Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,664,594	G→A	L9F (CTC→TTC)	gadW ←	DNA‑binding transcriptional dual regulator GadW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,664,594	0	G	A	100.0%	48.9 / NA	18	L9F (CTC→TTC)	gadW	DNA‑binding transcriptional dual regulator GadW
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/9);  total (9/9)

AGCGGCATGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACAAAACGCAAAATCGTCTGCCAAATTTTTCTCCAGCAGCAGAATACTCTCGTTATGCAGCGATATTTTTTGCTGTTCATGATAAATATCGAATGAACGACGAATGAGGATCACCGAGCAGACATGAGTCATGATTATCCCTTATATTTCATACTGCGATTATTTCAATTTCAGTATACTAATGAAATGATGCCAGACTGTTTCTTAGCGCAAAAACTGCCAGATTTGGTAGGACCAAAATTTATCCACAAAGTAGTTTTGCATAACAGTTAATTGATAATAAGTAAATGCAGCACGAATATATTTTCGCACAGCGTATAGCTTATGTTTATAAAAAAATGGCTGATCTTAT  >  NC_000913/3664350‑3664838                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     aGCGGCATGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACAAAACGCAAAATCGTCTGCCAAATTTTTCTCCAGCAGCAGAATACTCTCGTTATGCAGCGATATTTTTTGCTGTTCATGATAAATATCGAATGAACGACGAATGAAGATCAc                                                                                                                                                                                                                                                <  1:167410/251‑1 (MQ=255)      cATGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTCATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACAAAACGCAAAATCGTCTGCCAAATTTTTCTCCAGCAGCAGAATACTCTCGTTATGCAGCGATATTTTTTGCTGTTCATGATAAATATCGAATGAACGACGAATGAAGATCACCGAGc                                                                                                                                                                                                                                           >  1:160697/1‑251 (MQ=255)       aTGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTTAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACAAAACGCAAAATCGTCTGCCAAATGTTTCTCCAGCAGCAGAATACTCTCGTTATGCAGCGATATTTTTTGCTGTTCATGATAAATATCGAATGAACGACGAATGAAGATCACCGAGCa                                                                                                                                                                                                                                          <  1:132163/251‑1 (MQ=255)                                                   cccTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACAAAACGCAAAATCGTCTGCCAAATTTTTCTCCAGCAGCAGAATACTCTCGTTATGCAGCGATATTTTTTGCTGTTCATGATAAATATCGAATGAACGACGAATGAAGATCACCGAGCAGACATGAGTCATGATTATCCCTTATATTTCATACTGCGattatt                                                                                                                                                                                              >  2:152066/1‑251 (MQ=255)                                                                                tttGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACAAAACGCAAAATCGTCTGCCAAATTTTTCTCCAGCAGCAGAATACTCTCGTTATGCAGCGATATTTTTTGCTGTTCATGATAAATATCGAATGAACGACGAATGAAGATCACCGAGCAGACATGAGTCATGATTATCCCTTATATTTCATACTGCGATTATTTCAATTTCAGTATACTAATGAAatgatg                                                                                                                                                                  <  1:150731/250‑1 (MQ=255)                                                                                                  cTGTCCGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACAAAACGCAAAATCGTCTGCCAAATTTTTCTCCAGCAGCAGAATACTCTCGTTATGCAGCGATATTTTTTGCTGTTCATGATAAATATCGAATGAACGACGAATGAAGATc                                                                                                                                                                                                                                                  >  2:81788/1‑152 (MQ=255)                                                                                                  cTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACAAAACGCAAAATCGTCTGCCAAATTTTTCTCCAGCAGCAGAATACTCTCGTTATGCAGCGATATTTTTGGCTGTTCATGATAAATATCGAATGAACGACGAATGAAGATc                                                                                                                                                                                                                                                  <  1:81788/152‑1 (MQ=255)                                                                                                                atatCCAGTCGTCGCGTATCCGGTGAACAAAACGCAAAATCGTCTGCCAAATTTTTCTCCAGCAGCAGAATACTCTCGTTATGCAGCGATATTTTTTGCTGTTCATGATAAATATCGAATGAACGACGAATGAAGATCACCGAGCAGACATGAGTCATGATTATCCCTTATATTTCATACTGCGATTATTTCAATTTCAGTATACTAATGAAATGATGCCAGACTGTTTCTTAGCGCAAAAACTGcc                                                                                                                                     >  1:233868/1‑247 (MQ=255)                                                                                                                  atCCAGTCGTCGCGTATCCGGTGAACAAAACGCAAAATCGTCTGCCAAATTTTTCTCCAGCAGCAGAATACTCTCGTTATGCAGCGATATTTTTTGCTGTTCATGATAAATATCGAATGAACGACGAATGAAGATCACCGAGCAGACATGAGTCATGATTATCCCTTATATTTCATACTGCGATTATTTCAATTTCAGTATACTAATGAAATGATGCCAGACTGTTTCTTAGCGCAAAAACTGCCAGAtt                                                                                                                                <  1:90481/250‑1 (MQ=255)                                                                                                                                       tGAACAAAACGCAAAATCGTCTGCCAAATTTTTCTCCAGCAGCAGAATACTCTCGTTATGCAGCGATATTTTTTGCTGTTCATGATAAATATCGAATGAACGACGAATGAAGATCACCGAGCAGACATGAGTCATGATTATCCCTTATATTTCATACTGCGATTATTTCAATTTCAGTATACTAATGAAATGATGCCAGACTGTTTCTTAGCGCAAAAACTGCCAGATTTGGTAGGACCAAAATTTATcc                                                                                                           >  1:59629/1‑250 (MQ=255)                                                                                                                                        gAACAAAACGCAAAATCGTCTGCCAAATTTTTCTCCAGCAGCAGAATACTCTCGTTATGCAGCGATATTTTTTGCTGTTCATGATAAATATCGAATGAACGACGAATGAAGATCACCGAGCAGACATGAGTCATGATTATCCCTTATATTTCATACTGCGATTATTTCAATTTCAGTATACTAATGAAATGATGCCAGACTGTTTCTTAGCGCAAAAACTGCCAGATTTGGTAGGACCAAAATTTATCcac                                                                                                         <  2:233868/251‑1 (MQ=255)                                                                                                                                                          tCTGCCAAATTTTTCTCCAGCAGCAGAATACTCTCGTTATGCAGCGATATTTTTTGCTGTTCATGATAAATATCGAATGAACGACGAATGAAGATCACCGAGCAGACATGAGTCATGATTATCCCTTATATTTCATACTGCGATTATTTCAATTTCAGTATACTAATGAAATGATGCCAGACTGTTTCTTAGCGCAAAAACTGCCAGATTTGGTAGGACCAAAATTTATCCACAAAGTAGTTTTGCATAAc                                                                                       >  1:133523/1‑251 (MQ=255)                                                                                                                                                                                          ctcGTTATGCAGCGATATTTTTTGCTGTTCATGATAAATATCGAATGAACGACGAATGAAGATCACCGAGCAGACATGAGGCATGATTATCCCTTAGATTTCATACTGCGATTATTTCAATTTCATTATACTAATGAAATGATGCCAGACTGTTTCTTAGCGCAAAAACGGCCAGATTTGGTAGGACCAAAATTTATCCACAAAGTAGTTTTGCTTAACAGTTAGTTGATAATAAGTAAAGGCAGCACg                                                         <  1:239859/249‑1 (MQ=255)                                                                                                                                                                                             gTTATGCAGCGATATTTTTTGCTGTTCATGATAAATATCGAATGAACGACGAATGAAGATCACCGAGCAGACATGAGTCATGATTATCCCTTATATTTCATACTGCGATTATTTCAATTTCAGTATACTAATGAAATGATGCCAGACTGTTTCTTAGCGCAAAAACTGCCAGATTTGGTAGGACCAAAATTTATCCACAAAGTAGTTTTGCATAACAGTTAATTGATAATAAGTAAATGCAGCACGAatat                                                    <  2:133523/251‑1 (MQ=255)                                                                                                                                                                                                aTGCAGCGATATTTTTTGCTGTTCATGATAAATATCGAATGAACGACGAATGAAGATCACCGAGCAGACATGAGTCATGATTATCCCTTATATTTCATACTGCGATTATTTCAATTTCAGTATACTAATGAAATGATGCCAGACTGTTTCTTAGCGCAAAAACTGCCAGATTTGGTAGGACCAAAATTTATCCACAAAGTAGTTTTGCATAACAGTTAATTGATAATAAGTAAATGCAGCACGAATATAtt                                                 >  2:132645/1‑251 (MQ=255)                                                                                                                                                                                                          atTTTTTGCTGTTCATGATAAATATCGAATGAACGACGAATGAAGATCACCGAGCAGACATGAGTCATGATTATCCCTTATATTTCATACTGCGATTATTTCAATTTCAGTATACTAATGAAATGATGCCAGACTGTTTCTTAGCGCAAAAACTGCCAGATTTGGTAGGACCAAAATTTATCCACAAAGTAGTTTTGCATAACAGTTAATTGATAATAAGTAAATGCAGCACGAATATATTTTCGCACAg                                        <  1:165969/250‑1 (MQ=255)                                                                                                                                                                                                                           ataaatatggcctGAACGACGAATGAAGAACACCGGGCAGACATGAGTCGTGCTTATCCATTATATTTCATACTGCGATTATTTCAATTTCAGTATACTAATGAAATGATGCCAGACTGTTTATTCGCGCAAAAACTGCCAGATTTGGTAGGACCAAAATTTATCCACAAAGTAGTTTTGAATAACAGTTAATTGATAATAAGTAAATGCAGCACGAATATATTTTCGCACAGCGTATAGCTTATGTTtat                      >  2:160049/13‑251 (MQ=255)                                                                                                                                                                                                                                               gaATGAAGATCACCGAGCAGACATGAGTCATGATTATCCCTTATATTTCATAATGCGATTATTTCAATTTCAGTATACTACTGAAATGCTGCCAGACTGTTTATTAGCGCAAAAACTGCCAGCTTTGGTAGGACCAAAATTTATCCACAAAGTAGTTTTGCATAACAGTTAATTGATAATAAGTAAATGCAGCCCGAATATATTTTCGCACAGCGTATAGATTATGTTTATAAAAAAATGGCTGATCTTAt  >  1:250074/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     AGCGGCATGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACAAAACGCAAAATCGTCTGCCAAATTTTTCTCCAGCAGCAGAATACTCTCGTTATGCAGCGATATTTTTTGCTGTTCATGATAAATATCGAATGAACGACGAATGAGGATCACCGAGCAGACATGAGTCATGATTATCCCTTATATTTCATACTGCGATTATTTCAATTTCAGTATACTAATGAAATGATGCCAGACTGTTTCTTAGCGCAAAAACTGCCAGATTTGGTAGGACCAAAATTTATCCACAAAGTAGTTTTGCATAACAGTTAATTGATAATAAGTAAATGCAGCACGAATATATTTTCGCACAGCGTATAGCTTATGTTTATAAAAAAATGGCTGATCTTAT  >  NC_000913/3664350‑3664838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑