Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,834,564	G→A	Q456* (CAA→TAA)	yicJ ←	putative xyloside transporter YicJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,834,564	0	G	A	100.0%	62.7 / NA	21	Q456* (CAA→TAA)	yicJ	putative xyloside transporter YicJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/9);  total (12/9)

CTCAATCCGCACACCGACAATACCTTCCTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCACGGGGGGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTGAATCAAATTGAGGCCAGGTTGAATCAACCAGTTTCCATCGCTAATTTTCATTGCCGTTCCTTAGTTCTGCACTTCTTGAGAGGTGAATTGTTGCTGGCAACGGCGTTTACCCTGAGCCAGCTGTTCCATAACGGTTTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGATCGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCC  >  NC_000913/3834328‑3834808                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                     cTCAATCCGCACACCGACAATACCTTCCTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCACGGGGGGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTGAATCAAATTGAGGCCAGGTTGAATCAACCAGTTTCCATCGCTAATTTTCATTGCCGTTCCTTAGTTCTGCACTTCTTAAGAGGCGAAttgtt                                                                                                                                                                                                                                        >  1:76897/1‑251 (MQ=255)            cacCGACAATACCTTCCTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCACGGGGTGCAGCATAGACCACCATTGCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTGAATCAAATTGAGGCCAGGTTGAATCAACCAGTTTCCATCGCTAAGTTTCATTGCCGTTCCTTAGTTCTGCACTTCTTAAGAGGTGAATTGTTGCTGGCAACgg                                                                                                                                                                                                                             <  2:152692/251‑1 (MQ=255)                                ggggAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCACGGGGGGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTGAATCAAATTGAGGCCAGGTTGAATCAACCAGTTTCCATCGCTAATTTTCATTGCCGTTCCTTAGTTCTGCACTTCTTAAGAGGTGAATTGTTGCTGGCAACGGCGTTTACCCTGAGCCAGCt                                                                                                                                                                                                          <  2:152244/250‑1 (MQ=255)                                           gcgcAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTGCACGCACATCACGTGGGGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAGCCTCGAACACCTGAAGCGGGTGAATCAAATTGAGGCCTGGTTGAAGCAACCAGTGTCCATCGCTAATTTTCATTGCCGTTCCTTAGTTCTGCACTTCTTAAGAGGTGAATTGTTGCTGGCAACGGCGTTTACCCTGAGCCAGCTGTTCCATAACgg                                                                                                                                                                                              <  1:250898/251‑1 (MQ=255)                                                 cGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCACGGGGGGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTGAATCAAATTGAGGCCAGGTTGAATCAACCAGTTTCCATCGCTAATTTTCATTGCCGTTCCTTAGTTCTGCACTTCTTAAGAGGTGAATTGTTGCTGGCAACGGCGTTTACCCTGAGCCGGCTGTTCCATAACGGTTTTCa                                                                                                                                                                                        <  2:63432/251‑1 (MQ=255)                                                                       tGCCAGGAACGTTCACGCACATCACGGGGGGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTGAATCAAATTGAGGCCAGGTTGAATCAACCAGTTTCCATCGCTAATTTTCATTGCCGTTCCTTAGTTCTGCACTTCTTAAGAGGTGAATTGTTGCTGGCAACGGCGTTTACCCTGAGCCAGCTGTTCCATAACGGTTTTCAGATTGTGCGTCgtgagtgcgt                                                                                                                                                                   >  2:160061/1‑247 (MQ=255)                                                                               aCGTTCACGCACATAACGGGGGGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTGAATCAAATTGAGGCCAGGTTGAATCAACCAGTTTCCATCGCTAATTTTCATTGCCGTTCCTTAGTTCTGCACTTCTTAAGAGGTGAATTGTTGCTGGCAACGGCGTTTACCCTGAGCCAGCTGTTCCATAACGGTTTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGtt                                                                                                                                                          >  1:184864/1‑251 (MQ=255)                                                                                 gTTCACGCACATCACGGGGGGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTGAATCAAATTGAGGCCAGGTTGAATCAACCAGTTTCCATCGCTAATTTTCATTGCCGTTCCTTAGTTCTGCACTTCTTAAGAGGTGAATTGTTGCTGGCAACGGCGTTTACCCTGAGCCAGCTGTTCCATAACGGTTTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGttt                                                                                                                                                         >  1:193096/1‑250 (MQ=255)                                                                                                                              ccTGCTGTTCAACCTCGAACACCTGAAGCGGGTGAATCAAATTGAGGCCAGGTTGAATCAACCAGTTTCCATCGCTAATTTTCATTGCCGTTCCTTAGTTCTGCACTTCTTAAGAGGTGAATTGTTGCTGGCAACGGCGTTTACCCTGAGCCAGCTGTTCCATAACGGTTTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGATCGCCGGAACAATCGt                                                                                                            <  1:94962/250‑1 (MQ=255)                                                                                                                                    gTTCAACCTCGAACACCTGAAGCGGGTGAATCAAATTGAGGCCAGGTTGAATCAACCAGTTTCCATCGCTAATTTTCATTGCCGTTCCTTAGTTCTGCACTTCTTAAGAGGTGAATTGTTGCTGGCAACGGCGTTTACCCTGAGCCAGCTGTTCCATAACGGTTTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGATCGCCGGAACAATCGTGAATAgc                                                                                                     >  2:141905/1‑251 (MQ=255)                                                                                                                                          ccTCGAACACCTGAAGCGGGTGAATCAAATTGAGGCCAGGTTGAATCAACCAGTTTCCTGCGCTAATTTTCATGGCCGTTCCTTAGTTCTGCACTTCTTAAGAGGTGAATTGTTGCTGGCAACGGCGTTTACCCTGAGCCAGCTGTTCCATAACGGTTTTCAGATTGTGCGTCGTGAGGGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGATCGCCGGAACAATCGTGAATAGCGCaata                                                                                               <  2:207373/251‑1 (MQ=255)                                                                                                                                                acacCTGAAGCGGGTGAATCAAATTGAGGCCAGGTTGAATCAACCAGTTTCCATCGCTAATTTTCATTGCCGTTCCTTAGTTCTGCACTTCTTAAGAGGTGAATTGTTGCTGGCAACGGCGTTTACCCTGAGCCAGCTGTTCCATAACGGTGTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGATCGCCGGAACAATCGTGAATAGCGCAATAatgatg                                                                                         <  1:56563/251‑1 (MQ=255)                                                                                                                                                 cacCTGAAGCGGGTGAATCAAATTGAGGCCAGGTTGAATCAACCAGTTTCCATCGCTAATTTTCATTGCCGTTCCTTAGTTCTGCACTTCTTAAGAGGTGAATTGTTGCTGGCAACGGCGTTTACCCTGAGCCAGCTGTTCCATAACGGTTTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGATCGCCGGAACAATCGTGAATAGCGCAATAATGATGc                                                                                        >  1:82948/1‑251 (MQ=255)                                                                                                                                                        aGCGGGTGAATCAAATTGAGGCCAGGTTGAATCAACCAGTTTCCATCGCTAATTTTCATTGCCGTTCCTTAGTTCTGCACTTCTTAAGAGGTGAATTGTTGCTGGCAACGGCGTTTACCCTGAGCCAGCTGTTCCATAACGGTTTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGATCGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGt                                                                                 <  1:83223/251‑1 (MQ=255)                                                                                                                                                           gggTGAATCAAATTGAGGCCAGGTTGAATCAACCAGTTTCCATCGCTAATTTTCATTGCCGTTCCTTAGTTCTGCACTTCTTAAGAGGTGAATTGTTGCTGGCAACGGCGTTTACCCTGAGCCAGCTGTTCCATAACGGTTTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGATCGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGgc                                                                              >  1:101710/1‑251 (MQ=255)                                                                                                                                                                                           gcAGTTTCCATCGCTAATTTTAATTGCCGATCCTTAGTTCTGCACTTCTTAAGAGGAGAATTGTTGCTGGCAACGGCGTTTACCCTGAGCCAGCTGGTCAATAACGGTTTTAAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGATCGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATc                                              >  2:37296/2‑251 (MQ=255)                                                                                                                                                                                            cAGTTTCCATCGCTAATTTTCATTGCCGTTCCTTAGTTCTGCACTTCTTAAGAGGTGAATTGTTGCTGGCAACGGCGTTTACCCTGAGCCAGCTGTTCCATAACGGTTTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGATAGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATcc                                             >  2:17407/1‑251 (MQ=255)                                                                                                                                                                                                    aTCGCTAATTTTCATTGCCGTTACTTAGTTCTGCACTTCTTAAGAGGTGAATTGTTGCTGGCAACGGCGTTTACCCTGAGCCAGCTGTTCCATAACGGTTTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGATCGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATAGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATa                                       >  1:82304/1‑249 (MQ=255)                                                                                                                                                                                                                           cTTAGTTCTGAACTTCTTAAGAGGTGAATTGTTGCTGGCAACGGCGTTTACCCTGAGCCAGCTGTTCCATAACGGTTTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGATCGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAgcg              >  2:255087/1‑251 (MQ=255)                                                                                                                                                                                                                                     cACTTCTTAAGAGGTGAATTGTTGCTGGCAACGGCGTTTACCCTGAGCCAGCTGTTCCATAACGGTTTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGATCGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAgg    >  2:100626/1‑251 (MQ=255)                                                                                                                                                                                                                                         tcttAAGAGGTGAATTGTTGCGGGAAACGGCGTTTACCCGGAGCCAGCTGTTCCATAACGGTTTTCAGATTGGGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGAAAATAACAGATCGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGcc  <  1:141905/249‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                     CTCAATCCGCACACCGACAATACCTTCCTGTGGGGAGAAAAAGCGCAACGTAAATAAAGGCGTATCAAGCTGCCAGGTACGTTCACGCACATCACGGGGGGCAGCATAGACCACCATTTCATTATCCTGCTGTTCAACCTCGAACACCTGAAGCGGGTGAATCAAATTGAGGCCAGGTTGAATCAACCAGTTTCCATCGCTAATTTTCATTGCCGTTCCTTAGTTCTGCACTTCTTGAGAGGTGAATTGTTGCTGGCAACGGCGTTTACCCTGAGCCAGCTGTTCCATAACGGTTTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGATCGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCC  >  NC_000913/3834328‑3834808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑