Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,665,576	C→T	V79I (GTT→ATT)	gadX ←	DNA‑binding transcriptional dual regulator GadX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,665,576	0	C	T	100.0%	26.8 / NA	12	V79I (GTT→ATT)	gadX	DNA‑binding transcriptional dual regulator GadX
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/8);  total (4/8)

TCATGGGCGATATTATTATTGATAACCGTACAAACTCGTGTTCGCATGTTCGGTTGTAAAACGTTCAGAAGCAGCGGTATAAAGTGCTCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCG‑TGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTA  >  NC_000913/3665338‑3665800                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                  tCATGGGCGATATTATTATTGATAACCGTACAAACTCGTGTTCGCATGTTCGGTTGTAAAACGTTCAGAAGCAGCGGTATAAAGTGCTCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAACAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATTTTTTTAtgtg                                                                                                                                                                                                                        >  1:1850/1‑250 (MQ=255)   atgtgCGATAGTATTATTGATAACCGGACAAACTCGTGGTCGCATGTTCGGTTGTAAAACGTTCAGAAGCAGCGGTATAAAGTGCTCATCCTCAATAAAAACAGATAACAACGCAAAGATTAAGTCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATGCTGCGATAGTTGCGCAACTTCCTGAAAAACATGAATATCGCTACTACAGTGATGAACTGGTAATGAAGAAATTGTGTTATGTGCa                                                                                                                                                                                                                      <  2:178369/246‑1 (MQ=255)                    tGATAACCGTACAAACTCGTGTTCGCATGTTCGGTTGTAAAACGTTCAGAAGCAGCGGTATAAAGTGCTCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATTTTTTTATGTGCATGAAGATTTAATGcctc                                                                                                                                                                                                     <  2:98756/250‑1 (MQ=255)                                                                                                               caacGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAAGGAAGAAATTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCG‑TGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCAt                                                                                                        <  1:99852/251‑1 (MQ=255)                                                                                                                                 tctTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCG‑TGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCAc                                                                                      >  1:6588/1‑251 (MQ=255)                                                                                                                                    tttACGTAGCATTACTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCG‑TGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCAc                                                                                      <  2:80102/248‑1 (MQ=255)                                                                                                                                         gTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCG‑TGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCAcc                                                                                     >  1:207814/1‑244 (MQ=255)                                                                                                                                         gTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCG‑TGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCAcc                                                                                     <  2:207814/244‑1 (MQ=255)                                                                                                                                          tAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATTTTTATATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCG‑TGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTa                                                                              >  1:267303/1‑250 (MQ=255)                                                                                                                                                                                  ggcgcAACTTCCTAAAAAACAGAAATATCTCTACAACATTGATGAACGAGTAATGAAAAAATTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTGGAGAATCGGAAAAAATAA‑GAAAGGGGTGTCCCTTGACACGAATACAAAATTTCAAACACCCTTATCTACTCGAATTTGGCTTGAACCCGAAAAACCCAGTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAtt                                      <  1:229224/249‑1 (MQ=255)                                                                                                                                                                                        ccTTCCTAAAAAACAGAAATATCTCTACAAGAGTGATGAACGAGTAATGAAAAATTTTTTTTAGGGGCATGAATATTTAATGCCTCCCCCTGTAGAGTGGGGAGAAATAAGAAAAGGG‑TGTCCCTTGACACAAAAAAAAAAGTTTCAACACTTTTATCTCCGGGATTTTGGCTTCCATCCGCAAAAACCTGGTAACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCt                               <  1:252489/250‑1 (MQ=255)                                                                                                                                                                                                                     aaattgataaagagtaaaTGAAAAAATTTTTTTATGTGAAGAAAAATTTAGGCCCTCCTCCTTGAGAATCGGGAAAAAAAATGAAAGCT‑TGTCCCTTGACACGAATAAAAAATTTTCAACACCCTTCTCTACGAGATTTGGGCTTCAATCCAAAAAAACCAGTGCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTa  <  1:18885/235‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                  TCATGGGCGATATTATTATTGATAACCGTACAAACTCGTGTTCGCATGTTCGGTTGTAAAACGTTCAGAAGCAGCGGTATAAAGTGCTCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCG‑TGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTA  >  NC_000913/3665338‑3665800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑