| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,770,539 | C→T | V281M (GTG→ATG) | yibH ← | inner membrane protein YibH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,770,539 | 0 | C | T | 97.4% | 112.2 / NA | 38 | V281M (GTG→ATG) | yibH | inner membrane protein YibH |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (19/18); minor base G (0/1); total (19/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTCCGCATCACCGAAACATGGCTGAAATGGTCGGAGTAAACCGCCACCTGGGCGTAGATGCCGTCGGGTAAGGCATCGATATCATCGTTAGGGTCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACACCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTCAAGGTTATATTTTGCTTCAGTAAGTTGCGCTCTTAAGCTCACAATCTGCGATT > NC_000913/3770295‑3770781 | ttCCGCATCACCGAAACATGGCTGAAATGGTCGGAGTAAACCGCCACCTGGCCGTAGATGCCGTCGGGTAAGGCATCGATATCATCGTTAGGGTCCAGTTCAATGGTTCCCAGCACAACGTCCGTGCCGGGCACGACCGTTACTGATTGCAATACCCCCTGCGCCTGGTAAGAACCGCCTGGCACGACAGGAAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGCAGCGCGTTCACCATCACTTc > 2:79411/1‑251 (MQ=255) gccGCATCACCGAAACATGGCTGAAATGGTCGGAGTAAACCGCCACCTGGGCGTAGATGCCGTCGGGTAAGGCATCGATATCATCGTTAGGGTCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTc < 1:164436/249‑1 (MQ=255) tCACCGAAACATGGCTGAAATGGTCGGAGTAAACCGCCACCTGGGCGTAGATGCCGTCGGGTAAGGCATCGATATCATCGTTAGGGTCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAACCATAACTTCCGcatc > 2:119879/1‑250 (MQ=255) cACCGAAACATGGCTGCAATGGTCGGAGTAAACCGCCACCTGGGCGTAGATGCCGTCGGGTAAGGCATCGATATCATCGTTAGGGTCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGGAGCGCGTTAAACATCACTTCCGcatcct > 2:250087/1‑249 (MQ=255) ccGAAACATGGCTGAAATGGTCGGAGTAAACCGCCACCTGGGCGTAGATGCCGTCGGGTAAGGCATCGATATCATCGTTAGGGGCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCAGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCg > 2:80161/1‑251 (MQ=255) gggCGTAGATGCCGTCGGGTAAGTCATCGATATCATCGTTAGGGTCCAGTCCAATGGTTCCCAGAACACCGTCCGTTCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACAGCCCTTCCGCATCATCGCCAGTGTTCAGACGTCACAGCGAGTTTTGCCGAATTTgg < 2:228928/251‑1 (MQ=255) cGGGTAAGGCATCGATATCATCGTTAGGGTCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGTCCGTTAATGATTGCAATACCCCCTGCGCCGGATAAGAACCGCCTGGCACGACATGTAAAATACTAGTCAGTTTGCCGGGAAACTCCTGCCCAGGTAGCTCGTTAAACATCTCTTCCGCATCAGCGCCAGGTTTCTGACGTAACAGCTAGTTTTGCCTAAATTGGTCGATAATTTGCCg < 2:160281/250‑1 (MQ=255) ggTCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCAcc < 1:226589/251‑1 (MQ=255) cAGTTCCATGGTTCCCAGCACACCGGCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGCCAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCGGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGAc > 2:47411/1‑251 (MQ=255) cccccgtccgtGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGc < 1:104803/248‑1 (MQ=255) gCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCt < 1:147080/250‑1 (MQ=255) cACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGa < 1:119879/251‑1 (MQ=255) aCGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGAt < 1:158400/251‑1 (MQ=255) tGATTGCAATACGCCCTGCACCTGATATGCCCTGCATTGCACGACAGGTCAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGCAGCGCCTTCAACTTCACTTCCGCATCATCGCCAGGTTTCAGACGTCACAGCGAGTTTTGCCGACATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACCCCGCGGCACGGCGGCTGCGTCTGTACCTGGGCGGATCAGTACCTg > 2:15427/1‑249 (MQ=255) tGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGCCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTg > 2:130226/1‑249 (MQ=255) gATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGTCGGATCAGTACCTGAGt < 2:47746/251‑1 (MQ=255) gCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCGCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACg < 1:180782/251‑1 (MQ=255) cAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGCCAGGTAAACTACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGt > 2:49230/1‑251 (MQ=255) cTGCGCCTGATAAGCACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGATACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGGTTCAGACGTGACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGCTGACGACCATCACCGGACGCAGCGGCATGGCAGCTGCGTATGTACATGGGCGGATCAGTACCTGCGTAACGTAGCCCTTGc > 2:248416/1‑251 (MQ=255) tGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATAATAGTCAGTTTGCCGTGAAACACCTGACCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGAGGATCCGTACCTGTGTAACGTAGCCATTgat > 1:240207/1‑249 (MQ=255) ccTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGCAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATg > 1:134233/1‑207 (MQ=255) ccTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCATGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGGTTTTGCTCGGGGATGAAGACCATCACCTGACGCAGCGGCAAGGCAGCTGCGTATg < 2:134233/207‑1 (MQ=255) tGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGCGTAACGTAGCCATTGCTCGgcgc > 2:179300/1‑251 (MQ=255) aTAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGgcgcgc > 1:54686/1‑251 (MQ=255) aaGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTCGCCATTGCTCGGcgcgcg > 1:231926/1‑250 (MQ=255) ccTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGt < 1:123085/251‑1 (MQ=255) cGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTc > 2:103818/1‑250 (MQ=255) aGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGa > 1:196903/1‑146 (MQ=255) aGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGa < 2:196903/146‑1 (MQ=255) aaaaTACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGa > 2:173705/1‑139 (MQ=255) aaaaTACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGa < 1:173705/139‑1 (MQ=255) cccAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTCAAGGTTATATTTTGCTTCAGTAAGTTGCGCTCTTAAGCTc > 1:70169/1‑250 (MQ=255) aGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGCCGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTCAAGGTTATATTTTGCTTCAGTAAGTTGCGCTCTTAAGCTCACAATCTg < 1:80161/251‑1 (MQ=255) aGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTCAAGGTTATATTTTGCTTCAGTAAGTTGCGCTCTTAAGCTCACAATCt < 1:179300/250‑1 (MQ=255) aGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTCAAGGTTATATTTTGCTTCAGTAAGTTGCGCTCTTAAGCTCACAATCTg < 1:84581/251‑1 (MQ=255) gcgcGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTCAAGGTTATATTTTGCTTCAGTAAGTCGCGCTCTGAAGCTCACAATCTGc < 1:79411/251‑1 (MQ=255) gTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGCCAATTTGCCGTTTTTGCTCGGGGCTGAAGACCATCCCCGGCCGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTCACGGTTATATTTTGCTTCAGTAAGTTGCGCTCTTACGCTCACAATCTGCGAtt > 1:139096/1‑251 (MQ=255) gTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTCAAGGTTATATTTTGCTTCAGTAAGTTGCGCTCTTAAGCTCACAATCTGCGAtt < 1:13788/251‑1 (MQ=255) | TTCCGCATCACCGAAACATGGCTGAAATGGTCGGAGTAAACCGCCACCTGGGCGTAGATGCCGTCGGGTAAGGCATCGATATCATCGTTAGGGTCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACACCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTCAAGGTTATATTTTGCTTCAGTAAGTTGCGCTCTTAAGCTCACAATCTGCGATT > NC_000913/3770295‑3770781 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |