)                                                                   aTGGTGAATGCGTCATAACACCCTCTATTTCGGTTATGTCAGCTATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCGCGTCTATGGTCGGTAAGCGGTTTGCGCCGATCATTCTGACCTGGTTTTTGAGTCTGGCAGGGCTGGGCTTACGTAGCATTATTGCTATccctgtcctgctgcatg                                                                                                                          <  2:183198/249‑15 (MQ=255)                                                                                cATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTCTCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGGGCTGCATGCGCTGAATCCAAtgt                                                                                                           >  1:211006/1‑251 (MQ=255)                                                                                   aaCACACGCAATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGGTGGATACCTGGATCGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGCTTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGACGTGCTGCATGCGCTGAATCCAATGTggg                                                                                                        >  2:130255/1‑251 (MQ=255)                                                                                       cccGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGt                                                                                                    <  1:172510/251‑1 (MQ=255)                                                                                             aTTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCTGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCATGACTGGGGTTACGTAGCATTATGTCTATCCCTGAAGTGCTGCATGCGCTGAATCCAATTTGGGCGGTGCAtt                                                                                               <  2:17891/250‑1 (MQ=255)                                                                                                  ggTGATGTCAGCCATTGAAGGTCGGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGAGTCAAAAACATGGCACTGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGTAAGTGCTGCATGCTCTGAATCCAATGTGGGCGGTGCATTTCTTcc                                                                                         <  2:160552/251‑1 (MQ=255)                                                                                                   gTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCt                                                                                        >  2:134756/1‑251 (MQ=255)                                                                                                                          ggAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTGTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTGACCCGGAAGTGGTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACTAAACGGTTGCtttt                                                                  <  2:41306/250‑1 (MQ=255)                                                                                                                                             cTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTACGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTCGGGGCAgtggg                                              >  2:143753/1‑250 (MQ=255)                                                                                                                                                       ggATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGAtt                                    <  1:185988/251‑1 (MQ=255)                                                                                                                                                              tCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGAACGGCTATGGTCGGTAAGCTGTTTGCGCCGATAATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGACTCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCCTTAGGGGCAGTGGTGTTGTCGATTACggggg                             >  1:262506/1‑251 (MQ=255)                                                                                                                                                               ccccTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGGGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGGTGTCGATTACGGGGGt                            >  2:12307/1‑251 (MQ=255)                                                                                                                                                                                          ttatctatGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGCATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGc  >  1:172513/1‑250 (MQ=255)                                                                                                                                                                                              |                                                                                                                                                                                                                                                      GTAATACGTCGGCGCGAACCACATCAATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGC  >  NC_000913/3931590‑3932024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑